Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (07): 1501-1504 .

• RESEARCH NOTES • Previous Articles    

Molecular Cloning and Sequence Analysis of Two cDNA Fragments of Pheromone Binding Protein in Male Beet Armyworm, Spodoptera exigua Hübner

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  1. 南京农业大学农业部病虫监测与治理重点开放实验室
  • Received:2004-09-13 Revised:1900-01-01 Online:2005-07-10 Published:2005-07-10

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Abstract: By reverse transcription-polymerase chain reaction (RT-PCR) method with a pair of degenerate primers, two cDNA fragments of pheromone binding protein（PBP）gene, SexigPBP1 and SexigPBP2, were cloned and sequenced from the antennae of male Spodoptera exigua. SexigPBP1 and SexigPBP2 contained 275 bp and 281 bp, and with deduced amino acid sequences of 92 residues and 94 residues, respectively. The sequence analysis indicated that the deduced amino acid sequences of the cDNA fragments shared high identity with PBPs gene sequences from other previously reported insects.

Key words: Spodoptera exigua, Pheromone binding protein gene, Sequence analysis

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