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    15 July 2010, Volume 43 Issue 14
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
    Relationship Between QTLs for Plant Height and Its Components and Genes Controlling Gibberellin and Brassinosteroid Biosynthesis and Their Transduction in Japonica Rice (Oryza sativa L.)
    JIANG Shu-kun,HUANG Cheng,XU Zheng-jin,CHEN Wen-fu
    Scientia Agricultura Sinica. 2010, 43(14):  2829-2838 .  doi:10.3864/j.issn.0578-1752.2010.14.001
    Abstract ( 815 )   PDF (517KB) ( 1246 )   Save
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    【Objective】 To understand and provide a theoretical basis for the molecular mechanism of plant height in rice, QTLs controlling plant height and its component factors were detected and compared with the genes involved in gibberellin and brassinosteroid biosynthesis and transduction. 【Method】 The QTLs affecting plant height and its component factors were analyzed by employing 126 recombinant inbred lines (RILs) derived from a cross between two japonica rice cultivars, Shennong 265 and Lijiangxintuanheigu, and then compared them with the genes involved in gibberellin and brassinosteroid biosynthesis and transduction. 【Result】 Plant height and its component factors showed a continuous normal distribution in the RILs population. The plant height showed a high positive correlation with its component factors. The correlation between adjacent plant height components was positively significant while the significance of the correlation between non-adjacent plant height components was less or even negative. Further result indicated that the plant height was mainly affected by the length of I internode and IV internode from top. A total of 21 QTLs controlling plant height and its components were identified on chromosomes 1, 2, 3, 5, 6, 7, 8, 9, 11 and 12, respectively. QPH9b (EP1, DEP1 or qPE9-1) on chromosome 9 plays a very important role in affecting plant height through controlling the I internode and II internode length from top. Its molecular function was different from the other genes controlling plant height identified previously. So, it would provide a novel mechanism for plant height. 【Conclusion】 Comparison between 21 QTLs and genes controlling gibberellin and brassinosteroid biosynthesis and transduction indicated that the genetic basis of plant height is extremely complex in this RILs population.

    Molecular and Cytological Analyses of 1BL/1RS Wheat and Fertility-Restoring Locations for Male Sterile Lines with Aegilops Cytoplasm
    GUO Yan-ping,WEI Fang,ZHANG Gai-sheng,CHENG Hai-gang,SONG Yu-long,WANG Qing,NIU Na,MA Shou-cai,LI Hong-xia
    Scientia Agricultura Sinica. 2010, 43(14):  2839-2847 .  doi:10.3864/j.issn.0578-1752.2010.14.002
    Abstract ( 1020 )   PDF (494KB) ( 571 )   Save
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    【Objective】 The present study aims to explore potentially superior sources of fertility-restoration and possible distribution for 1BL/1RS male sterile lines of Aegilops cytoplasm and to provide a guarantee for practical application of “three lines system” of strong vigor in wheat hybrid breeding and production via identifying 1BL/1RS translocation and categorizing restoring degrees of fertility among a vast number of wheat varieties (lines) randomly selected world-widely including different ecological areas in China and Canada. 【Method】 Combining molecular and cytological techniques, the selected 256 wheat varieties (lines), as well as 8 male sterile lines, were tested for 1BL/1RS translocation, and the degrees of fertility-restoration were evaluated by Chinese domestic methods. 【Result】 Approximately 20 % of the tested 256 wheat varieties (lines) were characterized with 1BL/1RS translocation, and the non-1BL/1RS wheat varieties (lines) were grouped into semi-sterility (about 86.15%), high-fertility (91.67%) and compete-fertility (100%); and the 1BL/1RS wheat varieties (lines) were mainly grouped into high-sterility and complete sterility accounting for 40%, respectively. The potential sources of fertility-restoration for male sterile lines were Chinese spring wheat region (60%) and South winter wheat areas including the middle and low Yangtze valley winter wheat region (65.85%), Southwestern winter wheat region (68.42%) and South of China winter wheat region (71.43%).【Conclusion】 The results indicate a potential basis of non-1BL/1RS wheat varieties (lines) for superior restoration of fertility for 1BL/1RS male sterile lines of Aegilops cytoplasm in Chinese spring wheat region and South winter wheat areas.

    Analysis of Genetic Diversity in ICRISAT Mini Core Collection of Peanut (Arachis hypogaea L.) by SSR Markers
    REN Xiao-ping,ZHANG Xiao-jie,LIAO Bo-shou,LEI Yong,HUANG Jia-quan,CHEN Yu-ning,JIANG Hui-fang
    Scientia Agricultura Sinica. 2010, 43(14):  2848-2858 .  doi:10.3864/j.issn.0578-1752.2010.14.003
    Abstract ( 818 )   PDF (303KB) ( 742 )   Save
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    【Objective】 Assessing the genetic diversity of peanut mini core collection in ICRISAT and analyzing their genetic relationships among various groups of taxonomy, and verifying the fitness of traditionally botanical taxonomic system under cultivated peanut were carried out in the study, which could provide essential information for the exploration and utilization of ICRISAT peanut mini core collection. 【Method】 One hundred and sixty-eight peanut accessions from 42 counties of five continents were employed for SSR analysis using 27 polymorphic primer pairs in this study. Three-dimensional PCA graphs was conducted and drawn in NTSYS-pc V 2.0 statistical package. Parameters of genetic diversity (Nei78 genetic distances, et al.) were proceeded in Popgene V1.32 statistical package. The dendrogram of UPGMA method were drawn in MEGA3.1 statistical package. 【Result】 One hundred and fifteen polymorphic bands were amplified using 27 SSR primer pairs with unambiguous unique polymorphic bands. In average, 4.2930 alleles were observed, 65.49% of which was effective alleles (2.7931) in each SSR primer pair. PM137, 16C6, 14H6, 8D9 and 7G02 were the most effective SSR pairs, I value of which was over 1.5, alleles of which was over 5, effective alleles of which was over 3.7. SSR alleles were uniformly distributed among botanical taxon units in cultivated peanut. For ssp. fastigiata var. fastigiata, genetic diversity of germplasm from North America and India were lower, and that from South America showed higher. For ssp. fastigiata var. vulgaris, genetic diversity of germplasm from North America and Europe was lower, and that from South America and Africa was higher. For ssp. hypogaea var. hypogaea, genetic diversity of germplasm from North America was lower, and that from South America and Africa and USA was higher. So, genetic diversity of peanut germplasm from South America was abundant, which conformed that peanut originates in South America. Four taxonomic clusters were detected in ICRISAT mini core peanut collection by using PCA analysis. Gene pool “hypogaea” mainly consisted of var. hypogaea, gene pool “vulgaris” mainly consisted of var. vulgaris, while gene pool “fastigiata 1” and “fastigiata 2”mainly consisted of var. fastigiata. Nei’78 genetic distance among botanical taxon based groups of peanut genetic resources ranged from 16.336-23.607 cM. Five large cluster groups were identified based on the UPGMA dendrogram. Group 1 equals to “vulgaris” gene pool; Group 3 equals to “fastigiata 1”and “fastigiata 2” gene pool; and Group 4 equals to “hypogaea” gene pools; Group 2 and 5 stands for var. peruviana and var. aequatoriana respectively. The UPGMA clustering results generally support the PCA clustering results. 【Conclusion】 There were significant differences among most botanical groups in ICRISAT mini core collection, with clear separation of four gene pools for genetic diversity structure. The research results partially support the traditional botanical taxonomy under A. hypogaea L. In order to broaden the genetic bases of peanut breeding, the genetic potentials in the ICRISAT mini core collection should be thoroughly exploited.
    TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY
    Root Characteristics of High-Yield C Liangyou Rice Combinations of Two-Line Hybrid Rice
    TANG Wen-bang,DENG Hua-bing,XIAO Ying-hui,ZHANG Gui-lian,FAN Ke,MO Hui,CHEN Li-yun
    Scientia Agricultura Sinica. 2010, 43(14):  2859-2868 .  doi:10.3864/j.issn.0578-1752.2010.14.004
    Abstract ( 1032 )   PDF (267KB) ( 757 )   Save
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    【Objective】 The root characteristics of C Liangyou rice combinations and the correlation between root characteristics and yield were studied. 【Method】 The root morphology, root physiology as well as the correlation between root characteristics and yield were studied by using seven C Liangyou rice combinations as experimental materials and Shanyou 63 as a control. 【Result】 The results showed that root dry weight, number of adventitious root , total length of adventitious root, total root volume, root growth abilities, total root absorption area,root active absorption area and root oxidation ability of the combination were significantly higher than those of the control Shanyou 63. Root dry weight, number of adventitious root , total length of adventitious root, total root absorption area,and root oxidation ability at the heading stage, and root growth abilities at the grain filling stage and the milking stage had a significant positive correlation with yield per plant. Stepwise regression indicated that the number of adventitious root and root oxidation ability at the heading stage were the main influencing factors of high yield of rice and could be regarded as reliable indicators for cultivation and management and genetic inprovement of rice. 【Conclusion】 It is concluded that root morphology is good and the root is vigorous in C Liangyou rice combinations at all stages, its morphological and physiological characteristics ensure the efficient absorption of nutrient and water in the whole growing period, which is the base leading to high yield of C Liangyou rice combinations. Strong root vigor at the late growing stage, and proper ratio of root to shoot and the harmonious growth of root and shoot are also the main causes leading to high yield of C Liangyou rice combinations.

    Effects of Allocations of Row-Spacing on Photosynthetic Characteristics and Yield Structure of Winter Wheat Cultivars with Different Spike Types
    LI Na-na,LI Hui,PEI Yan-ting,SHI Yü-hua,TIAN Qi-zhuo,XIE Lian-jie,WANG Shu-liang,LIU Xin,XU Feng-jiao
    Scientia Agricultura Sinica. 2010, 43(14):  2869-2878 .  doi:10.3864/j.issn.0578-1752.2010.14.005
    Abstract ( 884 )   PDF (348KB) ( 1109 )   Save
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    【Objective】 The objectives of this study were to investigate the influences of row-spacing on photosynthetic characteristics and yield structure of two winter wheat cultivars with different spikes types to provide a theoretical basis for super-high-yield wheat cultivation. 【Method】 Field experiments were conducted from 2006 to 2008 to study the regulatory effect of five different allocations of row-spacing on grain yield components, photosynthesis and senescence characteristics using two different wheat cultivars including JM 20, a multiple-spike cultivar and SN 8355, a large-spike cultivar. Ten-treatment combinations were arranged in a completely randomized block design with three replicates. 【Result】 The results showed that the equal and narrow row-spacing increased the canopy apparent photosynthesis (CAP) and leaf area index (LAI) from spike-forming to 7 days after anthesis, and then the relationship reversed. Wide spacing form had higher flag leaves photosynthetic rate (Pn) than other treatments, and the increased range was gradually getting biger over time. The antioxidative enzymes activity had a similar trend with Pn in different treatments of row-spacing, but had an opposite trend with MDA. SN 8355 delayed senescencing and the photosynthetic rate was better than JM 20 at the later period of grain-filling. The correlation between photosynthetic rate and grain yield was not significant, and it showed a great difference between CAP and yield components at different growth stages. Pn and CAP had a significant positive correlation from 14 days after anthesis. 【Conclusion】 The row-spacing could adjust yield components and finally affect the grain yield. Wide row-spacing could improve the photosynthesis characteristics and delay the senescence. Different varieties have to match with different row-spacing allocations to reach their maximal yields.

    Recent Progresses in Monitoring Crop Spatial Patterns by Using Remote Sensing Technologies
    TANG Hua-jun,WU Wen-bin,YANG Peng,ZHOU Qing-bo,CHEN Zhong-xin
    Scientia Agricultura Sinica. 2010, 43(14):  2879-2888 .  doi:10.3864/j.issn.0578-1752.2010.14.006
    Abstract ( 1060 )   PDF (275KB) ( 1482 )   Save
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    As a new high-technology with an advantage of high temporal resolution, wide coverage and low cost, remote sensing is currently used in a wide arrange of earth observation activities and thus provides a useful tool to detect and monitor the spatial patterns of crop cultivation. Based on the systematic summary of the progress of studies in remote-sensing-based monitoring of spatial patterns of agricultural crops in the latest decade, including its theories, methods and applications, a series of problems that should be urgently resolved in the study are put forward, and some important study directions and priorities for future are viewed. Studies show that crop acreage can be monitored according to the differences in spectral characteristics of different crops, which are normally recorded by the satellite sensors. There are three major approaches used for crop acreage monitoring: spectral-based identification, phenology-based identification and multiple data-fusion-based identification methods. Mapping multiple cropping systems using remote sensing is mainly based on the crop growth curves, which can be derived from the smoothed time-series vegetation index (VI) data. Furthermore, cropping patterns can be also examined through discriminating the crop growth period from variations in time-series VI data and characteristics of different cropping patterns. How to construct the theoretical and technological systems, to develop and verify the image classification methods, to optimize the smoothing methods for time-series data and to improve the capability of automatic extraction of information could be the major development trends of this field in the future.

