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Table of Content

    10 December 2009, Volume 42 Issue 12
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
    The Innovation of Dwarf Male Sterile Wheat and Its Application in Wheat Breading
    ZHAI Hu-qu,LIU Bing-hua
    Scientia Agricultura Sinica. 2009, 42(12):  4127-4131 .  doi:10.3864/j.issn.0578-1752.2009.12.001
    Abstract ( 1010 )   PDF (190KB) ( 1027 )   Save
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    To illustrate the discovery, identification of Taigu male sterile wheat were illustrated, and the gene was located in 4DS. It was found that it is a dominant gene and named Ms2. Looking for restorer line for Ms2 was made, but it is difficult. The dwarf gene of wheat was reported in 4DS of wheat, are the dwarf gene and Ms2 linkage? After test, the results indicated that they are closely linked, so, Liu (1986) created a dwarf male sterile wheat. It provided a platform for wheat breeding. According to the platform in recent decades, new varieties were bred. These varieties were grown in large scale (more than ten million hectare), but all the varieties are pure inbreed lines. Can the platform be used in hybrid breeding? The answer is definite. We should find a mark gene which would be closely linked to dwarf gene in the future study.
    Cloning of Full-Length cDNAs Encoding Two Methyl-Binding Domain Proteins and Their Expression Patterns in Wheat Seeds
    MENG Fan-rong,LI Yong-chun,LING Na,WANG Xiao,SI Zhi-fei,ZHANG Yan-xia,YIN Jun
    Scientia Agricultura Sinica. 2009, 42(12):  4132-4138 .  doi:10.3864/j.issn.0578-1752.2009.12.002
    Abstract ( 1184 )   PDF (643KB) ( 969 )   Save
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    【Objective】 Analyzing the sequence characteristics of two wheat MBD genes, TaMBD1 and TaMBD6 and their expression patterns during the seed development and germination, which will provide an insight into epigenetic regulation mechanisms involved in the development and germination of wheat seeds. 【Method】 RACE technology was used in full-length cDNAs cloning and proper bioinformatics softwares were applied for characterizing the cloned genes and the deduced proteins. Semi-quantitative RT-PCR was performed to detect the expression patterns of cloned genes. 【Result】 Two full-length cDNAs of TaMBD1 and TaMBD6 were cloned, which encodes 193 and 187 amino acids, respectively. The amino acid sequence analysis showed that a typical methyl-CpG-binding domain was included in TaMBD1 and TaMBD6, and a CW-type Zinc finger domain was found in TaMBD1, additionally. Structure prediction revealed that both of TaMBD1 and TaMBD6 could fold into an alpha/beta sandwich structure comprising a layer of twisted beta sheet, backed by another layer formed by the alpha1 helix and loops at the C terminus. The RT-PCR analysis showed that the TaMBD1 was steadily expressed with a relative higher level during the seed development, while gradually up- and down- regulated in embryo and endosperm tissues, respectively, along with the germination of wheat seeds. The expression of TaMBD6 is vibrated during the seed development and it was highly expressed at the time point of 15 days after pollination. However, the expression of TaMBD6 was not detected during the seed germination. 【Conclusion】 Two full-length cDNAs of TaMBD1 and TaMBD6 were firstly cloned from wheat and the typical DNA binding domains were found in the deduced proteins. The expression patterns of these two genes indicated that TaMBD1 might play an importan regulating role during the seed development and germination in wheat, while TaMBD6 was only involved in the regulation of seed development. The results provided important information for further studies on molecular regulation mechanism during the seed development and germination in wheat.

    Effects of Transforming Antisense GhADF1 Gene on the Fiber Quality in Upland Cotton (Gossypium hirsutum L.)
    NAN Zhi-run,LI Hong-fang,ZHANG Huan-yang,LI Jing,LI Jun-feng,WANG Jiao-juan,JIAO Gai-li
    Scientia Agricultura Sinica. 2009, 42(12):  4139-4144 .  doi:10.3864/j.issn.0578-1752.2009.12.003
    Abstract ( 949 )   PDF (433KB) ( 787 )   Save
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    【Objective】 To detect the effects of suppressing actin-depolymerizing factor gene of G. hirsutum L. (GhADF1), antisense GhADF1 gene was introduced into upland cotton. 【Method】 The ORF of GhADF1 was inserted into plant expression vector pPZP111 in the anti-sense orientation. The recombinant plasmid was introduced into cotton Coker312 (G. hirsutum L.) by the method of agrobacterium-mediated transformation. 【Result】 PCR and Southern blot showed that the antisense GhADF1 gene was integrated into the cotton genome and could be inherited stably. The fiber quality traits analysis indicated that the fiber length and strength of 3 pure transgenic lines were significantly improved compared with that of Coker312. 【Conclusion】 Transformation of antisense GhADF1 gene could improve fiber strength and cotton fiber quality.

    QTL Mapping for Fiber Quality Properties in Upland Cotton (Gossypium hirsutum L.)
    QIN Yong-sheng,YE Wen-xue,LIU Ren-zhong,ZHANG Tian-zhen,GUO Wang-zhen
    Scientia Agricultura Sinica. 2009, 42(12):  4145-4154 .  doi:10.3864/j.issn.0578-1752.2009.12.004
    Abstract ( 990 )   PDF (529KB) ( 952 )   Save
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    【Objective】 In this paper, two F2 mapping populations were respectively assembled by using the parents of CRI28 hybrid (CRI12 and 4133, their corresponding population named as Pop1) and the parents of XZM 2 hybrid (CRI12 and 8891, their corresponding population named as Pop2), to construct linkage map with higher genome coverage, to map QTLs related with fiber qualities, and further to provide information for molecular marker-assisted breeding. 【Method】 The linkage map was constructed with SSR markers by JOINMAP 3.0 software. The QTLs for 6 fiber quality properties in F2 and F2:3 populations were mapped by the composite interval mapping method (CIM) using Win QTLCart 2.5. 【Result】 A joinmap was integrated with 245 loci and covered 1 847.81 cM. Based on this, QTLs related with fiber qualities were further detected. In detail, 39 QTLs were identified in three environments in separate analysis and 22 QTLs in the joint analysis in Pop1. Similarly, 51 and 18 QTLs were identified in Pop2, respectively. QTLs were clustered on chromosomes A3, D2 and D9, and some stably heritable QTLs were also discovered by integrating analysis in different environments. Of them, 8 QTLs for the 4 traits could be found simultaneously in the two populations, and more 4 QTLs for fiber strength, micronaire, and fiber elongation were offered by CRI12. 【Conclusion】 These stably heritable QTLs could be used for the molecular breeding of fiber quality traits in upland cotton.

    Detection of QTLs of Yield Related Traits in Soybean
    HUANG Zhong-wen,ZHAO Tuan-jie,YU De-yue,CHEN Shou-yi,GAI Jun-yi
    Scientia Agricultura Sinica. 2009, 42(12):  4155-4165 .  doi:10.3864/j.issn.0578-1752.2009.12.005
    Abstract ( 911 )   PDF (271KB) ( 1193 )   Save
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    【Objective】 The present study was aimed to reveal the correlation between yield and biomass, leaf area and yield components, and to detect and map the QTLs of the yield-related traits. 【Method】 The 184 recombinant inbred lines derived from a cross between geographically and genetically distant parents, Kefeng 1 from northern China and Nannong 1138-2 from southern China, were tested in two years and then analyzed for detecting and mapping QTLs of the traits related to yield. 【Result】 A very significant positive correlation was observed between yield and above ground biomass, leaf area index, root weight, canopy width and canopy height with r values of 0.5-0.7. Seven QTLs of above ground biomass were mapped with contribution rate of 6.2%-21.1% and one QTL (qSBO-1) repeatedly detected in both years while eight QTLs of root weight mapped with contribution rate of 5.2%-20.1% and one QTL (qRTB1-1) repeatedly detected. Five QTLs of R1 leaf area index were mapped with contribution rate of 6.4%-17.2% while five QTLs of R3 leaf area index mapped with contribution rate of 7.3%-26.2% and one QTL (qLAIR3A2) repeatedly detected. Four QTLs of canopy width were mapped with contribution rate of 6.3%-13.1% and one QTL (qCWD1b-2) repeatedly detected while 11 QTLs of canopy height mapped with contribution rate of 5.2%-9.2% and four QTLs repeatedly detected. Six, two and one QTL of 100-seed weight, number of seeds per pod and number of pods per plant, respectively, were mapped with their contribution rate of 6.9%-15.7%. Five and three QTLs of number of pods on branches and number of pods on main stem, respectively, were mapped with their contribution rate of 6.3%-11.1%. Eight and three QTLs of number of nodes on main stem and effective number of branches, respectively, were mapped with their contribution rate of 4.7%-15.2%. There was one QTL shared by above ground biomass and root weight, two QTLs shared by R1 and R3 leaf area index, two QTLs by canopy width and height, one by yield and number of pods per plant, one by 100-seed weight and number of pods on branches, one by number of seeds per pod and number of nodes on main stem, and one by number of pods on branches and effective number of branches. 【Conclusion】 A total of 68 QTLs were mapped for 13 yield-related traits. Among them, only a small part was repeatedly detected in both years, implied their expression depended on years or environmental conditions. Although significant correlations existed among the traits extensively, only a small part of the QTLs was shared between the traits, which indicted different gene systems for various traits. There were few QTLs with large contribution found in the yield-related traits, thus breeding technology for pyramiding multiple genes should be considered for yield improvement.

