Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (10): 2156-2161 .doi: 10.3864/j.issn.0578-1752.2010.10.023

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Studies on SNP and Genomic Imprinting of the IGF2R Gene in Swine

LI Li, CHEN Yu-ming, BAI Ying-shan, WU Tong-shan, ZHANG Shou-quan
  

  1. (华南农业大学动物科学学院)
  • Received:2009-09-23 Revised:2009-12-08 Online:2010-05-15 Published:2010-05-15
  • Contact: ZHANG Shou-quan

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Abstract:

【Objective】 In this study, single nucleotide polymorphism (SNP) was found in IGF2R exons and used as marker to investigate the imprinting pattern of IGF2R in swine. 【Method】 Gene polymorphism of swine IGF2R was detected from 243 pigs of three breeds by PCR-SSCP. Imprinting patterns of IFG2R exon 48 were analyzed in organs of three heterozygous piglets and one hog by RT-PCR-RFLP/SSCP. 【Result】 One SNP(a G→A transition) was identified in exon 48 of IGF2R , located at the 384th nucleotide (5→3),forming a Nsp I restriction site. Maternal expression of IGF2R exon 48 was observed in the main organs of four heterozygous pigs. 【Conclusion】 IGF2R exon 48 was maternally expressed and paternally imprinted in most tissues of piglets and hogs.

Key words: swine, IGF2R, SNP, genomic imprinting

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