    PLANT PROTECTION
    Evaluation of Resistance of Current Wheat Cultivars to Stripe Rust in Northwest China, North China and the Middle and Lower Reaches of Changjiang River Epidemic Area
    HAN De-jun,WANG Qi-lin,ZHANG Li,WEI Guo-rong,ZENG Qing-dong,ZHAO Jie,WANG Xiao-jie,HUANG Li-li,KANG Zhen-sheng
    Scientia Agricultura Sinica. 2010, 43(14):  2889-2896 .  doi:10.3864/j.issn.0578-1752.2010.14.007
    Abstract ( 929 )   PDF (339KB) ( 894 )   Save
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    【Objective】 Stripe rust of wheat (Triticum aestivum L.), caused by Puccinia striiformis f. sp. tritici, has been considered one of the most damaging for wheat production in Northwest China, North China, and the Middle and Lower Reaches of Changjiang River wheat region in China. The objective of this study was to evaluate the resistance levels of current wheat and advanced lines to this disease in the target epidemic region. 【Method】 A total of 501 entries of wheat cultivars and advanced lines, collected from thirteen provinces of China, were evaluated for stripe rust resistance of seedlings in greenhouse with selected races (CYR32 and CYR33), and of adult plants in the field with eight mixed races at Yangling, and in fields under natural infections at Tianshui, during 2008-2009 wheat growth seasons. 【Result】 Among the 501 cultivars or advanced lines, there were only 34 (6.8%), and 110 (22.0%) entries that were resistant at seedling and adult plant stages respectively. A high frequency of cultivars and advanced lines (71.0%) showed a susceptible reaction (infection type ≥ 3 ) in the field at Tianshui in Gansu. There were significant differences in the resistance level of wheat entries among the different epidemic regions. According to the analysis of pedigree, the application of resistant sources of current wheat cultivars to stripe rust among different epidemic regions were focused on a little major effective Yr genes, such as Yr26/24, Guinong resistance lines, and some CIMMYT resistance lines. 【Conclusion】 The resistance levels of current cultivars to stripe rust were very low, and the forecast for epidemic situation of stripe rust must be enhanced in order to avoid stripe rust epidemic in large area. The available sources of rust resistance were too less in wheat breeding programs, and simultaneous utilization in different epidemic regions.

    Induction and Characteristics of Fusarium oxysporum f. sp. fragariae ZY-W Resistant to Tebuconazole
    GU Chun-bo,JIANG Li-li,WANG Kai-yun,SHI Xiao-bin,DUAN Hai-ming,LIN Cai-hua
    Scientia Agricultura Sinica. 2010, 43(14):  2897-2904 .  doi:10.3864/j.issn.0578-1752.2010.14.008
    Abstract ( 1142 )   PDF (283KB) ( 676 )   Save
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    【Objective】 The aim of the study is to induce the resistant isolate of Fusarium oxysporum f. sp. fragariae by in vitro cultivation on media containing tebuconazole and compare the biological characteristics between the sensitive and resistant isolates. 【Method】 The resistant isolate was selected on the media containing tebuconazole. By measuring the colony diameters, the characteristics of the resistant and sensitive isolates and the cross resistance of the resistant isolate to some fungicides were studied. 【Result】 The results showed that the resistance of the induced Fusarium oxysporum f. sp. fragariae isolate to tebuconazole reached 34.22 folds after 36-times selection compared with the sensitive isolate. The resistant level of tebuconazole-resistant isolate was stable. Meanwhile, the positive cross resistance to triadimefon and 6 other fungicides was reported. The optimal temperature was 25 ℃, and the optimum pH value was 9 for both sensitive and resistant isolates. The effects of different carbon and nitrogen sources on the growth and the spore production were different. 【Conclusion】 Fusarium oxysporum f. sp. fragariae is susceptible to produce resistant population in environment, and the resistance risk of Fusarium oxysporum f. sp. fragariae needs to be noticed in production.

    SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT
    Nitrogen Absorption and Allocation of Spring Maize on Dryland of Loess Plateau in Different Farmland Water Management Patterns
    XU Hong-min,ZHU Lin,LIU Yi,CHEN Xin-ping,LI Shi-qing
    Scientia Agricultura Sinica. 2010, 43(14):  2905-2912 .  doi:10.3864/j.issn.0578-1752.2010.14.009
    Abstract ( 874 )   PDF (313KB) ( 722 )   Save
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    【Objective】 A field experiment was carried out on typical Hap-Ustic Isohumisol soil on dry highland of loess plateau to study the absorption and translocation of nitrogen in different farmland water management patterns under the same N fertilizer rate, therefore, the result would have good application values in guiding high yield cultivation of maize. 【Method】 Four field water management practices, including supplementary irrigation, rain-fed, corn straw mulching and plastic film mulching, were adopted in spring maize Xianyu 335. The amount and proportion of N from different management practices at 6 growing stages (seedling, jointing, tasseling, blister, dough and maturity) were measured. 【Result】 The experimental results showed that with the growing stage increasing, N absorption increased. After tasseling stage, with the center of growth changing the absorption quantity of organs absorbing N was changed. In every growing stage the N amount in supplementary irrigation treatment was higher than other three treatments, followed by plastic film mulching treatment, rain-fed treatment and corn straw mulching treatment. In supplementary irrigation treatment, the amount of organs N translocation was 235.5 kg?hm-2, higher than other three treatments, followed by 225.3 kg?hm-2 in plastic film mulching treatment; straw mulching treatment was higher than rain-fed treatment, but the difference was not significant. Supplementary irrigation treatment and plastic film mulching treatment were significantly higher than other two treatments. 【Conclusion】 Under the conditions of this experiment, in dry highland of loess plateau area, different farmland water management patterns had significantly effects on spring maize nitrogen absorption, distribution and translocation. The management patterns which can increase the yield, the water utilization efficiency and the nitrogen utilization efficiency can also increase the N absorption and the grain N content. Supplementary irrigation treatment and plastic film mulching treatment were better treatments in this experiment. As for the comprehensive water saving and rainfall use efficiency, the recommended management practice should be the plastic film mulching treatment based on the results of this experiment.

