Scientia Agricultura Sinica ›› 2025, Vol. 58 ›› Issue (1): 156-169.doi: 10.3864/j.issn.0578-1752.2025.01.012

• HORTICULTURE • Previous Articles     Next Articles

Cloning and Functional Analysis of the Autophagy Gene ATG8f in the Grapevine

GE Yi1,2(), ZHENG QiuLing3,4, CHEN MengXia1,2, XIA JiaXin1,2, FANG Xiang2,5,*(), TANG MeiLing3,4, FANG JingGui1,2,6, SHANGGUAN LingFei1,2,6,*()   

  1. 1 Department of Horticulture, Nanjing Agricultural University, Nanjing 210095
    2 Fruit Crop Genetic Improvement and Seedling Propagation Engineering Research Center of Jiangsu Province, Nanjing 210095
    3 Yantai Academy of Agricultural Sciences in Shandong Province, Yantai 264003, Shandong
    4 Yantai Key Laboratory of Quality Safety and Nutrition of Characteristic Fruits, Yantai 264003, Shandong
    5 School of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Jurong 212400, Jiangsu
    6 Collaborative Innovation Center for Wine Industry Technology of Ningxia Helan Mountain Eastern Foothills, Yinchuan 750299
  • Received:2024-06-04 Accepted:2024-07-30 Online:2025-01-01 Published:2025-01-07
  • Contact: FANG Xiang, SHANGGUAN LingFei

RichHTML

29

PDF

97

Abstract:

【Objective】This study aims to investigate the role of the grape autophagy gene ATG8f in regulating copper stress tolerance in plants, thereby laying the foundation for elucidating copper tolerance mechanisms and constructing regulatory networks. 【Method】Based on multi-omics sequencing analysis of Shine Muscat grapevine under copper stress, the autophagy gene VlATG8f was identified and its sequence characteristics were analyzed. The CDS sequence of VlATG8f was cloned and an overexpression vector was constructed. The subcellular localization of the VlATG8f protein was studied through transient expression in tobacco. Heterologous expression of VlATG8f in Arabidopsis was achieved using the floral dip method. Seed germination rate and root length were measured to compare the growth and development of wild-type and transgenic lines under copper stress. Real-time quantitative PCR was used to analyze the expression levels of related antioxidant genes, and yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays were used to preliminarily screen for VlATG8f- interacting proteins. 【Result】The CDS of VlATG8f was 393 bp, encoding 130 amino acids. Bioinformatics analysis revealed that the ATG8f protein sequences in Arabidopsis, tomato, apple, pear, peach, and grapevine were relatively conserved, all containing a Ubl_ATG8 conserved domain, indicating potential functional were conserved. Subcellular localization showed that the VlATG8f protein was located on both the nucleus and cell membrane. Under normal conditions, both wild-type and VlATG8f-overexpressing lines exhibited healthy growth with green leaves. Under copper stress, the transgenic lines were less stunted and exhibited milder symptoms of chlorosis, leaf curling, reduced root hair size, and increased lateral roots compared to wild-type plants. Overexpression of VlATG8f in Arabidopsis resulted in less growth inhibition under copper stress, higher seed germination rate, longer root, and lower accumulation of hydrogen peroxide (H2O2), indicating enhanced copper tolerance. Additionally, the expression of antioxidant genes AtCSD1 and AtCCS1 increased in VlATG8f-overexpressing plants under copper stress, while the expression of the key ROS synthesis gene RbohB decreased, promoting ROS scavenging. Yeast two-hybrid screening identified 23 potential interacting proteins with VlATG8f. BiFC further confirmed strong interactions between VlATG8f and proteins such as PYL8, MnSOD, AUX22D, bZIP17, and COT44. 【Conclusion】Overexpression of VlATG8f may enhance copper tolerance in transgenic lines by strengthening the antioxidant system, reducing ROS synthesis, and promoting the synthesis of plant hormones, thereby mitigating copper toxicity in plants.

