Abstract: Abstract:【Objective】This research aims at developing a plant regeneration system from leaf explants of Diospyros Lotus L. thereby establish a foundation for transformation。【Method】Used tissue culture technique, the conditions for plant regeneration from leaf explants of Diospyros Lotus L. such as leaf segment, inoculate method, basal medium, plant growth regulator and dark period were investigated.【Result】The low segment of leaf with the adaxial surface downwards inoculated on 1/2MS medium containing 0.01 mg•L-1 indole-3-butyric acid(IBA) and 5.0 mg•L-1 zeatin, dark incubated for the first 5 weeks and then transferred to light was the most optimum for shoot regeneration and the shoot percentage and average shoots number per explant were 85.3% and 2.8±0.6 respectively. Regenerated shoots were cut and transferred to 1/2MS medium without any plant growth regulator for rooting. After cultured for one month, the rooting percentage, average roots number and length per shoot reached up to 100%, 5.4 and 3.7 centimeter respectively. After rooting, the regenerated plants were transplant in pots where 98.5% plants survived after one month.【Conclusion】We developed the plant regeneration system from leaf explants of Diospyros Lotus L successfully.

Key words: Persimmon(Diospyros Lotus L.), Leaf segment, In vitro culture, Plant regeneration