    Genetic Diversity in Tartary Buckwheat Revealed by AFLP Analysis
    HOU Ya-jun,ZHANG Zong-wen,WU Bin,LI Yan-qin
    Scientia Agricultura Sinica. 2009, 42(12):  4166-4174 .  doi:10.3864/j.issn.0578-1752.2009.12.006
    Abstract ( 900 )   PDF (502KB) ( 1103 )   Save
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    【Objective】 The genetic diversity of tartary buckwheat accessions was analyzed at molecular level, and the helpful information for breeding and germplasm evaluation was obtained. 【Method】 The genetic diversity of 165 accessions of tartary buckwheat from 14 different geographical regions was analyzed using 20 informative primer pairs of AFLP markers. 【Result】 Totally 938 loci were detected among which 314 (33.48%) were polymorphic. The number of amplified fragments and polymorphic fragments per primer combination were 46.9 and 15.7, respectively. Shannon’s information index of different geographical groups was 0.1093-0.2661. The group from Sichuan possessed the highest level of genetic diversity, followed by those from Qinghai, Yunnan and Gansu/Ningxia. The group from Hunan was the lowest in genetic diversity. Five cluster groups were identified based on the dendrogram of pairwise Nei’s genetic identity. The clustering results revealed that the genetic diversity of accessions of tartary buckwheat closely related to their origins. Five types of population structure within 165 tartary buckwheat accessions were inferred by structure analysis, which also correlated to their geographic origins. The population structure of accessions from Yunnan and Sichuan provinces were the most complicated and multiplex. 【Conclusion】 The result showed that the genetic relationship and diversity of tartary buckwheat populations correlated to their geographic distribution to a certain extent.

    Activation of Reactive Oxygen Species and Defense Responses in Tobacco Cells Treated with Riboflavin

    LIU Fei,WEI Fang-fang,WANG Lei,LIU Hui,LIANG Yuan-cun,LIU Ai-xin
    Scientia Agricultura Sinica. 2009, 42(12):  4175-4175 .  doi:10.3864/j.issn.0578-1752.2009.12.007
    Abstract ( 947 )   PDF (461KB) ( 1012 )   Save
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    【Objective】 To study the triggering defense responses in tobacco suspension cells after treated with riboflavin, reactive oxygen species (ROS) and defense-related responses were investigated together with their related effects involving in calcium signals and protein kinases. 【Method】 Tobacco suspension cells were treated with 1 mmol•L-1 riboflavin or pretreated with inhibitors including calcium surrogate La3+, calcium chelator EGTA and staurosporine which is an inhibitor of serine/threonine protein kinase. A series of dynamic defense responses were then studied with several methods, including semiquantitative RT-PCR (reverse transcriptase-polymerase chain reaction) and HPLC (high performance liquid chromatography) techniques. 【Result】 It was identified that a series of defense-related responses were induced in tobacco suspension cells after treated with riboflavin, such as an oxidative burst, resulting in alkalization of the extracellular medium, activated the expression of four defense-related genes, and the scopoletin accumulation. And all the tested three inhibitors were shown in suppressing the riboflavin triggered defense responses at difference levels. 【Conclusion】 These results suggest that riboflavin triggers plant basal defense signal pathway which are also concerned with calcium signal pathway and protein phosphorylation in tobacco cell suspensions as an elicitor.

    Expression of Flavonoid 3′-hydrolyase from Eupatorium adenophorum in Tobacco
    ZHANG Song-huan,GUO Hui-ming,PEI Xi-xiang,LI Chun-qi,CHENG Hong-mei
    Scientia Agricultura Sinica. 2009, 42(12):  4182-4186 .  doi:10.3864/j.issn.0578-1752.2009.12.008
    Abstract ( 903 )   PDF (431KB) ( 655 )   Save
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    【Objective】 The objective of this study is to identify the function of F3′H gene from Eupatorium adenophorum in the secondary metabolism pathway. 【Method】 The over-expression vector of F3′H gene was constructed, and transformed into tobacco, furthermore, semi-quantitative PCR and real-time PCR were used in the study to detect the status of F3′H gene.【Result】 Although F3′H gene could be expressed in the transgenic tobacco normally, it was suppressed after flowering. The color of flowers became less bright in transgenic tobaccos than in wild-type tobaccos, at the same time, the level of F3′H gene was lower in transgenic tobacco than in wild-type tobaccos. 【Conclusion】 The result suggested that F3′H gene has close relation with the formation of anthocyanins in the secondary metabolism pathway. Expression of F3′H gene affected with endogenous F3′H gene each other after it was transferred into tobaccos, which lead to slight loss of color in flowers.

    TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY
    Difference of Source-Sink Components in Conventional Indica Rice Cultivars with Different Panicle Weight
    DONG Gui-chun,JU Jing,LI Jin-qian,YU Xiao-feng,TIAN Hao,ZHANG Biao,ZHOU Juan,WANG Yu-long
    Scientia Agricultura Sinica. 2009, 42(12):  4187-4196 .  doi:10.3864/j.issn.0578-1752.2009.12.009
    Abstract ( 1053 )   PDF (285KB) ( 795 )   Save
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    【Objective】 This study was aimed to analyze the difference of source-sink components in conventional indica rice cultivars with different panicle weights(PW)and to provide valuable information for the cultivation and genetic improvements of lager PW cultivars. 【Method】 Eighty-eight and 122 conventional indica rice cultivars were solution-cultured in 2001 and 2002, respectively. Leaf area index (LAI),dry matter weight (including root, culm and sheath, leaves, panicle), nitrogen content in different organs, yield and its components were measured. Based on PW, these cultivars were classified into 6 types from low to high by the MinSSw method, to study their difference in source and sink components. 【Result】 LAI and green leaf weight at heading and maturity stages, leaf weight per unit leaf area in large PW types of indica rice were superior to those of cultivars with small PW type, however, decline rate of LAI during grain filling period was lower in large-PW cultivar. Elevation LAI could improve PW; Cultivars with large PW showed higher net assimilation rate during grain filling period, and sink potential, grain yield per unit leaf area, sink potential per unit leaf area, sink potential per unit dry matter weight and sink potential per unit N accumulation at heading stage were also higher in cultivars with large PW than those of cultivars with small PW type, enhancing PW could improve sink potential, PW was significantly influenced by green leaf weight at heading stage, net assimilation rate, sink potential per unit area, sink potential per unit leaf area, sink potential per unit N accumulation and grain yield per unit leaf area. 【Conclusion】 LAI, sink and their respective components in large PW types of indica rice were superior to those of cultivars with small PW type. PW was significantly influenced by source traits including green leaf weight at heading stage, net assimilation rate, and sink traits including sink potential per unit area, sink potential per unit leaf area, sink potential per unit N accumulation, grain yield per unit leaf area.

    Research Dynamics on Super Rice Based on Bibliometric
    LI Xiao,CHEN Chun-yan,ZHENG Jia-kui,TANG Sha
    Scientia Agricultura Sinica. 2009, 42(12):  4197-4208 .  doi:10.3864/j.issn.0578-1752.2009.12.010
    Abstract ( 1127 )   PDF (301KB) ( 1102 )   Save
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    【Objective】 Analyze the current situation of super rice researches both in China and in the world and provide data reference for super rice researchers and deciders. 【Method】 Choose web of science, CABI and CNKI databases to analyze the amount of super rice published articles and citation of research countries, authors, institutions, journals and research content. 【Result】 Searched 271 articles on 102 journals by 215 authors of 143 institutes in 21 countries, such as China, Japan, and Philippines from Web of science and CABI, and 1321 articles on 182 journals by 954 authors of 715 institutes in China from CNKI. The research content for super rice involves cultivation, genetic and breeding, and biochemistry, etc. 【Conclusion】 The super rice research in China is in the leading place in the world. Research institutions are widely distributed, research authors are plenty and the research content is plentiful. However, the team of authors who have published a great number of papers of super rice is not strong enough, the number of core authors is small and the quality of papers is low, the institutions which can publish plenty super rice papers are not enough and the number of papers in different research directions is very uneven.

    Dynamic Changes of Ca2+ and Ca2+-ATPase in Sieve Elements in the Developing Caryopsis of Triticum aestivum L.
    LI Ji-wei,DENG Xiang-yi,ZHOU Zhu-qing,WANG Li-kai,YANG Chao-nan,FAN Hai-yan
    Scientia Agricultura Sinica. 2009, 42(12):  4209-4217 .  doi:10.3864/j.issn.0578-1752.2009.12.011
    Abstract ( 995 )   PDF (1912KB) ( 637 )   Save
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    【Objective】 Previous study revealed that sieve elements (SEs) in the developing caryopsis of Triticum aestivum L. underwent a unique type of programmed cell death (PCD) . In this paper, the dynamic changes and the roles of Ca2+ and Ca2+-ATPase in SEs during the PCD were studied. 【Method】 The ultrastructural aspects of phloem cells in wheat caryopsis were examined by transmission electron microscopy (TEM). Using specific fluorescence staining and potassium pyroantimonate precipitation method, Ca2+ was localized at histological and sub-cellular levels in SEs in the developing wheat caryopsis. TEM and lead nitrate were used to locate Ca2+-ATPase in SEs. 【Result】 TEM studies showed that the cell walls of SEs thickened at the beginning of differentiation, and then became thinner and smoother. Fluorescence staining showed that the fluorescence due to Ca2+ appeared in cell walls of SEs from 6 to 10 d after flowering. The fluorescence due to Ca2+ in cell walls of SEs was most notable on 9 d after flowering and disappeared on 14 d after flowering. Sub-celluar localization of Ca2+ showed that Ca2+ was localized on plasma membrane and in nuclei from 1 to 2 d after flowering. On 4 d after flowering, Ca2+ was localized in cytoplasm and mitochondria of SEs. From 5 to 8 d after flowering, Ca2+ was transported to the cell walls of SEs and no Ca2+ precipitates were observed in mitochondria. From 10 to 18 d after flowering, Ca2+ was transported into the cytoplasm again from cell walls and no Ca2+ precipitates were observed on 20 d after flowering. In intermediary cells (ICs), Ca2+ precipitates were observed from 1 to 18 d after flowering, and Ca2+ mainly distributed on intine and tonoplast. The activity of Ca2+-ATPase changed obviously during the SEs differentiation. There was lowest activity of Ca2+-ATPase on 3 d after flowering in SEs. High levels of Ca2+-ATPase activity were found from 4 to 14 d after flowering in SEs, and the enzyme was mainly localized in cell walls, cytoplasm, plasmodesmata, mitochondria and nuclei. 【Conclusion】 These results showed the dynamic changes of Ca2+ and Ca2+-ATPase in SEs differentiation. Ca2+ might play important roles in SEs during the PCD. In addition, Ca2+ and Ca2+-ATPase might be also related to cell wall thickening and functions of SEs.

    Effects of Different Irrigation Treatments on Water Consumption Characteristics and Grain Starch Components Accumulation in Strong Gluten Wheat Jimai 20
    ZHANG Yong-li,YU Zhen-wen,ZHENG Cheng-yan,GU Shu-bo
    Scientia Agricultura Sinica. 2009, 42(12):  4218-4227 .  doi:10.3864/j.issn.0578-1752.2009.12.012
    Abstract ( 965 )   PDF (272KB) ( 669 )   Save
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    【Objective】 The objective of this experiment was to study the effect of different irrigation treatments on characteristics of water consumption, grain starch components accumulation, grain weight and yield in wheat under different soil texture, so as to provide a theoretical basis for water-saving and high-yield cultivation of wheat. 【Method】 The research was carried out with strong gluten wheat cultivar Jimai 20 by setting different irrigation times and amounts in wheat growth seasons in 2004-2005 and 2006-2007, Water balance measurement was used to calculate water consumption amount during the wheat growth period, and Dual-Wavelength Spectrophotometry was used to determine the amylose and amylopectin content, then the starch accumulation amount was calculated. 【Result】 In the 2004-2005 growth season(in clay-soil field), 60 mm of water being irrigated respectively at pre-sowing stage, jointing stage and anthesis stage in W1 treatment (the grain yield under this treatment was 8 701.23 kg•hm-2) and 60mm of water was irrigated respectively at pre-sowing stage, pre-winter stage, jointing stage and anthesis stage in W2 treatment(the grain yield in this treatment was 9 159.30 kg•hm-2). The water consumption amount was lower in treatment W1 than that in treatment W2, while precipitation enjoy a higher percentage in water consumption amount in W1 treatment, and irrigation efficiency and water use efficiency were higher, but soil water use efficiency had no significant difference between W1 and W2 treatments. The grain weight at maturity had no significant difference among all the treatments. In the 2006-2007 growth season (in sandy-soil field), treatment W3’ under which 60mm of water was irrigated respectively at pre-sowing stage, pre-winter stage, jointing stage and anthesis stage got the highest grain yield. There was no significant difference in water consumption amount and the percentage of precipitation in water consumption amount between treatment W3’ and other irrigation treatments, while the soil water and precipitation use efficiency and water use efficiency were higher in treatment W3’ than those in other irrigation treatments, but the irrigation water use efficiency was lower in treatment W3’ than those in other irrigation treatments. The grain weight had no significant difference between treatment W3’ and W2’ which was irrigated 60 mm of water at pre-sowing stage, jointing stage and anthesis stage respectively, but were higher than that of other treatments. Treatment W1 had higher soil water relative content in 0-140 cm soil layer at pre-winter stage and anthesis stage in 0-80 cm soil layer at jointing stage than those in treatment W0 which no irrigation, then 60 mm of water was irrigated respectively at jointing stage and anthesis stage, and the treatment W1 got lower amolyse accumulation amount and higher amylopectin accumulation amount at late grain filling period and higher value of amylopectin content to amolyse content at maturity period than those in treatment W0. Treatment W2 had higher soil water relative content in 80-140 cm soil layer at jointing stage and anthesis stage than those in treatment W1, then 60 mm of water was irrigated respectively at jointing stage and anthesis stage, but the amylopectin accumulation amount, amolyse accumulation amount and grain weight had no significant change. 【Conclusion】 In clay-soil field which has better ability of water conservation, the amolyse accumulation amount was lower in W1 treatment than W0 treatment, and amylopectin accumulation amount and the ratio of amylopectin content to amolyse content in grains in W1 treatment were higher than those in W0 treatment, and the grain yield and water use efficiency were also higher. The increase of irrigation amount had no significant effect on the starch component accumulation amount and grain weight, while water use efficiency was decreased. The highest grain yield and highest water use efficiency were achieved in W3’ treatment in sandy-soil field which had lower ability of soil conservation. The result has provided a reference for the irrigation strategy of wheat production in clay-soil field and sandy-soil field.

    PLANT PROTECTION
    The Influence of Triazophos Deposition on Rice Leaves by Adding Spray Adjuvants
    SHI Ling-li,CHEN Fu-liang,ZHENG Fei-neng,WANG Yi,XIE Ming
    Scientia Agricultura Sinica. 2009, 42(12):  4228-4233 .  doi:10.3864/j.issn.0578-1752.2009.12.013
    Abstract ( 870 )   PDF (340KB) ( 1014 )   Save
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    【Object】 The influence of triazophos deposition on rice leaves were studied by adding wetting agents and thickener in the dilution of triazophos,as well as simulated rainfall after 0, 1, 2, 4, and 8 h of spraying. 【Method】 By means of pesticide residue analysis method, the deposition of triazophos on rice leaves was detected by GC. 【Result】 The results showed that the combination of the wetting agent and thickener could increase the deposition significantly and resist the erosion of rain. The best combination was 0.025% of adjuvant 6501 and 0.02% of adjuvant PAAS in dilution of triazophos. The deposition of the best combination is 77.4 μg•g-1, and it is seven times higher than that of CK treatment. 【Conclusion】 The addition of an appropriate spray adjuvant may decrease the pesticide dosage in equal field control efficacy and can achieve the goal of reducing control cost and environmental pollution.
    Quantitative Trait Loci for Adult-Plant Resistance Against Yellow Rust in a Wheat-Derived Recombinant Inbred Line Population
    YAO Qin,SONG Yan-xia,ZHOU Rong-hua,FU Ti-hua,JIA Ji-zeng
    Scientia Agricultura Sinica. 2009, 42(12):  4234-4241 .  doi:10.3864/j.issn.0578-1752.2009.12.014
    Abstract ( 1158 )   PDF (270KB) ( 747 )   Save
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    【Objective】 This study aimed at detecting quantitative trait loci (QTL) responsible for adult resistance against wheat yellow rust. 【Method】 In this study, a recombinant inbred line (RIL) population derived from a cross between Neixiang 188 and Yanzhan 1 was evaluated for adult-plant resistance (APR) in two years under field conditions using AUDPC (Area Under Disease Progress Curve, AUDPC) and IT (Infection Type, IT). 【Result】 Nine additive QTLs and seven pairs of epistatic QTLs were detected based on the composite interval mapping. Among them, five additive QTLs and two pairs of epistatic QTLs showed QTL×environment interactions (QEs). Two QTLs, QYr.caas-3D and QYr.caas-6A, were both detected using either AUDPC or IT data. The total QTL effects based on AUDPC data explained 62.05% phenotype variation, with 44.32% additive effects, 17.73% epistatic effects, and 0.42% QEs. Using IT data, the total QTLs explained 37.53%, with 23.94% additive effects, 10.51% epistatic effects, and 3.08% QEs. 【Conclusion】 The loci of adult plant resistance to yellow rust were identified not only in resistant parent Neixiang 188, but also in susceptible parent Yanzhan 1. QYr.caas-3B and QYr.caas-6D were two new loci responsible for the resistance to yellow rust disease. The interaction could occur between two loci without resistance.

    Effects of Acaricides at Sublethal Dose on Formation and Completion of Hypopi of Rhizoglyphus echinopus

    ZHANG Man-li,ZHANG Shan-xue,CAI De-jiang,FAN Qing-hai
    Scientia Agricultura Sinica. 2009, 42(12):  4242-4247 .  doi:10.3864/j.issn.0578-1752.2009.12.015
    Abstract ( 993 )   PDF (267KB) ( 740 )   Save
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    【Objective】 Hypopus of Rhizoglyphus echinopus has good resistance to the adverse environmental conditions and acaricide. In order to understand the effects of acaricides at sublethal dose on hypopus formation and completion, a series of experiments were conducted. 【Method】 The dry residual film method was applied to test the toxicity of six acaricides to protonymphs and hypopi of Rhizoglyphus echinopus. The effects of sublethal doses (LC20) of abamectin and hexythiazox on hypopus formation and completion were also studied. 【Result】 The toxity line of abamectin EC and hexythiazox EC on protonymphs were y=1.2986+0.7549x and y=1.2866+1.1189x, the toxity line of abamectin EC and hexythiazox EC on hypopus were y=1.2986+0.7549x and y= -2.3442+1.3936x .The hypopus formation was not significantly affected by 1.8% abamectin EC and 5% hexythiazox EC (P>0.05), however, only a few hypopi treated by two acaricides could reach the tritonymph stage in 49 days, 13.44% and 3.11%, respectively. 【Conclusion】 Sublethal doses (LC20) of abamectin and hexythiazox have a little influce on formation of hypopi of Rhizoglyphus echinopus, but have a great influnce on its completion.

    Influence of Red Imported Fire Ant (RIFA) on Ant Community in Different Distances Around Separate Mound
    WU Bi-qiu,LU Yong-yue,ZENG Ling,HUANG Huan-guang
    Scientia Agricultura Sinica. 2009, 42(12):  4248-4259 .  doi:10.3864/j.issn.0578-1752.2009.12.016
    Abstract ( 944 )   PDF (302KB) ( 675 )   Save
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    【Objective】 The influence of red imported fire ant (RIFA) on ant community in different distances around separate mound in mowing wasteland was studied in this paper. 【Method】 The dynamics of species and abundance of ants which in different distances around separate mound of RIFA were investigated by using pitfall and bait traps, and the principal component analysis method and diversity indexes were used to probe into the influence of RIFA on the diversity of ant community. 【Results】 The results indicated that the competition was furious between RIFA and native ants, the influence of RIFA on the diversity of ant communities at short distance was more obvious than at long distance around mound in both small and big mounds. As the infested time went on, the number of RIFA foragers became less and less. The number of all ant foragers and predominant index of ant community in 1 m of small mound increased, species richness, diversity index and evenness index of ant community decreased in June with a high proportion (45.02%) and in August with a low proportion (16.01%) of RIFA forgers. While in 1 m and 3 m of big mound, as the infested time went on, the number of RIFA foragers decreased at first and became more and more later. With a low proportion (48.36%, 1 m, June; 34.06%, 3 m, July) and a high proportion (89.94%, September, 1 m; 59.52%, September, 3 m) of RIFA forgers in big mound, the predominant index of ant community increased, diversity index and evenness index of ant community decreased. Principal component analysis also indicated that the ant species of begetting amount fluctuation and their changing rule.【Conclusion】The impact of invasion of RIFA in wasteland on the diversity of ant communities in short distance around mound was obviously. The high proportion of RIFA forgers in short distance around mounds in both small and big mounds, the species richness and the number of individuals of native ants, the predominant index of ant community increased, diversity index and evenness index of ant community decreased.

    SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT
    SOC Enhancement in Major Types of Paddy Soils in a Long-Term Agro-Ecosystem Experiment in South China. V. Relationship Between Carbon Input and Soil Carbon Sequestration
    ZHOU Ping,PAN Gen-xing,LI Lian-qing,ZHANG Xu-hui
    Scientia Agricultura Sinica. 2009, 42(12):  4260-4268 .  doi:10.3864/j.issn.0578-1752.2009.12.017
    Abstract ( 1005 )   PDF (339KB) ( 975 )   Save
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    【Objective】 The coupling of soil C sequestration with productivity is a hotspot in soil C cycling; moreover the increase of saturation capacity is a key approach in agricultural C sequestration and mitigation. The objective of this study was to clarify the saturation capacity of soil C sequestration and its cooperation with C input (crop source and organic fertilizer source C) and N input. 【Method】Data from four sites of long-term soil agro-ecosystem experiments of fertilizations from South China was collected, with which the soil cover four types of paddy soils derived from sandstone (PP) in Sichuan Basin, from Quaternary red earth (RP) in red soil region of Jiangxi, from lucustrine deposit (TP) in Tai Lake region of Jiangsu and from lagoon deposit (SP) in costal area of Zhejiang respectively). Changes in crop yields and topsoil SOC content, and the relationship of topsoil SOC with C and N input was analyzed and further the saturation capacity of soil C sequestration was explored with different fertilization treatments. 【Result】A significant linear correlation was found of annual soil C sequestration with C input under different fertilization treatments. SOC accumulation resultant from C input was high in PP and RP compared to that in TP and SP. The saturation capacity of C sequestration under good fertilization managements was shown highest in RP, followed by SP over TP and PP. Statistic analysis exhibited a negative linear correlation of C sequestration efficiency (C sequestered per unit of C input) with the initial SOC content but with the clay content. However, the saturation capacity of C sequestration was in a positive linear correlation with free iron oxyhydrates and annual precipitation for the soils studied. In addition, a positive correlation of N use efficiency on crop yields with soil C sequestration rates was observed though it was less distinctive for SP probably due to the high N stock. 【Conclusion】Well designed fertilization could promote soil C sequestration from the increased C input through increasing crop productivity. N use efficiency of rice paddies could be coupled with soil C sequestration under good fertilization practices. The abundant iron oxyhydrates not clay in the paddy soils was likely to be a key factor for soil C stock and sequestration in the paddy soils. Climate characteristics could also have exert influences on the changes in soil C stock with different paddy soils from various region of South China, which deserves further research.
    The Spatial-Temporal Changes of Grain Production and Arable Land in China
    LIU Yan-sui,WANG Jie-yong,GUO Li-ying
    Scientia Agricultura Sinica. 2009, 42(12):  4269-4274 .  doi:10.3864/j.issn.0578-1752.2009.12.018
    Abstract ( 975 )   PDF (401KB) ( 918 )   Save
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    【Objective】 The objective of this study is to reveal the spatial-temporal change characteristics of grain production and arable land changes in China as well as the sensitivity of grain yield changes to arable land from 1990 to 2005. 【Method】 Contrastive analyzing the dynamic relationship of China's grain production and arable land changes by constructing models of gravity center fitting and sensitivity analyzing in this paper. 【Result】 The results of study show that the gravity center of China’s grain production and arable land distribution are both expressed as Move to northern and middle regions, which means both of them are almost the same in space. The center of arable land had been moved 17.3 km along the track of Northwest - Southwest - Northeast, and the center of grain production had been moved 223.3 km along the track of Northeast - Southwest - Northeast. The distance between the two centres of grain production and arable land was almost contrary to grain yield. When one center is close to the other, the grain yield reduced, otherwise grain yield increased.【Conclusion】 The increase of grain yield in China is affected by unit yield and planting areas, and the sensitivity of grain yield changes to arable land is enhanced. The diminishing returns of fertilizers and pesticides show that the dependence of grain production on arable land resources is increasing. It is very important to realize a mechanism and policy innovation for guarantee of national food security and arable land protection.

    Advances in Research on Effects of Conservation Tillage on Soil Carbon
    ZHANG Hai-lin,SUN Guo-feng,CHEN Ji-kang,CHEN Fu
    Scientia Agricultura Sinica. 2009, 42(12):  4275-4281 .  doi:10.3864/j.issn.0578-1752.2009.12.019
    Abstract ( 912 )   PDF (225KB) ( 1405 )   Save
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    Soil carbon cycle plays an important role in global warming and concerns for it have been increased. Conservation tillage could reduce soil erosion, enhance residue utilization, and increase SOC, which influence soil carbon sequestration greatly. Lots of achievements in soil carbon cycle of conservation tillage have been made in the past years. With the deep studies, the understanding of mechanism of tillage effects on soil carbon has become more and more clearly and thoroughly. The effects of conservation tillage on carbon were reviewed objectively and in detail in this paper. Future trends and advices were also proposed on further researches in this field in China.
    Effect of Omethoate on Soil Enzyme Activities
    HE Wen-xiang,ZHENG Fen-li,TIAN Hai-xia
    Scientia Agricultura Sinica. 2009, 42(12):  4282-4287 .  doi:10.3864/j.issn.0578-1752.2009.12.020
    Abstract ( 1172 )   PDF (237KB) ( 852 )   Save
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    【Objective】 Omethaoate is a major pesticide that is widely used in agricultural production in the world, and it has made an important contribution to grain production increase. Its negative influence of widely use on environment pouation has been concerned by peoples as the environment is worse and the quality of agricultural products are reduced. It is necessary and important to study soil ecological toxicity of pesticides in theory and application. 【Method】 By indoor simulation, soil enzyme activities, for example, soil invertase, urease and alkaline phosphatase, which catalyze carbon, nitrogen and phosphate nutrient cycling, were assayed in soil samples polluted by different omethaoate concentrations. 【Result】 Omethaoate could remarkably inhibit soil urease activity, their relationship reached significant and remarkable significant correlation between soil urease activity and omethaoate conentration. Soil urease activity could be one of the indexes to monitor soil pollution by omethaoate. The model U=A/(1+B×C) was a better regression equation and it showed their reaction mechanism was a full inhibition between soil urease and omethaoate. The acquired critical concentrations were 0.32, 2.88 and 11.43 g a.i.•kg-1 respectively with soils polluted slightly, medium and seriously by omethaoate. The maximum change was less than 18.61% and 8.36% for soil invertase and alkaline phosphatase activity respectively. Soil invertase and alkaline phosphatase were not sensitive to omethaoate. 【Conclusion】 Soil urease is better than invertase and alkaline phosphatase to be one of indexs of soil polluted by omethaoate. The relationship between soil enzymes and pesticides is influenced by many factors such as enzyme types, pesticide variety and soil properties.

    HORTICULTURE
    Research of the Photoinhibition of PSⅠand PSⅡ in Leaves of Cucumber Under Chilling Stress Combined with Different Light Intensities
    ZHANG Zi-shan,ZHANG Li-tao,GAO Hui-yuan,JIA Yu-jiao,BU Jian-wen,MENG Qing-wei
    Scientia Agricultura Sinica. 2009, 42(12):  4288-4293 .  doi:10.3864/j.issn.0578-1752.2009.12.021
    Abstract ( 997 )   PDF (353KB) ( 1191 )   Save
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    【Objective】 The purpose of this study is to explore the effect of different light intensities under chilling temperature on photosystemⅠ(PSⅠ) and photosystemⅡ(PSⅡ) in cucumber leaves and the interaction between PSⅠand PSⅡ. 【Method】 By simultaneously analyzing chlorophyll a fluorescence transient and light absorbance at 820 nm as well as analyzing chlorophyll quenching, the effects of chilling stress (4℃) combined with different light intensities (0, 100, 200, 400 μmol•m-2•s-1) on photoinhibition of PSⅠand PSⅡin cucumber leaves were studied. 【Result】 The results showed that the maximal photochemical efficiency of PSⅡ (Fv/Fm) continually decreased with the increase of excess excitation energy ((1-qP)/NPQ). The maximum PSⅠredox activity (△I/Io) also decreased significantly with the increase of excitation energy when excitation energy was relatively lower. However, when the excess excitation energy exceeded a certain level, the △I/Io did not increase obviously with the increase of the excess excitation energy any more. 【Conclusion】Excess excitation energy under chilling temperature resulted in photoinhibition not only in PSⅠbut also in PSⅡ. However, too much excess excitation energy resulted in severely decrease in PSⅡactivity (Fv/Fm), which limited the transfer of photosynthetic electron from PSⅡ to the PSⅠprotecting the PSⅠfrom further inhibition.

    Study on High-Resolution 5S rDNA –FISH of Brassica oleracea Chromosome 2
    WANG Yong,ZHU Li-quan,RONG Xiao-ying,CHEN Xiao-dan,TANG Zhang-lin,WANG Xiao-jia
    Scientia Agricultura Sinica. 2009, 42(12):  4294-4300 .  doi:10.3864/j.issn.0578-1752.2009.12.022
    Abstract ( 964 )   PDF (423KB) ( 845 )   Save
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    【Objective】 The aim of this experiment is to localize 5S rDNA on the chromosomes of Brassica oleracea var. acephala by FISH (fluorescence in situ hybridization) and estimate the copy number of 5S rDNA, further provide a basis for the location of genes and the construction of cytogenetic map of chromosome 2 by FISH. 【Method】 B. oleracea var. acephala was used as the experimental material, 5S rDNA probe was labeled by PCR-DIG(PCR-digoxigenin). By FISH, 5S rDNA probe was located on the metaphase chromosomes of tapetal cell, pachytene chromosomes and extended DNA fibers (EDF). 【Result】 In the metaphase chromosomes of tapetal cell and pachytene chromosomes, it was observed that three closely adjacent 5S rDNA hybridization signal sites (a, b, c) located near the centromere in the long arm of the submetacentric chromosome 2, the intensity of signals is b>a>c. In the extended DNA fibers, it was estimated that the physical size of three different stretches of beads-on-string (a, b, c) is of the order of 257 kb, 359 kb, and 134 kb, respectively. 【Conclusion】 It was proved that B.oleracea var. acephala contains three tandem repeat sites, and estimated that the copies number of three 5S rDNA locus are about 510, 712, and 266, respectively.

    Screening and Evaluation of Tomato Rootstocks for Resistance to Meloidogyne incognita
    JIA Shuang-shuang,GAO Rong-guang,XU Kun
    Scientia Agricultura Sinica. 2009, 42(12):  4301-4307 .  doi:10.3864/j.issn.0578-1752.2009.12.023
    Abstract ( 957 )   PDF (292KB) ( 1275 )   Save
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    【Objective】 In order to screen the resistant tomato rootstocks, the screening method and the resistance to Meloidogyne incognita were studied in the experiment. 【Method】 The relative growth rate and relevant disease resistant index of 16 tomato rootstock seedlings planted in pots and inoculated with M. incognita were measured after 50 days, moreover cluster analysis and subordinate function were used in the experiment. 【Result】 The results showed that different relative growth rates of different organs, different disease resistant indexes of 16 tomato rootstock seedlings and their different coefficient variances resulted from the infection of M. incognita were different, in which the coefficient variances of relative root fresh mass of five relative growth rate was 20.20%, the highest, but that of relative stem diameter was only 6.22%. The results of clustering analysis with the relative growth rate and the disease resistant index were same as GI, but be a little different from the results of clustering analysis with DI. By the method of subordinate function, the sequence of resistance from strong to weak successively was determined.【Conclusion】 The result of cluster analysis with multiple indexes and total indexes was more accurate than other single index for the resistant class of tomato rootstocks to M. incognita. By the method of clustering analysis and subordinate function, Baliya is determined as an immune material; BESUPA, Banzhen No.2, Rootstock 606, Rootstock002, Rootstock001, TMS-150, SUPPORT, MIKADO, Rootstock 401 are resistant materials; Anka-T is a disease tolerant material; Banzhen No.1 and Beijing No.1 are sensitive materials; 128, BF xingjing 101, and LS-89 are highly sensitive materials.

    Effects of Cinnamon Acid on Respiratory Rate and Its Related Enzymes Activity in Roots of Seedlings of Malus hupehensis Rehd.
    GAO Xiang-bin,ZHAO Feng-xia,SHEN Xiang,HU Yan-li,HAO Yun-hong,YANG Shu-quan,SU Li-tao,MAO Zhi-quan
    Scientia Agricultura Sinica. 2009, 42(12):  4308-4314 .  doi:10.3864/j.issn.0578-1752.2009.12.024
    Abstract ( 925 )   PDF (342KB) ( 897 )   Save
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    【Objective】 The objectives of this research were to study the effect of different concentrations of cinnamon acid application on respiratory rate and its key enzymes activity in roots of seedlings of Malus hupehensis Rehd. The mechanisms were discussed so as to provide a basis for further study on the cause of diseases in apple continuous cultivation. 【Method】 Twenty milliliters of different concentrations of cinnamon acid were added to the containers, and the respiratory rate of roots was measured by solution OXY-LAB oxygen electrodes under 25℃ and the key enzymes’ activity was also determined. 【Result】 At 5 and 25 mg•kg-1(soil), the total respiratory rate had a similar trend with tricarboxylic acid cycle (TCA) and pentose phosphate pathway(PPP) , T1, and T2 were higher than the control on the 1st day and then declined three days late but they had a recover on the last day. Embden-Meyer-hot-Parnas pathway (EMP) had a declined trend forever. The respiratory rate of the total and three pathways were decreased under 125 mg•kg-1(soil). The enzyme activity of pyrophosphate-dependent phosphofructokinase (PFK), glucose-6-phosphate dehydrogenase (G-6-PDH) and malate dehydrogenase (MDH) were similar to relative respiratory pathway. 【Conclusion】 Cinnamon acid destroyed the respiratory mechanism of roots of seedlings of Malus hupehensis Rehd., and reduced the respiratory rate of total, EMP, TCA and PPP, meanwhile, reduction of respiratory rate are increased with the improvement of concentration of cinnamon acid. Three enzymes have a similar trend with respiratory rate.

    STORAGE·FRESH-KEEPING·PROCESSING
    Effects of Ozone Treatment on Storage Quality and Physiology of Huanghua Pear Fruit
    ZHU Ke-hua,YANG Zhen-feng,LU Sheng-min,CHEN Wei
    Scientia Agricultura Sinica. 2009, 42(12):  4315-4323 .  doi:10.3864/j.issn.0578-1752.2009.12.025
    Abstract ( 925 )   PDF (409KB) ( 756 )   Save
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    【Objective】 Effects of ozonated water treatments on storage quality and physiology of whole and fresh cut Huanghua pear fruits were investigated. 【Method】 2.5 mg•L-1, 5 mg•L-1ozonated water and distilled water were used to treat the whole and fresh cut pear fruits respectively and the quality and physiology changes were monitored periodically during storage. 【Result】 The results showed that the respiration and ethylene production patterns of Huanghua pear fruit, both in the whole and fresh cut, showed peak shape change patterns. Ozone treatments significantly inhibited respiration rate and ethylene evolution of the whole fruit at 20℃ and fresh cut fruit at 5℃ respectively, but had no effects on the appearance time of climax. In addition, the ozone treatments also significantly inhibited the increase of malondiadehyde (MDA) content and relative conductance, maintained cell membrane integrity, significantly decreased browning index, delayed the decrease of total soluble solids (TSS), titratable acidity (TA) and vitamin C (Vc) in fruit tissue, thus maintained higher storage quality. 【Conclusion】 The ozone treatments can maintain higher storage quality of the whole and fresh cut Huanghua pear fruits, and the effect of 2.5 mg•L-1 is better than 5 mg•L-1 ozonated water treatment.

