枯草芽胞杆菌Bs-916的全基因组分析

doi:10.3864/j.issn.0578-1752.2011.23.006

Abstract

Abstract: 【Objective】The objective of this study is to sequence the whole genome of Bacillus subtils Bs-916 and provide more information about molecular biology for future mining and utilizing the potential of this strain. 【Method】 Application of comparative genomics softwares, the whole genome sequence analysis was carried out with Bs168 strains. 【Result】 Bs-916 strains genome is 3 925 958 base pairs comprises 4 056 protein-coding genes. The average GC ratio is 46.4%, the highest compared with other whole-genome sequencing of Bacillus spp.. It contains 152 tandem repeat region, 103 transposons, 37 IS (Insert sequence), 46 tRNA, 39 rRNA. Through comparative genomics analysis, Bs-916 harbors eight giant gene clusters directing synthesis of bioactive peptides and polyketides by modularly organized mega-enzymes named non-ribosomal peptide synthetases, NRPS and polyketide synthases, PKS. Macrolactin, difficidin, and bacillomycin L are absent in Bs168 strains. Bs-916 also contains phytase gene, comAPQX and sfp related with biocontrol mechanism.【Conclusion】Bs-916 strain genome contains many gene cluster encoding a variety of antimicrobial substances, it can be considered a paradigm for an own group of plant-associated gram-positive bacteria with a huge potential for biocontrol and plant growth promotion. The complete genome sequence along with its amenability to genetic manipulation, should facilitate exploitation of the hitherto unappreciated potential of strain Bs-916 to produce secondary metabolites for developing agrobiological engineering preparations.

Key words: Bacillus subtils, comparative genomics, synteny, GC skew agents with predictable features

WANG Xiao-Yu, LUO Chu-Ping, CHEN Zhi-Yi, LIU Yong-Feng, LIU You-Zhou, NIE Ya-Feng, YU Jun-Jie, YIN Xiao-Le. The Complete Genome Sequence of the Gram-Positive Bacterium Bacillus subtils Bs-916[J].Scientia Agricultura Sinica, 2011, 44(23): 4807-4814.

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The Complete Genome Sequence of the Gram-Positive Bacterium Bacillus subtils Bs-916

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