Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (02): 308-312 .

• PLANT PROTECTION • Previous Articles     Next Articles

Development of Enzyme Immunoassays for Pyrethriods

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  1. 江苏省农业科学院食品质量安全与检测研究中心
  • Received:2004-03-02 Revised:1900-01-01 Online:2005-02-10 Published:2005-02-10

Abstract: 3-phenoxybenzoic acid (PBA) was used as generic hapten in order to generate broad specificity antibody against a group of pyrethriod insecticides. Assay performance was improved by use of a coating antigen, which possessed a six-carbon spacer arm between the hapten and carrier protein. Typical detection limits for PBA was 0.193 ?g·ml-1with an I50 value of 3.707 ?g·ml-1. Different cross-reactions with the closely related pyrethriods,including permethrin, fenpropathrin, cypermetnrin, cyhalothrin and deltamethrin, were observed, with I50 value of 4.066, 4.232,4.286,4.874,8.979 ?g·ml-1, respectively; There was a low cross reactivity for fenvalerate with an I50 value of 77.360 ?g·ml -1. The result developed in this study indicated the antibody was practicable to screen the residues of pyrethriod insecticides.

Key words: Pyrethroids, Pesticide residue, ELISA

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