    Advances in Research of Aroma Compounds of Citrus Processing Products
    FAN Gang,QIAO Yu,YAO Xiao-lin,XU Xiao-yun,WANG Ke-xing,PAN Si-yi,DUAN Ya-qing
    Scientia Agricultura Sinica. 2009, 42(12):  4324-4332 .  doi:10.3864/j.issn.0578-1752.2009.12.026
    Abstract ( 956 )   PDF (310KB) ( 1145 )   Save
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    Aroma component is one of the main factors affecting the characteristic flavor and total quality of citrus processing products. Studies on aroma compounds in citrus processing products are important in preserving the characteristic flavor, preventing the loss of aroma compounds during processing and storage, enhancing the total aroma quality. In this paper, the aroma compounds in citrus processing products were introduced. In addition, the extraction of aroma compounds in citrus products, the identification of aroma-active compounds, the determination of odor thresholds of aroma compounds in citrus fruits and the changes of aroma compounds during processing and storage were summarized. Moreover, the problems and development trend in the future of aroma compounds in citrus fruits were discussed.

    Studies on the Characteristics of Linoleate Isomerase from P. freudenreichii ssp. shermanii and L. plantarum
    WANG Li-min,LÜ,Jia-ping,DUAN Yu-quan,LIU Lu
    Scientia Agricultura Sinica. 2009, 42(12):  4333-4340 .  doi:10.3864/j.issn.0578-1752.2009.12.027
    Abstract ( 1039 )   PDF (613KB) ( 918 )   Save
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    【Objective】 The attributes of linoleate isomerases from both Propionibacteriu freudenreichii ssp. shermanii and Lactobacillu plantarum were studied to understand the different characters of linoleate isomerase originated from different strains. 【Method】 Dynamic and characteristic parameter such as Michaelis constant, molecular weight of linoleate isomerase with different origins were determined using the method of enzyme activity analysis and electrophoresis. The peptide mass fingerprints produced by P. freudenreichii ssp. shermanii and L. plantarum linoleate isomerase were studied by HPLC–ESI–MS system. 【Result】 The optimum pH for P. freudenreichii ssp. shermanii linoleate isomerase was 8.0 and the optimum temperature was about 30℃. The Km and Vmax of linoleic acid was 20.53 μmol•L-1 and 0.44 μg•ml-1•min-1. The optimum pH for L. plantarum linoleate isomerase was 7.5 and the optimum temperature was about 50℃. The Km and Vmax of linoleic acid was 17.85 μmol•L-1 and 0.73 μ•ml-1•min-1, respectively. Molecular structure of linoleate isomerase produced by P. freudenreichii ssp. shermanii and L. plantarum showed some extent homogeny on sequences. 【Conclusion】 The linoleate isomerase originated from the two strains has different attributes. The sequences of both linoleate isomerases have some extent homogeny and the characteristic parameters of experimental linoleate isomerase are different from those of reported.

    ANIMAL SCIENCE·RESOURCE INSECT
    Developmental Expression and Tissue Distribution of Porcine Cathepsin B and Cystatin B mRNA
    CHEN Lei,WANG Jin-yong,LI Xue-wei,LIU Liang
    Scientia Agricultura Sinica. 2009, 42(12):  4341-4348 .  doi:10.3864/j.issn.0578-1752.2009.12.028
    Abstract ( 1159 )   PDF (492KB) ( 902 )   Save
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    【Objective】 The tissue- and developmental-specific expression of cathepsin B (CTSB) and cystatin B (CSTB) mRNA transcripts in porcine were studied in order to understand the genetic mechanism of tenderness traits. 【Method】 The expression of CTSB and CSTB mRNA in different tissues and different development phases were revealed by Taqman real-time PCR analyses. 【Result】 In adult pigs, CTSB mRNA and CSTB mRNA expression were widely detected in many tissues. The highest level of expression occurred in kidney and the lowest were in myocardium and skeletal muscle, respectively. The quadriceps femoris muscle had significantly higher CSTB mRNA expression than m. longissimus dorsi muscle (P<0.01). The expression of CTSB mRNA in m. longissimus dorsi muscle increased first then decreased in 0-5-month-old of age of Landrace and Meishan pigs. The peak of expression occured earlier in Meishan than in Landrace. Continuously decreased expression patterns of CSTB mRNA were detected among different months in m. longissimus dorsi muscle of Meishan pig. And in Landrace pig the expression peaked at 2 months of age, then decreased. 【Conclusion】 These results indicate that the expression of CTSB and CSTB mRNA are influenced by tissue, developmental phases and breed factors. There is a significant positive correlation between CTSB and CSTB mRNA expression.

    Nonphytate Phosphorus Requirement and Efficacy of a Genetically Engineered Yeast Phytase on Yellow Broilers at 22-42-Day-Old of Age
    JIANG Zong-yong,LIN Ying-cai,ZHOU Gui-lian,LI De-zhong,JIANG Shou-qun,XI Peng-bin,ZHANG Han-xing
    Scientia Agricultura Sinica. 2009, 42(12):  4349-4357 .  doi:10.3864/j.issn.0578-1752.2009.12.029
    Abstract ( 867 )   PDF (253KB) ( 709 )   Save
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    【Objective】 An experiment was conducted to investigate the requirement of nonphytate phosphorus (NPP) and efficacy of a genetically engineered yeast phytase (PHY A) by Lingnan yellow broilers from 22 d to 42 d. 【Method】 A total of 1320 one-day-old male chicks were randomly divided into eleven dietary treatment groups, which consisted of four replicate floor pens with 30 birds per pen. The control group (Treatment 1) was fed with basal diet of NPP 0.08% without dicalcium phosphate or phytase supplementation. Dietary levels of NPP were 0.16, 0.24, 0.32, 0.40, 0.48, and 0.56%, respectively, for treatments 2 to 7, through addition of dicalcium phosphate (chemistry grade) to the basal diet. Diets of treatments 8 to 11 were supplemented with PHY A at 200, 400 and 600 U•kg-1, a commercial phytase product (PHY B) at 400 U•kg-1 level, respectively. 【Result】 The birds in 0.32%-0.56% NPP groups gained more than those of the other groups (P<0.05). The NPP supplementation significantly improved feed intake (P<0.05). The feed gain ratio was significantly decreased by 0.40% NPP diet compared to the control birds (P<0.05). The level of 0.48% NPP was required for optimum tibia development. The additions of PHY A at 400 U•kg-1 and 600 U•kg-1 level and PHY B all significantly improved ADG (P<0.05), ADFI (P<0.05), and dry defatted tibia weight (P<0.05). And the percentage of tibia ash was increased by 600 U•kg-1 PHY A supplementation (P<0.05).【Conclusion】The requirements of NPP for maximal ADG and highest percentage tibia ash both are 0.40%. The phosphorus equivalency value of PHY A is estimated at 685 U•kg-1 for male yellow broilers of 22-42 day old.

    VETERINARY SCIENCE
    Effects of Enrofloxacin on Human Intestinal Mocrofloa in Chemostat Model
    QIN Yu-ling,ZHAO Jing-jing,HUANG Xian-hui,GAO Yan-ling,CHEN Zhang-liu,ZENG Zhen-ling,LIU Jian-hua
    Scientia Agricultura Sinica. 2009, 42(12):  4358-4365 .  doi:10.3864/j.issn.0578-1752.2009.12.030
    Abstract ( 958 )   PDF (326KB) ( 715 )   Save
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    【Objective】 The effects of low concentrations of enrofloxacin on human intestinal microflora were evaluated by using chemostat models of the human large bowel, which would be helpful for establishing the microbiological ADI of enrofloxacin. 【Method】 Four steady state microbial communities were established in chemostats using inocula from human feces. Three chemostats were exposed to enrofloxacin (0.2, 2, and 20 mg•L-1, respectively) for 8 days; one served as a no-drug control. Changes of bacterial populations, the relative levels of resistant aerobic and facultative anaerobes to ciprofloxacin, colonization resistance against exogenous Salmonella, and the microflora structure after drug exposure were assayed by plate counting method and PCR-DGGE. 【Result】 Results showed that 2 mg•L-1 and 20 mg•L-1 enrofloxacin significantly reduced flora populations of total aerobic and facultative anaerobes, Escherichia.coli, Enterococcus, and the total anaerobic bacteria (P<0.05 or P<0.01). The percentages of resistant aerobic and facultative anaerobes and E.coli were increased significantly after treatment with 0.2, 2 and 20 mg•L-1 enrofloxacin (P<0.05 or P<0.01). Seven days after treatment, the percentages of resistant aerobic and facultative anaerobes, and E.coli were decreased, but still higher than the control group (P<0.01). The number, Shannon’s diversity index and Sorenson’s similarity index of PCR-DGGE lanes decreased significantly after treatment with 20 mg•L-1 enrofloxacin (P<0.05 or P<0.01). The colonization barrier against Salmonella was disrupted by 2 mg•L-1 and 20 mg•L-1 enrofloxacin. 【Conclusion】 0.2 mg•L-1 enrofloxacin (equal to 3.67 μg•kg-1 b.w.) can increase the percentage of resistant aerobic flora to ciproflxacin. Thus the ADI (6.2 μg•kg-1 b.w.) of enrofloxacin and ciprofloxacin established by Ministry of Agriculture may affect human intestinal microflora by selecting resistant aerobic flora.

    Differentiation of Wild-Type Viruses and HCLV Vaccine of Classical Swine Fever Virus by One-Step Fluorescent Quantitative PCR Using TaqMan-MGB Probe Technology
    LIU Jun,WANG Qin,FAN Xue-zheng,XU Lu,ZHAO Qi-zu,HUANG Wei,TANG Bo,SHA Sha,ZHOU Yuan-cheng,CHEN Lei,ZOU Xing-qi
    Scientia Agricultura Sinica. 2009, 42(12):  4366-4371 .  doi:10.3864/j.issn.0578-1752.2009.12.031
    Abstract ( 1016 )   PDF (455KB) ( 1022 )   Save
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    【Objective】 A rapid one step fluorescent quantitative PCR assay using TaqMan minor-groove-binding (MGB) probe was developed and evaluated to discriminate wild-type stains from wild-type stains and hog cholera lapinized virus (HCLV) strain of classical swine fever virus (CSFV). 【Method】 A pair of universal primer and a wild-type specific MGB probe were designed from the conservative region of CSFV genome. Then the method was optimized. Specificity, sensitivity, and conformity were tested. 【Results】 The detection results of 24 quality-control samples showed good specificity. The sensitivity of the assay was 5.3×10-2pg viral RNA per reaction, and showed a good linear relationship at a range of 100-10-7. The coincidence rate was 94.3% in detecting 122 clinical samples compared with RT-nPCR (115/122),and the positive coincidence rate and negative coincidence rate were 86.4% (38/44) and 98.7% (77/78), respectively, Kappa value was 0.87>0.75. 【Conclusion】 The one-step TaqMan-MGB PCR is able to differentiate wild-type and HCLV of CSFV strain, which shows good specificity, sensitivity and also shows high consistency of TaqMan-MGB Probe Technology and RT-nPCR method. This assay may serve as a useful diagnostic tool for CSF.

    AGRICULTURAL ECONOMY & MANAGMENT
    Estimation of Optimal Size of Financial Subsidies to Banana Windstorm Insurance——Evidence from 681 Banana Planters in Hainan Province
    PAN Yong-hui
    Scientia Agricultura Sinica. 2009, 42(12):  4372-4382 .  doi:10.3864/j.issn.0578-1752.2009.12.032
    Abstract ( 1153 )   PDF (378KB) ( 1260 )   Save
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    【Objective】 The applicability of technique applied in banana windstorm insurance of China to induce the greatest willingness to pay of contingent valuation was explored, and the decision basis was provided for policy makers to adjust and perfect the financial subsidy system of banana windstorm insurance. 【Method】 Based on the field survey of 681 banana planters in Hainan province, the optimum questionnaire format of CVM, dichotomous choice format was adopted, using the single-bounded and double-bounded dichotomous contingent valuation technique to estimate the optimal size of financial subsidies to banana windstorm insurance accurately. 【Result】 The results show that the mean greatest willingness to pay of premium rate single-bounded and double-bounded estimation were 19.25% and 17.54%, respectively. The 95% confidence interval of greatest willingness to pay estimated by these two questionnaire formats were 18.77%-19.79% and 16.89%-17.72%, respectively. 【Conclusion】 Compared with the single-bounded questionnaire format, the double-bounded questionnaire format could obtain more information about the respondents′ willingness to pay, hence, it reduces the confidence interval range and improves the estimation accuracy of the greatest willingness to pay. At the pilot practice for three continuous years, the percent of banana windstorm insurance pilot size in the total banana planting area was less than 10% in Hainan. Combined with this research result, the author holds that Hainan finance should strength the subsidy to banana windstorm insurance on the basis of 30% subsidy in two continuous years of 2007 and 2008, and the subsidy percentage should try to achieve 40%. At the same time, the premium rate should not be too low, 18% is suitable. According to this, the author puts forward two recommendations of promoting the sustainable development of agricultural insurance: To build a long-term mechanism of financial support for policy-oriented agricultural insurance and build a moderate mandatory policy-oriented agricultural insurance system.

    RESEARCH NOTES
    Effects of Different Exogenous Hormones on Physiological Characteristics of Main Stem Leaves at Flower and Boll Stage in Cotton
    ZHENG Sha-sha,SUN Chuan-fan,SUN Hong-chun,LIU Lian-tao,ZHAO Jin-feng,LI Cun-dong
    Scientia Agricultura Sinica. 2009, 42(12):  4383-4389 .  doi:10.3864/j.issn.0578-1752.2009.12.033
    Abstract ( 924 )   PDF (232KB) ( 861 )   Save
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    【Objective】 The effects of spraying exogenous hormones(gibberellin-3, GA3; brassinolide, BR and 6-benzylaminopurine, 6-BA) at early (July 6) and full (July 17) flowering time on physiological characteristics of main stem functional leaves at flower and boll stage. 【Method】 Three exogenous hormone treatments were emploid, with two concentration levels each, i.e. GA3 10 and 30 mg•L-1, BR 0.01 and 0.1 mg•L-1, and 6-BA 10 and 100 mg•L-1,with spraying water as the control(CK). The contents of chlorophyll, soluble protein, malondialdehyde, soluble sugar, endogenous hormone contents and the activities of superoxide dismutase (SOD) and peroxidase (POD) were measured. 【Result】 The results showed that the treatment of 100 mg•L-1 6-BA significantly increased the contents of chlorophyll, soluble protein, soluble sugar and the activities of SOD and POD at full flowering stage. The treatments of 6-BA 100 mg•L-1 and GA3 30 mg•L-1 significantly increased the contents of GA3 and ZR, and the ratio of ZR (zeatin riboside) / ABA (abscisic acid ),GA3 /ABA, and (ZR+GA3)/ABA at the same stage. The content of MDA greatly decreased. The treatments of BR (0.01 mg•L-1) and GA3 (10 mg•L-1) also had a positive effect, but the effect was weaker. The treatments of GA3 30 mg•L-1 and 6-BA 100 mg•L-1 significantly increased the content of soluble protein, the ratio of GA3/ABA, GA3+ZR/ABA, while the content of MDA significantly decreased at full bolling stage.【Conclusion】 Under this experimental condition, spraying 6-BA 100 mg•L-1 and GA3 30 mg•L-1 had significantly effects on the physiological functions of main stem functional leaves, it could be used for delaying the senescence of cotton.

    Effect of Low Temperature on Sucrose Transportation from Leaves to Apical Meristem in Tobacco
    SHI Yong-chun,LIU Wei-qun,WANG Xiao-yan
    Scientia Agricultura Sinica. 2009, 42(12):  4390-4395 .  doi:10.3864/j.issn.0578-1752.2009.12.034
    Abstract ( 892 )   PDF (442KB) ( 928 )   Save
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    【Objective】 To investigate the potential effects of sucrose on floral induction of tobacco, the contents and transportations of sucrose in different tissues were studied in tobacco treated with low temperature. 【Method】 After treatment with 4℃ for 24 h, the sucrose and H2O2 contents, the gene expression levels and activities of SPS and SUT1, and the redox states of ascorbate pool in different tissues of tobacco were analyzed in treated and control plants. 【Result】 In low temperature treated plants, sucrose contents increased gradually in leaves, petioles, stems and apical meristem. The activities of SPS increased and that of amylase decreased, respectively, and starch contents increased in leaves. The up-regulated expressions of SPSC in leaves and SUT1 in lateral vein were found. Moreover, the increased H2O2 contents, the tendency for ascorbate to oxidize in lateral vein, petiole, stem and apical meristem were also observed. 【Conclusion】 The results suggested that the sucrose transportation from leaves to apical meristem was enhanced by low temperature, resulting from the stimulated loading and unloading of SUT1 in phloem by the oxidation tendency in cytosolic circumstance.

    Effects of Water Management Patterns in Spring Maize Cultivation on the Dryland Soil Temperature Dynamics in the Loess Plateau
    ZHU Lin,LIU Yi,XU Hong-min,CHEN Xin-ping,LI Shi-qing
    Scientia Agricultura Sinica. 2009, 42(12):  4396-4402 .  doi:10.3864/j.issn.0578-1752.2009.12.035
    Abstract ( 918 )   PDF (331KB) ( 824 )   Save
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    【Objective】 According to the investigations on soil temperature dynamics in the spring maize growing season, a field experiment was conducted to examine the effects of different maize culturing practices on soil temperature dynamics and soil accumulated temperature (>10℃) in Loess Plateau. Hence it has a certain reference value for understanding the influence of different culturing practices on spring maize growth. 【Method】 Shendan10 was used as the test crop. Three field water management practices, including supplementary irrigation (SI), rain-fed (RF) and plastic film mulching (FM), were adopted in spring maize fields in 2007. Maize straw mulching (SM) was added in 2008. The experiment was arranged in a randomized complete block design. 【Result】 The results showed that the plastic film had a significant warming effect while the maize straw mulching had a cooling effect. During the course of crop growing season, soil accumulated temperature (>10℃) had the peak at vegetative growth stages (VE-R1), followed by growth stages (R1-R6), and reached the lowest value at seedling stage (PT-VE). Moreover, soil accumulated temperature was different in different culturing practices during the course of crop growing stages. Soil accumulated temperature was lower in the PT-VE period in plastic film mulching treatment, VE-R1 period in supplementary irrigation treatment and R1-R6 period in rain-fed treatment.The response of plastic film mulching soil temperature was most sensitive to air temperature, and the response of straw mulching soil temperature was most insensitive to the air temperature. The crop grain yield was significantly improved under both the supplementary irrigation and the film mulching treatments compared to the rain-fed treatment, with the increases of 30.0% and 18.1% in 2007, and 25.7% and 29.7% in 2008, respectively. 【Conclusion】 It is concluded that different maize culturing practices in the Loess Plateau not only affect the soil temperature dynamic changes, but also affect the maize growth and the utilization of the soil heat resources.

    Detection of Tilletia controversa Kühn by Real Time Quantitative PCR
    NIAN Si-ji,YUAN Qing,YIN You-ping,CAI Jun,WANG Zhong-kang
    Scientia Agricultura Sinica. 2009, 42(12):  4403-4410 .  doi:10.3864/j.issn.0578-1752.2009.12.036
    Abstract ( 1166 )   PDF (1000KB) ( 862 )   Save
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    【Objective】 To detection of Tilletia controversa Kühn (TCK) sensitively and accurately, real-time PCR systems were developed. 【Method】 The species-specific primer pair CQUTCK4/CQUTCK5 and probe CQUP1 were designed based on a selected specific fragment (1 322 bp) specific for TCK, and the SYBR Green I and TaqMan quantitative PCR detection systems were established with optimized reaction conditions. 【Result】 The detection limit of the two systems were 0.1fg, equal to 2.31×104 copies, which was 102-103 fold higher than conventional PCR. By the constructed detection systems, the TCK and Tilletia caries (DC)Tul (TCT) could be distinguished. The teliospore and mycelium of TCK in the infected wheat plant tissue also could be identified accurately and rapidly. 【Conclusion】 The earlier diagnosis approaches of wheat durwf bunt pathogen were set up using the two real-time PCR systems.

    Cloning and Sequence Analysis of lef-3 Gene from Pieris rapae Granulovirus
    MENG Xiang-kun,GONG Cheng-liang,XUE Ren-yu,CAO Guang-li,ZHU Yue-xiong
    Scientia Agricultura Sinica. 2009, 42(12):  4411-4419 .  doi:10.3864/j.issn.0578-1752.2009.12.037
    Abstract ( 1174 )   PDF (432KB) ( 734 )   Save
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    【Objective】 To better understand the origination and evolution of lef-3 gene from baculovirus genome and determine the relationships between various viruses at molecular level. 【Method】 Late expression factor 3 gene (lef-3) fragments of Pieris rapae granulovirus was obtained through conventional PCR method, sequenced after being cloned into T-vector. Then, bioinformatics analysis on lef-3 gene and its encoding sequences were conducted by using bio-softs. 【Result】 The DNA fragment was cloned and 4 mutations were appeared ,which did not alter the sequences of amino acids. It was inferred that PiraGV LEF-3 protein contained 399 amino acids ,the total molecular of which was 3.99 kD. Prediction of the lef-3 advanced structure and homology comparison between its encoding sequences and other LEF-3 genes from various baculoviruses showed that the lef-3 gene might encode the single strand DNA-binding protein .The result of blast revealed that the lef-3 gene only existed in Lepidoptera host for the baculovirus genome,and the evolution analysis illustrated that lef-3 gene could be divided into three groups including one GV genus and two NPV genus. The selection pressure analysis of GV's lef-3 gene coding region showed that the majority of lef-3 genes performed negative selection, while the Ka/Ks differed from different lef-3 genes, to some extent ,which also performed positive selection. The origination analysis revealed that lef-3 gene of baculovirus might derive from bacteria. 【Conclusion】 The lef-3 gene of PiraGV was cloned successfully and speculations upon the possible pattern of origination and evolution were made through bioinformatics analysis.

    Risk Analysis of Spiralling Whitefly (Aleurodicus Disperses Russell) in Mainland of China
    TANG Chao,YU Peng,PENG Zheng-qiang,FU Yue-guan,WAN Fang-hao
    Scientia Agricultura Sinica. 2009, 42(12):  4420-4427 .  doi:10.3864/j.issn.0578-1752.2009.12.038
    Abstract ( 920 )   PDF (353KB) ( 925 )   Save
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    【Objective】 Based on the results of risk analysis, the strategies for risk prevention of Aleurodicus dispersus Russell were proposed, which are expect to reduce the risk to a minimal level. 【Method】 Based on the rule of pest risk analysis of international standards for pest measurement, the paper dealt with the quantitative and qualitative analysis of the risk level of A. dispersus in four aspects – the distribution, possibilities of invasion and spreading, potential damaged degree and economic importance. 【Result】 The comprehensive risk value of A. dispersus Russell was 2.52, which indicated that A. dispersus is a high risk pest.【Conclusion】A. dispersus will be a terribly high risk pest in mainland of China, it should be added to quarantine pest list and be put under quarantine.

    Cloning and Sequence Analyses of Genes Coding for CD3ε,CD4, CD8α of Sheldrake and Cherry Valley Duck
    LI Wei,BAI Jia-yuan,XU Yuan-yuan,GU Chang-qin,ZHANG Wan-po,CHENG Guo-fu,CHEN Min,HU Xue-ying
    Scientia Agricultura Sinica. 2009, 42(12):  4428-4434 .  doi:10.3864/j.issn.0578-1752.2009.12.039
    Abstract ( 871 )   PDF (521KB) ( 747 )   Save
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    【Objective】 Comparative analyses were conducted employing the coding sequences of the CD3ε, CD4, and CD8α genes from ducks of certain Chinese domestic breeds, which provide a fundamental basis for the investigation of their structure and function, and potential application of related antibodies. 【Method】 The complete coding sequences of CD3ε, CD4, and CD8α of Sheldrake and Cherry Valley ducks were isolated by RT-PCR assay, and sequence alignment was made employing ClustalX(1.83) and DNAstar software by aligned with the corresponding coding sequences of Pekin duck (published in the GenBank, and accession no: AF378704, AF378701 and AF378373). 【Result】 The identities of pair-wise nucleotide and amino acid sequences of CD3ε and CD4 genes have reached to 99% among Pekin duck, Sheldrake and Cherry Valley duck. As for CD8α, the nucleotide sequences similarities between Pekin duck and Sheldrake reached 99%, whereas it was 95% for Pekin duck and Cherry Valley duck. Furthermore, the amino acid sequence of CD8α is the same for both Sheldrake and Cherry Valley duck, whereas the protein sequence of CD8α of Cherry Valley duck contained 16 different amino acids, including 15 amino acids in the extracellular area, which result in the obvious differences in protein hydrophilicity, antigenicity and the possibilities to be exposed on protein surface.The phylogenetic and evolutionary relationships of the nucleic acid sequences of these genes were analysed, the result showed a close relationship among Cherry valley duck ,Sheldrake and Pekin duck in genes coding for CD3ε and CD4, but Cherry valley duck had a remote relationship with Sheldrake and Pekin duck in genes coding for CD8α. 【Conclusion】 There is a low diversity among Cherry valley duck ,Sheldrake and Pekin duck in genes coding for CD3ε and CD4, but Cherry valley duck has a considerable diversity with Sheldrake and Pekin duck in genes coding for CD8α.

    Genetic Variation of Growth Hormone Gene on Ningqiang Pony
    ZHANG Tao,LU Hong-zhao,CHEN Rui,ZHOU Jian-jun
    Scientia Agricultura Sinica. 2009, 42(12):  4435-4440 .  doi:10.3864/j.issn.0578-1752.2009.12.040
    Abstract ( 989 )   PDF (260KB) ( 911 )   Save
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    【Objective】 In order to provide a scientific basis for the origin and dwarfish mechanism of Ningqiang pony, the sequence of GH of different animals and some China horse breeds were studied. 【Method】 The PCR amplification, sequencing and DNAMAN software were used. 【Result】 There were 29 genetic variation, 11 loci were found in the coding region. Taking the AA sequence of GH of Mongolia horse as standard, there was 1 amino acid variation in Ningqiang pony. Through clustering analysis based on the sequence of mRNA of GH, the homology was 100%, 99.8%, 99.7%, between Ningqiang and Debao 67, Baise and Mongolia. 【Conclusion】 The homology level of GH was higher among Equus caballus, Sus scrofa and dog in present study, and the mutation of G→A affects the growth of Ningqiang pony.