Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (9): 1858-1864.doi: 10.3864/j.issn.0578-1752.2014.09.021

• RESEARCH NOTES • Previous Articles    

Genetic Polymorphism of the SCD1 Gene and Its Associations with Milking Traits in Chinese Holstein

 WANG  Xiao-Long, CHANG  Ling-Ling, CHEN  Ying, CHEN  Ren-Jin, LI  Yun-Long, MAO  Yong-Jiang, JI  De-Jun, YANG  Zhang-Ping   

  1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu
  • Received:2013-09-05 Online:2014-05-01 Published:2014-03-14

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Abstract: 【Objective】This experiment was conducted to explore the association between SCD1 gene and milking traits in Chinese Holstein in south China. 【Method】 The polymorphism of SCD1 gene was investigated for 323 Chinese Holstein cattle from the experimental farm of Yangzhou University using Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) technique, at the same time, from 140 Chinese Holstein with similar body condition (BCS=3.0±0.5), no clinical mastitis, 2-3 parities and during lactation, 100 mL mixed milk samples a day (morning﹕noon﹕evening=4﹕3﹕3) were collected for determining the content of fatty acid, and then 100 mL mixed milk samples were collected all day (morning﹕mid-day﹕evening), which were used for determining fatty acid, and the effects of alleles on the component of fatty acids were then analyzed using a mixed animal model. 【Result】Two polymorphic sites, SCD1-762 and SCD1-878, were detected with the advantageous allele frequencies of A at SCD1-762 and C at SCD1-878 were 0.7897 and 0.7835, respectively. χ2 test indicated that these two sites were in the Hardy-Weinberg equilibrium. Linkage disequilibrium existed between SCD1-762 and SCD1-878 (r2=0.879) at a very significant level (P＜0.01). Four haplotypes were defined at the two sites, TC, CC, TT, and CT. Effect of SCD1-762, SCD1-878 or certain haplotypes was significant on the ratio of MUFA and the total saturation degree in milk (P＜0.05). The ratio of MUFA and total saturation degree in milk of haplotype TC was significantly higher than CT, while the ratio of SFA was significantly lower than CT (P＜0.05).【Conclusion】The two polymorphic sites had some genetic effects on milking traits, but further researches are needed for its mechanism.

Key words: Chinese Holstein , SCD1 , polymorphism , milking traits

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