Key words: grapevine, autophagy, copper stress, VlATG8f, antioxidant-related genes

Table 1

The primers used for this experiment"

引物名称
Primers name		 正向引物
Forward primers (5′-3′)		 反向引物
Reverse primers (5′-3′)		 用途
Application
VlATG8f-F/R TTTGCTGATGAAGGAGGAATGGC TCGACAATCACCGGAATCCTGT 拟南芥定量引物
Arabidopsis thaliana quantitative primers
AtRbohB-F/R CCGGGGATGATTACCTCAGC CGATGTCAATGCCGCTCTTG
AtGPX6-F/R CAAAGCTGCCCCAGTCTACA GGTGAGGTAGTTGGTGCGAA
AtCSD1-F/R CCCTGAGGATGCTAATCGACAT TGGCAATCAGTGATTGTGAAGG
AtTRX1-F/R ACATGGAACGAGCAGCTTCA GCCTGTATCGCCCAATCACT
AtCCS1-F/R GCAAACTGGTCGAAAAGCTC GTCAGGGCCTTTGAATTCTG
AtP5CS1-F/R GCAGCTTTGCGGATCTTCAG GATATGGGGCTCTTCGGGTG
AtMDAR4-F/R GGAATGCGGTTGTTATCGGC ACCAAGTCGGCTGGTAAGTG
AtPEX4-F/R CTCCTTACGAAGGCGGTGTT TTCTTAGGCATAGCGGCGAG 拟南芥定量引物（Actin）
Arabidopsis thaliana quantitative primers
Hyg-F/R GGTCGCGGAGGCTATGGATGC GCTTCTGCGGGCGATTTGTGT 转基因拟南芥鉴定
Identification of transgenic Arabidopsis thaliana
1302-VlATG8f GAAGATCTATGATTTTTGCTGATGAAGGAGGA GGACTAGTATGTGGTATCTCATATCCAAACGT 构建植物过表达载体
Construction of plant overexpression vectors
1305-VlATG8f AGGACAGCCCAGATCACTAGTATGATTTTTGCTGATGAAGGAGGA GCTCACCATGGATCCCCCGGGATGTGGTATCTCATATCCAAACGT 构建亚细胞定位载体
Construction of subcellular localized vectors
BD-VlATG8f ATGGCCATGGAGGCCGAATTCATGATTTTTGCTGATGAAGGAGGA CCGCTGCAGGTCGACGGATCCATGTGGTATCTCATATCCAAACGT 构建BD载体
Construction of the BD vector

Fig. 1

Observation of autophagy in grapevine leaves under high-ECS and the expression levels of related gene and protein A: Observation of autophagosomes by transmission electron microscopy. Arrows indicate autophagosomes, Bar=2 μm; B: Counts of the number of autophagosomes; C: Expression levels of autophagy genes under high copper stress; D: ATG8f protein content under high-ECS. Different lowercase letters indicate significant differences at the P<0.05 level. The same as below"

Fig. 2

The bioinformatic analysis of VlATG8f and its subcellular localization A: Phylogenetic tree analysis; B: Homology analysis. VlATG8f was marked with an asterisk; C: Subcellular localization. At the far left of images show cells with green GFP signal. mCherry was red mCherry signal. Light was bright-field images of the same cells, merged images of a cinfocal fluorescence image and a bright field image. The red triangles point to the nucleus. Bar=50 μm"

Fig. 3

Screening and identification of Arabidopsis overexpression lines A and B: Selection of overexpressing transgenic plants; C: RT-qPCR analysis of overexpressed transgenic plants"

Fig. 4

Phenotypes of WT and transgenic Arabidopsis overexpression lines under Cu stress A: Phenotypes of different lines; B: The statistical analysis of germination rate; C: The statistical analysis of root length"

Fig. 5

Effect of overexpression of VlATG8f on the antioxidant system A: DAB staining results; B: Expression levels of antioxidant-related genes in overexpressed VlATG8f lines"

Fig. 6

Screening and validation of the candidate VlATG8f interaction proteins A: Toxicity and transcriptional activation detection assays of VlATG8f protein; B: Y2H validation of proteins interacting with VlATG8f; C: BiFC assay of VlATG8f interaction candidate proteins in B. The red triangles were represented the nuclei of cells. An irregular polygon surrounded by a yellow curve was the cell membrane. Bar=20 μm"

Table 2

The screened candidate interaction proteins of VlATG8f"

编号
Code		 基因
Gene		 蛋白ID
Protein ID		 描述
Description
1 VvDRIP2 XP_010651942.1 E3泛素蛋白连接酶E3 ubiquitin protein ligase DRIP2
2 VvsGR1 XP_002282183.1 滞绿蛋白1，叶绿体Protein STAY-GREEN 1, chloroplastic
3 VvPsaD XP_002281825.1 光系统Ⅰ反应中心亚基Ⅱ，叶绿体Photosystem Ⅰ reaction center subunit Ⅱ, chloroplastic
4 VvGST XP_002262842.1 谷胱甘肽S-转移酶Glutathione S-transferase
5 VvRHF2a XP_010653244.1 E3泛素蛋白连接酶RHF2A亚型X2 E3 ubiquitin-protein ligase RHF2A isoform X2
6 VvAUX22D XP_002281696.1 生长素诱导蛋白22D Auxin-induced protein 22D
7 VvPYL8 XP_010659134.1 脱落酸受体PYL8 Abscisic acid receptor PYL8
8 VvHIPP39 XP_002274512.1 重金属相关异戊二烯化植物蛋白39 Heavy metal-associated isoprenylated plant protein 39
9 VvbZIP17 XP_010653644.1 bZIP转录因子17 bZIP transcription factor 17
10 VvGOLS1 XP_002265947.1 半乳糖醇合酶1 Galactinol synthase 1
11 VvCOT44 XP_002283263.1 半胱氨酸蛋白酶COT44 Cysteine proteinase COT44
12 VvsDH6 XP_002268190.1 琥珀酸脱氢酶亚基6，线粒体Succinate dehydrogenase subunit 6, mitochondrial
13 VvALS1 XP_002274073.1 乙酰乳酸合酶1，叶绿体Acetolactate synthase 1, chloroplastic
14 VvPGDH3 XP_002273552.1 D-3-磷酸甘油酸脱氢酶3，叶绿体D-3-phosphoglycerate dehydrogenase 3, chloroplastic
15 VvEXO XP_002285762.1 磷酸诱导酶Phosphoinductase 1
16 VvPP6-ARS-C XP_002268442.1 丝氨酸/苏氨酸蛋白磷酸酶6调节锚蛋白重复亚基C
Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C
17 VvDRM homolog 4 XP_003632757.1 休眠相关蛋白同源物4亚型X2 Dormancy-associated protein homolog 4 isoform X2
18 VvOEE1 XP_002274796.1 放氧增强蛋白1，叶绿体Oxygen-evolving enhancer protein 1, chloroplastic
19 VvFKBP13 XP_002272628.1 肽基脯氨酰顺反异构酶FKBP13，叶绿体Peptidyl-prolyl cis-trans isomerase FKBP13, chloroplastic
20 VvMnSOD NP_001268135.1 锰超氧化物歧化酶Manganese superoxide dismutase
21 VvVAMP711 XP_002285207.1 囊泡相关膜蛋白711 Vesicle-associated membrane protein 711
22 VvPAT2 XP_019072271.1 蛋白驱动蛋白轻链相关2 Protein kinesin light chain-related 2
23 VvHCF136 XP_002278958.1 光系统Ⅱ稳定性/组装因子HCF136，叶绿体
Photosystem Ⅱ stability/assembly factor HCF136, chloroplastic
[1]
YRUELA I. Copper in plants: Acquisition, transport and interactions. Functional Plant Biology, 2009, 36(5): 409-430.

doi: 10.1071/FP08288 pmid: 32688656
[2]
LI L, ZHANG K N, GILL R A, ISLAM F, FAROOQ M A, WANG J, ZHOU W J. Ecotoxicological and interactive effects of copper and chromium on physiochemical, ultrastructural, and molecular profiling in Brassica napus L. BioMed Research International, 2018, 2018: 9248123.
[3]
MARSHALL R S, VIERSTRA R D. Autophagy: The master of bulk and selective recycling. Annual Review of Plant Biology, 2018, 69: 173-208.

