Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (21): 4506-4514.doi: 10.3864/j.issn.0578-1752.2013.21.013

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Expression of Soluble Acid Invertase Gene and Its Relationship with Sugar Accumulation in Sweet Sorghum Stem

 NIE  Yuan-Dong-1, ZHONG  Hai-Li-1, DUN Bao-Qing-1 , YE  Kai-2, WANG  Zhi-1, ZHU  Li-3, LI  Gui-Ying-1   

  1. 1.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/Biomass Energy Research Center/National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing 100081
    2.Institute of Biomass Energy, Xinjiang Academy of Agricultural Sciences, Urumuqi 830091
    3.Institute of Biotechnology, Chinese Academy of Agricultural Sciences, Beijing 100081
  • Received:2013-05-15 Online:2013-11-01 Published:2013-05-15

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Abstract: 【Objective】To better understand the mechanism of sucrose accumulation in sweet sorghum stem, the correlation between sugar content and soluble acid invertase(SAI) gene expression was analyzed by monitoring the expression of SAI in leaves and stems and the sugar content in stems.【Method】The sugar content and expression of SAI gene was investigated by using high performance liquid chromatography (HPLC) and qRT-PCR respectively.【Result】Reducing sugar was the major component at the elongation stage, and sucrose level was lower, then sucrose content was increased significantly at heading and flowering stages, until reached the maximum level at milking and ripening stages, while it declined after ripening. The expression of SAI gene in high-sugar varieties was higher than mid, low, and pith dry varieties in leaves, while it was just the reverse in stems. The sugar content of sweet sorghum at ripening stage was significantly negatively correlated with the expression of SAI gene in stems (r=-0.5971), and was significantly positively correlated with the expression of SAI gene in leaves (r=0.7239).【Conclusion】Sucrose accumulation was correlated with the expression of SAI gene in leaves and stems: a significantly positive correlation between sucrose and the expression of SAI gene in leaves, while a significant negative correlation between sucrose and the expression of SAI gene in stems, the correlation coefficient with leaves was higher than with stem.

Key words: sweet sorghum , sugar accumulation , soluble acid invertase (SAI) gene , expression analysis

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