Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (24): 4971-4979.doi: 10.3864/j.issn.0578-1752.2011.24.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Creation of Drought-Resistant Variety and Analysis of Physiological Mechanism of W16 Transgenic Wheat

 ZHANG  Shuang-Xi, XU  Zhao-Shi, ZHANG  Gai-Sheng, LI  Lian-Cheng, CHEN  Xiao, CHEN  Ming, MA  You-Zhi   

  1. 1.西北农林科技大学农学院，陕西杨凌 712100
    2.中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程/农业部作物遗传育种重点开放实验室，北京 100081
    3.宁夏农林科学院农作物研究所，宁夏永宁 750105
  • Received:2011-07-21 Online:2011-12-15 Published:2011-10-30

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Abstract: 【Objective】 The objective of this study is to study the breeding of new drought-resistant varieties and analyze their physiological mechanism, and to provide a theoretical basis for drought-resistant identification of transgenic wheat plants. 【Method】 Three stable W16-transgenic lines were used as materials, and drought-resistant identification was done in the field which was compared under irrigation and drought condition from 2008 to 2010. In addition, the contents of proline, souble sugar, and the SPAD value of flag leaf under different growth periods investigated. 【Result】 Under drought stress conditions, the grain weight per plant and the 1000-grain weight of transgenic lines increased significantly by 1% compare with those of the control. The grain weight per plant of transgenic lines increased by 20.00%-23.08%, 20.88%-24.71% and 15.29%-25.27%, respectively, and the 1000-grain weight elevated by 16.24%-19.85%, 13.46%-16.95%, and 21.58%-24.46%, respectively. Under the normal irrigation conditions, the grain weight per plant of the transgenic lines increased significantly by 7.04%-11.11%, 3.52%-8.73%, and 8.45%-12.70%, respectively, and the 1000-grain weight elevated by 13.57%-14.97%, 9.91%-11.67% and 14.17%-14.65%, respectively, compared with the control. Drought resistant index (DRI) of the grain weight per plant and the 1000-grain weight of transgenic lines were strong, or even extremely strong (1.15 to 1.45). In addition, the total length, total surface area, and total volume of root system of transgenic lines were significantly increased under drought stress conditions. The contents of proline and soluble sugar, and SPAD value of flag leaf of transgenic lines were much higher than those of the receptor in the late filling stage.【Conclusion】Overexpression of the W16 improved root structure, prolonged functional period of flag leaf, and finally improved the adaptive ability to drought stress in transgenic wheat lines.

Key words: wheat, drought-resistance, transgene, yield characteristics, physiological and biochemical index

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