Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (21): 4309-4317.doi: 10.3864/j.issn.0578-1752.2014.21.016

• HORTICULTURE • Previous Articles     Next Articles

Evolutionary Study of MAM Genes Which Function in the Process  of Glucosinolates Synthesis in Brassica rapa

LIU Jin, ZHANG Ji-fang, LIANG Jian-li, CHENG Feng, WU Jian, WANG Xiao-wu   

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crop Biology and Germplasm Creation of The Ministry of Agriculture, Beijing 100081
  • Received:2014-03-20 Revised:2014-04-25 Online:2014-11-01 Published:2014-11-01

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Abstract: 【Objective】The objective of this study is to explore the duplication and evolution of Brassica rapa MAM which are key genes in the process of glucosinolates synthesis. This study will provide a theoretical basis for future MAM research. 【Method】 The synthenic relationship of MAM genes among Arabidopsis thaliana, Schrenkiella parvula and Brassica rapa were analyzed, according to B. rapa datebase BARD, conserved motif of MAM genes were predicted by online software MEME, the expression of MAM homologous genes in 93 materials of Brassica rapa was analyzed by software R, and the phylogenetic tree was constructed by software MEGA 5.05. 【Result】 The results showed that the MAM homologous genes were located in three subgenomes of B. rapa. Amongst seven MAM genes, five geneswhich are synthenic orthologs to A. thaliana mainly originated from whole genome triplication. The remaining two genes may derive from translocation. Motif analysis result indicated that there were many differences in both ends of protein sequences in BrMAM homologous genes, compared with AtMAM genes. Expression data showed that the expression level of these homologous genes was variable, Bra021947 and Bra018524 expressed little in several materials of B. rapa, the expression of Bra029356 could be detected in all materials. In the phylogenetic tree, BrMAM homologous genes and AtMAM genes were located in different branches, in addition to Bra018524. 【Conclusion】 These results indicated that the B. rapa MAM genes had an independent evolution after the divergence of B. rapa and A. thaliana.

Key words: Brassica rapa, glucosinolates, BrMAM genes, synteny, phylogenetic tree

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