Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (17): 3501-3507.doi: 10.3864/j.issn.0578-1752.2011.17.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Analysis of Purple and Waxy Grain in Wheat

HUANG  Bi-Guang   

  1. 福建农林大学作物遗传育种与综合利用教育部重点实验室
  • Received:2010-12-30 Revised:2011-03-21 Online:2011-09-01 Published:2011-04-06

Abstract: 【Objective】Waxy-grain wheat and purple-grain wheat are special germplasms with special values in nutrition and quality. Based on the genetic law of waxy and purple grain in concrete materials, purple-and-waxy grain wheat was bred which combined the advantages of both wheats.【Method】Reciprocal cross between waxy-grain wheat C75 and purple-grain wheat 03-Chu-3 was made through the F2:3 generation, and then backcross was done between F1 and C75 through BC1F2 generation. The inheritance of grain color and waxiness were determined according to the phenotypic ratios of each generation, and a new wheat germplasm producing purple-and-waxy grains (named as purple-and-waxy wheat) was created from the filial generation. 【Result】 The purple-grain color, behaving as maternal effect, was controlled by 2 pairs of dominant and complementary genes,and the dosage effect was also found for the purple gene, the ratio of 9purple:7red in F2:3 and 1purple:3red in BC1F2 was observed. The waxiness was controlled by 3 independent pairs of duplicate genes with the non-waxy being dominant to the waxy, the ratio of 63non-waxy : 7waxy in F2:3 and 7non-waxy : 1waxy in BC1F2 were observed. The genes controlling purple grain color are genetically independent to those of waxiness. Five purple-and-waxy wheat lines with homologous waxy and purple genotype were identified in this experiment by selfing purification, and thus a new wheat germplasm, purple-and-waxy wheat, was obtained. 【Conclusion】 Purple grain, having maternal effect, was controlled by two pairs of dominant and complementary genes, waxiness, being recessiveness, was controlled by 3 independent pairs of duplicate genes, and purple-grain wheat, a new germplasm, was obtained.

Key words: wheat, purple grain, waxiness, inheritance, breeding

CLC Number: 

  • S512.1

[1]周文春, 赵寅槐. 糯小麦的遗传、选育和应用前景. 江苏农业学报, 2000, 16(2): 118-123.

Zhou W C, Zhao Y H. Genetics, selection and prospects of waxy wheat. Jiangsu Journal of Agricultural Science, 2000, 16(2): 118-123. (in Chinese)

[2]姚大年, 王新望, 刘志勇, 刘广田. 小麦品种Waxy蛋白的鉴定和筛选. 农业生物技术学报, 1999, 7(1): 1-9.

Yao D N, Wang X W, Liu Z Y, Liu G T. Identification and screen of waxy protein in wheat. Journal of Agricultural Biotechnology, 1999, 7(1): 1-9. (in Chinese)

[3]王子宁, 郭北海, 张艳敏, 温之雨, 李洪杰, 石云素. 小麦地方品种Wx基因构成分析. 华北农学报, 1999, 14(3): 5-9.

Wang Z N, Guo B H, Zhang Y M, Wen Z Y, Li H J, Shi Y S. Discovery and analysis of wheat cultivar (T. aestivum) with Wx genes. Acta Agriculturae Boreali-Sinica, 1999, 14(3): 5-9. (in Chinese)

[4]王子宁, 郭北海, 张艳敏, 温之雨. 小麦糯性突变体的筛选. 华北农学报, 2002, 17(3): 14-19.

Wang Z N, Guo B H, Zhang Y M, Wen Z Y. Mutant screen of wheat cultivars (T.aestivum) for waxy genes (null) types. Acta Agriculturae Boreali-Sinica, 2002, 17(3): 14-19. (in Chinese)

[5]王子宁, 郭北海, 李洪杰, 张艳敏, 温之雨, 李  辉. 小麦(T. aestivum)Waxy-D1基因缺失材料的发现及分析. 作物学报, 2000, 26(3): 257-260.