    The Depth Variation Characteristics of Soil Potassium in Continuous Cotton Field and Its Changes with Different Cultivation Practices
    HAN Chun-li,LIU Mei,ZHANG Wang-feng,LIU Juan,GAN Xiu-xia,HAO Quan-sheng
    Scientia Agricultura Sinica. 2010, 43(14):  2913-2922 .  doi:10.3864/j.issn.0578-1752.2010.14.010
    Abstract ( 839 )   PDF (379KB) ( 770 )   Save
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    【Objective】 This study was undertaken to elucidate the consumption characteristics of soil potassium, and clarify the effects of different tillage practices on the distribution of potassium in soil profile under cotton monoculture conditions. 【Method】 Some cotton monoculture fields, 1-year cropping, 5-year cropping, 15-year cropping, 20-year cropping, 30-year cropping and 30-year cropping with deep tillaged or rice-cotton rotated practice, were adopted to analyse the variation characteristics of total K and available K in 0-100 cm depth of soil profile. 【Result】 From surface to deep soil, the contents of total K and available K decreased to minimum content at 40-50 cm soil layer, then increased under cotton monoculture conditions. There was distinctive content of total K and available K in soil vertical layer related to different cropping years. With the increase of cropping year, the content of total K and available K were increased and then decreased, continuous decreased and increased in 0-20 cm, 20-70 cm and 70-100 cm soil vertical, respectively. When deep tillaged practice was adopted, the content of total K was even in 0-60 cm soil vertical and the release degree of available K was enhanced. Thus, the content of available K was high during the cotton development stage in middle soil vertical. When rice-cotton rotation practice was adopted, the content of total K and available K accumulated remarkably in deep soil layer from surface layer, and eluviation-illuviation was increased. Thus, the content of available K was low in 0-40 cm soil layer at the following cotton development stage. 【Conclusion】 Many of K, about 63 kg?hm-2?year-1, was carried away through seed cotton from soil. The consumption of K was severe in middle layer of sandy loam soil under cotton monoculture conditions, especially 40-50 cm layer. However, using deep tillaged practice, the distribution of K was changed in soil layer. Furthermore, the content of K was enhanced in middle soil layer and the release of available K was accelerated in soil. On the other hand, using rice-cotton rotation practice, leaching loss of K was increased from soil. Therefore, it was recommend that application of K should be reinforced during the following cotton development stage under monoculture conditions using rice-cotton rotated practice in sandy loam soil.

    CH4 and N2O Emission from Rice Field and Mitigation Options Based on Field Measurements in China:An Integration Analysis
    SHI Sheng-wei,LI Yu-e,LIU Yun-tong,WAN Yun-fan,GAO Qing-zhu,ZHANG Zhong-xin
    Scientia Agricultura Sinica. 2010, 43(14):  2923-2936 .  doi:10.3864/j.issn.0578-1752.2010.14.011
    Abstract ( 1178 )   PDF (493KB) ( 1488 )   Save
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    【Objective】 By integrated analyzing field experimental results about CH4 and N2O emissions from rice field in China, the authors estimated the mitigation potential of various factors controlling CH4 and N2O emissions in order to provide some suggestions for reducing them in paddy field. 【Method】 A database of CH4 and N2O emissions from rice field in China was constructed in order to study the characteristics of two greenhouse gas emissions and analyze the factors which have a significant effect on them. 【Result】 There clearly existed a zonal distribution law of CH4 emission in China, which showed that the CH4 emissions from Southwest of China were far more than other rice planting areas. It was considered that the factors controlling CH4 emission were water management regimes in rice growing period, water status of non-rice growing season, types of organic or synthetic nitrogen fertilizer and levels of chemical nitrogen fertilizer input (P<0.05). Compared to continuously flooded (F), flooding-midseason drainage-reflooding (F-D-F), flooding-midseason drainage-reflooding-moist intermittent irrigation, but without water logging(F-D-F-M)and alternative flooding and drainage after transplanting, or moister irrigation but without water logging(M)reduced CH4 emission by 45%, 59% and 83%, respectively. Water status of non-rice growing season determined by cropping system also has a significant effect on CH4 emission. The mode of short drainage (SD), long drainage (LD), and two drainage (TD) could reduce CH4 emission by 42%-56% compared to flooded (F). Simulated effect of organic amendments on CH4 emission from rice field was decreased in the order of crop straw + farm manure (S+FM)>green manure (GM)>farm manure (FM)>crop straw (S)>composted farm manure and biogas digestion (CM). The effect of different types of chemical nitrogen fertilizer on CH4 emission was unclear. The CH4 emission decreased gradually with increasing chemical nitrogen fertilizer rate (N). When N was 0<N≤150 kg N?hm-2,150<N<250 kg N?hm-2 and N≥250 kg N?hm-2,CH4 emission was reduced by 12%, 29% and 65% compared with treatment of non-fertilizer. There did not exist a zonal distribution law for N2O emission. The factors including water management regimes in rice growing period, the non-rice season water status, and total nitrogen fertilizer input were important for N2O emission (P<0.05). Compared to CF, the N2O emission was increased by 12%, 140% and 478% under F-D-F, F-D-F-M and M. The nitrogen fertilizer-induced N2O emission factors were 0.43% and 0.68% in F-D-F and F-D-F-M, respectively. By analyzing the water status of non-rice growing season, the average N2O emission increased by 40%-110% under SD, LD and TD, compared to F. 【Conclusion】 There was a trade-off relationship between emission of CH4 and N2O from rice field, appeared water management regimes in rice growing period, water status of non-rice growing season, and the amount of synthetic nitrogen fertilizer. Mitigation measurements in rice fields should be taken consideration comprehensively both of CH4 and N2O emissions. Global worming potential of CH4 and N2O emission from rice field could be reduced by optimizing water regime and fertilizer management.
    HORTICULTURE
    Generation and Identification of 5# Chinese Cabbage-Cabbage Disomic Alien Addition Lines
    MENG Ya-ning,WANG Yan-hua,GU Ai-xia,LI Xiao-feng,SHEN Shu-xing
    Scientia Agricultura Sinica. 2010, 43(14):  2937-2943 .  doi:10.3864/j.issn.0578-1752.2010.14.012
    Abstract ( 851 )   PDF (639KB) ( 686 )   Save
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    【Objective】 The objective of this study is to obtain homozygous Chinese cabbage-cabbage disomic alien addition lines. 【Method】 In this study, 5#Chinese cabbage-cabbage monosomic addition line was used, and its selfing F1 generation was tested by means of identification of cytology and SSR analysis. 【Result】 The results showed that the ratio of disomic addition lines (2n=22) was 10.81% in 37 screened plants of selfed progeny. Through karyotype analysis and molecular marker identification, 4 Chinese cabbage-cabbage disomic alien addition lines (Chinese cabbage with 5# chromosome from cabbage) were identified in the plants with 2n=22. The alien chromosomes of disomic alien addition lines moving late was observed at anaphaseⅠof meiotic behavior, but the distribution of most of the chromosomes was 11-11-11-11 at anaphaseⅡ, the ratio was 69.55%. 【Conclusion】 5# Chinese cabbage-cabbage disomic alien addition lines were obtained. The alien chromosome of 5# Chinese cabbage-cabbage disomic alien addition lines corresponded with 07 linkage group of cabbage. Disomic alien addition lines undergo meiosis to form the tetrad which is mainly additional one chromosome of the cabbage (n=11), it has higher hereditary stability. The acquisition of alien addition lines for the study of the phylogenetic relationships between brassica A and C genome, and for transmitting valuable genes from cabbage to Chinese cabbage and expanding the genetic background of Chinese cabbage germplasm, are of great significance.