doi: 10.1146/annurev-arplant-042817-040606 pmid: 29539270
[4]
CAO J J, ZHOU J. Functions of plant autophagy and its applications in agriculture. Scientia Sinica Vitae, 2023, 53(3): 304-321.
[5]
MASCLAUX-DAUBRESSE C, CHEN Q W, HAVÉ M. Regulation of nutrient recycling via autophagy. Current Opinion in Plant Biology, 2017, 39: 8-17.
[6]
AHMAD P, JALEEL C A, SALEM M A, NABI G, SHARMA S. Roles of enzymatic and nonenzymatic antioxidants in plants during abiotic stress. Critical Reviews in Biotechnology, 2010, 30(3): 161-175.

doi: 10.3109/07388550903524243 pmid: 20214435
[7]
GENG A J, WANG X, WU L S, WANG F H, WU Z C, YANG H, CHEN Y, WEN D, LIU X X. Silicon improves growth and alleviates oxidative stress in rice seedlings (Oryza sativa L.)by strengthening antioxidant defense and enhancing protein metabolism under arsanilic acid exposure. Ecotoxicology and Environmental Safety, 2018, 158: 266-273.
[8]
NIETO-TORRES J L, LEIDAL A M, DEBNATH J, HANSEN M. Beyond autophagy: The expanding roles of ATG8 proteins. Trends in Biochemical Sciences, 2021, 46(8): 673-686.
[9]
CHEN X G, HE Y X, WU Z, LU X K, YIN Z J, ZHAO L J, HUANG H, MENG Y, FAN Y P, GUO L X, WANG D L, WANG J J, WANG S, CHEN C, WANG X P, YE W W. Systematic analysis and expression of Gossypium ATG8 family reveals the roles of GhATG8f responding to salt stress in cotton. Plant Cell Reports, 2024, 43(2): 58.
[10]
JIA X, JIA X M, LI T T, WANG Y, SUN X, HUO L Q, WANG P, CHE R M, GONG X Q, MA F W. MdATG5a induces drought tolerance by improving the antioxidant defenses and promoting starch degradation in apple. Plant Science, 2021, 312: 111052.
[11]
WANG P, SUN X, JIA X, WANG N, GONG X Q, MA F W. Characterization of an autophagy-related gene MdATG8i from apple. Frontiers in Plant Science, 2016, 7: 720.
[12]
LI W W, CHEN M, ZHONG L, LIU J M, XU Z S, LI L C, ZHOU Y B, GUO C H, MA Y Z. Overexpression of the autophagy-related gene SiATG8a from foxtail millet (Setaria italica L.)confers tolerance to both nitrogen starvation and drought stress in Arabidopsis. Biochemical and Biophysical Research Communications, 2015, 468(4): 800-806.
[13]
LI B, LIU G Y, WANG Y Q, WEI Y X, SHI H T. Overexpression of banana ATG8f modulates drought stress resistance in Arabidopsis. Biomolecules, 2019, 9(12): 814.
[14]
CHEN M X, FANG X, WANG Z C, SHANGGUAN L F, LIU T H, CHEN C, LIU Z J, GE M Q, ZHANG C, ZHENG T, FANG J G. Multi-omics analyses on the response mechanisms of ‘Shine Muscat’ grapevine to low degree of excess copper stress (Low-ECS). Environmental Pollution, 2021, 286: 117278.
[15]
陈梦霞. 葡萄叶片轻度铜胁迫调控机制研究与VvATG8f基因的功能分析[D]. 南京: 南京农业大学, 2021.
CHEN M X. Study on the regulation mechanism of mild copper stress in grape leaves and functional analysis of VvATG8f[D]. Nanjing: Nanjing Agricultural University, 2021. (in Chinese)
[16]
CLOUGH S J, BENT A F. Floral dip: A simplified method for Agrobacterium‐mediated transformation of Arabidopsis thaliana. The Plant Journal, 1998, 16(6): 735-743.
[17]
LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods, 2001, 25(4): 402-408.
[18]
熊桂红, 胡昱颛, 吴祖建. 利用酵母双杂交系统筛选本氏烟中与RGSV P5互作的蛋白. 江西农业大学学报, 2023, 45(2): 404-412.
XIONG G H, HU Y Z, WU Z J. Screening of RGSV P5 interactive proteins in Nicotiana benthamiana by two-hybrid system of yeast. Acta Agriculturae Universitatis Jiangxiensis, 2023, 45(2): 404-412. (in Chinese)
[19]
SUN X, WANG P, JIA X, HUO L Q, CHE R M, MA F W. Improvement of drought tolerance by overexpressing MdATG18a is mediated by modified antioxidant system and activated autophagy in transgenic apple. Plant Biotechnology Journal, 2018, 16(2): 545-557.
[20]
BASSHAM D C, LAPORTE M, MARTY F, MORIYASU Y, OHSUMI Y, OLSEN L J, YOSHIMOTO K. Autophagy in development and stress responses of plants. Autophagy, 2006, 2(1): 2-11.