Wang Z N, Guo B H, Li H J, Zhang Y M, Wen Z Y, Li H. The discovery and analysis of wheat cultivar (T. aestivum) with Waxy-D1 (null) gene. Acta Agronomica Sinica, 2000, 26(3): 257-260. (in Chinese)

[6]Nakamura T, Yamamori M, Hirano H, Hidaka S, Nagamine T. Production of waxy (amylose-free) wheat. Molecular and General Genetics, 1995, 248: 253-259.

[7]Hoshino T, Ito S, Hatta K. Development of waxy common wheat by haploid breeding. Breeding Science, 1996, 46(2): 185-188.

[8]Kiribuchi-Otobe C, Nagamine T, Yanagisawa T, Ohnishi M, Yamaguchi I. Production of hexaploid wheats with waxy endosperm character. Cereal Chemistry, 1997, 74(1): 72-74.

[9]杨学明, 姚金保, 姚国才, 张  勇, 朱新开, 钱存鸣. 糯性普通小麦的选育及其淀粉品质. 江苏农业学报, 2006, 22(1): 91-92.

Yang X M, Yao J B, Yao G C, Zhang Y, Zhu X K, Qian C M. Selection of waxy common wheat and its starch quality. Jiangsu Journal of Agricultural Sciences, 2006, 22(1): 91-92. (in Chinese)

[10]李继刚, 梁荣奇, 张义荣, 李保云,刘广田. 糯性普通小麦的产生及其淀粉特性研究. 麦类作物学报, 2001, 21(2): 10-13.

Li J G, Liang R Q, Zhang Y R, Li B Y, Liu G T. Production of waxy common wheat and its starch properties. Journal of Triticeae Crops, 2001, 21(2): 10-13. (in Chinese)

[11]黄碧光, 吴为人, 刘思衡, 王  煜. 蓝糯小麦种质的选育. 福建农林大学学报: 自然科学版, 2006, 35(5): 456-458.

Huang B G, Wu W R, Liu S H, Wang Y. Breeding and genetic analysis for blue-and-waxy wheat. Journal of Fujian Agriculture and Forestry University: Natural Science Edition, 2006, 35(5): 456-458. (in Chinese)

[12]孙  链, 孙  辉, 姜薇莉, 雷  玲. 糯小麦粉配粉对小麦加工品质的影响: Ⅱ.对面条品质影响的研究. 中国粮油学报, 2010, 25(2): 18-22.

Sun L, Sun H, Jiang W L, Lei L. Flour blending with waxy wheat flour: Ⅱ. Effect on noodle quality. Journal of the Chinese Cereals and Oils Association, 2010, 25(2): 18-22. (in Chinese)

[13]宋建民, 刘爱峰, 尤明山, 李保云, 吴祥云, 赵振东, 刘广田. 糯小麦配粉对淀粉糊化特性和面条品质的影响. 中国农业科学2004, 37(12): 1838-1842.

Song J M, Liu A F, You M S, Li B Y, Wu X Y, Zhao Z D, Liu G T. Effects of waxy flour blending on starch pasting properties and noodle quality of nonwaxy flour. Scientia Agricultura Sinica, 2004, 37(12): 1838-1842. (in Chinese)

[14]梁荣奇, 张义荣, 唐朝晖, 刘守斌, 李保云, 刘广田. 糯性普通小麦的籽粒成分和淀粉品质研究. 中国粮油学报, 2002, 17(4): 12-16.

Liang R Q, Zhang Y R, Tang Z H, Liu S B, Li B Y, Liu G T. Study on the grain composition and starch quality of waxy common wheat. Journal of the Chinese Cereals and Oils Association, 2002, 17(4): 12-16. (in Chinese)

[15]孙  链, 孙  辉, 雷  玲, 姜薇莉. 糯小麦粉配粉理化特性及其对馒头品质的影响. 中国粮油学报, 2009, 24(1): 5-10.

Sun L, Sun H, Lei L, Jiang W L. Flour blending with waxy wheat flour: Physico-chemical properties and effects on steamed bread quality. Journal of the Chinese Cereals and Oils Association, 2009, 24(1): 5-10. (in Chinese)

[16]张  剑, 李梦琴, 上官丽娟. 糯小麦配粉对面粉及馒头品质影响的研究. 河南工业大学报: 自然科学版, 2007, 28(6): 15-18.

Zhang J, Li M Q, Shangguan L J. Effects of waxy wheat flour blending on quality of wheat flour and steamed-bread. Journal of Henan University of Technology: Natural Science Edition, 2007, 28(6): 15-18. (in Chinese)

[17]袁文业, 孙善澄. 紫粒和蓝粒小麦研究综述. 国外农学-麦类作物, 1993(2): 12-14.

Yuan W Y, Sun S D. A review on wheat with blue or purple grains. Journal of Triticeae Crops, 1993(2): 12-14. (in Chinese)

[18]Gilchrist J A, Sorrells M E. Inheritance of kernel colour in ‘charcoal’wheats. Heredity, 1982, 73: 457-460.

[19]Piech J, Evans L E. Monosomic analysis of purple grain color in hexaploid wheat. Zeitschrift fur Pflanzenzüchtg, 1979, 82: 212-217.

[20]李杏普, 侯红军, 刘玉平, 兰素缺, 祝玉英. 蓝、紫粒小麦的营养品质研究. 华北农学报, 2002, 17(1): 21-24.

Li X P, Hou H J, Liu Y P, Lan S Q, Zhu Y Y. Studies of grains nutritional quality on wheat with blue or purple kernels. Acta Agriculturae Boreali-Sinica, 2002, 17(1): 21-24. (in Chinese)

[21]李杏普, 兰素缺, 刘玉平. 蓝、紫粒小麦籽粒色素及其相关生理生化特性的研究. 作物学报, 2003, 29(1): 157-158.

Li X P, Lan S Q, Liu Y P. Studies on pigment and it’s related physio-biochemical properties of blue or purple grain wheat. Acta Agronomica Sinica, 2003, 29(1): 157-158. (in Chinese)

[22]张建军, 李  祥, 侯明生. 小麦品种对梭条斑花叶病毒病抗病特性的研究. 华中农业大学学报, 1996, 15(5): 420-425.

Zhang J J, Li X, Hou M S. The characteristics of wheat varieties resistance to wheat spindle streak mosaic virus (WSSMV). Journal of Huazhong Agricultural University, 1996, 15(5): 420-425. (in Chinese)

[23]郑壮丽, 王成社, 杨进荣, 刘  俊. 黑粒糯小麦材料的鉴定. 麦类作物学报, 2007, 27(3): 433-437.

Zheng Z L, Wang C S, Yang J R, Liu J. Identification of black kernel waxy wheat material, Journal of Triticeae Crops, 2007, 27(3): 433-437. (in Chinese)

[24]邵中子, 邓景杨. 内蒙古紫皮小麦紫皮性状的遗传分析. 遗传学报, 1987, 14(3): 179-187.

Shao Z Z, Deng J Y. Genetic analyses of the purple character in Inner Mongolia purple seed-coat wheat. Acta Genetica Sinica, 1987, 14(3): 179-187. (in Chinese)

[25]徐丙元, 李杏普, 兰素缺, 柏  峰. 微卫星标记定位紫粒小麦色素基因. 河北师范大学学报: 自然科学版, 2007, 31(5): 658.

Xu B Y, Li X P, Lan S Q, Bai F. Location of pigment gene for wheat with purple grains using microsatellite marker. Journal of Hebei Normal University: Natural Science Edition, 2007, 31(5): 658. (in Chinese)

[26]McIntosh R A, Baker E P. Inheritance of purple pericarp in wheat. Proceedings of the Linnaean Society of New South Wales, 1967, 92: 204-208.