    Resistance Genes Screening in Cycled Uncinular necator Induced cDNA Library of Vitis pseudoreticulata
    ZHANG Jun-ke,DU Jing,LI Shuang,ZHU Zi-guo,WANG Yue-jin
    Scientia Agricultura Sinica. 2010, 43(14):  2944-2952 .  doi:10.3864/j.issn.0578-1752.2010.14.013
    Abstract ( 794 )   PDF (515KB) ( 725 )   Save
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    【Objective】 This research aimed to screen resistance genes from cycled Chinese wild grape Vitis pseudoreticulata cv. Baihe35-1 cDNA library which was constructed from leaves induced by grape powdery mildew (Uncinular necator) inoculation. 【Method】Specific oligonucleotide primers were designed according to P-loop region of conservative NBS and LRR sequence of resistance genes and the universal sequence primers on cloning vector. According to the method of three dimensional library, colony PCR was employed to screen the circled cDNA library. 【Result】 Twenty-four super pools, each contains 768 colonies, were built, obtaining 18 432 colonies, and 585 positive colonies were obtained, in which 330 by NBS primers and 255 by LRR primers. The sequencing results were assembled into 422 contig sequences that 250 and 172 sequences from NBS and LRR primer screening respectively, including 25 sequences from the both. 【Conclusion】 There were 8 sequences (GW787758, GW787739—GW787742, GW787746, GW787747, GW787749) belong to P-loop superfamily and 5 sequences (GW787743, GW787744, GW787751, GW787753, GW787750) with LRR and a specific gene in Vitis pseudoreticulata (GW787754). Ten sequences which have different resistance domains were chosen for real-time quantitative PCR to check the expression profile in the process of white powdery mildew invasion. Five showed significant variation during Uncinular necator induction and supposed to be a pathogenesis related gene.

    Heterosis and Mixed Genetic Analysis of Inflorescence Traits of Chrysanthemum

    ZHANG Fei,CHEN Fa-di,FANG Wei-min,CHEN Su-mei,LI Feng-tong
    Scientia Agricultura Sinica. 2010, 43(14):  2953-2961 .  doi:10.3864/j.issn.0578-1752.2010.14.014
    Abstract ( 882 )   PDF (381KB) ( 760 )   Save
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    【Objective】 Inflorescence traits are an effective representation of ornamental merit in chrysanthemum. Heterosis and genetic analysis of inflorescence traits will provide an invaluable guidance for the breeding program of chrysanthemum with excellent ornamental traits. 【Method】 In this study, inheritance and heterosis analysis were carried out for 6 inflorescence traits of chrysanthemum based on phenotypic data in 2008-2009 of 142 F1 population derived from autumn-flowering chrysanthemum cultivar ‘Yuhualuoying’ with single flowers (female parent) and summer-flowering chrysanthemum cultivar ‘Aoyunhanxiao’ with double flowers (male parent) using single generation segregation analysis method of major gene plus polygene mixed genetic model. 【Result】 Compared with mid-parent value (MPV), the heterosis value of mid-parent (Hm) for the inflorescence traits of flower head diameter, ray floret number, ray floret length, ray floret width and center flower diameter, except tubular floret number, showed a significant difference degree at 0.01 level, and the ratio of heterosis value of mid-parents (RHm) for the first five traits were -3.19%, -25.17%, -4.46%, -12.81%, and 5.06%, respectively. The mixed genetic analysis revealed that there was no major gene detected for ray floret length and width, and flower diameter was fitting A-1 model with additive effect (0.618) larger than its dominant effect (0.168); ray floret number accorded with B-2 model with additive-dominant effect, additive effect of the first major gene (24.575) was larger than that of the second (13.120) and the dominant effect of the two was null; tubular floret number was fitting A-4 model with negatively complete dominant effect; center flower diameter were apt to B-3 model with additive effect. The heritability of major gene for the four inflorescence traits of flower diameter, ray floret number, tubular floret number and center flower diameter were 66.69%, 80.99%, 58.24% and 56.49%, respectively. 【Conclusion】 The heterosis and transgressive segregation of inflorescence traits commonly existed in F1 hybrid progenies of chrysanthemum and there were some dominant effect in the heterosis of flower diameter, ray floret number, ray floret length and width. Major genes with dominantly additive gene effects were detected for flower head diameter, ray floret number, tubular floret number and center flower diameter, and consequently, the detection of these major genes controlling inflorescence traits will afford a theoretical basis for the further study of QTL analysis and molecular marker assisted breeding program in chrysanthemum.
    STORAGE·FRESH-KEEPING·PROCESSING
    Effect of 1-Methylcyclopropene and Nitric Oxide on Main Fruit Quality of ‘Whangkeumbae’ Pear and Related Enzymes of Fatty Acid Metabolism During Storage
    TIAN Chang-ping,WANG Yan-ling,LIU Zun-chun,WANG Chuan-zeng,SUN Jia-zheng,WANG Na,CHEN Xue-sen
    Scientia Agricultura Sinica. 2010, 43(14):  2962-2972 .  doi:10.3864/j.issn.0578-1752.2010.14.015
    Abstract ( 815 )   PDF (356KB) ( 861 )   Save
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    【Objective】 Effects of 1-methylcyclopropene and nitric oxide treatments on main fruit quality of ‘Whangkeumbae’ pear and related enzymes of fatty acid metabolism during low temperature storage were investigated. The action mechanisms of aroma were explored so as to provide a theoretical basis for fresh-keeping of ‘Whangkeumbae’ pear during storage. 【Method】 ‘Whangkeumbae’ pear fruits at the commercial ripe stage were fumigated with concentrations of 1-methylcyclopropene (1 μL?L-1) for 18 hours and nitric oxide gas (10 μL?L-1) for 2 hours, respectively. Changes of firmness, ethylene production, aroma compounds contents, and the activities of lipoxygenase (LOX), hydroperoxide lyase (HPL), alcohol dehydrogenase (ADH) and alcohol acyltransferase (AAT) were investigated. 【Result】 The results demonstrated that the evolution of ethylene was obviously reduced, the reduction of firmness, total alcohols and total aldehydes were obviously delayed, the increase of total esters was delayed by 1μl?L-1 1-methylcyclopropene and 10μl?L-1 nitric oxide treatments, but aroma components had some differences between 1-methylcyclopropene and nitric oxide treatments. The activities of LOX, HPL, ADH and AAT were also inhibited significantly. 【Conclusion】 The treatments of 1-methylcyclopropene (1 μL?L-1) for 18 hours and nitric oxide gas (10 μL?L-1) for 2 hours, respectively, can maintain higher storage quality of the ‘Whangkeumbae’ pear fruits, and the effect of nitric oxide (10 μL?L-1) for 2 hours is better than 1-methylcyclopropene (1 μL?L-1) for 18 hours treatment.

