Scientia Agricultura Sinica ›› 2021, Vol. 54 ›› Issue (6): 1258-1269.doi: 10.3864/j.issn.0578-1752.2021.06.015

• FOOD SCIENCE AND ENGINEERING • Previous Articles     Next Articles

Influence of Exogenous Protein Addition on Whole Wheat Dough Properties and Bread Quality Characteristics

Di WU(),JiaYu WANG,XiaoZhi TANG(),QiuHui HU   

  1. College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing 210023
  • Received:2020-07-04 Accepted:2020-11-13 Online:2021-03-16 Published:2021-03-25
  • Contact: XiaoZhi TANG E-mail:diwu@nufe.edu.cn;warmtxz@njue.edu.cn

Abstract:

【Objective】The influence of two exogenous protein addition on thermomechanical properties, dynamic rheological properties and microstructure of whole wheat dough as well as quality characteristics of bread were investigated. The aim of the research was to provide a basis for further utilization of exogenous protein addition to ameliorate quality of whole wheat food and developing novel products of whole wheat food. 【Method】 Mixolab and dynamic rheometer were used to study the Influence of exogenous protein addition on thermomechanical and rheological properties of whole wheat dough, respectively. The dough microstructure was observed by scanning electron microscope (SEM). Meanwhile, whole wheat bread was prepared and tested to investigate the influence of exogenous protein addition on loaf and volume texture characteristic of bread. 【Result】 With the increasing egg white powder (EW) addition, the water absorption of whole wheat dough decreased, while dough development time and stability time increased. During heating process, peak viscosity of the mixed flour and setback value of starch increased. It was shown from dynamic rheological studies that with the increasing addition of EW, storage modulus and loss modulus of dough consistently decreased, while tan δ of dough increased. From SEM, the dough microstructure has been effectively improved, as the internal pore structure significantly reduced. Furthermore, more continuous and homogeneous dough structure well compensated for the structural damage caused by the bran in whole wheat dough. The specific volume of whole wheat bread increased as well as the bread crumb became loose and soft. The hardness and chewiness decreased, as there was no significant change on springiness, cohesiveness and resilience. Meanwhile, with the increasing sodium caseinate (SC) addition, the water absorption of whole wheat dough and degree of protein weakening increased, while dough development time and stability time first increased and then decreased. During heating process, peak viscosity of the mixed flour decreased while setback value of starch increased. It was shown from dynamic rheological studies that storage modulus and loss modulus of dough increased when SC addition was greater than 1%. From SEM, the dough microstructure gradually densified with the holes and crevices in dough gradually diminished. The specific volume of whole wheat bread first increased and then decreased as well as the bread crumb became relatively dense. It was shown from TPA that the hardness and chewiness gradually increased as well as the cohesiveness and resilience gradually decreased, as there was no significant change on springiness. 【Conclusion】The addition of EW could replace vital wheat gluten to some extent for effectively ameliorating the quality of whole wheat bread, whilst the utilization of SC should consider its higher water absorbing ability.

Key words: exogenous protein, whole wheat bread, thermomechanical properties, rheological properties, quality characteristics

Table 1

Basic component content of raw materials (wet basis) (%)"

淀粉 Starch 蛋白质 Protein 脂肪 Fat 灰分 Ash content 粗纤维 Crude fiber 水分 Water 湿面筋 Wet gluten
65.65±0.15 14.57±0.01 2.02±0.00 1.40±0.05 1.53±0.01 11.63±0.05 30.77±0.07

Table 2

Influence of EW on mixing characteristics of whole wheat dough"

吸水率
Water absorption (%)
形成时间
Formation time (min)
稳定时间
Stabilization time (min)
弱化度
Weakening degree (Nm)
峰值黏度
Peak viscosity (Nm)
回生值Retrogradation value (Nm)
全麦粉 Whole wheat flour 68.17±0.29b 3.63±0.06e 7.12±0.10f 0.64±0.01a 1.49±0.02cd 0.85±0.02d
G 3.0% 71.17±0.29a 4.42±0.13c 8.22±0.06d 0.55±0.02c 1.43±0.02e 0.71±0.01e
EW 0.5% 68.43±0.12b 4.11±0.13d 7.69±0.08e 0.56±0.02bc 1.48±0.02d 0.83±0.02d
EW 1.0% 67.60±0.17c 4.54±0.04c 8.36±0.08d 0.62±0.06a 1.48±0.01d 0.89±0.02c
EW 3.0% 64.33±0.29d 4.70±0.02c 8.76±0.11c 0.60±0.02ab 1.52±0.01b 0.95±0.01b
EW 5.0% 62.47±0.15e 5.64±0.22b 9.19±0.04b 0.57±0.01bc 1.51±0.01bc 1.00±0.02a
EW 7.0% 59.60±0.17f 6.55±0.29a 9.82±0.07a 0.62±0.02a 1.53±0.02a 0.99±0.02a

Table 3

Influence of SC on mixing characteristics of whole wheat dough"

