捕食螨携带白僵菌孢子的能力及所携孢子的活性和毒力

doi:10.3864/j.issn.0578-1752.2014.20.008

Abstract

Abstract: 【Objective】 The objective of this study is to assess the capability of predatory mite carrying conidia and test the vitality of conidia, which will provide a theoretical basis for combining application of Beauveria bassiana with predatory mite against Frankliniella occidentalis. 【Method】 Neoseiulus barkerimL B. bassiana suspensions, the potential of conidia carried by predator was observed under scanning electron microscope. The number of conidia carried by predator at different times (2, 12, 24 and 48 h) was counted under hemocytometer, and the vitality of conidia was tested by colonies counting. Moreover, the infection rates of conidia dispersed by predator at different times above were tested by bioassay. 【Result】The treated predatory mites could carry conidia. After 2 h, one female adult predator carried 4.12×10⁴ conidia. The number of conidia carried was decreased gradually with time increasing, after 48 h, one predator carried 7.95×10³ conidia. The conidia dispersed by predators had vitality. After 2 h, the conidia dispersed by 10 predators formed 24 colonies on medium. The number of colonies was decreased gradually with time increasing, after 48 h, only 3 colonies could be formed. The conidia dispersed by predators were virulent to F. occidentalis. After 2 h, the conidia dispersed by 10 predators caused 75% mortality of adult F. occidentalis on the 10th day. The mortality of F. occidentalis was decreased gradually with time increasing, after 48 h, 25% mortality of adult F. occidentalis was caused. After 2, 12, 24 and 48 h, the mortalities of adult F. occidentalis over the 10 days displayed linear correlation. The lethal time (LT₅₀) after 2 h was 6.44 d, which was significantly lower than that after 48 h (9.83 d). 【Conclusion】Predator mites can carry a large number of conidia within a short time, the conidia have vitality and can be dispersed and infected F. occidentalis, therefore, combined application of B. bassiana and predatory mite have a potential for control of F. occidentalis. adults were sprayed by 1.0×10⁸conidia/

Key words: Neoseiulus barkeri, Beauveria bassiana, conidia, Frankliniella occidentalis

WU Sheng-yong, WANG Peng-xin, ZHANG Zhi-ke, XU Xue-nong, LEI Zhong-ren. Effects of Fertilization on Aggregate Characteristics and Organic Carbon Distribution in a Paddy Soil in Dongting Lake Plain of China[J].Scientia Agricultura Sinica, 2014, 47(20): 3999-4006.

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Effects of Fertilization on Aggregate Characteristics and Organic Carbon Distribution in a Paddy Soil in Dongting Lake Plain of China

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