Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (1): 1-8.doi: 10.3864/j.issn.0578-1752.2013.01.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

The Expression Profiling of Rice Calcineurin B-Like Proteins in Seedlings Under Environmental Stresses

 JIA  Lin, LIU  Yu-Meng, FAN  Wei, GUAN  Ming-Li, JIA  Meng, DOU  Shi-Juan, WEI  Jian, PENG  Ye-Bo, LIU  Li-Juan, LI  Li-Yun, LIU  Guo-Zhen   

  1. College of Life Sciences, Agricultural University of Hebei, Baoding 071001, Hebei
  • Received:2012-10-17 Online:2013-01-01 Published:2012-12-12

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Abstract: 【Objective】 The objective of this experiment is to investigate the expression profiling of rice calcineurin B-like (CBL) proteins and the mechanism of stress-tolerance.【Method】The expression patterns of CBL proteins at seedling stage of super hybrid rice parental line 9311 under cold, hot, drought, submerge and salt stresses were surveyed by Western blotting (WB) via antibody-based proteomics strategy. 【Result】The results indicated that the expressions of CBL1, CBL5 and CBL6 were down-regulated under cold stress, the expressions of CBL1 and CBL2 were down-regulated under hot stress, the expressions of CBL1 and CBL5 were down-regulated while CBL6, CBL8 and CBL10 were up-regulated under drought stress. The expressions of CBL1, CBL5, CBL6, CBL8 and CBL10 were up-regulated and CBL2 was down-regulated under submerge stress. 【Conclusion】 Six abiotic stress-related rice CBL proteins were identified.

Key words: rice , western blotting (WB) , stress , calcineurin B-like (CBL) , protein expression

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