西藏拉鲁湿地野生植物根际微生物资源挖掘及其在缓解玉米盐碱胁迫中的作用

doi:10.3864/j.issn.0578-1752.2025.20.009

Abstract

Abstract:

【Objective】Soil salinization poses a significant constraint on global agricultural development. However, microorganisms have shown the potential to mitigate salt stress in plants, and long-term intensive cultivation often leads to the unbalance of the beneficial microbial communities in agricultural soils. Isolating plant growth-promoting bacteria from the rhizosphere of wild plants in less disturbed habitats and introducing them into saline-alkali farmland offers a promising strategy to enhance crop tolerance to salt-alkali stress. 【Method】Isolation and culture of rhizospheric bacteria were performed using samples from five wetland plant species collected in the Lalu Wetland National Nature Reserve, Tibet. The isolates were preliminarily identified based on 16S rRNA gene sequencing. Functional characterization was further carried out using specific identification media. Finally, pot experiments were conducted to assess the effects of the selected promising microbial strains on the growth of maize seedlings. 【Result】A total of 260 strains were isolated from the rhizosphere soil of Erigeron canadensis, Galinsoga parviflora, Datura stramonium, Taraxacum mongolicum and Astragalus membranaceus, including 14 species and 21 potential novel species, and the dominant genus was Acinetobacter. 34 strains exhibiting inorganic phosphate-solubilizing capabilities and 47 strains demonstrating siderophore- producing functions were isolated through screening. Among them, strains 3-210 and 3-218 were identified as potential novel species of the genus Flavobacterium through genome analysis, and one key gene for plant hormone signal transduction GS2 was predicted. FeGenie predicted 50 and 71 iron related genes, respectively. No virulence factor genes related to the pathogenic process were detected. At the same time, the results of the pot experiment showed that inoculation of these two strains could improve the agronomic traits, such as plant height and stem diameter of maize seedlings. 3-210 treatment significantly increased the catalase activity and free proline content in leaves, and 3-218 group had significantly higher activities of the catalase and peroxide. 【Conclusion】The rhizospheric soils of plants in the Lalu Wetland National Nature Reserve, Tibet, harbored abundant functional bacterial resources, including multiple strains capable of siderophore production and phosphate solubilization. Notably, two potential novel species of the genus Flavobacterium demonstrated the ability to enhance saline-alkali stress tolerance in maize seedlings.

Key words: Lalu Wetland National Nature Reserve, saline-alkali land, plant growth promoting rhizobacteria, maize, Flavobacterium

YANG Fu, WEI ShanJun, ZHANG XiaoXia, ZHANG LinMin, LIU HongXiu, LI YiJun, TONG YaPing. Characterization and Utilization of Rhizosphere Microbiota from Wild Plants in Lalu Wetland in Xizang for Alleviating Saline-Alkali Stress in Maize[J].Scientia Agricultura Sinica, 2025, 58(20): 4144-4157.

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处理组别 Group	种子萌发试验 Seed germination	幼苗生长试验 Seedling growth
CK+	R2A液体培养基浸种+无盐碱滤纸 R2A broth seed soaking and salt-alkali-free filter paper	R2A液体培养基浸种+60 mL无菌水 R2A broth seed soaking and 60 mL sterilized water
CK-	R2A液体培养基浸种+盐碱浸润滤纸 R2A broth seed soaking and salt-alkal filter paper	R2A液体培养基浸种+60 mL盐碱溶液 R2A broth seed soaking and 60 mL salt-alkali solution
3-210	菌液3-210浸种+盐碱浸润滤纸 Suspension 3-210 seed soaking and salt-alkali filter paper	菌液3-210浸种+60 mL盐碱溶液 Suspension 3-210 seed soaking and 60 mL salt-alkali solution
3-218	菌液3-218浸种+盐碱浸润滤纸 Suspension 3-218 seed soaking and salt-alkali filter paper	菌液3-218浸种+60 mL盐碱溶液 Suspension 3-218 seed soaking and 60 mL salt-alkali solution

处理组别 Group	发芽势 Germination potential (%)	发芽率 Germination rate (%)
CK+	64.28±20.24a	78.57±12.60a
CK-	51.78±24.20b	57.41±18.84c
3-210	59.26±18.84a	64.28±20.25b
3-218	55.54±20.41a	61.90±18.54b

处理组别 Group	盐害程度 Salt damage degree (%)					伤害指数 Injury index (%)
处理组别 Group	1级 Grade 1	2级 Grade 2	3级 Grade 3	4级 Grade 4	5级 Grade 5	伤害指数 Injury index (%)
CK-	50.00	0	27.78	11.11	11.11	46.67
3-210	55.56	11.11	16.67	11.11	5.56	40.00
3-218	55.56	5.56	16.67	11.11	11.11	29.12

Characterization and Utilization of Rhizosphere Microbiota from Wild Plants in Lalu Wetland in Xizang for Alleviating Saline-Alkali Stress in Maize

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