吸水率
Water absorption (%)
形成时间
Formation time (min)
稳定时间
Stabilization time (min)
弱化度
Weakening degree (Nm)
峰值黏度
Peak viscosity
(Nm)
回生值Retrogradation value (Nm)
全麦粉 Whole wheat flour 68.17±0.29f 3.63±0.06e 7.12±0.10e 0.64±0.01b 1.49±0.02a 0.85±0.02c
G 3.0% 71.17±0.29d 4.42±0.13c 8.22±0.06d 0.55±0.02d 1.43±0.01b 0.71±0.01f
SC 0.5% 68.50±0.00f 3.91±0.09d 8.12±0.05d 0.56±0.01d 1.47±0.01a 0.74±0.02e
SC 1.0% 69.43±0.12e 4.44±0.20c 8.66±0.10c 0.57±0.02d 1.43±0.02b 0.79±0.02d
SC 3.0% 72.17±0.15c 4.88±0.03a 9.94±0.07a 0.58±0.02d 1.38±0.01c 0.85±0.02c
SC 5.0% 76.73±0.12b 4.68±0.04b 9.53±0.12b 0.62±0.01c 1.27±0.02d 0.88±0.01b
SC 7.0% 83.27±0.25a 4.29±0.06c 8.77±0.07c 0.66±0.01a 1.17±0.02e 0.93±0.02a

Fig. 1

Influence of EW addition on rheological properties of wheat dough"

Fig. 2

Influence of SC on rheological properties of whole wheat dough"

Fig. 3

Scanning electron micrograph of EW and SC dough a-l represents whole wheat, gluten 3.0%, EW 0.5%, EW 1.0%, EW 3.0%, EW 5.0%, EW 7.0%, SC 0.5%, SC 1.0%, SC 3.0%, SC 5.0%, SC 7.0%, respectively. The same as below"

Table 4

Influence of protein addition on bread specific volume"

比容 Specific capacity (cm3?g-1) 比容 Specific capacity (cm3?g-1)
全麦粉 Whole wheat 3.10±0.02cd 全麦粉 Whole wheat 3.10±0.02b
G 3.0% 3.43±0.05a G 3.0% 3.43±0.05a
EW 0.5% 3.01±0.04d SC 0.5% 2.82±0.01ef
EW 1.0% 3.10±0.01cd SC 1.0% 2.97±0.04cd
EW 3.0% 3.14±0.04bc SC 3.0% 3.03±0.09bc
EW 5.0% 3.20±0.06b SC 5.0% 2.92±0.01de
EW 7.0% 3.21±0.01b SC 7.0% 2.81±0.02f

Fig. 4

Influences of EW and SC on inner pulp structure of whole wheat bread"

Table 5

Influence of EW on texture characteristic of whole wheat bread"

硬度 Hardness (g) 弹性 Elastic 黏聚性 Cohesiveness 咀嚼度 Chewiness 回复性 Resilience
全麦粉 Whole wheat 1913.90±99.03a 0.93±0.01a 0.73±0.04b 1293.40±64.40a 0.27±0.03ab
G 3.0% 1028.20±209.46c 0.89±0.11a 0.79±0.04a 722.22±205.59c 0.30±0.02a
EW 0.5% 2064.40±149.34a 0.86±0.06a 0.70±0.05b 1233.20±119.37a 0.25±0.03b
EW 1.0% 1585.50±65.64b 0.91±0.02a 0.70±0.03b 1005.40±51.52b 0.25±0.01b
EW 3.0% 1628.30±220.53b 0.87±0.01a 0.69±0.01b 971.08±138.54b 0.26±0.01b
EW 5.0% 1472.00±124.09b 0.90±0.01a 0.71±0.02b 927.88±68.20b 0.27±0.01b
EW 7.0% 1063.30±79.68c 0.92±0.02a 0.74±0.02b 718.81±62.03c 0.30±0.02a

Table 6

Influence of SC on texture characteristic of whole wheat bread"

硬度 Hardness (g) 弹性 Elastic 黏聚性 Cohesiveness 咀嚼度 Chewiness 回复性 Resilience
全麦粉 Whole wheat 1913.90±99.03b 0.93±0.01a 0.73±0.04b 1293.40±64.40b 0.27±0.03bc
G 3.0% 1028.20±209.46d 0.89±0.11ab 0.79±0.04a 722.22±205.59d 0.30±0.02a
SC 0.5% 1305.00±171.34c 0.90±0.02ab 0.73±0.01b 857.70±120.46d 0.29±0.01ab
SC 1.0% 1292.70±114.39c 0.86±0.01b 0.74±0.02b 816.62±60.95d 0.28±0.01abc
SC 3.0% 1746.70±217.68b 0.90±0.02ab 0.71±0.02bc 1109.00±113.70c 0.27±0.02cd
SC 5.0% 1943.20±197.12b 0.89±0.02ab 0.70±0.01cd 1203.20±138.87bc 0.25±0.01d
SC 7.0% 2735.30±305.38a 0.88±0.02ab 0.67±0.02d 1624.40±215.64a 0.23±0.01e
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