蔷薇属植物类根体和类原球茎发生发育的影响因子

doi:10.3864/j.issn.0578-1752.2011.22.011

Abstract

Abstract: 【Objective】 Leaf explants of multiple species of Rosa spp. were used for investigation of the factors influencing the occurrence and development of rhizoid and protocorm-like body in this paper，which can lay a foundation for revealing the occurrence mechanism of PLB and molecular breeding. 【Method】 The growth of rhizoid and protocorm-like body were observed and statistics were made by changing the medium components, different growth regulators and their concentrations, culture conditions and the integrity of the rhizoid on the Murashige and Skoog (MS) medium.【Result】The results showed that the occurrence and size of the rhizoid was different depending on the type of auxin, but not cytokinin. Rhizoid could be induced from leaf explants of Rosa spp. on the Murashige and Skoog (MS) medium containing any type of the three auxins. On the other hand, rhizoid could be induced from the leaf explants only in dark, however, this process was significantly suppressed under light condition. On the contrary, lighting promoted the matured rhizoid turning green and developed into protocorm-like body(PLB), but this process did not happen under dark condition and light-dark condition without TDZ. Cutting part of the matured rhizoid from the top, the rhizoid could also develop into PLBs, whereas, the rhizoid cut from the callus surface, couldn’t grow into PLBs, but rather turn brown and died, whether they were placed horizontally or vertically. This indicated that the tip of the rhizoid was not essential for the PLBs formation, but the bottom of rhizoid was vitally important for TDZ absorption, loss of the callus on the rhizoid bottom affected the absorption and transportation of TDZ, and further, the development of the protocorm-like body was suppressed. 【Conclusion】 This method is of universality for the rhizoid induced by auxin. Lighting is a inhibitor for the occurrence of the rhizoid but a promoting factor for the information of the PLB, lighting and TDZ has a synergistic action on the process of rhizoid developing into protocorm-like body. The tip of rhizoid is not the essential for the PLBs formation, but the bottom of rhizoid is very important for TDZ absorption.

Key words: Rosa spp., tissue culture, rhizoid, protocorm-like body(PLB), occurrence and development

ZHANG Jian-Fu, BI Ling, CHEN Xiao-Li, GUO Yan-Chao, ZHAO Liang-Jun. Factors Influencing the Occurrence and Development of Rhizoid and Protocorm-Like Body in Rosa spp.[J].Scientia Agricultura Sinica, 2011, 44(22): 4645-4652.

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References

[1]Hsia C, Korban S S. Organogenesis and somatic embryogenesis in callus of Rosa hybrida and Rosa chinensis minima. Plant Cell, Tissue and Organ Culture, 1996, 44:1-6.

[2]Li X Q, Krasnyanski S F, Korban S S. Somatic embryogenesis, secondary somatic embryogenesis, and shoot organogenesis in Rosa. Journal of Plant Physiology, 2002, 159: 313-319.

[3]高莉萍, 包满珠. 月季‘萨蔓莎’愈伤组织的诱导及植株再生. 园艺学报, 2005, 32(3): 534-536.

Gao L P, Bao M Z. Callus induction and pant regeneration of Rosa hybrida ‘Samantha’. Acta Horticulturae Sinica, 2005, 32(3): 534-536.(in Chinese)

[4]张常青, 洪波, 王海琴, 高俊平. 地被月季‘Royal Bassino’高频再生体系的建立. 园艺学报, 2005, 32(6): 1065-1069.

Zhang C Q, Hong B, Wang H Q, Gao J P. High efficiency of in vitro micropropagation and regeneration for ground cover rose ‘Royal Bassino. Acta Horticulturae Sinica, 2005, 32(6): 1065-1069. (in Chinese)

[5]Lloyd D, Roberts A V, Short K C. The induction in vitro of adventitious shoots in Rosa. Euphytica, 1988, 37:31-36.

[6]Rout G R, Debata B K, Das P. Somatic embryogenesis in callus of Rosa hybrida cv. Landora. Plant Cell, Tissue and Organ Culture, 1991, 27: 65-69.

