Scientia Agricultura Sinica ›› 2021, Vol. 54 ›› Issue (8): 1579-1589.doi: 10.3864/j.issn.0578-1752.2021.08.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Polymorphism Analysis Among Chromosomes of Dasypyrum villosum 6V#2 and 6V#4 and Wheat 6A and 6D Based on Wheat SNP Chip

XU ZhiYing1,2(),WANG BaiCui2,MA XiaoLan2,JIA ZiMiao2,YE XingGuo2,LIN ZhiShan2(),HU HanQiao1()   

  1. 1College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, Guangdong
    2Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081
  • Received:2020-08-24 Accepted:2020-10-28 Online:2021-04-16 Published:2021-04-25
  • Contact: ZhiShan LIN,HanQiao HU E-mail:13414916566@163.com;linzhishan@caas.cn;huhanqiao@sina.com

RichHTML

72

PDF

625

Abstract:

【Objective】 The polymorphism among the chromosomes of Dasypyrum villosum 6V#2 and 6V#4 and their wheat homeologous 6A and 6D in the DNA level was compared in this study based on big data to provide a theoretical basis for precision designing breeding of wheat-Dasypyrom villosum targeted chromosome translocation.【Method】A 6V#4(6D) alien disomic substitution line RW15 was crossed with a 6V#2(6A) alien disomic substitution line Nan 87-88, and their F2 plants were detected using 6V#4S/6V#2S/6AS/6DS/6VL specific molecular markers, and the new types of substitution lines were confirmed in F3 employing the above-mentioned markers again as well as genomic in situ hybridization (GISH) technique. Subsequently, polymorphism among the targeted chromosomes in the new types of the substitution lines and their parents Nan 87-88 and RW15 were detected by the probes of 6A and 6D-specific in the wheat 55K chip, and combined with the results of SNP analysis of two accessions of D. villosum with 6A and 6D probes in 660K chip to screen 6V specific SNPs. 【Result】 GISH analysis showed that 19EL124 and 19EL134 had 42 chromosomes including two complete foreign chromosomes in the somatic cells of root tips. Identification using molecular markers showed that 19EL124 had 6V#4S/6DS and missed 6V#2S/6AS specifically amplified bands, while 19EL134 displayed 6V#2S/6AS distinctive bands and lacked 6V#4S-specific bands; both 19EL124 and 19EL134 contained 6VL specific amplified bands, which confirmed that 19EL124 is a 6V#4(6A) disomic substitution line, and 19EL134 is a 6V#2(6D) disomic substitution line. The results of 55K chip showed that the detected efficiency of the key chromosome probes in the alien substitution lines was significantly lower than that of other chromosome probes, and the detected efficiency of different lines in the same type of substitution was also different. Among the 1 177 probes of 6A, the 6A substitution line Nan87-88 and 19EL124 could not be genotyped by 63.21% and 68.90% of the probes, respectively. And the polymorphism between 6V#2 and 6V#4 was detected by 22.51% of the probes. Among the 479 probes of 6D, 49.48% of the probes could not identify the 6D substitution line RW15, and 53.44% could not genotype the 6D substitution line 19EL134. The polymorphism between 6V#2 and 6V#4 was indicated by 16.70% of the 6D probes, there were 23 and 42 probes that could only detect 6V#2 and 6V#4 chromosome, respectively. Twenty-two and fifteen 6V specific SNPs were screened from 395 6A and 231 6D probes through integrating the same genotypes detected by probes which were shared by 55K and 660K chips in this study, and three of them showed polymorphism between 6V#2 and 6V#4 chromosomes. 【Conclusion】 The detection efficiency of the wheat chromosome probes was greatly reduced when the target wheat chromosome was replaced by the alien chromosome, and the proportion of NA genotyping was greatly increased, and most of the NA genotypes showed polymorphism between the two different alien chromosomes in the same type of substitution. The detection efficiency of probe for two alien chromosomes in the same type of alien substitution lines was different, wheat 6A probe could detect 6V#2 better, and wheat 6D probe could detect 6V#4 better. Thirty-seven SNP markers of 6V specific were obtained base on the association analysis of 6V genotyping between D. villosum and the alien substitution lines.

Key words: wheat, Dasypyrum villosum, alien disomic substitution, wheat SNP chip, polymorphism

Table 1

PCR amplification reaction system"

组分 Component 用量 Dosage (μL)
2×Taq master mix 7.5
dd H2O 5.9
正向引物 Forward primer (10 μmol·L-1) 0.3
反向引物 Reverse primer (10 μmol·L-1) 0.3
DNA (100 ng·μL-1) 1.0
总计Total 15.0

Table 2

Information on the molecular markers used in PCR amplification"

引物名称
Primer name		 正向引物
Forward primer (5'-3')		 反向引物
Reverse primer (5'-3')		 检测类型
Types detected		 参考文献
References
6VS-06 GACAGGCAGCTATGAGGC AATCGTCGTTTGGAGTGG 6V#4S [20]
6VS-09 GTAAGAACAAGAGGCTAAACAG CCAGATGACGGTTATTACATAG 6V#4S [20]
6VS-10 GCCATAAGTGACGCTGAT GCATCCTGTGAAGTTGTTG 6V#4S [20]
6VS-12 TGTTGCCTCTCCTCATCA ATTGCTGTCCGCTCATAC 6V#2S
6V#4S		 [20]
6VS-15 AGGACCATACATTCACAGAG TTCCATGAGCAGATTAGCA 6V#2S
6V#4S		 [20]
6VS-18 AGCCAGTAAGATTCCGTATG TCTAACCTTCCTCACAACAC 6V#4S [20]
MBH1 GCCATTATAGTCAAGAGTGCACTAGCTGT AGCTCCTCTCGTTCTCCAATGCT 6V#2S
6V#4S
6AS;6DS		 [10]
P259-1 CGTGATTCAGGAAATGCGATAC TTGCGCCGCCATGTTAG 6V#4S [8]
P461-5a GCGTCATCCGCGCCCGTCAGGT GAGTGCTAATGATAGATGTG 6V#2S
6V#4S		 [8]
N-P5 GCGACCTGTTAGAATGCTATTACGATTAC ATGCTACTCTACCGATGCTTTGAACC 6AS
6DS		 [12]
6V-1 CCGTGCGACAGAACAGAAGTGA GCAATCAGCCACATACAGGTCATC 6VL [20]

Fig. 1

GISH patterns of somatic chromosomes in root tips of alien substitution lines Wheat chromosomes were stained with propidium iodide (PI) in red, and alien chromosomes in yellow-green, which were indicated by the arrows. a-d: RW15, Nan 87-88, 19EL124, 19EL134, 2n=42"

Fig. 2

Amplification patterns of alien substitution lines by molecular markers M: DL2000 marker; 1-7: 19EL124, 19EL134, RW15, Nan87-88, Wan7107, Pm97033, and 10SR3109"

Fig. 3

The SNPs between Nan87-88 and RW15 detected by 6A and 6D-specific probes A: 6A probe; B: 6D probes. a: Total probes and their distribution on the chromosomes; b: Base substitution-type SNP; c: Missing-type SNP(RW15-); d: Missing-type SNP (Nan87-88-)"

Table 3

The NA number of alien substitution lines detected by all probes"

探针数量
No. of probes		 RW15 南87-88
Nan87-88		 19EL124 19EL134
1A（1797） 20 10 51 51
1B（1808） 20 10 22 26
1D（1124） 10 6 8 6
2A（1597） 11 8 9 13
2B（1686） 27 14 27 35
2D（1034） 4 3 10 11
3A（1108） 4 7 11 12
3B（1804） 13 12 16 17
3D（945） 11 6 8 7
4A（1368） 8 11 8 5
4B（1334） 11 6 10 26
4D（477） 6 1 6 6
5A（1791） 25 9 28 6
5B（1637） 18 9 21 14
5D（870） 8 6 19 9
6A（1177） 29 744 811 30
6B（1628） 22 26 21 32
6D（479） 237 13 33 256
7A（1628） 9 13 30 15
7B（1601） 9 13 12 20
7D（1090） 7 4 16 10

Fig. 4

The NA proportion of each chromosome of four alien substitution lines detected by 28020 probes of 55K chip with physically located on wheat chromosomes"

Fig. 5

Differential genotyping between the two alien chromosomes 6V#2 and 6V#4 in the same type of substitution lines detected by 6A and 6D-specific probes, respectively A: Differential genotyping between two 6V(6A) substitution lines of 19EL124 and Nan 87-88 detected by 6A probes. a: Distribution of total differential probes; b: Distribution of differential probes with base substitution; c: Distribution of differential probes with missing-type (19EL124 -); d: Distribution of differential probes with missing-type (Nan 87-88 -). B: Differential genotyping between two 6V(6A) substitution lines of 19EL134 and RW15 detected by 6D probes. a: Distribution of total differential probes; b: Distribution of differential probes with base substitution; c: Distribution of differential probes with missing-type (19EL134 -); d: Distribution of differential probes with missing-type (RW15 -)"

Table 4

SNP between two accessions of Dasypyrum villosum CMM and No.1026 detected by 6A/6B/6D probes of 660K chip"

染色体
Chr.		 探针数量
No. of probes		 总SNP(占比)
Total SNP (percentage)		 碱基替换型SNP(占比)
SNP of base replacement
(percentage)		 CMM-NA SNP(占比)
CMM-NA SNP
(percentage)		 No.1026-NASNP(占比)
No.1026-NA SNP (percentage)
6A 4710 1710(36.31%) 888(18.85%) 414(8.79%) 408(8.66%)
6B 14352 5626(39.20%) 3037(21.16%) 1376(9.59%) 1213(8.45%)
6D 3051 1206(39.53%) 725(23.76%) 247(8.10%) 234(7.67%)
[1] GREADZIELEWSKA A. The genus Dasypyrum-part 2. Dasypyrum villosum-a wild species used in wheat improvement. Euphytica, 2006,152(3):441-454.
[2] MINELLI S, CECCARELLI M, MARIANI M, DE P C, CIONINI P G. Cytogenetics of Triticum×Dasypyrum hybrids and derived lines. Cytogenetic and Genome Research, 2005,109(1/3):385-392.
[3] ZHANG Q P, LI Q, WANG X Y, WANG H Y, LANG S P, WANG Y N, WANG S L, CHEN P D, LIU D J. Development and characterization of a Triticum aestivum-Haynaldia villosa translocation line T4VS·4DL conferring resistance to wheat spindle streak mosaic virus. Euphytica, 2005,145(3):317-320.
[4] ZHANG R Q, FAN Y L, KONG L N, WANG Z J, WU J Z, XING L P, CAO A Z, FENG Y G. Pm62, an adult-plant powdery mildew resistance gene introgressed from Dasypyrum villosum chromosome arm 2VL into wheat. Theoretical and Applied Genetics, 2018,131(12):2613-1620.
doi: 10.1007/s00122-018-3176-5 pmid: 30167758
[5] DE P C, VACCINO P, CIONINI P G, PASQQUINI M, BIZZARRI M, QUALSET C O. Wild Crop Relatives: Genomic and Breeding Resources: Cereals. Heidelberg: Springer, 2011: 185-292.
[6] ZHANG R Q, ZHANG M Y, WANG X E, CHEN P D. Introduction of chromosome segment carrying the seed storage protein genes from chromosome 1V of Dasypyrum villosum showed positive effect on bread-making quality of common wheat. Theoretical and Applied Genetics, 2014,127(3):523-533.
[7] LI H J, CONNER R L, CHEN Q, JIA X, LI H, GRAF R J, LAROCHE A, KUZYK A D. Different reactions to the wheat curl mite and Wheat streak mosaic virus in various wheat Haynaldia villosa 6V and 6VS lines. Plant Disease, 2002,86(4):423-428.
doi: 10.1094/PDIS.2002.86.4.423 pmid: 30818719
[8] 刘畅, 李仕金, 王珂, 叶兴国, 林志珊. 簇毛麦6VS特异转录序列P21461及P33259的获得及其分子标记在鉴定小麦-簇毛麦抗白粉病育种材料中的应用. 作物学报, 2017,43(7):983-992.
LIU C, LI S J, WANG K, YE X G, LIN Z S. Developing of specific transcription sequences P21461 and P33259 on Dasypyrum villosum 6VS and application of molecular markers in identifying wheat-D. villosum breeding materials with powdery mildew resistance. Acta Agronomica Sinica, 2017,43(7):983-992. (in Chinese)
[9] LI S J, WANG J, WANG K Y, CHEN J N, WANG K, DU L P, NI Z F, LIN Z S, YE X G. Development of PCR markers specific to Dasypyrum villosum genome based on transcriptome data and their application in breeding Triticum aestivum-D. villosum#4 alien chromosome lines. BMC Genomics, 2019,20:289.
pmid: 30987602
[10] BIE T D, ZHAO R H, ZHU S Y, CHEN S L, CEN B Q, GAO D R, JIANG Z N, CHEN T T, WANG L, WU R L, HE H G. Development and characterization of marker MBH1 simultaneously tagging genes Pm21 and PmV conferring resistance to powdery mildew in wheat. Molecular Breeding, 2015,35(10):189.
[11] 陈竟男, 马晓兰, 王振, 李仕金, 谢皓, 叶兴国, 林志珊. 基于簇毛麦No.1026转录组的SSR序列分析及其PCR标记开发. 中国农业科学, 2019,52(1):6-15.
12 CHEN J N, MA X L, WANG Z, LI S J, XIE H, YE X G, LIN Z S. ssr sequences and development of pcr markers based on transcriptome of Dasypyrum villosum No.1026. Scientia Agricultura Sinica, 2019,52(1):6-15. (in Chinese)
[12] MA X L, XU Z Y, WANG J, CHEN H Q, YE X G, LIN Z S. Pairing and exchanging between Daypyrum villosum chromosomes 6V#2 and 6V#4 in the hybrids of two different wheat alien substitution lines. International Journal of Molecular Sciences, 2019,20(23):6063.
[13] COLIN R, CAVANAGH, CHAO S M, WANG S C, HUANG B E. Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivars. Proceedings of the National Academy of Sciences of the United States of America, 2013,110(20):8057-8062.
[14] WANG S C, WONG D B, KERRIE F. Characterization of polyploid wheat genomic diversity using the high-density 90,000 SNP array. Plant Biotechnology Journal, 2014,12(6):1-10.
[15] SRBHI G, STELLA H E, YANG C Y, DUNCAN S, STEPHEN A, AMANDA B, PAUL A W, KING L P, KING J L. Detection of T. urartu introgressions in wheat and development of a panel of interspecific introgression lines. Frontiers in Plant Science, 2018,9:1565.
[16] RAZ A, MORAN N, TAMAR E, HANAN S. Ultra-dense genetic map of durum wheat×wild emmer wheat developed using the 90K iSelect SNP genotyping assay. Molecular Breeding, 2014,34(4):1549-1562.
[17] STUART J, LUCAS, AYTEN S, SELAMI Y. High-throughput SNP genotyping of modern and wild emmer wheat for yield and root morphology using a combined association and linkage analysis. Functional and Integrative Genomics, 2017,17(6):667-685.
pmid: 28550605
[18] ZHOU S G, ZHANG J P, CHE Y G, LIU W H, LU Y Q, YSNG X M, LI X Q, JIA J Z, LIU X, LI L H. Construction of Agropyron Gaertn. genetic linkage maps using a wheat 660K SNP array reveals a homoeologous relationship with the wheat genome. Plant Biotechnology Journal, 2018,16(3):818-827.
doi: 10.1111/pbi.12831 pmid: 28921769
[19] 曹廷杰, 谢菁忠, 吴秋红, 陈永兴, 王振忠, 赵虹, 王西成, 詹克慧, 徐如强, 王际睿, 罗明成, 刘志勇. 河南省近年审定小麦品种基于系谱和SNP标记的遗传多样性分析. 作物学报, 2015,41(2):197-206.
CAO T J, XIE J Z, WU Q H, CHEN Y X, WANG Z Z, ZHAO H, WANG X C, ZHAN K H, XU R Q, WANG J R, LUO M C, LIU Z Y. Genetic diversity of registered wheat varieties in henan province based on pedigree and single-nucleotide polymorphism. Acta Agronomica Sinica, 2015,41(2):197-206. (in Chinese)
[20] LI S J, LIN Z S, LIU C, WANG K, DU L P, YE X G. Development and comparative genomic mapping of Dasypyrum villosum 6V#4S- specific PCR markers using transcriptome data. Theoretical and Applied Genetics, 2017,130(10):2057-2068.
pmid: 28653149
[21] WEI W H, QIN R, SONG Y C, NING S B, GUO L Q, GU M G. Genomic in situ hybridization analysis for identification of introgressed segments in alloplasmic lines from Zea mays × Zea diploperennis. Hereditas, 2003,138(1):21-26.
doi: 10.1034/j.1601-5223.2003.01544.x pmid: 12830981
[22] 陈佩度, 周波, 齐莉莉, 刘大钧. 用分子原位杂交(GISH)鉴定小麦-簇毛麦双倍体、附加系、代换系和易位系. 遗传学报, 1995,22(5):380-386.
CHEN P D, ZHOU B, QI L L, LIU D J. Identification of wheat- Haynaldia villosa amphiploid, addition, substitution and translocation lines by in situ hybridization using biotin-labelled genomic DNA as a probe. Acta Genetica Sinica, 1995,22(5):380-386. (in Chinese)
[23] MUKAI Y, GILL B S. Detection of barley chromatin added to wheat by genomic in situ hybridization. Genome, 1991,34(3):448-452.
[24] FRIEBE B, JIANG J, GILL B S, DYCK P L. Radiation-induced nonhomoeologous wheat-Agropyron intermedium chromosomal translocations conferring resistance to leaf rust. Theoretical and Applied Genetics, 1993,86(2/3):141-149.
[25] 赵万春. 普通小麦-簇毛麦整臂互补易位系T1DS·1AL和T1DL·1VS的创制、鉴定和性状评估[D]. 杨凌: 西北农林科技大学, 2010.
ZHAO W C. Development, identification and characterization of two new Triticum aestivum-Dasypyrum villosum conpensation robersonian translocation lines T1DS·1AL and T1DL·1VS[D]. Yangling: Northwest A&F University, 2010. (in Chinese)
[26] BAI S S, YUAN F P, ZHANG H B, ZHANG Z Y, ZHAO J X, YANG Q H, WU J, CHEN X H. Characterization of the Wheat- Psathyrostachys huashania Keng 2Ns/2D substitution line H139: A novel germplasm with enhanced resistance to wheat take-all. Frontiers in Plant Science, 2020,11:233.
pmid: 32210998
[27] LAUREN B, SURBHI G, YANG C Y, STELLA H E, DUNCAN S, STEPHEN A, AMANDA J, ALEXANDRA M, PRZEWIESLIK A, PAUL A, KING L P, KING J L. Exploiting the genome of Thinopyrum elongatum to expand the gene pool of hexaploid wheat. Theoretical and Applied Genetics, 2020,133(7):2213-2226.
doi: 10.1007/s00122-020-03591-3 pmid: 32313991
[28] QI L L, PUMPHRAY M O, FRIEBE B, ZHANG P, QIAN C, BOWDEN R L, ROUSE M N, JIN Y, GILL B S. A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat. Theoretical and Applied Genetics, 2011,123(1):159-167.
pmid: 21437597
[29] LIU C, QI L L, LIU W X, ZHAO W C, JAMIE W, BERND F, BIKRAM S G. Development of a set of compensating Triticum aestivum -Dasypyrum villosum robertsonian translocation lines. Genome, 2011,54(10):836-844.
[30] ZHANG R H, LIU B L, JIANG Z N, CHEN T T, WANG L, JI Y Y, HU Z B, HE H G, BIE T D. Comparative analysis of genetic effects of wheat-Dasypyrum villosum translocations T6V#2S·6AL and T6V#4S·6DL. Frontiers in Plant Science, 2019,138:503-512.
[1] ZHU Qi, JIA ZhenPeng, Tahir SHAH, XU ChenSheng, LI ZhiQi, LÜ HuiShuai, ZHU PengChao, WEI XiaoMin, HUANG DongLin, SUN YanNi, CAO WeiDong, GAO YaJun, WANG ZhaoHui, ZHANG DaBin. Green Manure Crops Combined with Enhanced-Efficiency Products Reduced Greenhouse Gas Emissions and Carbon Footprints in Dryland Wheat Fields [J]. Scientia Agricultura Sinica, 2026, 59(7): 1507-1522.
[2] LI WenHu, LI HaiFeng, DU YuPeng, DING YuLan, LUO YiNuo, LI YuKe, SHE WenTing, ZHANG Feng, TENG Yu, ZHANG SiQi, HUANG Cui, LI XiaoHan, LIU JinShan, WANG ZhaoHui. Regional Differences in Wheat Zinc Uptake and Translocation Responses to Soil Zinc Fertilization [J]. Scientia Agricultura Sinica, 2026, 59(5): 1034-1047.
[3] JIAO WenJuan, HE WanLong, GENG HongWei, BAI Bin, LI JianFeng, CHENG YuKun. Stripe Rust Resistance Evaluation and Molecular Characterization of Yr Genes for 155 Spring Wheat Varieties (Lines) [J]. Scientia Agricultura Sinica, 2026, 59(5): 937-950.
[4] CUI ShiYou, CHEN PengJun, MIAO YuanQing, HAN JiJun, SHEN JunMing. Development and Field Evaluation of Glyphosate-Resistant Wheat Germplasm Generated Through EMS Mutagenesis [J]. Scientia Agricultura Sinica, 2026, 59(4): 723-733.
[5] QIAN Jin, LI YingXue, WU Fang, ZOU XiaoChen. Improved Leaf Phosphorus Content Estimation of Winter Wheat Using Ensemble Hyperspectral Dimensionality Reduction Method [J]. Scientia Agricultura Sinica, 2026, 59(4): 781-792.
[6] KONG Yuan, CUI ShaSha, LI Mei, LI Jian, YANG SiYu, FANG Feng, LIU ShuaiShuai, LIU MingPing, ZENG Yan, GAO XingXiang, BAI LianYang. Spatiotemporal Distribution Dynamics of Five Grass Weed Species Including Lolium multiflorum in Winter Wheat Fields of the Huang- Huai-Hai Region [J]. Scientia Agricultura Sinica, 2026, 59(4): 807-823.
[7] WANG YongSheng, NIU Li, WANG ChangJie, MA LiHua, LIAN XiaoXiao, MENG YaXiong, MA XiaoLe, YAO LiRong, ZHANG Hong, YANG Ke, LI BaoChun, WANG HuaJun, SI ErJing, WANG JunCheng. Genome-Wide Association Study and Candidate Gene Identification for Thousand Grain Weight in Winter Wheat [J]. Scientia Agricultura Sinica, 2026, 59(3): 499-514.
[8] LI XinYi, LI JiaNing, YANG WenPing, XIA Qing, HUO YingRui, HAO ShiHang, HUANG TingMiao, REN YongKang, CHEN Jie, GAO ZhiQiang, YANG ZhenPing. Effects of Post-Anthesis Foliar Zinc Application on Zinc Nutrition in Colored-Grain Wheat [J]. Scientia Agricultura Sinica, 2026, 59(3): 515-527.
[9] XIAN QingLin, XIAO JianKe, GAO AQing, GAO LiChuang, LIU Yang. Effects of Planting Patterns Combined with Soil Moisture Measurement and Supplementary Irrigation on the Yield and Water Use Efficiency of Winter Wheat [J]. Scientia Agricultura Sinica, 2026, 59(3): 589-601.
[10] ZHANG ZhiYong, TAN ShiChao, XIONG ShuPing, MA XinMing, WEI YiHao, WANG XiaoChun. Effects of Annual Water and Nitrogen Optimization on Yield and Nitrogen Migration of Wheat-Maize Rotation System in Irrigation Area of Northern Henan [J]. Scientia Agricultura Sinica, 2026, 59(2): 336-353.
[11] LÜ XuDong, SUN ShiYuan, LI YaNan, LIU YuLong, WANG YanQun, FU Xin, ZHANG JiaYing, NING Peng, PENG ZhengPing. Effects of Intelligent Mechanized Layered Fertilization on Root-Soil Nutrient Distribution and Yield in Wheat Fields [J]. Scientia Agricultura Sinica, 2026, 59(1): 129-146.
[12] LU Hao, ZHANG MingLong, HAN Mei, YAN QingBiao, LI ZhengPeng, YIN Wen, FAN ZhiLong, HU FaLong, CHAI Qiang. Green Manure Returning via Sheep Digest with Nitrogen Fertilizer Reduction are Beneficial to Improve Wheat Yield and Soil Quality at Qinghai-Tibet Plateau [J]. Scientia Agricultura Sinica, 2026, 59(1): 147-160.
[13] YE MeiJin, CHEN JiaTing, ZHOU JieGuang, YIN Li, HU XinRong, LAN YuXin, CHEN Bin, SU LongXing, LIU JiaJun, LIU TianChao, LI XiaoYu, MA Jian. Identification, Validation and Genetic Effect Analysis of Major QTL for Spike Density in Wheat [J]. Scientia Agricultura Sinica, 2026, 59(1): 17-28.
[14] PU LiXia, ZHANG JiaRui, YE JianPing, HUANG XiuLan, FAN GaoQiong, YANG HongKun. The Combined Effects of 16, 17-Dihydro Gibberellin A5 and Straw Mulching on Tillering and Grain Yield of Dryland Wheat [J]. Scientia Agricultura Sinica, 2025, 58(9): 1735-1748.
[15] LI YunLi, DIAO DengChao, LIU YaRui, SUN YuChen, MENG XiangYu, WU ChenFang, WANG Yu, WU JianHui, LI ChunLian, ZENG QingDong, HAN DeJun, ZHENG WeiJun. Genome-Wide Association Study of Heat Tolerance at Seedling Stage in A Wheat Natural Population [J]. Scientia Agricultura Sinica, 2025, 58(9): 1663-1683.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd