Scientia Agricultura Sinica ›› 2016, Vol. 49 ›› Issue (9): 1746-1756.doi: 10.3864/j.issn.0578-1752.2016.09.011

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• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Domestic and Abroad Research Progress of Potato Tuber-Specific Storage Protein Patatin

ZHANG Du-qin, MU Tai-hua, SUN Hong-nan   

  1. Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Processing, Ministry of Agriculture, Beijing 100193
  • Received:2015-09-16 Online:2016-05-01 Published:2016-05-01

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Abstract: Potatoes are the fourth main crops after wheat, rice, and corn. China is the world’s largest potato production country. Along with the development of the potato processing industry, studies on potatoes are going on gradually. Patatin is the potato tuber-specific storage protein, as well as the main part of the potato protein. In addition to its Lipid acyl hydrolase (LAH) and antioxidant activity, Patatin has excellent physico-chemical and functional properties such as solubility, emulsifying, foaming, and gelling properties, and is one of the most popular vegetable proteins. However, no overviews about the physico-chemical properties, the isolation, extraction and purification methods, the application in the food industry, and the future research hotspots of patatin were found up to now. In this paper, the molecular weight, structural characteristics, and physico-chemical properties of patatin were introduced, the method of isolation, extraction, and purification methods of patatin were summarized, and the advantages and disadvantages of each method were compared in order to provide a theoretical reference for the further application, research, and exploitation of patatin in the food industry. The content of patatin in potato protein ranged from 5.4% to 38.0%, relating to the cultivars and metabolic physiology of potato. The molecular weight of patatin varied from 39 kDa to 45 kDa, depending on the degree of glycosylation. Patatin is a kind of globulin with compact structure, and the primary structure contains more than 360 amino acid residues and remained unaffected by the surrounding elements, while the advanced structures were affected by temperature, pH, and so on. Patatin has excellent physico-chemical and functional properties such as solubility, emulsifying, foaming, and gelling properties, which were affected by surrounding elements. Gel chromatography and ion-exchange chromatography are the most common methods to isolate and purify patatin from potato protein, the reversed-phase chromatography (RPC), immunoaffinity chromatography, and gene expression can produce highly purified patatin. Although not suitable for large-scale production, expanded bed adsorption (EBA) chromatography can prepare large amount of patatin with low purity. Research studies on the application of patatin in the food industry are scarce. Patatin has great potential as food ingredients in the food industry, further research studies on Patatin will continue progress toward simplifying extraction methods, cutting the extraction cost, and developing foods, health products, and drugs with high-functional value.

Key words:  potato, Patatin, food industry, application, research and exploitation

[1]    贾晶霞, 杨德秋, 李建东, 李洋. 中国与世界马铃薯生产概况对比分析与研究. 农业工程, 2011, 1(2): 84-86.
Jia J X, Yang D Q, Li J D, Li Y. Research and comparative analysis about potato production situation between China and continents in the world. Agricultural Engineering, 2011, 1(2): 84-86. (in Chinese)
[2]    潘牧, 彭慧元, 邓宽平, 雷尊国, 李怀奎, 牟时锋. 马铃薯蛋白的研究进展. 贵州农业科学, 2012, 40(10): 22-26.
Pan M, Peng H Y, Deng K P, Lei Z G, Li H K, Mou S F. Advances in potato protein. Guizhou Agricultural Science, 2012, 40(10): 22-26. (in Chinese)
[3]    谢从华. 马铃薯产业的现状与发展. 华中农业大学学报(社会科学版), 2012, 97: 1-4.
Xie C H. The current situation and the development of potato industry. Journal of Huazhong Agricultural University (Social Science), 2012, 97: 1-4. (in Chinese)
[4]    FAOSTAT: Food And Agriculture Organization of The United Nations-STATISTICS DIVISION. (2014-08-04) [2015-09-06]. http:// faostat3.fao.org/faostat-gateway/go/to/browse/Q/QC/E.
[5]    谢庆华, 吴毅歆. 马铃薯品种营养成分分析测定. 云南师范大学学报, 2002, 22(2): 50-52.
Xie Q H, Wu Y Z. Nutrient determination and analysis of Different Potato varieties. Journal of Yunnan Normal University, 2002, 22(2): 50-52. (in Chinese)
[6]    RYAN C A. Quantitative determination of soluble cellular proteins by radial diffusion in agar gels containing antibodies. Analytical Biochemistry, 1967, 19(3): 434-440.
[7]    Wojnowska I, Poznanski S, Bednarski W. Processing of potato protein concentrates and their properties. Journal of Food Science, 1982, 47(1): 167-172.
[8]    Knorr D, Kohler G O, Betschart A A, Potato protein concentrates: the influence of various methods of recovery upon yield, compositional and functional characteristics. Journal of Food Processing and Preservation, 1977, 1(3): 235-247.