[7]Arene L, Pellegrino C, Gudin S. A comparison of the somaclonal variation level of Rosa hybrida cv. meirutral plants regenerated from callus of direct induction from different vegetative and embryonicts. Euphytica, 1993, 71:83-90.

[8]Dubois L A M, de Vries D P. Preliminary report on the direct regeneration of adventitious buds on leaf explants of in vitro grown glasshouse rose cultivars. Gartenbauwiss, 1995, 60: 249-253.

[9]Ibrahim R, Debergh P C. Factors controlling high efficiency adventitious bud formation and plant regeneration from in vitro leaf explants of roses (Rose hybrida L.). Scientia Horticulturae, 2001, 88: 41-57.

[10]Pati PK, Sharma M, Sood A, Ahuja P S. Direct shoot regeneration from leaf explants of Rosa damascene Mill. In Vitro Cellular & Developmental Biology, 2004, 40(2): 192-195.

[11]任桂芳, 王建红, 冯慧, 李毅, 李燕, 施雪波. 现代月季(Rosa hybrida)叶片植株再生体系的建立. 园艺学报, 2004, 31(4): 533-536.

Ren G F, Wang J H, Feng H, Li Y, Li Y, Shi X B. Establishment of plant regeneration from leaves explants of Rosa hybrida. Acta Horticulturae Sinica, 2004, 31(4): 533-536. (in Chinese)

[12]Joyce C W, Harald T E, Jarmo J H, Ulla T. Somatic embryogenesis and regeneration of flowering plants in rose. Plant Cell Reports, 1990, 9: 456-458.

[13]Noriega C, So¨ndahl M R. Somatic embryogenesis in hybrid tea roses. Nature Biotechnology, 1991, 9:991-993.

[14]Kunitake H, Imamizo H, Mii M. Somatic embryogenesis and plant regeneration from immature seed-derived calli of rugosa rose (Rosa rugosa Thumb). Plant Science, 1993, 90: 187-194.

[15] van der Salm T P M, van der Toorn C J G, ten Cate C H H；Dons H J M. Somatic embryogenesis and shoot regeneration from excised adventitious roots of the rootstock Rosa hybrida L.‘Moneyway’. Plant Cell Reports, 1996, 15: 522-526.

[16]Dohm A, Ludwig C, Nehring K, Debener T. Somatic embryogenesis in roses. Acta Horticulturae, 2001, 547: 341-348.

[17]Sarasan V, Roberts AV, Rout G R. Methyl laurate and 6-benzyladenine promote the germination of somatic embryos of a hybrid rose. Plant Cell Reports, 2001, 20:183-186.

[18]Kim C K, Oh J Y, Chung J D, Burrell A M, Byrne D H. Somatic embryogenesis and plant regeneration from in-vitro-grown leaf explants of rose. HortScience, 2004, 39(6): 1378-1380.

[19]Kim S W, Oh S C, Liu J R. Control of direct and indirect somatic embryogenesis by exogenous growth regulators in immature zygotic embryo cultures of rose. Plant Cell, Tissue and Organ Culture, 2003, 74(1):61-66.

[20]Kamo K, Jones B, Bolar J, Smith, F. Regeneration from long-term embryogenic callus of the Rosa hybrida cv. Kardinal. In Vitro Cellular and Development Biology Plant, 2005, 41:32-36.

[21]Kaur N, Pati P K, Sharma M, Ahuja P S. Somatic embryogenesis from immature zygotic embryos of Rosa bourboniana desp. In Vitro Cellular and Developmental Biology Plant, 2006, 42(2):124-127.

[22]Estabrooks T, Browne R, Dong ZM. 2,4,5-Trichlorophenoxyacetic acid promotes somatic embryogenesis in the rose cultivar ‘Livin’ Easy’ (Rosa spp.). Plant Cell Reports, 2007, 26: 153-160.

