Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (1): 185-191 .doi: 10.3864/j.issn.0578-1752.2010.01.022

• VETERINARY SCIENCE • Previous Articles     Next Articles

Development of a Novel RT-LAMP Assay for Porcine Reproductive and Respiratory Syndrome Virus

XIN Ting, HOU Shao-hua, JIA Hong, GUO Xiao-yu, DING Jia-bo, LI Yan-peng, DING Min, ZHU Hong-fei
   

  1. （中国农业科学院北京畜牧兽医研究所）
  • Received:2008-11-14 Revised:2009-10-10 Online:2010-01-10 Published:2010-01-10
  • Contact: ZHU Hong-fei

PDF

1339

Abstract:

【Objective】 The objective of the experiment is to develop a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid and sensitive detection of porcine reproductive and respiratory syndrome virus (PRRSV). 【Method】 Three pairs of primers were designed to identify 8 positions in N gene, the sensitivity and specificity of RT-LAMP were determined and clinical test was performed under optimized amplification condition. 【Result】 A RT-LAMP assay was developed for the rapid detection of PRRSV. The process of assay was conducted with one step amplification within 70 minutes and amplification results were visualized. The sensitivity of RT-LAMP assay was 10 000 times higher than RT-PCR. In the detection of 50 porcine serum samples, this assay showed excellent agreement with the standard RT-PCR assay (κ＝0.83). 【Conclusion】 Therefore, the rapid and simple assay is a potential useful technique for PRRSV detection in field condition.

Key words: PRRSV, RT-LAMP, detection

[1] JIAO WenJuan, HE WanLong, GENG HongWei, BAI Bin, LI JianFeng, CHENG YuKun. Stripe Rust Resistance Evaluation and Molecular Characterization of Yr Genes for 155 Spring Wheat Varieties (Lines) [J]. Scientia Agricultura Sinica, 2026, 59(5): 937-950.
[2] GAO YunJie, GUO RuoNan, CHEN ChunWen, DUAN YiFan, ZHANG ZhenJun, NIE ChanChan, LI MengYu, WANG Hui, FENG TingTing, CUI YingYing, DANG GuangHui, LIU SiGuo. Establishment and Preliminary Application of Indirect ELISA Antibody Detection Method for Mycobacterium avium subsp. paratuberculosis in Sheep [J]. Scientia Agricultura Sinica, 2026, 59(3): 655-667.
[3] FU LiJin, CHEN GuanWei, XIAO Gong, WANG XiaoFu, PENG Cheng, CHEN XiaoYun, XU JunFeng, CHEN ZiYan, YANG Lei. A Rapid Detection Method for Genetically Modified Soybean Dbn9004 Based on Dnazyme Signal Amplification [J]. Scientia Agricultura Sinica, 2026, 59(2): 239-249.
[4] LUO Gang, CHENG YiYi, YANG Wen, XIAO YiMeng, YANG ChengXi. CRISPR-Cas12a Gene Editing Technology and Its Application in Agricultural Production [J]. Scientia Agricultura Sinica, 2025, 58(7): 1434-1450.
[5] LI Rui, LIANG Yue, BAI Yang, ZHANG GuiYue, WANG NanNan, QIAO SongLin, ZHANG GaiPing. Research Progress on the Roles and Mechanisms of Autophagy Involved in Porcine Reproductive and Respiratory Syndrome Virus Infection [J]. Scientia Agricultura Sinica, 2025, 58(4): 792-801.
[6] YAN ChuanBo, GONG Ping, ZHENG WenXin, CHEN XinWen, GUO LeiFeng. Research on Sheep Mounting Behavior Recognition in Complex Scenes Based on an Improved YOLOv11 [J]. Scientia Agricultura Sinica, 2025, 58(18): 3783-3798.
[7] ZHANG XiaoQi, SHEN JianGuo, LIAO FuRong, LI WeiMin, JIN YuJie, WUFUER Shayidan, ZHENG LuPing. Establishment and Application of RT-RAA-CRISPR/Cas12a-Based Visual Detection of Prunus Necrotic Ringspot Virus [J]. Scientia Agricultura Sinica, 2025, 58(12): 2371-2381.
[8] HUANG LiQiang, JIANG Ru, ZHU BoZhi, PENG Huan, XU Chong, SONG JiaXiong, CHEN Min, LI YongQing, HUANG WenKun, PENG DeLiang. Identification and Evaluation of Major Potato Cultivars Resistance to Globodera rostochiensis and Detection of Their H1 Resistance Gene Marker [J]. Scientia Agricultura Sinica, 2024, 57(8): 1506-1516.
[9] CHEN XiHong, CAI Wei, YU Yun, LI Min, WANG NianWu, DU ZhenGuo, SHEN JianGuo, GAO FangLuan. Identification of Tea Plant Viruses in Fujian Province and Establishment of Multiplex PCR Detection Assay [J]. Scientia Agricultura Sinica, 2024, 57(4): 698-710.
[10] LI XinLei, SUN JiuYing, YANG Cheng, CHENG Ning, WANG KaiYue, WANG HuanHuan, CHENG XueJiao, ZHAO Jian, SUN YingFeng. Isolation, Identification and Genetic Variation of a Three-Lineage Strain Recombined Porcine Reproductive and Respiratory Syndrome Virus [J]. Scientia Agricultura Sinica, 2024, 57(24): 4978-4989.
[11] ZHAO YiRan, SHAN YanKe, LI JiaHao, HE ZhaoQun, WANG XinYi, WEN Dun, WANG MiLa, CHU Rui, ZHAO DongMing, LIU Fei. Establishment of Rapid Field Co-Detection Method of ASFV Antibody and Nucleic Acid Based on Quantum Dot Microspheres and RPA Technology [J]. Scientia Agricultura Sinica, 2024, 57(24): 4990-5002.
[12] WANG YongJiang, QIAO Qi, WANG Shuang, ZHAO FuMei, TIAN YuTing, ZHANG DeSheng, ZHANG ZhenChen. Establishment and Application of RT-RPA-LFD Detection Method for Sweet Potato Chlorotic Stunt Virus WA Strain [J]. Scientia Agricultura Sinica, 2024, 57(14): 2781-2790.
[13] SU Qi, TANG YaFei, SHE XiaoMan, LAN GuoBing, YU Lin, WU ZhengWei, LI ZhengGang, HE ZiFu. Identification of Viruses Infecting Bottle Gourd in Guangdong Province and Establishment of Multiplex RT-PCR Detection Method [J]. Scientia Agricultura Sinica, 2023, 56(23): 4684-4695.
[14] LI MeiXuan, ZHANG XiangKun, WANG Li, QIAO YueLian, SHI XiaoXin, DU GuoQiang. The Variation of GRSPaV in Different Parts of Shine Muscat Grapevines During Their Phenological Periods [J]. Scientia Agricultura Sinica, 2023, 56(21): 4234-4244.
[15] TANG Wei, ZHANG ChengLing, YANG DongJing, MA JuKui, CHEN JingWei, GAO FangYuan, XIE YiPing, SUN HouJun. Complete Genomic Sequence Characteristics and Establishment of qPCR Detection Technique of Sweet Potato Virus E in China [J]. Scientia Agricultura Sinica, 2023, 56(20): 4010-4020.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd