Abstract:

【Objective】 The present study aims to explore potentially superior sources of fertility-restoration and possible distribution for 1BL/1RS male sterile lines of Aegilops cytoplasm and to provide a guarantee for practical application of “three lines system” of strong vigor in wheat hybrid breeding and production via identifying 1BL/1RS translocation and categorizing restoring degrees of fertility among a vast number of wheat varieties (lines) randomly selected world-widely including different ecological areas in China and Canada. 【Method】 Combining molecular and cytological techniques, the selected 256 wheat varieties (lines), as well as 8 male sterile lines, were tested for 1BL/1RS translocation, and the degrees of fertility-restoration were evaluated by Chinese domestic methods. 【Result】 Approximately 20 % of the tested 256 wheat varieties (lines) were characterized with 1BL/1RS translocation, and the non-1BL/1RS wheat varieties (lines) were grouped into semi-sterility (about 86.15%), high-fertility (91.67%) and compete-fertility (100%); and the 1BL/1RS wheat varieties (lines) were mainly grouped into high-sterility and complete sterility accounting for 40%, respectively. The potential sources of fertility-restoration for male sterile lines were Chinese spring wheat region (60%) and South winter wheat areas including the middle and low Yangtze valley winter wheat region (65.85%), Southwestern winter wheat region (68.42%) and South of China winter wheat region (71.43%).【Conclusion】 The results indicate a potential basis of non-1BL/1RS wheat varieties (lines) for superior restoration of fertility for 1BL/1RS male sterile lines of Aegilops cytoplasm in Chinese spring wheat region and South winter wheat areas.

Key words: 1BL/1RS wheat, molecular cytology, male sterile lines with Aegilops cytoplasm, fertility-restoring, location