Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (10): 2156-2161 .doi: 10.3864/j.issn.0578-1752.2010.10.023

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Studies on SNP and Genomic Imprinting of the IGF2R Gene in Swine

LI Li, CHEN Yu-ming, BAI Ying-shan, WU Tong-shan, ZHANG Shou-quan
  

  1. (华南农业大学动物科学学院)
  • Received:2009-09-23 Revised:2009-12-08 Online:2010-05-15 Published:2010-05-15
  • Contact: ZHANG Shou-quan

PDF

747

Abstract:

【Objective】 In this study, single nucleotide polymorphism (SNP) was found in IGF2R exons and used as marker to investigate the imprinting pattern of IGF2R in swine. 【Method】 Gene polymorphism of swine IGF2R was detected from 243 pigs of three breeds by PCR-SSCP. Imprinting patterns of IFG2R exon 48 were analyzed in organs of three heterozygous piglets and one hog by RT-PCR-RFLP/SSCP. 【Result】 One SNP(a G→A transition) was identified in exon 48 of IGF2R , located at the 384th nucleotide (5→3),forming a Nsp I restriction site. Maternal expression of IGF2R exon 48 was observed in the main organs of four heterozygous pigs. 【Conclusion】 IGF2R exon 48 was maternally expressed and paternally imprinted in most tissues of piglets and hogs.

Key words: swine, IGF2R, SNP, genomic imprinting

[1] YE MeJin, WU Lei, MD NAHIBUZZAMAN Lohani, YIN Li, HU XinRong, LIU YaXi, JIANG YunFeng, CHEN GuoYue, PU ZhiEn, LI Yang, LI Ting, ZOU YaYa, WU JiaYi, MA Jian. Genome-Wide Association Study-Based Identification of Loci Controlling Mature Embryo Size in Chinese Wheat Landraces and Their Genetic Effects Analysis [J]. Scientia Agricultura Sinica, 2026, 59(6): 1157-1171.
[2] CAO HaiShun, ZHOU DongYuan, WANG Rui, SHI ZhaoWan, WU TingQuan, ZHANG ChangYuan. Identification of Short Hypocotyl Cucumber Germplasm Under Low Light Stress and QTL Mapping of the Trait [J]. Scientia Agricultura Sinica, 2026, 59(6): 1286-1301.
[3] WANG YongSheng, NIU Li, WANG ChangJie, MA LiHua, LIAN XiaoXiao, MENG YaXiong, MA XiaoLe, YAO LiRong, ZHANG Hong, YANG Ke, LI BaoChun, WANG HuaJun, SI ErJing, WANG JunCheng. Genome-Wide Association Study and Candidate Gene Identification for Thousand Grain Weight in Winter Wheat [J]. Scientia Agricultura Sinica, 2026, 59(3): 499-514.
[4] LI YunLi, DIAO DengChao, LIU YaRui, SUN YuChen, MENG XiangYu, WU ChenFang, WANG Yu, WU JianHui, LI ChunLian, ZENG QingDong, HAN DeJun, ZHENG WeiJun. Genome-Wide Association Study of Heat Tolerance at Seedling Stage in A Wheat Natural Population [J]. Scientia Agricultura Sinica, 2025, 58(9): 1663-1683.
[5] LI Ming, CHENG YuKun, BAI Bin, LEI Bin, GENG HongWei. Genome-Wide Association Study on Spike Architecture Traits and Elite Haplotype Mining in Winter Wheat [J]. Scientia Agricultura Sinica, 2025, 58(18): 3583-3597.
[6] HE XiaoPing, ZHANG YuanFeng, LIU XueMin, HUANG Li, WENG ChangJiang. Preparation of Monoclonal Antibody Against African Swine Fever Virus pD345L Protein and Identification of Its Epitope [J]. Scientia Agricultura Sinica, 2025, 58(16): 3345-3356.
[7] LIU HongXiang, ZHANG XuePing, WANG YiFei, WANG ZhiCheng, GU HaoTian, SONG WeiTao, TAO ZhiYun, XU WenJuan, ZHANG ShuangJie, LU LiZhi, LI HuiFang, ZHU ChunHong. Genome-Wide Association Study of Egg Production Traits in Jinding Duck [J]. Scientia Agricultura Sinica, 2025, 58(15): 3145-3158.
[8] WAN YunFei, YANG YuYing, ZHANG NaiXin, XU MengMeng, YU QinHao, QIAO ChuanLing, CHEN HuaLan. Identification and Functional Analysis of Adaptive Amino Acid Mutations in the Eurasian Avian-Like H1N1 Swine Influenza Virus [J]. Scientia Agricultura Sinica, 2025, 58(10): 2035-2044.
[9] ZHANG Ying, SHI TingRui, CAO Rui, PAN WenQiu, SONG WeiNing, WANG Li, NIE XiaoJun. Genome-Wide Association Study of Drought Tolerance at Seedling Stage in ICARDA-Introduced Wheat [J]. Scientia Agricultura Sinica, 2024, 57(9): 1658-1673.
[10] LIU ChuanXia, CHEN Xin, WANG Xiao, LI XueWen, LI TingTing, WENG ChangJiang, ZHENG Jun. Preparation and Application of Polyclonal Antibodies Against Pig CD1d Protein [J]. Scientia Agricultura Sinica, 2024, 57(8): 1620-1628.
[11] ZHAO YiRan, SHAN YanKe, LI JiaHao, HE ZhaoQun, WANG XinYi, WEN Dun, WANG MiLa, CHU Rui, ZHAO DongMing, LIU Fei. Establishment of Rapid Field Co-Detection Method of ASFV Antibody and Nucleic Acid Based on Quantum Dot Microspheres and RPA Technology [J]. Scientia Agricultura Sinica, 2024, 57(24): 4990-5002.
[12] QI XiaoLei, WANG Jun, LÜ GuangDe, MU QiuHuan, MI Yong, SUN YingYing, YIN XunDong, QIAN ZhaoGuo, WANG RuiXia, WU Ke. Genetic Composition Analysis of a New High Quality and High Yield Wheat Cultivar Taikemai33 [J]. Scientia Agricultura Sinica, 2024, 57(22): 4391-4401.
[13] ZHAO WenShuo, ZHANG JinLong, YAO ZhaoRan, SONG YuQi, LÜ Shun, LIU YingXue, YUAN CongCong, SUN YuHang. Effects of Aflatoxin B1 on Influenza Virus Replication, Organ Damages and Intestinal Microbiota Disorder of Swine [J]. Scientia Agricultura Sinica, 2024, 57(20): 4145-4160.
[14] ZHANG MingQi, WANG Rui, ZHANG ChunXiao, SUN Bo, REN Jie, LI ShuFang, WANG Lu, ZHU ShaoXi, ZHANG JiangBin, SHI XinChen, WANG HaiJie, ZHANG YunLong, TIAN HongLi, ZHAO YiKun, KUANG Meng, WANG YuanDong, YI HongMei, LI XiaoHui, WANG FengGe. The Construction and Application of SSR and SNP Molecular ID for Maize Germplasm Resources of Jilin Province [J]. Scientia Agricultura Sinica, 2024, 57(2): 236-249.
[15] FENG ChunYing, ZHANG ZhaoXia, LIU YunFei, HUANG Li, WENG ChangJiang. Preparation of Monoclonal Antibody Against African Swine Fever Virus p54 Protein and Identification of Its Epitope [J]. Scientia Agricultura Sinica, 2024, 57(19): 3936-3944.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd