中国荷斯坦牛CXCR1基因编码区SNP多态与临床乳房炎和生产寿命的关联分析

doi:10.3864/j.issn.0578-1752.2017.12.016

Abstract

Abstract: 【Objective】 The objective of this study was to investigate the association between the SNPs in the coding sequence (CDS) of CXCR1 gene and the clinical mastitis and lifetime for Chinese Holstein.【Method】SNPs in the CDS of CXCR1 gene were screened by using PCR and direct sequencing for 20 cows with low SCS and 20 cows with high SCS. Finally, the selected SNPs of CXCR1 gene for 866 Chinese Holstein cows were detected using flight mass spectrometry. The clinical mastitis and productive life of tested cows were collected from the management system of dairy farm. The association between the SNPs and the clinical mastitis and productive life of tested cows was analyzed using multi factor variance analysis, Logistic regression, Cox regression.【Result】A total of 13 SNPs were found in the CDS region of CXCR1 gene (291 C＞T, 333 C＞G, 337 A＞G, 365 C＞T, 570 A＞G, 642 A＞G, 735 C＞G, 816 A＞C, 819 A＞G, 980 A＞G、, 995 A＞G, 1008 C＞T and 1068 A＞G), and these SNPs were divided into 4 linkage groups, and four SNPs were selected from each linkage group for further analysis（642 A＞G, 816 A＞C, 980A＞G and 1068 A＞G). A total of 9 haplotypes were observed for 4 SNPs of CXCR1 gene, the GAGG haplotype frequency was the highest (0.3141), and the frequency of haplotype ACAA was the lowest (0.0017). CXCR1-642 A＞G showed a significant association with the number of dairy cows suffering from clinical mastitis in second lactation (P＜0.05). The number of dairy cows suffering from clinical mastitis for individual with AG genotype was significantly higher than that of AA genotype (P＜0.05). The number of dairy cows suffering from clinical mastitis for individual with CXCR1-816 AA genotype was significantly higher than that of AC and CC genotypes (P＜0.05). The other SNPs showed no significant association with the number of clinical mastitis. CXCR1-816 A＞C mutation showed an extremely significant association with culling age (P＜0.01) and a significant association with productive month and culling lactation (P＜0.05). The productive month, culling age and culling lactation for the individual of CXCR1-816 AA were significantly higher than that of CC genotype (P＜0.05). Cox regression showed that only CXCR1-816 showed significant association with the productive life（P＜0.05), the survival probability of individual with CC genotype was significantly lower than that of AA and AC genotype in each time period. 【Conclusion】The mutation of CXCR1-816 A＞C showed significant association with the clinical mastitis and lifetime for Chinese Holstein, and this SNP could be used for molecular marker-assisted selection of productive life for Chinese Holstein when the molecular function of this mutation were proved.

Key words: Chinese Holstein, CXCR1, SNPs, clinical mastitis, productive lifetime

WANG MengQi, NI Wei, ZHANG HuiMin, YANG ZhangPing, WANG XiPu, JIANG YanSen, MAO YongJiang. Association Between SNPs in the CDS Regions of CXCR1 Gene and the Clinical Mastitis and Lifetime for Chinese Holstein[J].Scientia Agricultura Sinica, 2017, 50(12): 2359-2370.

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Association Between SNPs in the CDS Regions of CXCR1 Gene and the Clinical Mastitis and Lifetime for Chinese Holstein

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