Abstract: The effect of media components on the induction of shoot regeneration for different types of explants in three genotypes of sunflower was investigated. The result showed that it was easy to form the callus for different genotypes explants cultured on media containing suitable 3-indoleacetic acid (IAA)) and 6-benzylaminopurine (6-BA) concentrations, but it was difficult of shoot regeneration from callus. The frequency of shoot regeneration from different types of explants for all the genotypes sunflower are in turn as followed: cotyledonary nodes > hypocotyls> cotyledons> leaves. Best shoot regeneration rates were obtained in the presence of 1.2mg•L-1 6-BA for cotyledonary nodes, 1.8mg•L-1 BA for hypocotyls and 0.6g•L-1 6-BA for cotyledonary. The shoot didn’t regenerate from leaves. The ability of inducing shoot regeneration for different genotype sunflower was very different and the regenerated frequency of PR29 was highest.

Key words: Sunflower, Explant, Tissue culture, Plant regeneration