Scientia Agricultura Sinica ›› 2019, Vol. 52 ›› Issue (7): 1164-1172.doi: 10.3864/j.issn.0578-1752.2019.07.004

Special Issue: SPECIAL FOCUS ON GRAPE RESEARCH

• SPECIAL FOCUS ON GRAPE RESEARCH • Previous Articles     Next Articles

The Effects of Different Training Systems and Shoot Spacing on the Fruit Quality of ‘Kyoho’ Grape

JI XiaoHao,LIU FengZhi,SHI XiangBin,WANG BaoLiang,LIU PeiPei,WANG HaiBo()   

  1. Fruit Research Institute, Chinese Academy of Agricultural Sciences/Ministry of Agriculture Key Laboratory of Germplasm Resources Utilization of Horticultural Crops/Key Laboratory of Mineral Nutrition and Efficient Fertilization for Deciduous Fruits, Xingcheng 125100, Liaoning
  • Received:2018-08-01 Accepted:2018-10-31 Online:2019-04-01 Published:2019-04-04
  • Contact: HaiBo WANG E-mail:haibo8316@163.com

Abstract:

【Objective】The aim of this study was to ascertain the effects of different training systems and shoot spacing on the fruit quality of Kyoho grape and provide the theoretical guidance for the canopy management of Kyoho grape. 【Method】 The experimental materials were six-year-old vines of Kyoho grape using Beta grape as rootstock. Two different training systems, including horizontal shoot positioning and Y-shaped training system, were applied. The distance between the shoots was 10 cm, 15 cm and 20 cm controlled by the rope, respectively. Fruit quality indexes, such as berry weight, soluble solids, titratable acid, vitamin C, anthocyanin and aroma, were determined at maturity stage. 【Result】 Berry weight, soluble solids, titratable acid and vitamin C contents were significantly correlated with the training systems, but not significantly correlated with the shoot spacing. The anthocyanin content was significantly correlated with training systems and shoot spacing. The total aroma content was significantly correlated with the training systems, but not significantly correlated with the shoot spacing. Among them, the content of aldehydes, alcohols and terpenes was consistent with the total aroma content, while the content of esters was only correlated with the shoot spacing. The Topsis comprehensive evaluation analysis was conducted for 9 indicators including berry weight, soluble solids, titratable acid, vitamin C, anthocyanin, ester, aldehyde, terpene and alcohol contents, and the result showed that horizontal shoot positioning training system combining with 15 cm shoot spacing scored the highest. 【Conclusion】 These data strongly suggested that horizontal shoot positioning training system combining with 15 cm shoot spacing was the most suitable for Kyoho grape production.

Key words: trainings system, shoot spacing, grape, fruit quality

Table 1

Effect of training system and shoot spacing on Kyoho berry parameters at harvest"

V-10 V-15 V-20 L-10 L-15 L-20
单粒重 Berry weight (g) 9.42±0.49ab 8.24±0.19a 9.42±0.36ab 9.74±0.22b 9.57±0.21b 10.57±0.81b
可溶性固形物含量 Soluble solid content (%) 16.8±0.36a 16.6±0.18a 16.3±0.46a 17.9±0.51b 17.6±0.40b 17.6±0.53b
可滴定酸含量 Titrable acidity (%) 0.55±0.03ab 0.54±0.02ab 0.60±0.06b 0.55±0.03ab 0.50±0.02a 0.52±0.03a
维生素C含量 Vitamin C content (mg·kg-1) 24.99±0.11bc 27.44±0.05d 23.20±0.10b 26.12±0.05cd 24.82±0.10a 21.09±0.03a
花青苷含量 Anthocyanin content (mg·g-1) 2.45±0.24ab 3.80±1.10bc 5.16±1.10c 1.47±0.35a 1.67±0.29a 2.00±0.14ab

Table 2

Correlation analysis between different treatments and fruit quality"

架式
Training system
新梢间距
Shoot spacing
单粒重
Berry weight
0.546* -0.053
可溶性固形物含量
Soluble solid content
0.712** 0.026
可滴定酸含量
Titrable acidity
-0.658** 0.662
维生素C含量
Vitamin C content
-0.525* -0.369
花青苷含量
Anthocyanin content
-0.867** 0.843**

Table 3

Effects of training system and shoot spacing on Kyoho berry volatile aroma"

保留时间Retention time (min) 组分含量 Constituent content (ng·g-1 FW) 组分名称
Aroma component
V-10 V-15 V-20 L-10 L-15 L-20
2.215 1344.41±294.13a 1174.55±113.31a 1287.89±86.25a 1042.11±47.52a 1387.36±154.17a 1236.74±63.24a 乙酸乙酯
Ethyl acetate
5.229 543.26±88.27c 411.65±75.78bc 416.43±44.61bc 175.45±36.34a 200.82±23.16a 277.23±54.1ab 丁酸乙酯
Ethyl butyrate
6.826 53.84±11.83c 40.13±11.72bc 47.78±10.32c 11.81±3.35a 39.8±2.28bc 23.3±2.56ab 2-丁烯酸乙酯
Ethyl 2-butenoate
7.056 839.51±60.72d 723.92±92.77cd 517.88±31.86bc 212.56±63.38a 140.9±6.32a 329.62±137.45ab 2-己烯醛
2-Hexenal
7.791 127.42±13.14cd 138.79±24.8d 120.28±22.61cd 47.27±10.97a 72.29±3.9ab 93.12±5.75bc 甲酸己酯
Hexyl formate
9.342 9.17±3.99abc 6.7±0.69ab 12.69±1.54c 4.71±1.57a 11.34±1.26bc 8±0.93abc 戊酸乙酯
Ethyl valerate
10.694 7.35±1.64ab 9.53±3.66b 5.59±1.41ab 3.93±1.36a 3.96±0.92a 3.65±0.78a α-蒎烯
α-Pinene
11.161 6.75±3.19ab 5.91±3.14ab 6.6±3.02ab 0.67±0.42a 9.13±3.56b 1.79±1.24ab 3-羟基丁酸乙酯
Ethyl 3-hydroxybutyrate
11.351 13.63±3.77c 5.67±1.22ab 8.75±0.74b 2.79±0.22a 6.83±0.6ab 4.11±0.5a 惕各酸乙酯
Ethyl phthalate
12.339 7.95±1.71b 7.48±0.76b 8.57±0.59b 4.53±0.46a 4.35±0.45a 4.3±0.32a 苯甲醛
Benzaldehyde
13.075 3.56±0.38c 5.59±0.47d 3.06±0.09bc 2.6±0.28ab 2.02±0.2a 1.98±0.09a β-蒎烯
β-Pinene
13.724 3.56±1.38b 6.23±0.47c 5.22±0.66bc 1.52±0.08a 1.73±0.2a 1.57±0.06a 1-辛烯-3-醇
1-Octene-3-ol
14.341 12.18±1.85b 15.21±0.96c 12.94±1.13bc 10.36±1.15b 4.49±0.16a 11.63±0.11b 己酸
Caproic acid
14.72 4.62±0.91c 5.17±0.99c 2.59±0.25b 0.39±0.21a 1.4±0.19ab 1.4±0.03ab 2,4-庚二烯醛
2,4-Heptadienal
14.989 162.43±18bc 103.28±25.18ab 240.46±16.12d 92.47±39.22a 197.58±8.88ab 170.57±24.69c 己酸乙酯
Ethyl hexanoate
15.986 6.06±2.11cd 4.98±1.08bc 3.99±0.44bc 0.68±0.1a 8.12±0.48d 2.4±0.11ab 乙酸己酯
Hexyl acetate
16.12 11.89±2.08d 10.46±0.63cd 7.08±0.77bc 3.54±0.59a 7.6±1.81bc 5.4±0.58ab 2-己烯-1-醇乙酸酯
2-Hexen-1-ol acetate
16.413 7.8±2.97b 8.59±2.17b 3.64±2.26a 2.32±0.57a 3.39±0.86a 2.69±0.04a 柠檬烯
Limonene
16.635 0.87±0.05b 1±0.03c 1.28±0.23c 0.45±0.01a 1.17±0.26c 0.53±0.09a 桉油精
Eucalyptol
16.99 1.74±0.15bc 3.38±0.61d 2.51±0.38cd 1.34±0.05ab 3.13±0.53d 0.7±0.09a 苯甲醇
Benzyl alcohol
17.473 18.19±7.43bc 26.77±7.81c 11.39±1.11ab 10.68±1.53ab 4.29±0.08a 6.48±0.68ab 苯乙醛
Phenylacetaldehyde
17.86 17.01±2.9bc 15.92±5.17bc 25.11±2.27c 9.93±6.4a 13.56±0.47ab 13.46±2.62ab 2-己烯酸乙酯
Ethyl 2-hexenoate
19.474 1.46±0.33c 1.49±0.1c 1.64±0.12c 0.81±0.1ab 0.99±0.06b 0.52±0.11c 2-辛烯-1-醇
2-Octene-1-ol
21.183 2.74±0.59c 2.36±0.53bc 2.91±0.42c 1.08±0.3a 2.32±0.05bc 1.33±0.12ab 2,4-己二烯酸乙酯
Ethyl 2,4-hexadienoate
21.523 9.65±1.64bc 6.36±2.48ab 7.54±0.19ab 3.91±1.31a 12.34±0.82c 5.01±0.85a 庚酸乙酯
Ethyl heptanoate
保留时间Retention time (min) 组分含量 Constituent content (ng·g-1 FW) 组分名称
Aroma component
V-10 V-15 V-20 L-10 L-15 L-20
21.776 5.53±0.59ab 8.94±0.57c 6.64±0.57b 5.99±0.45ab 5.32±0.06a 5.94±0.42ab 壬醛
Nonanal
22.211 18.54±1.28a 45.98±6.11b 42.62±5.16b 17.7±2.59a 9.83±1.31a 15.67±2.31a 苯乙醇
Phenylethanol
23.888 3.91±0.66c 4.5±0.33c 4.16±0.39c 2.33±0.3b 1.21±0.35a 2.16±0.13ab 苄基腈
Benzyl nitrile
26.379 2.72±0.19b 3.01±0.5bc 3.75±0.46c 1.7±0.36a 2.52±0.29ab 1.71±0.07a 萜品烯
Terpinene
27.32 0.44±0.06bc 0.69±0.12d 0.64±0.02cd 0.16±0.04a 1.63±0.1e 0.28±0.08ab 丁二酸二乙酯
Diethyl succinate
27.471 2.38±0.2b 4.49±0.57c 2.37±0.28b 0.2±0.07a 1.73±0.21b 0.25±0a 水杨酸甲酯
Methyl salicylate
27.629 2.49±1.34bc 1.9±0.26b 2.46±0.3bc 0.44±0.19a 3.76±0.24b 1±0.34a 4-辛烯酸乙酯
Ethyl 4-octenoate
28.246 12.29±4.49ab 16.94±7.36ab 12.36±1.29ab 9.43±3.14a 20.63±1.8b 10.06±3.1ab 辛酸乙酯
Ethyl octanoate
28.665 0.91±0.09a 1.31±0.23ab 1.08±0.09ab 0.92±0.15a 1.46±0.23b 0.9±0.15a 癸醛
Capraldehyde
30.279 2.56±0.83bc 1.83±0.38b 3.25±0.51c 0.28±0.04a 1.26±0.68ab 0.27±0.09a 香茅醇
Citronellol
31.165 18.29±1.55b 17.98±6.24b 17.33±0.6b 5.55±0.84a 5.56±1.08a 7.41±0.47a 苯乙酸乙酯
Phenylacetate
31.497 2.95±1.29b 1.75±0.43ab 2.33±0.31ab 1.03±0.35a 2.39±0.23ab 0.98±0.11a 2-辛烯酸乙酯
Ethyl trans-2-octenoate
34.811 1.45±0.31b 2.33±0.52c 1.37±0.18ab 1.34±0.11ab 2.98±0.31c 0.59±0.06a 壬酸乙酯
Ethyl pelargonate
40.079 0.72±0.22ab 1.18±0.28b 1.03±0.18b 0.34±0.06a 2.15±0.15c 0.39±0.07a 4-癸烯酸乙酯
Ethyl 4-decenoate
41.115 2.95±0.69ab 2.24±0.03a 4.24±0.57b 5.92±0.65c 2.63±0.42a 2.92±0.41ab 癸酸乙酯
Ethyl caprate
45.616 3.09±0.47bc 4.73±0.76cd 5.54±0.55d 1.29±0.32a 4.13±0.93cd 1.57±0.36ab 2,4-癸二烯酸乙酯
Ethyl 2,4-decadienoate

Fig. 1

Analysis of the contents of four aroma substances Different letters in the same group indicate significant differences among treatments at P<0.05"

Table 5

Topsis comprehensive evaluation analysis"

样本
Treatments
D+ D- CI 名次
Ranking
V-10 0.1143 0.0912 0.4437 6
V-15 0.1308 0.1073 0.4506 4
V-20 0.1204 0.0978 0.4483 5
L-10 0.1166 0.1025 0.4679 3
L-15 0.1025 0.1264 0.552 1
L-20 0.1093 0.1245 0.5325 2
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