[27]Bolton F E. Inheritance of blue aleurone and purple pericarp in hexaploid wheat. Plant Breeding Abstracts, 1968, 40: 2684.

 

[28]孙  群, 孙宝启, 王建华. 黑粒小麦子粒色素的遗传研究. 种子, 2003(4): 33-35.

Sun Q, Sun B Q, Wang J H. Genetic of seed pigment of black kernel wheat. Seed, 2003(4): 33-35. (in Chinese)

[29]李中安, 宁  锟. 糯小麦研究初报. 麦类作物学报, 2001, 21(3): 95-96.

Li Z A, Ning K. Primary report on waxy wheat studies. Journal of Triticeae Crops, 2001, 21(3): 95-96. (in Chinese)

[30]Delwiche S R, Graybosch R A. Identification of waxy wheat by near-infrared reflectance spectroscopy. Journal of Cereal Science, 2002, 35: 29-38.

[31]黄碧光, 范永立, 吴为人. 提高糯性鉴定后小麦种子的成苗率. 福建农林大学学报: 自然科学版, 2006, 35(3): 232-234.

Huang B G, Fan Y L, Wu W R. Increasing the seedling rate of wheat seeds after waxy identification. Journal of Fujian Agriculture and Forestry University: Natural Science Edition, 2006, 35(3): 232-234. (in Chinese)

[32]张伯桥, 吕国峰, 张晓祥, 朱冬梅, 高德荣. Wx基因变异对小麦胚乳质地的影响. 麦类作物学报, 2008, 28(1): 47-50.

