转反义GhADF1基因对陆地棉纤维品质的影响

doi:10.3864/j.issn.0578-1752.2009.12.003

Scientia Agricultura Sinica ›› 2009, Vol. 42 ›› Issue (12): 4139-4144 .doi: 10.3864/j.issn.0578-1752.2009.12.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles Next Articles

Effects of Transforming Antisense GhADF1 Gene on the Fiber Quality in Upland Cotton (Gossypium hirsutum L.)

NAN Zhi-run, LI Hong-fang, ZHANG Huan-yang, LI Jing, LI Jun-feng, WANG Jiao-juan, JIAO Gai-li

（山西省农业科学院棉花研究所）

Received:2008-11-12 Revised:2009-08-05 Online:2009-12-10 Published:2009-12-10
Contact: JIAO Gai-li

Abstract

Abstract:

【Objective】 To detect the effects of suppressing actin-depolymerizing factor gene of G. hirsutum L. (GhADF1), antisense GhADF1 gene was introduced into upland cotton. 【Method】 The ORF of GhADF1 was inserted into plant expression vector pPZP111 in the anti-sense orientation. The recombinant plasmid was introduced into cotton Coker312 (G. hirsutum L.) by the method of agrobacterium-mediated transformation. 【Result】 PCR and Southern blot showed that the antisense GhADF1 gene was integrated into the cotton genome and could be inherited stably. The fiber quality traits analysis indicated that the fiber length and strength of 3 pure transgenic lines were significantly improved compared with that of Coker312. 【Conclusion】 Transformation of antisense GhADF1 gene could improve fiber strength and cotton fiber quality.

Key words: actin-depolymerizing factor gene of G. hirsutum L. (GhADF1), transgenic cotton, genome, fiber quality analysis

NAN Zhi-run,LI Hong-fang,ZHANG Huan-yang,LI Jing,LI Jun-feng,WANG Jiao-juan,JIAO Gai-li
. Effects of Transforming Antisense GhADF1 Gene on the Fiber Quality in Upland Cotton (Gossypium hirsutum L.)
[J].Scientia Agricultura Sinica, 2009, 42(12): 4139-4144 .

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