贵州白山羊和古蔺马羊脂联素基因多态性及其与繁殖性能的关联研究

doi:10.3864/j.issn.0578-1752.2011.09.019

Abstract

Abstract: 【Objective】The objectives of the present study were to identify the polymorphism of adiponectin gene and its association with reproductive traits in Guizhou White and Gulin Ma goats and to provide a scientific basis for marker-assisted selection for high prolificacy in goats.【Method】According to the sequence of Bos taurus ADIPOQ, four pairs of primers were designed to detect single nucleotide polymorphisms of ADIPOQ in Boer, Chuandong White, Dazu Black, Guizhou White, Jintang Black, Banjiao, Chengdu Ma, Nanjiang Brown, Gulin Ma and Matou goats by PCR-SSCP. The effects of ADIPOQ polymorphisms on the breeding value for litter size and birth weight were examined using least square methods in Guizhou White goats and Gulin Ma goats. 【Result】Only the products amplified by primer P4 displayed polymorphisms of primers P1-P4. For primer P4, three genotypes (AA, AG and GG) were detected in different goat breeds. The sequencing results revealed that the genotype GG had one point mutation (954G→A) compared to AA genotype. The does with genotype GG or AG had 0.18 (P＜0.05) or 0.20 (P＜0.05) kids more than those with genotype AA in Guizhou White goats, respectively. The does with genotype GG or AG had 0.16 (P＜0.05) or 0.15 (P＜0.05) kids more than those with genotype AA in Gulin Ma goats, respectively. In addition, Least squares means for birth weight of different ADIPOQ genotypes had no significant difference (P＞0.05) in Guizhou White or Gulin Ma goats.【Conclusion】The results preliminarily indicated that the ADIPOQ is either a major gene that influences the prolificacy in Guizhou White and Gulin Ma goats or a molecular marker linked with such a gene closely.

Key words: goat , prolificacy , ADIPOQ , PCR-SSCP

LIUChong-xu;WANGPing-qing;ZHANGBao-yun;CHUMing-xing;DENGLa-mei;TANYing;FANQi. Polymorphism of Adiponectin Gene and Its Relationship with Reproductive Ability in Guizhou White and Gulin Ma Goats[J].Scientia Agricultura Sinica, 2011, 44(9): 1916-1922.

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Polymorphism of Adiponectin Gene and Its Relationship with Reproductive Ability in Guizhou White and Gulin Ma Goats

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