    Progress in Research of Mechanisms of Astringency Removal and Postharvest De-Astringency Handles of Persimmon Fruits
    LI Bao,SHANG Li,XUE Xiao-li,ZHANG Yin
    Scientia Agricultura Sinica. 2010, 43(14):  2973-2981 .  doi:10.3864/j.issn.0578-1752.2010.14.016
    Abstract ( 1026 )   PDF (249KB) ( 872 )   Save
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    Along with the depth of understanding of healthy and medicine benefits, the cultivation area and production of persimmon are increasing year by year. Postharvest physiology of persimmon (Diospyros kaki), especially in the mechanism of astringency removal and postharvest handles, are the critical aspects of persimmon industry development in China. The mechanisms of astringency removal of persimmon fruits belonging to different groups and postharvest de-astringency handles of astringent fruits were reviewed in this paper, aiming to provide some information to future researches.

    Protective Effects of Whey Protein Isolate (WPI) Peptides on Lung Fibroblasts Cell MRC-5 Caused by the Damage of H2O2
    PENG Xin-yan,KONG Bao-hua,XIONG You-ling
    Scientia Agricultura Sinica. 2010, 43(14):  2982-2989 .  doi:10.3864/j.issn.0578-1752.2010.14.017
    Abstract ( 1080 )   PDF (831KB) ( 946 )   Save
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    【Objective】 A study was conducted to investigate the protective effects of purified whey protein isolate (WPI) peptides P4 on lung fibroblasts cell MRC-5 caused by the damage of H2O2 and its protective mechanism. 【Method】 Oxidative damage was induced by H2O2, and cell viability was measured by the 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide (MTT) assay. The influence of WPI peptides P4 on H2O2-induced MRC-5 injury was assessed by measuring the superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities and the malondialdehyde (MDA) content. The change of cell morphology was observed by scanning transmission electron microscopy. 【Result】 The results demonstrated that MRC-5 cells were damaged by incubation with 1 mmol?L-1 H2O2 for 24 h, and the viability of MRC-5 cells reduced to 22.47%. The addition of WPI peptides P4 (4, 20, and 100 μg?mL-1) into the MRC-5 cell suspensions prior to the exposure to 1 mmol?L-1 of H2O2 resulted in a greater survival rate of the cells. In particular, the cell viability reached 44.77% after treating with 100 μg?mL-1 WPI peptides P4. Moreover, at elevated concentrations, WPI peptides P4 exhibited increased repairing capability for injured MRC-5 as well as increased protection of SOD, CAT and GSH-PX while reducing MDA formation. Scanning transmission electron microscopy also showed that WPI peptides P4 had the protection on MRC-5. 【Conclusion】 The purified WPI peptides P4 possess protective effects on MRC-5 cell injuries induced by H2O2, and this may be related to the antioxidative activity of WPI peptides.

    ANIMAL SCIENCE·RESOURCE INSECT
    Analysis of Association Between DGAT1 Gene and Milking Traits in Chinese Holstein in the South of China
    MAO Yong-jiang,CHEN Ren-jin,CHEN Ying,CHANG Ling-ling,SHI Xue-kui,YANG Zhang-ping,SHI Jian,HUANG Hui-hua
    Scientia Agricultura Sinica. 2010, 43(14):  2990-2995 .  doi:10.3864/j.issn.0578-1752.2010.14.018
    Abstract ( 827 )   PDF (404KB) ( 913 )   Save
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    【Objective】 Analysis of association between DGAT1 gene and milking traits for Chinese Holstein in the south of China was investigated. 【Method】 The polymorphism of the K232A site in exon 8 of DGAT1 gene was investigated in 605 Chinese Holstein cows from 30 bull families in Jinshan Dairy Farm of Shanghai Bright Dairy Group using PCR-SSCP, and with a mixed animal model, the effects of the polymorphism of the K232A site on test-day milk yield, fat percentage, milk protein percentage, 305 days corrected milk yield, milk fat yield, milk protein yield and SCS were analysed. 【Result】 Three genotypes were found and the genotypic frequencies of KK, KA and AA were 0.7918, 0.1917, and 0.0165, respectively. The gene frequencies of K and A were 0.8876 and 0.1124. The very significant association of the DGAT1 K232A with tested day milk yield, milk fat percentage, 305 days corrected milk yield and milk protein yield (P<0.01), and significant association with milk fat yield were identified, and had no significant effect on the SCS in milk (P>0.05). LSD showed that the cows with KK and KA genotypes had higher tested day milk yield, 305 days corrected milk yield and milk protein yield than those with AA genotype (P<0.01),while had lower milk fat percentage and milk protein percentage (P<0.01). 【Conclusion】 The DGAT1 K232A subsitution has a great genetic effect on milking traits and could be a useful genetic marker for Chinese Holsteion breeding.

    Identification of the Regulation Sequences of Bovine Sry Promoter
    HAN Feng-tong,LIN Xiu-kun,LIU Di,WU Ning,LIAO Bing
    Scientia Agricultura Sinica. 2010, 43(14):  2996-3004 .  doi:10.3864/j.issn.0578-1752.2010.14.019
    Abstract ( 787 )   PDF (698KB) ( 602 )   Save
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    【Objective】 Sry is the pivotal gene initiating male sex determination in most mammals, but how its expression is regulated is still not clear. The study was to identify the regulation sequences of bovine Sry promoter, and to understand the transcriptional regulation of the Sry. 【Method】 A 1056 bp long bovine Sry 5'-flanking sequences, and the potential transcriptional start sites in this region were predicted by bioinformatics method as well as reporter gene analysis was isolated, Bovine genital ridge cells were isolated and deletion recombinant plasmids with different lengths of Sry 5' flanking regions were constructed. Sry 5'-flanking promoter activity analysis was performed in bovine genital ridge cells by the dual-luciferase reporter assay system.【Result】 The results indicated that the genital ridge cells cultured in vitro could express the characteristic genes of male genital ridge, including Sry, Sox9, Sf-1 and Dax1. Through the prediction analysis, three potential transcription start sites for bovine Sry were identified at -93, -419 and -722 upstream from the translation initiation site. Further analysis demonstrated that a 35 bp long fragment (-599 to -565) in the 5'-flanking sequence was important for the basic transcriptional activity. Prediction of the 35 bp long fragment demonstrated that there were several potential transcription factor binding sites in this region. 【Conclusion】 There were several potential regulation sequences existed in the 35 bp long fragment (-599 to -565) of bovine Sry 5'UTR。

