大豆重组自交系群体NJRSXG百粒重超亲分离的遗传解析

doi:10.3864/j.issn.0578-1752.2015.22.002

Abstract

Abstract: 【Objective】 The present study aimed at exploring the QTL and their allele effects of the 100-seed weight in a recombinant inbred line (RIL) population for revealing the genetic mechanism of transgressive segregation, and providing guidance for breeding for different seed size types in soybeans. 【Method】 The RIL (recombinant inbred line) population NJRSXG derived from a cross between Xinjin 2 and Gantai 2-2 was tested and measured for their 100-seed weight in five environments from 2009 to 2011. The genetic map with 400 SSR markers was established for QTL mapping using the mixed model based composite interval mapping (MCIM) method in QTL Network V2.1 software. Based on the QTL mapping, the QTL-allele constitutions of each line of the RIL population was obtained, and the corresponding QTL-allele matrix was established. 【Result】 The 100-seed weight of the parents, Xinjin 2 and Gantai 2-2, were 16.92 g and 14.14 g, respectively, with those of the RILs ranging from 12.09 g to 25.01 g, showing an obvious transgressive segregation. The genotypic coefficient of variation (GCV) was 16.06%, and the overall heritability was 96.17% from a joint dataset analysis. Ten additive QTL and nine epistatic QTL pairs were detected in the joint dataset using an MCIM method. The phenotypic variation explained by additive QTL was ranged from 0.69% to 14.93%, and the four major QTLs, i.e., Sw-05-2, Sw-08-1, Sw-12-1 and Sw-17-1, were 6.91%, 14.93%, 7.80%, and 5.01%. The Sw-13-3 with both additive effect and epistasis effects had not been reported before. The phenotypic variance of epistatic QTL pairs was small, and ranged from 0.31% to 3.44%. The genetic components of 100-seed weight was estimated from the mapping results, and the analysis of variance for the joint dataset, the genetic contribution due to additive QTL, epistatic QTL pairs, and collective unmapped minor QTL were 47.91%, 13.06%, and 35.19% of the phenotypic variation, respectively. From the mapping procedure, the allele effects of all the loci were also obtained, and the genetic constitution of the QTL-alleles for each line of the RIL population and its parents was used to establish the QTL-allele matrix. The two parents have seven and three loci with positive additive effects respectively; therefore, this is a complementary pair of parents. No lines of the RIL population had all positive alleles or all negative alleles, indicating a hidden potential of recombination, and the lines with larger seed weight had more positive alleles, while the lines with smaller seed weight had more negative alleles, which implies that recombination among loci was the major cause for transgressive segregation of the 100-seed weight in the RIL population. In addition, it was also found that there was still a possibility to improve the 100-seed weight through further recombination. 【Conclusion】 The transgressive segregation of 100-seed weight can be found in a RIL population; 10 additive QTL and 9 epistatic QTL were detected using the joint dataset tested under five environments. The transgressive segregation was caused by recombination among loci between parents, and the potential for further improvement through recombination among RILs still exists.

Key words: soybean, recombinant inbred lines (RIL), 100-Seed weight, linkage mapping, QTL-allele matrix

ZHANG Ying-hu, MENG Shan, HE Jian-bo, WANG Yu-feng, XING Guang-nan, ZHAO Tuan-jie, GAI Jun-yi. The Genetic Constitution of Transgressive Segregation of the 100-Seed Weight in A Recombinant Inbred Line Population NJRSXG of Soybean[J].Scientia Agricultura Sinica, 2015, 48(22): 4408-4416.

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The Genetic Constitution of Transgressive Segregation of the 100-Seed Weight in A Recombinant Inbred Line Population NJRSXG of Soybean

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