[9]    Knorr D. Effect of recovery methods on yield, quality and functional properties of potato protein concentrates. Journal of Food Science, 1980, 45(5): 1183-1186.
[10]   张泽生, 刘素稳, 郭宝芹. 马铃薯蛋白质的营养评价. 食品科技, 2007(11): 219-221.
Zhang Z S, Liu S W, Guo B Q. Nutritional evaluation of potato protein. Food science and technology, 2007(11): 219-221. (in Chinese)
[11]   Koningsveld G. Physico-chemical and functional properties of potato proteins [D]. Wageningen: Wageningen University, 2001.
[12]   Pots A M, Gruppen H, Hessing M, van Boekel M A, Voragen A G. Isolation and characterization of Patatin isoforms. Journal of Agricultural and Food Chemistry, 1999, 47(11): 4587-4592.
[13]   Park W D, Blackwood C, Mignery G A, Hermodson M A, Lister R M. Analysis of the heterogeneity of the 40000 molecular weight tuber glycoprotein of potatoes by immunological methods and by NH2-terminal sequence analysis. Plant Physiology, 1983, 71(1): 156-160.
[14]   Racusen D, Foote M. A major soluble glycoprotein of potato TUBERS1. Journal of Food Biochemistry, 1980, 4(1): 43-52.
[15]   Strætkvern K O, Schwarz J G, Wiesenborn D P, Zafirakos E, Lihme A. Expanded bed adsorption for recovery of Patatin from crude potato juice. Bioseparation, 1998, 7(6): 333-345.
[16]   Sonnewald U, Studer D, Rocha-Sosa M, Willmitzer L. Immunocytochemical localization of Patatin, the major glycoprotein in potato (Solanum tuberosum L.) tubers. Planta, 1989, 178(2): 176-183.
[17]   Sonnewald U, Sturm A, Chrispeels M J, Willmitzer L. Targeting and glycosylation of Patatin the major potato tuber protein in leaves of transgenic tobacco. Planta, 1989, 179(2): 171-180.
[18]   Stiekema W J, Heidekamp F, Dirkse W G, Beckum J, Haan P, Bosch C, Louwerse J D. Molecular cloning and analysis of four potato tuber mRNAs. Plant Molecular Biology, 1988, 11(3): 255-269.
[19]   Segrest J P, Jackson R L. Molecular weight determination of glycoproteins by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. Methods in Enzymology, 1972, 28: 54-63.
[20]   Racusen D, Foote M. A major soluble glycoprotein of potato TUBERS1. Journal of Food Biochemistry, 1980, 4(1): 43-52.
[21]   Ba?rta J, Ba?rtova? V, Zdra?hal Z, S?edo O. Cultivar variability of patatin biochemical characteristics: Table versus processing potatoes (Solanum tuberosum L.). Journal of Agricultural and Food Chemistry, 2012, 60(17): 4369-4378.
[22]   Pouvreau L, Gruppen H, Piersma S R, Van den Broek L A M, Van Koningsveld G A, Voragen A G J. Relative abundance and inhibitory distribution of protease inhibitors in potato juice from cv. Elkana. Journal of Agricultural and Food Chemistry, 2001, 49(6): 2864-2874.
[23]   Bártová V, Bárta J, Brabcová A, Zdráhal Z, Horá?ková V. Amino acid composition and nutritional value of four cultivated South American potato species. Journal of Food Composition and Analysis, 2015, 40: 78-85.
[24]   Waglay A, Karboune S, Alli I. Potato protein isolates: Recovery and characterization of their properties. Food Chemistry, 2014, 142: 373-382.
[25]   Pots A M, Gruppen H, van Diepenbeek R, van der Lee J J, van Boekel M A J S, Wijngaards G, Voragen, A G J. The effect of storage of whole potatoes of three cultivars on the Patatin and protease inhibitor content; a study using capillary electrophoresis and MALDI-TOF mass spectrometry. Journal of the Science of Food and Agriculture, 1999, 79(12): 1557-1564.
[26]   Mignery G A, Pikaard C S, Hannapel D J, Park W D. Isolation and sequence analysis of cDNAs for the major potato tuber protein, Patatin. Nucleic Acids Research, 1984, 12(21): 7987-8000.
[27]   Pikkard C S, Brusca J S, Hannapel D J, Park W D. The two classes of genes for the major potato tuber protein, Patatin, are differentially expressed in tubers and roots. Nucleic Acids Research, 1987, 15(5): 1979-1994.
[28]   Twell D, Ooms G. Structural diversity of the Patatin gene family in potato cv. Desiree. Molecular and General Genetics MGG, 1988, 212(2): 325-336.
[29]   Racusen D, Weller D L. Molecular weight of Patatin, A major potato tuber PROTEIN1. Journal of Food Biochemistry, 1984, 8(2): 103-107.
[30]   Seo S, Karboune S, Archelas A. Production and characterisation of potato Patatin–galactose, galactooligosaccharides, and galactan conjugates of great potential as functional ingredients. Food Chemistry, 2014, 158: 480-489.
[31]   Creusot N, Wierenga P A, Laus M C, Giuseppin M L, Gruppen H. Rheological properties of Patatin gels compared with β-lactoglobulin, ovalbumin, and glycinin. Journal of the Science of Food and Agriculture, 2011, 91(2): 253-261.
[32]   Pots A M, de Jongh H H J, Gruppen H, Hessing M, Voragen A G. The pH dependence of the structural stability of Patatin. Journal of Agricultural and Food Chemistry, 1998, 46(7): 2546-2553.
[33]   Pots A M, de Jongh H H J, Gruppen H, Hamer R J, Voragen A G. Heat-induced conformational changes of Patatin, the major potato tuber protein. European Journal of Biochemistry, 1998, 252(1): 66-72.
[34]   van Koningsveld G A, Gruppen H, de Jongh H H J, Wijngaards G, van Boekel M A J S, Walstra P, Voragen A G J. The solubility of potato proteins from industrial potato fruit juice as influenced by pH and various additives. Journal of the Science of Food and Agriculture, 2002, 82(1): 134-142.
[35]   Pots A M, Gruppen H, Hessing M, van Boekel M A, Voragen A G. Isolation and characterization of Patatin isoforms. Journal of Agricultural and Food Chemistry, 1999, 47(11): 4587-4592.
[36]   阚建全. 食品化学. 2版. 北京: 中国农业大学出版社, 2008: 44-102.
Kan J Q. Food Chemistry. 2nd Ed. Beijing: China Agricultural University Press, 2008: 44-102. (in Chinese)
[37]   van Koningsveld G A, Gruppen H, de Jongh H H J, Wijngaards G, van Boekel M A, Walstra P, Voragen A G. The effects of ethanol on structure and solubility of potato proteins and effects of its presence during the preparation of a protein isolate. Journal of Agricultural and Food Chemistry, 2002, 50: 2947-2956.
[38]   van Koningsveld G A, Gruppen H, de Jongh H H J, Wijngaards G, van Boekel M A, Walstra P, Voragen, A G. Effects of pH and heat treatments on the structure and solubility of potato proteins in different preparations. Journal of Agricultural and Food Chemistry, 2001, 49(10): 4889-4897.
[39]   Fitzsimons S M, Mulvihill D M, Morris E R. Denaturation and aggregation processes in thermal gelation of whey proteins resolved by differential scanning calorimetry. Food Hydrocolloids, 2007, 21(4): 638-644.
[40]   Pots A M, ten Grotenhuis E, Gruppen H, Voragen A G, de Kruif K G. Thermal aggregation of Patatin studied in situ. Journal of Agricultural and Food Chemistry, 1999, 47(11): 4600-4605.
[41]   朴金苗, 都凤华, 齐斌. 马铃薯分离蛋白凝胶的制备及其性质研 究. 食品科学, 2010(22): 108-111.
Piao J M, Du F H, Qi B. Solubility and emulsifying properties of potato protein isolate. Food Science, 2010(22): 108-111. (in Chinese)
[42]   van Koningsveld G A, Walstra P, Gruppen H, Wijngaards G, van Boekel M A, Voragen A G. Formation and stability of foam made with various potato protein preparations. Journal of Agricultural and Food Chemistry, 2002, 50(26): 7651-7659.
[43]   Ralet M C, Guéguen J. Fractionation of potato proteins: solubility, thermal coagulation and emulsifying properties. LWT-Food Science and Technology, 2000, 33(5): 380-387.
[44]   Ralet M C, Guéguen J. Foaming properties of potato raw proteins and isolated fractions. LWT-Food Science and Technology, 2001, 34(4): 266-269.
[45]   Foegeding E A, Davis J P. Food protein functionality: A comprehensive approach. Food Hydrocolloids, 2011, 25(8): 1853-1864.
[46]   Galliard T. The enzymic deacylation of phospholipids and galactolipids in plants. Purification and properties of a lipolytic acyl-hydrolase from potato tubers. Biochemistry Journal, 1971, 121: 379-390.
[47]   Strætkvern K O, Schwarz J G. Recovery of native potato protein comparing expanded bed adsorption and ultrafiltration. Food and Bioprocess Technology, 2012, 5(5): 1939-1949.
[48]   Løkra S, Schüller R B, Egelandsdal B, Engebretsen B, Strætkvern K O. Comparison of composition, enzyme activity and selected functional properties of potato proteins isolated from potato juice with two different expanded bed resins. LWT-Food Science and Technology, 2009, 42(4): 906-913.
[49]   Elias R J, Kellerby S S, Decker E A. Antioxidant activity of proteins and peptides. Critical Reviews in Food Science and Nutrition, 2008, 48(5): 430-441.
[50]   Petersen B, Petersen T N, Andersen P, Nielsen M, Lundegaard C. A generic method for assignment of reliability scores applied to solvent accessibility predictions. BMC Structural Biology, 2009, 9(1): 51.
[51]   Liu Y W, Han C H, Lee M H, Hsu F L, Hou W C. Patatin, the tuber storage protein of potato (Solanum tuberosum L.), exhibits antioxidant activity in vitro. Journal of Agricultural and Food Chemistry, 2003, 51(15): 4389-4393.
[52]   Sun Y, Jiang L, Wei D. Partial characterization, in vitro antioxidant and antiproliferative activities of Patatin purified from potato fruit juice. Food & Function, 2013, 4(10): 1502-1511.
[53]   Cheng Y, Xiong Y L, Chen J. Antioxidant and emulsifying properties of potato protein hydrolysate in soybean oil-in-water emulsions. Food Chemistry, 2010, 120(1): 101-108.
[54]   Miedzianka J, P?ksa A, Anio?owska M. Properties of acetylated potato protein preparations. Food Chemistry, 2012, 133(4): 1283-1291.
[55]   吕建国, 安兴才. 膜技术回收马铃薯淀粉废水中蛋白质的中试研究. 中国食物与营养, 2008(4): 37-40.
LÜ J G, An X C. Pilot study on the membrane technology in recovery of protein from potato starch wastewater. The Food Industry Science and Technology, 2008(4): 37-40. (in Chinese)
[56]   宋艳艳, 孔维宝, 宋昊, 华绍烽, 夏春谷. 磁性壳聚糖微球的研究进展. 化工进展, 2012, 31(2): 345-354.
Song Y Y, Kong W B, Song H, Hua S F, Xia C G. Reseach progress in magnetic chitosan microspheres. Chemical Industry and Engineering Progress,2012 31(2): 345-354. (in Chinese)
[57]   Strætkvern K O, Lokra S, Olander M A, Lihme A. Food-grade protein from industrial potato starch effluent recovered by an expanded bed adsorption process. Journal of Biotechnology, 2005, 118: S33.
[58]   崔竹梅, 黄海珊, 秦欢欢, 朴金苗, 齐斌. 马铃薯蛋白组分的分离提取和功能性质研究. 食品科学, 2011, 32(3): 76-80. (in Chinese)
Cui Z M, Huang H S, Qin H H, Piao J M, Qi B. Isolation and characterization of potato protein fractions. Food Science, 2011, 32(3): 76-80. (in Chinese)
[59]   Seppälä U, Alenius H, Turjanmaa K, Reunala T, Palosuo T, Kalkkinen N. Identification of Patatin as a novel allergen for children with positive skin prick test responses to raw potato. Journal of Allergy and Clinical Immunology, 1999, 103(1): 165-171.
[60]   Rosahl S, Schmidt R, Schell J, Willmitzer L. Isolation and characterization of a gene from Solanum tuberosum encoding Patatin, the major storage protein of potato tubers. Molecular and General Genetics MGG, 1986, 203(2): 214-220.
[61]   Andrews D L, Beames B, Summers M D, Park W D. Characterization of the lipid acyl hydrolase activity of the major potato (Solanum tuberosum) tuber protein, Patatin, by cloning and abundant expression in a baculovirus vector. Biochemistry Journal, 1988, 252: 199-206.
[62]   Wahl R, Lau S, Maasch H J, Wahn U. IgE-mediated allergic reactions to potatoes. International Archives of Allergy and Immunology, 1990, 92(2): 168-174.
[63]   Koppelman S J, van Koningsveld G A, Knulst A C, Gruppen H, Pigmans I G, de Jongh H H. Effect of heat-induced aggregation on the IgE binding of Patatin (Sol t 1) is dominated by other potato proteins. Journal of Agricultural and Food Chemistry, 2002, 50(6): 1562-1568.
[64]   Spelbrink R E J, Lensing H, Egmond M R, Giuseppin M L. Potato Patatin generates short-chain fatty acids from milk fat that contribute to flavour development in cheese ripening. Applied Biochemistry and Biotechnology, 2015, 176(1): 231-243.
[65]   Macrae A R, Visicchio J E, Lanot A. Application of potato lipid acyl hydrolase for the synthesis of monoacylglycerols. Journal of the American Oil Chemists’ Society, 1998, 75(11): 1489-1494.
[66]   Gambuti A, Rinaldi A, Moio L. Use of patatin, a protein extracted from potato, as alternative to animal proteins in fining of red wine. European Food Research and Technology, 2012, 235(4): 753-765.
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