    Phylogenetics of Domestic Sheep and Multi-Horned Sheep Based on cytb Gene

    ZHAO Qian-jun,GUAN Wei-jun,QIAO Hai-yun,MENG Xiang-ren,HAN Jian-lin,LI Xiang-chen,HE Xiao-hong,PU Ya-bin,MA Yue-hui
    Scientia Agricultura Sinica. 2010, 43(14):  3005-3011 .  doi:10.3864/j.issn.0578-1752.2010.14.020
    Abstract ( 784 )   PDF (303KB) ( 808 )   Save
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    【objective】 The aim of the study is to investigate the origin and evolution of Chinese domestic sheep (ovis aries) and the relationship between domestic sheep and multi-horned sheep, 【Method】 The complete cytb sequences of 42 individuals from 8 provinces were sequenced. 【Result】 The result showed that totally 40 haplotypes were detected, and Haplotype diversity (Hd) and nucleotide diversity (?) were 0.997±0.006 and 0.793%, respectively. The neighbour-joining tree indicated that there are four distinct lineages (A, B, C and D) in Chinese domestic sheep. It is firstly reported that lineage D were detected in Chinese sheep. And lineages B and C had the highest variance value, which diverged from each other about 595±150 million years. The multi-horned sheep belonged to lineages A, B and D, which had closer relationship with domestic sheep than wild sheep. 【Conclusion】 Analysis of phylogenesis shows that Chinese sheep have multiple maternal origins or ever experienced several times domestication possibly. There is no evidence that urial, argali and asian mouflon had molecular contribution to the maternal ancestor of Chinese sheep.

    VETERINARY SCIENCE
    Establishment of Porcine Parvovirus Detection by Loop-Mediated Isothermal Amplification Assay
    LIU Ye-bing,ZHANG Lei,NING Yi-bao,WANG Qin,FAN Xue-zheng
    Scientia Agricultura Sinica. 2010, 43(14):  3012-3018 .  doi:10.3864/j.issn.0578-1752.2010.14.021
    Abstract ( 798 )   PDF (396KB) ( 762 )   Save
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    【Objective】 The objective of the study is to establish a rapid detection of porcine parvovirus (PPV) by a loop-mediated isothermal amplification assay (LAMP). 【Method】 According to the published GenBank sequences, many pairs of primers were designed targeting the conserved region of PPV. The amplification was detected with LAMP Real Time Turbidimeter LA-320. Through optimizing the LAMP primers and reaction conditions, a rapid and specific detection of PPV was established. Meanwhile, the amplified products were colored by SYBR green I after completion of the reaction, so that the amplification could be detected with naked eyes. 【Result】 The method of LAMP shows a highly efficient amplification for PPV viral target gene which performed at 63 ℃ for 45min by the LAMP Real Time Turbidimeter LA-320. The detection limit was 0.23 TCID50. The results of PPV LAMP reaction visualized the tube directly with naked eyes by the addition of SYBR GreenⅠare consistent with the results detected by Tubidimeter real-time. Twenty clinical samples were detected by LAMP, PCR and IF, and the coincidence rate was 20/20. 【Conclusion】 PPV LAMP detection method established by the authors is rapid, specificity, high sensitivity, be easy of operation under simple conditions, which is suitable for rapid detection.

    Identification of Euroasiatic and South Africa Serotype Foot-and-Mouth Disease Virus by One-Step Multiplex Real-Time Quantitative RT-PCR Assay
    WU Shao-qiang,ZHA Cheng-gang,DENG Jun-hua,LIN Xiang-mei,LIU Jian,MEI Lin
    Scientia Agricultura Sinica. 2010, 43(14):  3019-3026 .  doi:10.3864/j.issn.0578-1752.2010.14.022
    Abstract ( 810 )   PDF (637KB) ( 712 )   Save
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    【Objective】 Foot-and-mouth disease (FMD) is a serious political and economic disease worldwide and is caused by foot-and-mouth disease virus (FMDV). In order to improve the diagnostic speed and accuracy of FMDV, especially for the early detection of FMDV transmission on the boundary quarantine, the one-step real-time quantitative RT-PCR (RT-qPCR) method was established to detect Euroasiatic serotype (EAT) and South Africa serotype (SAT) FMDV simultaneously. 【Method】 Aiming to amplify the conserved 5' untranslated region (UTR) of FMDV genomic sequence, the specific primers(U6/L6)and probe(P6) for EAT, primers (AY48U/L) and probe (AY48P) for SAT of FMDV were designed to establish a multiplex qPCR detection method for FMDV. The amplification solution and reaction conditions were optimized using plasmid inserted with target DNA fragment as template. To offer control for the established qPCR assay, the respective cRNAs were achieved by in vitro transcription using T7 promoter ligated PCR amplicons as template. The sensitivity and specificity of the established assay were then verified using the subsequent cRNA as template. The field samples were adopted to examine the efficiency of the final established FMDV one-step multiplex RT-qPCR detection method. 【Result】 The amplification efficiency was both up to 90% in FMDV multiplex qPCR assay using EAT and SAT plasmids as targets. The in vitro transcription cRNAs, tested to be without plasmid DNA residual, were used to be as positive control for EAT and SAT FMDV one-step multiplex RT-qPCR. The detection limit was 7.6×10-9ng cRNA for SAT and 6.3×10-8ng cRNA for EAT, respectively. Specificity test indicated that EAT and SAT FMDV could be distinguished efficiently using the developed method. The examining result was in accordance with the actual infection status of the field samples.【Conclusion】The established EAT and SAT FMDV one-step multiplex RT-qPCR assay will supply an effective approach to distinguish EAT from SAT FMDV contamiantion and for thd use of FMDV epidemiology investigation.

