Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (20): 3992-3998.doi: 10.3864/j.issn.0578-1752.2014.20.007

• PLANT PROTECTION • Previous Articles     Next Articles

Identification and Pathogenicity of Pathogen Casuing Anthracnose on Vaccinium

XU Cheng-nan1,2, WANG Ya-nan1, HU Tong-le1, WANG Shu-tong1, ZHOU Zong-shan2,CAO Ke-qiang1   

  1. 1College of Plant Protection, Agricultural University of Hebei, Baoding 071001, Hebei
    2Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, Liaoning
  • Received:2014-04-14 Revised:2014-05-26 Online:2014-10-16 Published:2014-10-16

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Abstract: 【Objective】The objective of this study is to identify the pathogen causing stem and leaf anthracnose on Vaccinium in Liaoning, and to provide an IPM strategy for disease control and resistance breeding.【Method】Diseased samples were collected from Xingcheng and Zhuanghe County, Liaoning Province and disinfected stem and leaf pieces were placed on acidified potato dextrose agar (PDA) plate. Two represent isolates LNSW1 and B-Cg1 were chosen for morphological and molecular study. Isolates were characterized by colony morphology, conidial characteristics under 28℃ in dark. The internal transcribed spacer (ITS) region of the ribosomal DNA (ITS1-5.8S-ITS2) of isolates LNSW1 and B-Cg1 were amplified and sequenced. The consensus ITS sequences of isolates LNSW1 and B-Cg1 were compared with Colletotrichum spp. associated sequences in GenBank. Mega5.1 was used to draw the phylogenetic tree of isolates LNSW1, B-Cg1 and other related sequences. Another molecular identification of the two isolates were carried out with amplification of species-specific primer pair CaInt(CaInt2)/ITS4. Pathogenicity of both species was verified by mycelium plug inoculation on eight attached Vaccinium cultivars.【Result】Thirty-six ColletotrichumAB729126, KF698729, and EU886755 were divided into one group, and isolate B-Cg1 and CJF923828, KF516931, and GU174547 were divided into another group, the homology of each isolate in two groups was between 99%-100%. Additionally, the species-specific amplification results showed that both primer pairs could clearly distinguish the two different isolates. Pathogenicity tests results showed that the two Colletotrichum【Conclusion】Identification of the pathogen was based on morphology of colony and conidial, pathogenicity tests, nucleotide sequence of rDNA-ITS and species-specific primer pair CaInt(CaInt2)/ITS4. The typical anthracnose symptoms ofVaccinium in Liaoning Province were described in this study, disease diagnosis and pathogen identification was carried out based on the morphological characteristics, molecular technique and pathogenicity tests. Combined these results, it was confirmed that the causal agent of Vaccinium anthracnose in Liaoning were C. acutatum and C. gloeosporioides. spp. could both cause anthracnose lesions on leaves and stems of Vaccinium. The pathogenicity of C. acutatum was a little higher than C. gloeosporioides on Northern Highbush Vaccinium.. gloeosporioides isolates isolates were collected from Liaoning Province, two different groups were divided by morphology. Represent isolates LNSW1 and B-Cg1 were different in morphology of colony and conidia, and were skeptically identified as C. acutatum and C. gloeosporioides. The rDNA-ITS sequences length of isolates LNSW1 and B-Cg1 was 557 and 547 bp. The phylogenetic analyses showed that isolate LNSW1 and C. acutatum isolates

Key words: Vaccinium anthracnose, Colletotrichum acutatum, Colletotrichum gloeosporioides, pathogenicity

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