Scientia Agricultura Sinica ›› 2016, Vol. 49 ›› Issue (18): 3542-3550.doi: 10.3864/j.issn.0578-1752.2016.18.008

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• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Differences in Carbon Accumulation and Transport in Brassica napus with Different Nitrogen Use Efficiency and Its Effects on Oil Formation

ZHANG Xiao-long1, ZHANG Zhen-hua1, SONG Hai-xing1, YU Jia-ling1, GUAN Chun-yun2   

  1. 1College of Resources and Environmental Sciences, Hunan Agricultural University/ Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China/National Engineering Laboratory of Soil and Fertilizer Resources Efficient Utilization/ Hunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resources Use/Hunan Provincial Key Laboratory of plant Nutrition in Common University, Changsha 410128
    2 National Center of Oilseed Crops Improvement, Hunan Branch, Changsha 410128
  • Received:2016-01-25 Online:2016-09-16 Published:2016-09-16

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Abstract: 【Objective】The differences in carbon (C) accumulation and transport in Brassica napus with different nitrogen use efficiencies (NUE), were studied in order to supply a theoretical basis for elucidating the mechanism of high NUE genotypes in coordinating the contradiction between C and N metabolism and promoting oil formation.【Method】A soil culture experiment was conducted to study the differences of C accumulation and distribution in different organs of B. napus with different NUE at different growth stages (stem elongation stage, flowering stage, silique stage and harvest stage) under normal- and limited-N conditions, and using 13C isotope labeling to determine the C redistribution proportion and amount from vegetative organs to reproductive organs, analyze the contribution of C accumulation and transport to grain yield and oil accumulation.【Result】No significant differences in grain oil content between high- and low-NUE genotypes under the normal and limited-N application levels were observed, but the oil yield of high-NUE genotype was significantly higher than that of low-NUE genotype. Compared with limited-N, oil content was slightly decreased, but oil production was increased significantly under normal-N. There was no differences in C accumulation amount between high- and low-NUE genotypes, while the differences in C accumulation amount at different N application levels was significant, C accumulation amount of normal –N was significantly higher than that of limited-N. C allocation proportion in plant organs between high- and low-NUE genotypes was different, C allocation proportion in roots and leaves of high-NUE genotype was higher than that of low-NUE genotype at stem elongation stage and flowering stage, and C allocation proportion in silique and grain of high-NUE genotype was higher than that of low-NUE genotype at silique stage and harvest stage. While, C allocation proportion in stem of high-NUE genotype was lower than that of low-NUE genotype at the whole growth stage, and C allocation proportion in stem and root of high-NUE genotype was lower than that of low-NUE genotype at the whole growth stage. Compared with limited-N, C allocation proportion in leaves was increased at stem elongation stage and flowering stage under normal-N, while C allocation proportion in root was decreased. In addition, C allocation proportion in grain at harvest stage under normal-N was higher than what was observed in limited-N. As the development of plant growth stages, C distribution proportion and amount from vegetative organs to reproductive organs was gradually increased, the differences between genotypes were increased. Redistribution proportion and amount of C from vegetative organs to silique at silique stage and redistribution proportion and amount of C from vegetative organs to grain at harvest stage in high-NUE genotype was higher than that in low-NUE genotype, the significant different was occurred under normal-N. Redistribution proportion and amount of C in silique of high-NUE genotype was lower than that in low-NUE genotype under limited-N, and reversed results was observed under normal-N, the significant difference was occurred under limited-N. The 50% or more of C accumulation at stem elongation stage was left from vegetative organs, which was corresponding with redistribution proportion into reproductive organs, but C reduction proportion was significantly higher than C re-distribution proportion, which was because of the carbohydrate respiration consumption. 【Conclusion】 There was no significant difference in C accumulation between high- and low-NUE B. napus genotypes at different growth stages. However, higher proportion of C was redistributed from vegetative organs to reproductive organs in high-NUE genotype than that in low-NUE genotype at later growth stages. This is one of the important mechanisms for high-NUE genotype possesses with higher C source to relieve the contradictions between C and N, and promote oil formation during the process of grain formation.

Key words: Brassica napus, carbon accumulation, carbon transport, oil formation, nitrogen use efficiency

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