doi: 10.4161/auto.2092 pmid: 16874030
[21]
HAYWARD A P, DINESH-KUMAR S P. What can plant autophagy do for an innate immune response? Annual Review of Phytopathology, 2011, 49: 557-576.

doi: 10.1146/annurev-phyto-072910-095333 pmid: 21370973
[22]
NAKATOGAWA H, ICHIMURA Y, OHSUMI Y. Atg8, a ubiquitin- like protein required for autophagosome formation, mediates membrane tethering and hemifusion. Cell, 2007, 130(1): 165-178.
[23]
XIE Z P, NAIR U, KLIONSKY D J. Atg 8 controls phagophore expansion during autophagosome formation. Molecular Biology of the Cell, 2008, 19(8): 3290-3298.
[24]
冷翔鹏. 葡萄应答铜胁迫的分子机理研究[D]. 南京: 南京农业大学, 2015.
LENG X P. Study on the molecular mechanism of grape in response to copper stress[D]. Nanjing: Nanjing Agricultural University, 2015. (in Chinese)
[25]
赵慧, 赵一博, 辛翠花, 郭江波. 过表达细胞自噬基因ATG8f对植物响应镉胁迫的影响. 分子植物育种, 2017, 15(8): 3042-3045.
ZHAO H, ZHAO Y B, XIN C H, GUO J B. Impacts on cadmium stress susceptibility in plants overexpressing autophagy-related gene ATG8f. Molecular Plant Breeding, 2017, 15(8): 3042-3045. (in Chinese)
[26]
PATEL S, CAPLAN J, DINESH-KUMAR S P. Autophagy in the control of programmed cell death. Current Opinion in Plant Biology, 2006, 9(4): 391-396.

pmid: 16713731
[27]
肖旭峰, 李猛, 司舒成, 范淑英, 吴才君, 张明. 基于转录组测序的Cd胁迫下芹菜细胞自噬相关基因的筛选. 生物工程学报, 2020, 36(8): 1610-1619.
XIAO X F, LI M, SI S C, FAN S Y, WU C J, ZHANG M. Effect of Cd on autophagy-related genes of celery. Chinese Journal of Biotechnology, 2020, 36(8): 1610-1619. (in Chinese)
[28]
田丽娟. 小麦ATG8基因在植物抵抗逆境胁迫中的功能研究[D]. 保定: 河北农业大学, 2014.
TIAN L J. Study on the function of wheat TaATG8 gene in plant resistance to stress[D]. Baoding: Hebei Agricultural University, 2014. (in Chinese)
[29]
SUNKAR R, LI Y F, JAGADEESWARAN G. Functions of microRNAs in plant stress responses. Trends in Plant Science, 2012, 17(4): 196-203.

