Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (9): 1743-1753.doi: 10.3864/j.issn.0578-1752.2014.09.009

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

The Nodule Characteristics and Nitrogen Fixation of Soybean in Maize-Soybean Relay Strip Intercropping

 YU  Xiao-Bo-1, 2 , SU  Ben-Ying-1, GONG  Wan-Zhuo-1, LUO  Ling-1, LIU  Wei-Guo-1, YANG  Wen-Yu-1, ZHANG  Ming-Rong-2, WU  Hai-Ying-2, ZENG  Xian-Tang-3   

  1. 1、College of Agronomy, Sichuan Agricultural University/Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Ya’an 625014, Sichuan;
    2、Nanchong Institute of Agricultural Sciences, Nanchong 637000, Sichuan;
    3、Nanchong Bureau of Agriculture & Animal Husbandry, Nanchong 637000, Sichuan
  • Received:2013-11-13 Online:2014-05-01 Published:2014-03-30

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Abstract: 【Objective】Shoot and root biomass, nodule characteristics, nitrogenase reductive ability and ureide content were studied to investigate the biological nitrogen fixation of soybean under relay-intercropping system.【Method】Three varieties, super-nodulating mutant NTS1007, BRAGG and ND12, were used in this field experiment to investigate the growth characteristics of root nodule, nodule nitrogenase reductive ability and ureide content of plant as well as root and shoot biomass accumulation. 【Result】Compared with monoculture, the results showed that root and shoot biomass decreased significantly in relay strip intercropping system. Nodule biomass was regulated by shoot and root, however, nodule number was correlated with the root biomass. The decreasing amplitude of root biomass (33.15%) was higher than that of shoot (20.84%) in relay strip intercropping system, as a result , the root/shoot ratio was decreased significantly. Furthermore, the nodule nitrogenase reductive ability and the ureide content of plant were also decreased significantly. Compared with monoculture, nitrogenase ability per plant and per weight all decreased, and the declining speed was slower after peak value. The fastest increasing stage of nitrogenase ability per plant came later. Among the three soybean varieties, NTS1007 (52.42%) had a bigger decreasing amplitude in nodule number than others, BRAGG had the biggest decreasing amplitude in root biomass, shoot biomass and nodule weight (37.73%, 31.28%, 43.25%). In maize-soybean relay strip intercropping system, NTS1007 had the biggest decreasing amplitude in nitrogenase activity per plant and ureide content per weight (64.41%, 21.72%), as well as the nitrogenase activity per weight of BRAGG (21.46%), nitrogenase activity and ureide content per plant of ND12 was higher than that of NTS1007 and BRAGG, but the decreasing amplitude compared to monoculture was the lowest among the three soybean varieties (10.01%, 45.81%, 17.88%).【Conclusion】In relay strip intercropping system, shoot biomass, root biomass and root/shoot ratio decreased significantly, as well as the nodule number and nitrogenase ability. The declining speed of nitrogen fixing ability was slower, and this will be favorable to protein accumulation. The decreasing amplitude was different among the three soybean varieties. The biomass accumulation and nodule formation of super-nodulating mutant NTS1007 were sensitive to relay strip intercropping system, but ND12 showed a better adaptability.

Key words: soybean , relay strip intercropping , nodule , nitrogenase , ureide

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