Zhang B Q, Lü G F, Zhang X X, Zhu D M, Gao D R. Effects of Wx gene mutation on endosperm traits of wheat. Journal of Triticeae Crops, 2008, 28(1): 47-50. (in Chinese)
[1] ZHU Qi, JIA ZhenPeng, Tahir SHAH, XU ChenSheng, LI ZhiQi, LÜ HuiShuai, ZHU PengChao, WEI XiaoMin, HUANG DongLin, SUN YanNi, CAO WeiDong, GAO YaJun, WANG ZhaoHui, ZHANG DaBin. Green Manure Crops Combined with Enhanced-Efficiency Products Reduced Greenhouse Gas Emissions and Carbon Footprints in Dryland Wheat Fields [J]. Scientia Agricultura Sinica, 2026, 59(7): 1507-1522.
[2] LI WenHu, LI HaiFeng, DU YuPeng, DING YuLan, LUO YiNuo, LI YuKe, SHE WenTing, ZHANG Feng, TENG Yu, ZHANG SiQi, HUANG Cui, LI XiaoHan, LIU JinShan, WANG ZhaoHui. Regional Differences in Wheat Zinc Uptake and Translocation Responses to Soil Zinc Fertilization [J]. Scientia Agricultura Sinica, 2026, 59(5): 1034-1047.
[3] MA ZhaoHui, QUAN ChengZhe, CHENG HaiTao, YANG KanJie, LI XinRui, LÜ WenYan. The Breeding Goals and Strategies of Northeast Japonica Rice Under the Background of Zhongke Fa No.5 [J]. Scientia Agricultura Sinica, 2026, 59(5): 927-936.
[4] JIAO WenJuan, HE WanLong, GENG HongWei, BAI Bin, LI JianFeng, CHENG YuKun. Stripe Rust Resistance Evaluation and Molecular Characterization of Yr Genes for 155 Spring Wheat Varieties (Lines) [J]. Scientia Agricultura Sinica, 2026, 59(5): 937-950.
[5] ZHANG WenXuan, XIE ShuoQi, WU Xin, WANG YueQiang, LI YangGuang, ZHANG Zhen, REN XiaoLi, GAO TengYun, LIANG Dong, HUANG HeTian. Estimation of Genetic Parameters and Breeding Values for Birth Weight and Weaning Weight in Chinese Holstein Cattle [J]. Scientia Agricultura Sinica, 2026, 59(4): 900-911.
[6] CUI ShiYou, CHEN PengJun, MIAO YuanQing, HAN JiJun, SHEN JunMing. Development and Field Evaluation of Glyphosate-Resistant Wheat Germplasm Generated Through EMS Mutagenesis [J]. Scientia Agricultura Sinica, 2026, 59(4): 723-733.
[7] QIAN Jin, LI YingXue, WU Fang, ZOU XiaoChen. Improved Leaf Phosphorus Content Estimation of Winter Wheat Using Ensemble Hyperspectral Dimensionality Reduction Method [J]. Scientia Agricultura Sinica, 2026, 59(4): 781-792.
[8] KONG Yuan, CUI ShaSha, LI Mei, LI Jian, YANG SiYu, FANG Feng, LIU ShuaiShuai, LIU MingPing, ZENG Yan, GAO XingXiang, BAI LianYang. Spatiotemporal Distribution Dynamics of Five Grass Weed Species Including Lolium multiflorum in Winter Wheat Fields of the Huang- Huai-Hai Region [J]. Scientia Agricultura Sinica, 2026, 59(4): 807-823.
[9] WANG YongSheng, NIU Li, WANG ChangJie, MA LiHua, LIAN XiaoXiao, MENG YaXiong, MA XiaoLe, YAO LiRong, ZHANG Hong, YANG Ke, LI BaoChun, WANG HuaJun, SI ErJing, WANG JunCheng. Genome-Wide Association Study and Candidate Gene Identification for Thousand Grain Weight in Winter Wheat [J]. Scientia Agricultura Sinica, 2026, 59(3): 499-514.
[10] LI XinYi, LI JiaNing, YANG WenPing, XIA Qing, HUO YingRui, HAO ShiHang, HUANG TingMiao, REN YongKang, CHEN Jie, GAO ZhiQiang, YANG ZhenPing. Effects of Post-Anthesis Foliar Zinc Application on Zinc Nutrition in Colored-Grain Wheat [J]. Scientia Agricultura Sinica, 2026, 59(3): 515-527.
[11] XIAN QingLin, XIAO JianKe, GAO AQing, GAO LiChuang, LIU Yang. Effects of Planting Patterns Combined with Soil Moisture Measurement and Supplementary Irrigation on the Yield and Water Use Efficiency of Winter Wheat [J]. Scientia Agricultura Sinica, 2026, 59(3): 589-601.
[12] LÜ WenYan, CHENG HaiTao, MA ZhaoHui, TIAN ShuHua. Discussion on Hybridization Breeding Technology and Strategy of Rice in the New Era of Breeding [J]. Scientia Agricultura Sinica, 2026, 59(2): 233-238.
[13] ZHANG ZhiYong, TAN ShiChao, XIONG ShuPing, MA XinMing, WEI YiHao, WANG XiaoChun. Effects of Annual Water and Nitrogen Optimization on Yield and Nitrogen Migration of Wheat-Maize Rotation System in Irrigation Area of Northern Henan [J]. Scientia Agricultura Sinica, 2026, 59(2): 336-353.
[14] LÜ XuDong, SUN ShiYuan, LI YaNan, LIU YuLong, WANG YanQun, FU Xin, ZHANG JiaYing, NING Peng, PENG ZhengPing. Effects of Intelligent Mechanized Layered Fertilization on Root-Soil Nutrient Distribution and Yield in Wheat Fields [J]. Scientia Agricultura Sinica, 2026, 59(1): 129-146.
[15] LU Hao, ZHANG MingLong, HAN Mei, YAN QingBiao, LI ZhengPeng, YIN Wen, FAN ZhiLong, HU FaLong, CHAI Qiang. Green Manure Returning via Sheep Digest with Nitrogen Fertilizer Reduction are Beneficial to Improve Wheat Yield and Soil Quality at Qinghai-Tibet Plateau [J]. Scientia Agricultura Sinica, 2026, 59(1): 147-160.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!