doi: 10.1016/j.tplants.2012.01.010 pmid: 22365280
[30]
JING X S, HOU P C, LU Y J, DENG S R, LI N Y, ZHAO R, SUN J, WANG Y, HAN Y S, LANG T, DING M Q, SHEN X, CHEN S L. Overexpression of copper/zinc superoxide dismutase from mangrove Kandelia candel in tobacco enhances salinity tolerance by the reduction of reactive oxygen species in chloroplast. Frontiers in Plant Science, 2015, 6: 23.
[31]
WU J H, ZHANG J, LI X, XU J J, WANG L. Identification and characterization of a PutCu/Zn-SOD gene from Puccinellia tenuiflora (Turcz.)Scribn. et Merr. Plant Growth Regulation, 2016, 79(1): 55-64.
[32]
PARK S Y, GRABAU E. Bypassing miRNA-mediated gene regulation under drought stress: Alternative splicing affects CSD1 gene expression. Plant Molecular Biology, 2017, 95(3): 243-252.
[33]
管彬, 周竹青. 细胞自噬在拟南芥应答镉胁迫中的作用. 江苏农业科学, 2019, 47(14): 90-95.
GUAN B, ZHOU Z Q. Role of autophagy in response to cadmium stress in Arabidopsis thaliana. Jiangsu Agricultural Sciences, 2019, 47(14): 90-95. (in Chinese)
[34]
XIA J X, WANG Z C, LIU S Y, FANG X, HAKEEM A, FANG J G, SHANGGUAN L F. VvATG6 contributes to copper stress tolerance by enhancing the antioxidant ability in transgenic grape calli. Physiology and Molecular Biology of Plants, 2024, 30(1): 137-152.
[35]
张静娴, 刘军, 周晓慧, 刘松瑜, 庄勇, 杨艳. 茄子自噬相关基因ATG8家族鉴定及表达分析. 西北植物学报, 2023, 43(10): 1621-1628.
ZHANG J X, LIU J, ZHOU X H, LIU S Y, ZHUANG Y, YANG Y. Identification and expression analysis of autophagy-related gene ATG8 family in Solanum melongena L.. Acta Botanica Boreali- Occidentalia Sinica, 2023, 43(10): 1621-1628. (in Chinese)
[36]
GARCIA-MAQUILON I, COEGO A, LOZANO-JUSTE J, MESSERER M, DE OLLAS C, JULIAN J, RUIZ-PARTIDA R, PIZZIO G, BELDA-PALAZÓN B, GOMEZ-CADENAS A, MAYER K F X, GEIGER D, ALQURAISHI S A, ALREFAEI A F, ACHE P, HEDRICH R, PYL8 ABA receptors of Phoenix dactylifera play a crucial role in response to abiotic stress and are stabilized by ABA. Journal of Experimental Botany, 2021, 72(2): 757-774.
[37]
XIA J X, CHEN C, LIU T H, LIU C H, LIU S Y, FANG J G, SHANGGUAN L F. Germplasm resource evaluation and the underlying regulatory mechanisms of the differential copper stress tolerance among Vitis species. Environmental and Experimental Botany, 2023, 206: 105198.
[38]
WANG G L, REN X Q, LIU J X, YANG F, WANG Y P, XIONG A S. Transcript profiling reveals an important role of cell wall remodeling and hormone signaling under salt stress in garlic. Plant Physiology and Biochemistry, 2019, 135: 87-98.
[1] LI Rui, LIANG Yue, BAI Yang, ZHANG GuiYue, WANG NanNan, QIAO SongLin, ZHANG GaiPing. Research Progress on the Roles and Mechanisms of Autophagy Involved in Porcine Reproductive and Respiratory Syndrome Virus Infection [J]. Scientia Agricultura Sinica, 2025, 58(4): 792-801.
[2] ZHANG Ying, YUAN QingYun, REN Fang, HU GuoJun, FAN XuDong, DONG YaFeng. Establishment of RT-qPCR Detection Technology for GINV and Its Spatial and Temporal Distribution in Different Grape Rootstocks [J]. Scientia Agricultura Sinica, 2024, 57(14): 2771-2780.
