Abstract:

【Objective】The objective of the study was to improve the breeding efficiency of seedless grape through embryo rescue in vitro and investigate the effects of various factors on seedlings in vitro. 【Method】 Hybrid ovules of 7 cross combinations were used to investigate the effect of different genotypes, different ovule culture media, supplementation of different aminophenols, low-temperature treatment on the proportion of seedlings of harvested fruits in vitro. 【Result】 The results indicated that among the investigated embryoes of seedless grape, the female parent and male parent had influence on seedlings. In cross combinations with diploid seedless grapes as female parent, diploid seedless grapes as male parent was suitable for seedlings than tetraploid seeded grapes. Among the three different media, MM4 medium was most suitable for embryo development and seedlings in vitro culture of seedless grapes. ER medium supplemented with 4 mmol?L-1 proline was most suitable for embryo development in vitro culture of seedless grapes. The effect of low-temperature treatment of Ruby Seedless×Centennial Seedless and Ruby Seedless×Black Olympia for 3 days was the best in seedling growth. 【Conclusion】 ER medium supplemented with 4 mmol?L-1 proline or MM4 medium are suitable for embryo rescue in vitro culture of seedless grapes. Low-temperature treatment of fruits for 3 days, has a positive effect on improvement of embryo rescue efficiency of diploid seedless grape×diploid seedless grape.

Key words: seedless grape, embryo rescue, proportion of seedlings