葡萄果实MYBA1与UFGT、DFR的作用机制

doi:10.3864/j.issn.0578-1752.2018.12.013

Abstract

Abstract: 【Objective】MYBA1 transcription factor plays an important role in the biosynthesis of anthocyanins. To further explore the synergistic regulatory mechanism of anthocyanins, we analyzedthe mutual mechanism and expression pattern of VvMYBA1, VvUFGT, VvDFR and content of anthocyanins during grape berry development.【Method】The real-time PCR, spectrophotometer and SAS8.0 were used to analyze the expression patterns of VvMYBA1, VvUFGT, VvDFR content of anthocyanins and correlation in different time periods, respectively. The transcriptional activity of VvMYBA1 and the interactions between the VvMYBA1 and VvUFGT, VvDFR were determined by yeast hybrid system. 【Result】The expression pattern analysis showed that the expression of VvMYBA1, VvUFGT and VvDFR was increased until veraison (60-80 d after full bloom) and then declined. The content of anthocyanins was fist increased until veraison (80 d after full bloom) and then stabilization. Correlation analysis showed that the expression of MYBA1 with expression ofthe DFR, UFGT had positive correlation, and the content of anthocyanins with the expression of MYBA1, DFR, UFGT had positive correlation. Yeast hybrid assay indicated that VvMYBA1 had transcriptional activation functions which could specifically combine promoter of VvUFGT, VvDFR and had no interaction with VvUFGT and VvDFR encoded proteins.【Conclusion】The content of anthocyanins was significantly correlated with expression of VvMYBA1, VvDFR, and VvUFGT. The VvMYBA1 had transcriptional activation functions. All results indicated that VvMYBA1 could regulate the accumulation of anthocyanins in grape berry development by regulating VvUFGT and VvDFR expression combined with VvUFGT and VvDFR promoter specifically.

Key words: grape, VvMYBA1; VvUFGT, VvDFR, yeast hybrid system

NIU TieQuan, DONG YanMei, LIU HaiXia, ZHANG XiaoJun, GAO Yan, ZHANG PengFei, LIANG ChangMei, WEN PengFei. The Regulations of the MYBA1 in UFGT and DFR from the Grape Berries[J].Scientia Agricultura Sinica, 2018, 51(12): 2368-2377.

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