[3] LI MeiXuan, ZHANG XiangKun, WANG Li, QIAO YueLian, SHI XiaoXin, DU GuoQiang. The Variation of GRSPaV in Different Parts of Shine Muscat Grapevines During Their Phenological Periods [J]. Scientia Agricultura Sinica, 2023, 56(21): 4234-4244.
[4] LI WenHui,HE YiJing,JIANG Yao,ZHAO HongYu,PENG Lei,LI Jia,RUI Rong,JU ShiQiang. Effects of FB1 on Apoptosis and Autophagy of Porcine Oocytes in vitro Maturation [J]. Scientia Agricultura Sinica, 2022, 55(6): 1241-1252.
[5] Xin ZHANG,KongLin HUO,XingXing SONG,DuoNi ZHANG,Wen HU,ChuanHuo HU,Xun LI. Effects of GnIH on Autophagy and Apoptosis of Porcine Ovarian Granulosa Cells via p38MAPK Signaling Pathway [J]. Scientia Agricultura Sinica, 2020, 53(9): 1904-1912.
[6] JIA ShanShan,LUO QiangWei,LI ShaSha,WANG YueJin. Optimization of Embryo Rescue Technique and Production of Potential Seedless Grape Germplasm with Rosy Aroma [J]. Scientia Agricultura Sinica, 2020, 53(16): 3344-3355.
[7] SUN Hong,JIANG YiWen,YU Xin,XIANG GuangQing,YAO YuXin. Effects of Local Root Zone Salinity on Grapevine Injury, Na + Accumulation and Allocation of Carbon and Nitrogen [J]. Scientia Agricultura Sinica, 2019, 52(7): 1173-1182.
[8] SHI XiangBin,WANG XiaoDi,WANG BaoLiang,WANG ZhiQiang,JI XiaoHao,WANG XiaoLong,LIU FengZhi,WANG HaiBo. Requirement Characteristics of Mineral Elements in Different Developmental Phases of Kyoho Grapevine [J]. Scientia Agricultura Sinica, 2019, 52(15): 2686-2694.
[9] BIAN FengE, XIAO QiuHong, HAO GuiMei, SUN YongJiang, LU WenLi, DU YuanPeng, ZHAI Heng. Effect of Root-Applied Melatonin on Endogenous Melatonin and Chlorophyll Fluorescence Characteristics in Grapevine Under NaCl Stress [J]. Scientia Agricultura Sinica, 2018, 51(5): 952-963.
[10] ZHA Qian, XI XiaoJun, JIANG AiLi, TIAN YiHua. Influence of Heat Stress on the Expression of Related Genes and Proteins in Grapevines [J]. Scientia Agricultura Sinica, 2017, 50(9): 1674-1683.
[11] YU Yi-he, LI Xiu-zhen, GUO Da-long, YANG Ying-jun, LI Xue-qiang, ZHANG Guo-hai. Screening and Identification of the Interacting Protein of Cytokinin Response Regulator VvRR2 in Grapevine [J]. Scientia Agricultura Sinica, 2016, 49(6): 1097-1105.
[12] YU Yi-he, LI Xiu-zhen, GUO Da-long, ZHANG Hui-ling, YANG Ying-jun, LI Xue-qiang, ZHANG Guo-hai. Characteristics and Expression of Calmodulin Like B Subunit Interaction Protein VvCIPK10 in Grapevine [J]. Scientia Agricultura Sinica, 2016, 49(19): 3798-3806.
[13] LIU Qing, LUAN Xue-tao, XU Shi-yan,MENG Ying, GAO Jiang-man, XI Zhu-mei. Effect of 24-epibrassinolide Treatment on Grapevine Leaf Against Plasmopara viticola [J]. Scientia Agricultura Sinica, 2016, 49(15): 3010-3018.
[14] ZHANG Yong-jiang, XIN Yan-yan, LI Gui-fen, QIAN Yi-ke. Development of a RT-LAMP Assay for Detection of Grapevine virus A [J]. Scientia Agricultura Sinica, 2016, 49(1): 103-109.
[15] SUN Hong, ZHANG Wei, WEI Xiao-Jing, WANG Hua-Zhong. Identification of Wheat Key Autophagy-Related Factor ATG18 and Profiling of Their Expression Under Biotic and Abiotic Stresses [J]. Scientia Agricultura Sinica, 2014, 47(9): 1657-1669.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd