Scientia Agricultura Sinica ›› 2020, Vol. 53 ›› Issue (24): 4969-4981.doi: 10.3864/j.issn.0578-1752.2020.24.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genomic Profiling and Expression Analysis of Phosphatidylinositol- specific PLC Gene Families Among Chinese Spring Wheat

SI XuYang1(),JIA XiaoWei1(),ZHANG HongYan1,JIA YangYang1,TIAN ShiJun1,ZHANG Ke2(),PAN YanYun1()   

  1. 1College of Life Sciences, Hebei Agricultural University/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding 071000, Hebei
    2College of Agronomy, Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory of Crop Growth Regulation of Hebei Province, Baoding 071001, Hebei
  • Received:2020-03-23 Accepted:2020-06-02 Online:2020-12-16 Published:2020-12-28
  • Contact: Ke ZHANG,YanYun PAN E-mail:sixuyanglove@163.com;jia999666@126.com;zhangke0126@163.com;pyycell@163.com

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Abstract:

【Objective】To characterize the gene family encoding phosphatidylinositol-specific phospholipase-C (PI-PLC), the gene number, structure, phylogenetic relationship and expression pattern of TaPLC genes were analyzed. In addition, we explored their relative transcription levels in various tissue and the expression pattern under drought and salt stress to further analysis the physiological role of TaPLC genes in response to abiotic stress.【Method】Based on the genome database (Ensembl Plants, http://plants.ensembl.org/index.html), TaPLC genes were identified from Triticum aestivum, and the gene structures were analyzed by some bioinformatics tools, such as Pfam, CDD and SMART. The protein sequence characteristics of the members in TaPLC family were analyzed with the online server ExPASy (http://cn.expasy.org/tools). The subcellular localization of TaPLC genes were predicted using the WoLF PSORT (https://www.genscript.com/wolf-psort.html). Phylogenic tree analysis was performed by software MEGA 7.0. The real-time PCR was used to detect the gene expression levels in different tissues under abiotic stress. 【Result】A total of 11 TaPLC genes members within the heterologous hexaploid wheat genome were identified and analyzed. All TaPLC genes have conserved X-Y catalytic domains and C2 domains, just like other plant PLC genes. Subcellular localization showed that these proteins were located in the chloroplast or mitochondria. Phylogenetic analysis revealed that all of the TaPLC genes were unevenly classified into four numbered, TaPLC1A/TaPLC1D, TaPLC2A/TaPLC2B/TaPLC2D, TaPLC3A/TaPLC3B/TaPLC3D, and TaPLC4A/TaPLC4B/TaPLC4D, which were all orthologous genes of plant PLC genes in plants, and highly conserved during evolution. However, the deletion of TaPLC1 in B subgenome indicates asymmetry evolution in different subgenomes. Further analysis of cis-regulatory elements elucidated that these members of TaPLC family shared similar elements, such as the phytohormone and the stress response cis-elements. Each PLC gene has a unique tissue expression pattern, and they are expressed mainly in roots, leaves, spikes, and gains. In addition, PLC genes mediate the response of wheat to salt and drought stresses, which are induced rapidly by salt or drought stress stimulation. 【Conclusion】The TaPLC family in Triticum aestivum contains 11 members which have conserved X-Y catalytic domains and C2 domains. Although the TaPLC genes were highly conserved, they were asymmetrically evolved in different subgenomes. They had specific expression pattern in different tissues, and were induced by salt or drought stress, which suggested TaPLC genes play a role in response to salt or drought in wheat.

Key words: Triticum aestivum, PI-PLC, salt stress, drought stress

Table 1

Comparative analysis of TaPLC gene families in wheat"

基因名称
Gene name		 基因ID
Gene ID		 染色体定位a
Chromosomal location		 开放阅读框
Open reading frame (bp)		 蛋白质b Protein
大小 Size (aa) 分子量 MW (kD) 等电点 pI
TaPLC1A TraesCS1A02G069300 1A:51700021—51707185 1758 585 66.01 6.07
TaPLC1D TraesCS1D02G071800 1D:52338417—52345805 1761 586 66.19 6.03
TaPLC2A TraesCS2A02G084000 2A:38534860—38538480 1830 609 68.5 5.91
TaPLC2B TraesCS2B02G098500 2B:58321242—58325230 1821 606 68.22 6.06
TaPLC2D TraesCS2D02G082000 2D:35259471—35263395 1824 607 68.45 5.88
TaPLC3A TraesCS4A02G109000 4A:129086595—129090166 1902 633 71.11 5.54
TaPLC3B TraesCS4B02G195200 4B:420197892—420201623 1902 633 71.00 5.75
TaPLC3D TraesCS4D02G195800 4D:340085748—340090648 1902 633 71.05 5.84
TaPLC4A TraesCS5A02G155300 5A:333407514—333413175 1773 590 65.73 6.05
TaPLC4B TraesCS5B02G153600 5B:283008744—283014326 1770 589 65.65 6.06
TaPLC4D TraesCS5D02G160300 5D:250061407—250066699 1770 589 65.65 6.06

Table 2

Subcellular localization of TaPLC protein"

基因Gene 亚细胞定位Subcellular localization
TaPLC1A 线粒体：8,叶绿体：4,细胞核：1 Mito: 8, chlo: 4, nucl: 1
TaPLC1D 线粒体：8,叶绿体：4,细胞核：1 Mito: 8, chlo: 4, nucl: 1
TaPLC2A 叶绿体：7,细胞核：3,线粒体：2.5,线粒体基质：2 Chlo: 7, nucl: 3, mito: 2.5, cyto_mito: 2
TaPLC2B 叶绿体：6,线粒体：5.5,线粒体基质：3.5,细胞核：1 Chlo: 6, mito: 5.5, cyto_mito: 3.5, nucl: 1
TaPLC2D 叶绿体：7,线粒体：4.5,线粒体基质：3,细胞核：1 Chlo: 7, mito: 4.5, cyto_mito: 3, nucl: 1
TaPLC3A 细胞质：8,细胞核：4,叶绿体：1 Cyto: 8, nucl: 4, chlo: 1
TaPLC3B 细胞质：8,细胞核：3,叶绿体：1,线粒体：1 Cyto: 8, nucl: 3, chlo: 1, mito: 1
TaPLC3D 细胞质：6,细胞核：3,线粒体：2,过氧化物：2 Cyto: 6, nucl: 3, mito: 2, pero: 2
TaPLC4A 线粒体：10,叶绿体和线粒体：6.83333,线粒体基质：5.83333,叶绿体：2.5 Mito: 10, chlo_mito: 6.83333, cyto_mito: 5.83333, chlo: 2.5
TaPLC4B 线粒体：10,叶绿体和线粒体：6.83333,线粒体基质：5.83333,叶绿体：2.5 Mito: 10, chlo_mito: 6.83333, cyto_mito: 5.83333, chlo: 2.5
TaPLC4D 线粒体：10,叶绿体和线粒体：6.83333,线粒体基质：5.83333,叶绿体：2.5 Mito: 10, chlo_mito: 6.83333, cyto_mito: 5.83333, chlo: 2.5

Fig. 1

Functional domain analysis of wheat TaPLC protein Numbers indicating positions of each domain in protein sequences"

Fig. 2

Phylogenetic analyses of PLC gene family proteins ☆: Dicots; ★: Monocots; ○: Conifer; ●: Lycophytes; □: Bryphytes; ■: Algae; △: Yeast; ▲: Mammals. Ta: Triticum aestivum; At: Arabidopsis thaliana; Gm: Glycine max; Os: Oryza sativa; Pp: Physcomitrella patens; Ps: Picea sitchensis; N: Nicotiana tabacum; Sl: Solanum licopersicum; Ld: Lilium davidii; Sb: Sorghum bicolor; Pi: Petunia integrifolia; St: Solanum tuberosum; Bn: Brassica napus; Sm: Selaginella moellendorffii; Mp: Micromonas pusilla; Ot: Ostreococcus tauri; Hs: Homo sapiens; Sp: Schizosaccharomyces pombe; Sc: Saccharomyces cerevisiae; Zm: Zea mays"

Fig. 3

Phylogenetic relationship, gene structure and motifs of the TaPLC genes A: The phylogenetic tree of TaPLC genes; B: Schematic diagram for the exon-intron organization of TaPLC基因; C: Schematic diagram for the motifs of TaPLC genes. The motif's sequence and logo diagrams are in S3 Table and S2 Fig."

Fig. 4

Putative regulatory cis-elements in the TaPLC genes promoters of wheat A: Abscisic acid (ABA)-responsive element; E: Ethylene-responsive element; G: Gibberellin-responsive element; P: Gibberellin-responsive element; B: Element involved in the MeJA-responsiveness; D and U: Auxin-responsive element; W: Wound-responsive element; L: Element involved in low-temperature responsiveness; S: MYB binding site involved in drought-inducibility; M: MYB binding site involved in light responsiveness; H: Heat shock-responsive element; R: Element involved in light responsiveness; O: Part of a light responsive element; X: WRKY protein-specific binding site, responsive to pathogen infection. The hormone response cis-elements are red letters; The stress response cis-elements are black letters"

Fig. 5

Tissue expression analysis of TaPLC gene A: RNA-seq data analysis results; B: qRT-PCR results. Lowercase letter indicates significance level (P=0.05) of each gene at different tissues"

Fig. 6

Analysis of TaPLC gene expression under salt and drought condition A: Analysis of TaPLC gene expression under drought condition; B: Analysis of TaPLC gene expression under salt condition. Asterisk indicate the significant difference (P = 0.05) compared with control"

Fig. 7

Analysis of TaPLC genes expression in drought and salt tolerance varieties under drought and salt condition A: Analysis of TaPLC genes expression in drought tolerance varieties under drought condition; B: Analysis of TaPLCs gene expression in salt tolerance varieties under salt condition. KD: Kenong 199; LD: Luohan 7#; SS: Shimai 15#; XS: Xiaoyan 60#"

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