不同氮磷钾处理大豆苗期主根长和侧根数的QTL定位分析

doi:10.3864/j.issn.0578-1752.2017.18.002

Abstract

Abstract: 【Objective】Main root length (MRL) and lateral root number (LRN) are important root traits. It is important to develop the gene resources and reveal the genetic mechanisms of MRL and LRN, and identify quantitative trait loci (QTL) associated with root traits in soybean, including main-effect QTLs, epistatic effects and QTL × environment interactions, meanwhile, map the main-effect QTLs, epistatic effects and QTL × environment interactions in different N, P and K environments. 【Method】A total of 447 RILs derived from a cross between cultivated Jindou23 as the female and native variety HuibuzhiZDD02315 as the male were used as materials. Thirty seeds from each of the RILs and their parents were covered with pasteurized paper, and cultivated in CK (nonfertilized condition), NPK (normal fertilization conditions) and 1.5NPK (high fertilization conditions) at 20-28℃ in 2015 and 2016, and a complete random design with three replications was used in this study. Root traits were measured at V2 stage. Epistatic QTLs and QTL × environment interactions were performed using WinQTLCart 2.5 and QTLNETwork 2.1. 【Result】Twenty-four QTLs for MRL and LRN were detected on chromosomes 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 16 and 17 using CIM method in this study. The variation accounted for by each of these twenty- four QTLs ranged from 8.52% to 43.62%. These QTLs showed additive effect. Two QTLs for MRL and LRN were detected by MCIM, which showed an additive effect. Another two pairs of additive × additive epistatic effects QTLs for MRL and LRN were detected, including one pair of major QTLs and non-major QTL additive × additive epistatic effects, and one pair of non-major QTLs and non-major QTL additive × additive epistatic effects. Two pairs of QTL interaction for MRL explained 1.53% and 1.95% of the phenotypic variation, and two pairs of QTL interaction for LRN explained 2.47% and 1.13% of the phenotypic variation. Two QTLs were simultaneously detected on the same chromosome using two WinQTLCart 2.5 and QTLNETwork 2.1. Nine QTLs were simultaneously detected in three environments. The QTL for MRL was all mapped on chromosome 6 in 2015 (including NPK and 1.5NPK) and 2016 (including 1.5NPK). The QTL for LRN was all detected on chromosome 5 in 2015 (including NPK and 1.5NPK) and 2016 (including CK), another QTL for LRN was all mapped on chromosome 17 in 2015 (including CK and NPK) and 2016 (including NPK). 【Conclusion】MRL and LRN absorb less NPK at seedling stage in soybean, so farmers should minimize the use of NPK in agricultural production. MRL and LRN were controlled by the same controlled gene and specific gene in NPK treatments. Some QTLs were not simultaneously identified in different NPK environments as the related genetic mechanism is comparatively complex. Additive effects, additive × environment interactions and additive × additive epistatic effects are important genetic factors in MRL and LRN formation and inheritance. One each QTL for MRL and LRN was all detected by CIM and MCIM; one stable gene for MRL and LRN existed in interval markers between Satt442-Satt296 and Satt521-GMABABR.

Key words: soybean, nitrogen, phosphorus and potassium, main root length, lateral root number, quantitative trait loci, epistatic effects

LIANG HuiZhen, DONG Wei, XU LanJie, YU YongLiang, YANG HongQi, TAN ZhengWei, XU Yang, CHEN XinWei. QTL Mapping for Main Root Length and Lateral Root Number in Soybean at the Seedling Stage in Different N, P and K Environments[J].Scientia Agricultura Sinica, 2017, 50(18): 3450-3460.

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QTL Mapping for Main Root Length and Lateral Root Number in Soybean at the Seedling Stage in Different N, P and K Environments

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