    Diversification of Porcine Pulmonary Surfactant Protein A and Its Significance in Acute Lung Injury
    QIAO Li-juan,WANG Li-xian,WANG Li-gang,YAN Hua,ZHANG Long-chao
    Scientia Agricultura Sinica. 2010, 43(14):  3027-3035 .  doi:10.3864/j.issn.0578-1752.2010.14.023
    Abstract ( 927 )   PDF (383KB) ( 911 )   Save
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    【Objective】 To discuss the significance of porcine pulmonary surfactant protein A (SP-A) in acute lung injury (ALI) and in veterinary clinical diagnoses and cure porcine respiratory disease (PRD), diversification of SP-A concentration in porcine serum and branchoalveolar lavage fluid (BALF), and relationship between the SP-A concentration and the changes of lung water content, which is the index of the severity of lung injury, was assessed. 【Method】 Eighteen healthy Large White piglets of 50±1 days were collected to establish a model of oleic acid (OA)-ALI containing normal saline (NS) group and OA group. At 1, 3, 6 h time point, the SP-A concentration in serum was measured by ELISA technology, and the SP-A concentration in BALF was measured by Western blotting technology, respectively. Also, the correlation between the SP-A concentration in serum and the lung water content, and between the SP-A concentration in BALF and the lung water content were studied by Kendall correlation analysis. 【Result】Compared with the NS group, porcine lung water content of OA group was significantly increased at 1, 6 h time point (P<0.05), and was extremely significantly increased at 3 h time point. In OA group (P<0.01), porcine lung water content at 3 h time point was higher than those at 1 h time point, and porcine lung water content at 6 h time point was lower than those at 3 h time point, but both of the difference was not significant. It was deduced that the 1 h time point fell into protophase of lung injury, lung injury was more severe at 3 h time point, and 6 h time point fell into paracmasis of lung injury. Compared with the NS group, the SP-A concentration in serum of OA group was extremely significantly increased in protophase of lung injury (P<0.01), and that of OA group was higher than those of NS group in severe period and protophase of lung injury, but both of the difference wasn’t prominent. Moreover, the SP-A concentration in serum of OA group in severe period was extremely significantly reduced compared with those in protophase of lung injury (P<0.01), and the SP-A concentration in serum of OA group in paracmasis was significantly reduced compared with those in severe period of lung injury (P<0.05). During different periods of lung injury, the SP-A concentration in BALF of OA group was extremely significantly reduced compared with the NS group (P<0.01). Moreover, the SP-A concentration in BALF of OA group in severe period was extremely significantly reduced compared with those in protophase of lung injury (P<0.01), and the SP-A concentration in BALF of OA group in paracmasis was extremely significantly increased compared with those in severe period of lung injury (P<0.01). Correlation analytic results showed that there was an extremely significantly positive correlation between lung water content and ELISA assaying for SP-A concentration in serum (r=0.50327, P<0.01), and there was extremely significantly negative correlation between lung water content and Western blotting assaying for SP-A concentration in BALF (r=-0.72499, P<0.0001). 【Conclusion】 As the expression of SP-A is not pulmonary tissue specific, there are still some further investigations left that porcine SP-A concentration in serum is taken as a marker for evaluation of the severity of lung injury. Porcine SP-A concentration in BALF could be taken as an index for evaluation of the severity and prognosis of lung injury during ALI. This research has established a primary foundation for clinical diagnoses and cure of PRD, and development of related research through assay for SP-A concentration in porcine serum and BALF.
    RESEARCH NOTES
    Difference of Apoplastic NH4+ Concentration in Flue-Cured Tobacco Varieties with Different Tolerance to Nitrogen Nutrition and Assay of the Related Physiological Parameters
    LIU Hua-bing,YANG Tie-zhao,ZHANG Xiao-quan,DUAN Wang-jun,SHANG Xiao-ying
    Scientia Agricultura Sinica. 2010, 43(14):  3036-3043 .  doi:10.3864/j.issn.0578-1752.2010.14.024
    Abstract ( 786 )   PDF (377KB) ( 1192 )   Save
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    【Objective】 The objective of this study was to elucidate the relationship between the apoplastic NH4+ concentration and the nitrogen tolerance of flue-cured tobacco varieties and to provide a theoretical basis for selecting tobacco variety with different tolerance to nitrogen nutrition. 【Method】 A pot experiment was carried out to study the difference of apoplastic NH4+ concentration in three flue-cured tobacco varieties, ZY90 (tolerance to nitrogen) , K326 (moderate tolerance to nitrogen) and NC89 (sensitive to nitrogen). 【Result】 Apoplastic NH4+ concentration exhibited a steady increase in the three varieties. ZY90 and NC89 had higher and lower apoplastic NH4+ concentration, respectively. A sharp decrease in glutamine synthetase activity, apoplastic pH and total nitrogen content and a gradual increase glutamate dehydrogenase activity were also found. pH in apoplastic solution was lower than that in mesophyll throughout the experimental period. A significant positive correlation was found between glutamate dehydrogenase activity and apoplastic NH4+ concentration. Both glutamine synthetase activity and total nitrogen had a significant negative correlation with apoplastic NH4+ concentration. 【Conclusion】 The tolerance to nitrogen nutrition of flue-cured tobacco variety had correlation with apoplastic NH4+ concentration and the activity of glutamine synthetase and glutamate dehydrogenase at late growth stage. It was deduced that the tobacco variety with high tolerance to nitrogen nutrition has higher apoplastic NH4+ concentration, glutamate dehydrogenase activity and ammonia volatilization and lower glutamine synthetase activity.

    Regression Analysis on Hay Yield and Relative Characters of Multifoliolate Alfalfa and Trifoliolate Alfalfa
    WANG Wen-yue,HAN Qing-fang,ZONG Yu-zheng,JIA Zhi-kuan,NIE Jun-feng,MIN An-cheng,MA Xiao-li
    Scientia Agricultura Sinica. 2010, 43(14):  3044-3050 .  doi:10.3864/j.issn.0578-1752.2010.14.025
    Abstract ( 817 )   PDF (272KB) ( 1038 )   Save
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    【Objective】 The purpose of this study was to provide academic references for the variety improvement and production of alfalfa by analyzing the main characters of hay yield between multifoliolate alfalfa and trifoliolate alfalfa. 【Method】 The growth characters and the net photosynthesis rate(Pn) were mea sured. The regression equation of characters related to the hay yield was established by principal components analysis and stepwise regression analysis. And then, significance test of difference and variance analysis were used for eigenvectors in principal component analysis. 【Result】 The results showed that the hay yield of multifoliolate alfalfa had significant correlation with density of branches, specific leaf weight, dry weight of branches, plant height, number of compound leaves, photosynthesis rate, stem/leaf ratio and fresh/dry ratio (P<0.05). What significantly correlated with the hay yield of trifoliolate alfalfa includes plant height, density of branches, specific leaf weight, internode number, fresh/dry ratio and stem/leaf ratio (P<0.05). The results of significance test of difference in the common characters between two different kinds of alfalfa showed that the density of branches, stem/leaf ratio and fresh/dry ratio in multifoliolate alfalfa were significantly lower than those in trifoliolate alfalfa, specific leaf weight in multifoliolate alfalfa was significantly higher than those in trifoliolate alfalfa (P<0.05). There was no obvious difference in plant height between the two kinds of alfalfa. 【Conclusion】 As a whole, the hay yield of multifoliolate alfalfa was significantly higher than trifoliolate alfalfa. There is a difference in the characters between multifoliolate alfalfa and trifoliolate alfalfa. The same characters in the stepwise regression had different effects on different kinds of alfalfa. The hay produce can by improved by increasing the specific leaf weight and decreasing the fresh/dry ratio and stem/leaf ratio. The density of branches of multifoliolate alfalfa is lower than trifoliolate alfalfa, therefore, the hay yield of multifoliolate alfalfa can be enhanced by increasing the number of branches number and applying rational close planting.