Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (17): 3501-3507.doi: 10.3864/j.issn.0578-1752.2011.17.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Analysis of Purple and Waxy Grain in Wheat

HUANG  Bi-Guang   

  1. 福建农林大学作物遗传育种与综合利用教育部重点实验室
  • Received:2010-12-30 Revised:2011-03-21 Online:2011-09-01 Published:2011-04-06

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Abstract: 【Objective】Waxy-grain wheat and purple-grain wheat are special germplasms with special values in nutrition and quality. Based on the genetic law of waxy and purple grain in concrete materials, purple-and-waxy grain wheat was bred which combined the advantages of both wheats.【Method】Reciprocal cross between waxy-grain wheat C75 and purple-grain wheat 03-Chu-3 was made through the F2:3 generation, and then backcross was done between F1 and C75 through BC1F2 generation. The inheritance of grain color and waxiness were determined according to the phenotypic ratios of each generation, and a new wheat germplasm producing purple-and-waxy grains (named as purple-and-waxy wheat) was created from the filial generation. 【Result】 The purple-grain color, behaving as maternal effect, was controlled by 2 pairs of dominant and complementary genes，and the dosage effect was also found for the purple gene, the ratio of 9purple:7red in F2:3 and 1purple:3red in BC1F2 was observed. The waxiness was controlled by 3 independent pairs of duplicate genes with the non-waxy being dominant to the waxy, the ratio of 63non-waxy : 7waxy in F2:3 and 7non-waxy : 1waxy in BC1F2 were observed. The genes controlling purple grain color are genetically independent to those of waxiness. Five purple-and-waxy wheat lines with homologous waxy and purple genotype were identified in this experiment by selfing purification, and thus a new wheat germplasm, purple-and-waxy wheat, was obtained. 【Conclusion】 Purple grain, having maternal effect, was controlled by two pairs of dominant and complementary genes, waxiness, being recessiveness, was controlled by 3 independent pairs of duplicate genes, and purple-grain wheat, a new germplasm, was obtained.

Key words: wheat, purple grain, waxiness, inheritance, breeding

CLC Number: 

  • S512.1

[1]周文春, 赵寅槐. 糯小麦的遗传、选育和应用前景. 江苏农业学报, 2000, 16(2): 118-123.

Zhou W C, Zhao Y H. Genetics, selection and prospects of waxy wheat. Jiangsu Journal of Agricultural Science, 2000, 16(2): 118-123. (in Chinese)

[2]姚大年, 王新望, 刘志勇, 刘广田. 小麦品种Waxy蛋白的鉴定和筛选. 农业生物技术学报, 1999, 7(1): 1-9.

Yao D N, Wang X W, Liu Z Y, Liu G T. Identification and screen of waxy protein in wheat. Journal of Agricultural Biotechnology, 1999, 7(1): 1-9. (in Chinese)

[3]王子宁, 郭北海, 张艳敏, 温之雨, 李洪杰, 石云素. 小麦地方品种Wx基因构成分析. 华北农学报, 1999, 14(3): 5-9.

Wang Z N, Guo B H, Zhang Y M, Wen Z Y, Li H J, Shi Y S. Discovery and analysis of wheat cultivar (T. aestivum) with Wx genes. Acta Agriculturae Boreali-Sinica, 1999, 14(3): 5-9. (in Chinese)

[4]王子宁, 郭北海, 张艳敏, 温之雨. 小麦糯性突变体的筛选. 华北农学报, 2002, 17(3): 14-19.

Wang Z N, Guo B H, Zhang Y M, Wen Z Y. Mutant screen of wheat cultivars (T.aestivum) for waxy genes (null) types. Acta Agriculturae Boreali-Sinica, 2002, 17(3): 14-19. (in Chinese)

[5]王子宁, 郭北海, 李洪杰, 张艳敏, 温之雨, 李  辉. 小麦(T. aestivum)Waxy-D1基因缺失材料的发现及分析. 作物学报, 2000, 26(3): 257-260.

Wang Z N, Guo B H, Li H J, Zhang Y M, Wen Z Y, Li H. The discovery and analysis of wheat cultivar (T. aestivum) with Waxy-D1 (null) gene. Acta Agronomica Sinica, 2000, 26(3): 257-260. (in Chinese)

[6]Nakamura T, Yamamori M, Hirano H, Hidaka S, Nagamine T. Production of waxy (amylose-free) wheat. Molecular and General Genetics, 1995, 248: 253-259.