[23]Schum A, Hofmann K, Ghalib N, Tawfik A. Factors affecting protoplast isolation and plant regeneration in Rosa spp. Gartenbauwiss, 2001, 66:115-122.

[24]Kim S W, Oh S C, In D S, Liu J R. Plant regeneration of rose (Rosa hybrida) from embryogenic cell-derived protoplasts. Plant Cell, Tissue and Organ Culture, 2003, 73(1): 15-19.

[25]Tian C W, Chen Y, Zhao X A L, Zhao L J. Plant regeneration through protocorm-like bodies induced from rhizoids using leaf explants of Rosa spp. Plant Cell Reports, 2008, 27: 823-831.

[26]田传卫, 尚爱芹, 张建甫, 郭艳, 梁建丽, 赵梁军. 多花蔷薇假珠芽诱导体细胞胚发生及植株高效再生. 园艺学报, 2008, 35(3) :403-408.

Tian C W, Shang A Q, Zhang J F, Guo Y C, Liang J L, Zhao L J. Pseudobulbils induction, somatic embryogenesis and shoot regeneration in Rosa multiflora Thunb. Acta Horticulturae Sinica, 2008, 35(3): 403-408. (in Chinese)

[27]郭艳超, 张倩, 田传卫, 陈菊, 赵梁军. 香水月季类原球茎(PLBs)途径再生植株的研究.中国农业大学学报, 2008, 13(5): 29-34.

Guo Y C, Zhang Q, Tian C W, Chen J, Zhao L J. Regeneration of Rosa odorata via protocorm-like bodies. Journal of China Agriculture University, 2008, 13(5): 29-34. (in Chinese)

[28]郭艳超. 蔷薇属植物类原球茎再生途径的研究[D]. 北京: 中国农业大学，2008.

Guo Y C. Studies of Plant Regeneration Via Protocorm-Like Body（PLB）Way in Rosa spp[D]. Beijing: China Agriculture University, 2008. (in Chinese)

[29]武维华. 植物生理学. 北京：科学出版社, 2008.

Wu W H. Plant Physiology. Beijing: Science Press, 2008. (in Chinese)

[30]Fehér A, Pasternak T P, Dudits D. Transition of somatic plant cells to an embryogenic state. Plant Cell, Tissue and Organ Culture, 2003, 74: 201-228.

[31]师桂英, 宫育红, 徐伟君. 三种生长素对火鹤不定芽生根作用的研究. 甘肃农业大学学报, 2004, 39(3): 281-284.

Shi G Y, Gong Y H, Xu W J. Three tuxins on the root regeneration from adventitious bud of Antharium scherzerium. Journal of Gansu Agricultural University, 2004, 39(3):281-284. (in Chinese)

[32]王艺, 韦小丽. 不同光照对植物生长、生理生化和形态结构影响的研究进展. 山地农业生物学报, 2010, 29(4): 353-359.

Wang Y, Wei X L. Advance on the effects of different light environments on growth, physiological biochemistry and morphostructure of plant. Journal of Mountain Agriculture and Biology, 2010, 29(4): 353-359. (in Chinese)

[33]冯丹丹, 王红梅, 张丽杰, 夏祥友, 沈海龙. 光照、琼脂和碳源对离体培养中水曲柳根尖生长的影响. 植物研究, 2006, 26(4): 424-428.

Feng D D, Wang H M, Zhang L J, Xia X Y, Shen H L. Influence of light, agar and carbon sources to growth of in vitro cultured root tips of Fraxinus mandshurica. Bulletin of Botanical Research, 2006, 26(4): 424- 428.(in Chinese)

[34]王妍, 李宪利, 孙清荣, 周广芳. 泰山酸枣再生不定根特性的比较. 安徽农业科学, 2009, 37(35): 17354-17355, 17464.

Wang Y, Li X L, Sun Q R, Zhou G R. Comparison on characteristic of regeneration adventitious roots in Taishan sour jujube. Journal of Anhui Agricultural Sciences, 2009, 37(35):17354-17355, 17464. (in Chinese)

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