[7]Hoshino T, Ito S, Hatta K. Development of waxy common wheat by haploid breeding. Breeding Science, 1996, 46(2): 185-188.

[8]Kiribuchi-Otobe C, Nagamine T, Yanagisawa T, Ohnishi M, Yamaguchi I. Production of hexaploid wheats with waxy endosperm character. Cereal Chemistry, 1997, 74(1): 72-74.

[9]杨学明, 姚金保, 姚国才, 张  勇, 朱新开, 钱存鸣. 糯性普通小麦的选育及其淀粉品质. 江苏农业学报, 2006, 22(1): 91-92.

Yang X M, Yao J B, Yao G C, Zhang Y, Zhu X K, Qian C M. Selection of waxy common wheat and its starch quality. Jiangsu Journal of Agricultural Sciences, 2006, 22(1): 91-92. (in Chinese)

[10]李继刚, 梁荣奇, 张义荣, 李保云，刘广田. 糯性普通小麦的产生及其淀粉特性研究. 麦类作物学报, 2001, 21(2): 10-13.

Li J G, Liang R Q, Zhang Y R, Li B Y, Liu G T. Production of waxy common wheat and its starch properties. Journal of Triticeae Crops, 2001, 21(2): 10-13. (in Chinese)

[11]黄碧光, 吴为人, 刘思衡, 王  煜. 蓝糯小麦种质的选育. 福建农林大学学报: 自然科学版, 2006, 35(5): 456-458.

Huang B G, Wu W R, Liu S H, Wang Y. Breeding and genetic analysis for blue-and-waxy wheat. Journal of Fujian Agriculture and Forestry University: Natural Science Edition, 2006, 35(5): 456-458. (in Chinese)

[12]孙  链, 孙  辉, 姜薇莉, 雷  玲. 糯小麦粉配粉对小麦加工品质的影响: Ⅱ.对面条品质影响的研究. 中国粮油学报, 2010, 25(2): 18-22.

Sun L, Sun H, Jiang W L, Lei L. Flour blending with waxy wheat flour: Ⅱ. Effect on noodle quality. Journal of the Chinese Cereals and Oils Association, 2010, 25(2): 18-22. (in Chinese)

[13]宋建民, 刘爱峰, 尤明山, 李保云, 吴祥云, 赵振东, 刘广田. 糯小麦配粉对淀粉糊化特性和面条品质的影响. 中国农业科学2004, 37(12): 1838-1842.

Song J M, Liu A F, You M S, Li B Y, Wu X Y, Zhao Z D, Liu G T. Effects of waxy flour blending on starch pasting properties and noodle quality of nonwaxy flour. Scientia Agricultura Sinica, 2004, 37(12): 1838-1842. (in Chinese)

[14]梁荣奇, 张义荣, 唐朝晖, 刘守斌, 李保云, 刘广田. 糯性普通小麦的籽粒成分和淀粉品质研究. 中国粮油学报, 2002, 17(4): 12-16.

Liang R Q, Zhang Y R, Tang Z H, Liu S B, Li B Y, Liu G T. Study on the grain composition and starch quality of waxy common wheat. Journal of the Chinese Cereals and Oils Association, 2002, 17(4): 12-16. (in Chinese)

[15]孙  链, 孙  辉, 雷  玲, 姜薇莉. 糯小麦粉配粉理化特性及其对馒头品质的影响. 中国粮油学报, 2009, 24(1): 5-10.

Sun L, Sun H, Lei L, Jiang W L. Flour blending with waxy wheat flour: Physico-chemical properties and effects on steamed bread quality. Journal of the Chinese Cereals and Oils Association, 2009, 24(1): 5-10. (in Chinese)

[16]张  剑, 李梦琴, 上官丽娟. 糯小麦配粉对面粉及馒头品质影响的研究. 河南工业大学报: 自然科学版, 2007, 28(6): 15-18.

Zhang J, Li M Q, Shangguan L J. Effects of waxy wheat flour blending on quality of wheat flour and steamed-bread. Journal of Henan University of Technology: Natural Science Edition, 2007, 28(6): 15-18. (in Chinese)

[17]袁文业, 孙善澄. 紫粒和蓝粒小麦研究综述. 国外农学-麦类作物, 1993(2): 12-14.

Yuan W Y, Sun S D. A review on wheat with blue or purple grains. Journal of Triticeae Crops, 1993(2): 12-14. (in Chinese)

[18]Gilchrist J A, Sorrells M E. Inheritance of kernel colour in ‘charcoal’wheats. Heredity, 1982, 73: 457-460.

[19]Piech J, Evans L E. Monosomic analysis of purple grain color in hexaploid wheat. Zeitschrift fur Pflanzenzüchtg, 1979, 82: 212-217.

[20]李杏普, 侯红军, 刘玉平, 兰素缺, 祝玉英. 蓝、紫粒小麦的营养品质研究. 华北农学报, 2002, 17(1): 21-24.

Li X P, Hou H J, Liu Y P, Lan S Q, Zhu Y Y. Studies of grains nutritional quality on wheat with blue or purple kernels. Acta Agriculturae Boreali-Sinica, 2002, 17(1): 21-24. (in Chinese)

[21]李杏普, 兰素缺, 刘玉平. 蓝、紫粒小麦籽粒色素及其相关生理生化特性的研究. 作物学报, 2003, 29(1): 157-158.

Li X P, Lan S Q, Liu Y P. Studies on pigment and it’s related physio-biochemical properties of blue or purple grain wheat. Acta Agronomica Sinica, 2003, 29(1): 157-158. (in Chinese)

[22]张建军, 李  祥, 侯明生. 小麦品种对梭条斑花叶病毒病抗病特性的研究. 华中农业大学学报, 1996, 15(5): 420-425.

Zhang J J, Li X, Hou M S. The characteristics of wheat varieties resistance to wheat spindle streak mosaic virus (WSSMV). Journal of Huazhong Agricultural University, 1996, 15(5): 420-425. (in Chinese)

[23]郑壮丽, 王成社, 杨进荣, 刘  俊. 黑粒糯小麦材料的鉴定. 麦类作物学报, 2007, 27(3): 433-437.

Zheng Z L, Wang C S, Yang J R, Liu J. Identification of black kernel waxy wheat material, Journal of Triticeae Crops, 2007, 27(3): 433-437. (in Chinese)

[24]邵中子, 邓景杨. 内蒙古紫皮小麦紫皮性状的遗传分析. 遗传学报, 1987, 14(3): 179-187.

Shao Z Z, Deng J Y. Genetic analyses of the purple character in Inner Mongolia purple seed-coat wheat. Acta Genetica Sinica, 1987, 14(3): 179-187. (in Chinese)

[25]徐丙元, 李杏普, 兰素缺, 柏  峰. 微卫星标记定位紫粒小麦色素基因. 河北师范大学学报: 自然科学版, 2007, 31(5): 658.

Xu B Y, Li X P, Lan S Q, Bai F. Location of pigment gene for wheat with purple grains using microsatellite marker. Journal of Hebei Normal University: Natural Science Edition, 2007, 31(5): 658. (in Chinese)

[26]McIntosh R A, Baker E P. Inheritance of purple pericarp in wheat. Proceedings of the Linnaean Society of New South Wales, 1967, 92: 204-208.

[27]Bolton F E. Inheritance of blue aleurone and purple pericarp in hexaploid wheat. Plant Breeding Abstracts, 1968, 40: 2684.

 

[28]孙  群, 孙宝启, 王建华. 黑粒小麦子粒色素的遗传研究. 种子, 2003(4): 33-35.

Sun Q, Sun B Q, Wang J H. Genetic of seed pigment of black kernel wheat. Seed, 2003(4): 33-35. (in Chinese)

[29]李中安, 宁  锟. 糯小麦研究初报. 麦类作物学报, 2001, 21(3): 95-96.

Li Z A, Ning K. Primary report on waxy wheat studies. Journal of Triticeae Crops, 2001, 21(3): 95-96. (in Chinese)

[30]Delwiche S R, Graybosch R A. Identification of waxy wheat by near-infrared reflectance spectroscopy. Journal of Cereal Science, 2002, 35: 29-38.

[31]黄碧光, 范永立, 吴为人. 提高糯性鉴定后小麦种子的成苗率. 福建农林大学学报: 自然科学版, 2006, 35(3): 232-234.

Huang B G, Fan Y L, Wu W R. Increasing the seedling rate of wheat seeds after waxy identification. Journal of Fujian Agriculture and Forestry University: Natural Science Edition, 2006, 35(3): 232-234. (in Chinese)

[32]张伯桥, 吕国峰, 张晓祥, 朱冬梅, 高德荣. Wx基因变异对小麦胚乳质地的影响. 麦类作物学报, 2008, 28(1): 47-50.

Zhang B Q, Lü G F, Zhang X X, Zhu D M, Gao D R. Effects of Wx gene mutation on endosperm traits of wheat. Journal of Triticeae Crops, 2008, 28(1): 47-50. (in Chinese)
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