Scientia Agricultura Sinica ›› 2020, Vol. 53 ›› Issue (14): 2941-2955.doi: 10.3864/j.issn.0578-1752.2020.14.017

• FOOD SCIENCE AND ENGINEERING • Previous Articles     Next Articles

Effects of Xanthan Addition on the Gel Properties and Gel Mechanism of Alkaline-Induced Konjac Glucomannan Gels

LI XiaoFei(),LI PeiYuan,LI AnQi,YU WenYan,GUO Chuo,YANG Xi,GUO YuRong()   

  1. College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119
  • Received:2019-12-02 Accepted:2020-04-29 Online:2020-07-16 Published:2020-08-10
  • Contact: YuRong GUO E-mail:xiaofeili@snnu.edu.cn;yrguo730@snnu.edu.cn

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Abstract:

【Objective】The effects of xanthan addition amounts on the gel properties and gelation mechanism of konjac glucomannan (KGM) gels were investigated, which was expected to provide a theoretical basis and technique references for developing KGM-related gel foods.【Method】The mixed KGM and xanthan systems were prepared by adding different concentrations of xanthan into 2% KGM solution, and then the mixed polysaccharide systems were subjected to heating at 90℃ for 2 h in the presence of 2% Na2CO3. After cooling to room temperature, the composite KGM-xanthan gels were prepared. By determining the gel strength, the effects of deionized water immersion and 2% citric acid immersion as well as freeze-thaw treatment on the composites were investigated. Meanwhile, scanning electron microscope was used to visualize the microstructures of the composite gels, and then the effects of different treatment methods and xanthan addition amounts on the gel structures were also revealed. Moreover, based on rheology, X-ray scattering and thermogravimetric analyses, the gel formation process of the mixed KGM and xanthan systems during heating at hot alkaline condition was explored, and the gelation mechanism was also revealed.【Result】During heating at 90℃ for 2 h, it was found that with the increasing of xanthan addition, the gel strength of the composite gels was decreased, indicating that the presence of xanthan negatively affected the formation of KGM gel network in this process. However, at room temperature, with increasing xanthan content, the gel strength of the composite gels was increased. The higher the xanthan content, the greater the gel strength, indicating that xanthan might strengthen the composite gels during cooling. After dezionized water and 2% citric acid immersion, gel strength decreased, and the gel samples with citric acid immersion showed a more pronounced decreased trend. Besides, after freeze-thaw treatment, KGM gels exhibited significant syneresis effect, with an approximately 50% of syneresis rate. However, after adding xanthan, the syneresis rate of the composite gels was significantly decreased. The higher the xanthan content, the lower the syneresis rate. Rheological test results suggested that during heating at 90℃ for 2 h, with the increasing of xanthan content, the gelling rate of the mixed KGM-xanthan system was decreased, indicating that the addition of xanthan decreased the formation of KGM gel network. Subsequent decreasing temperature sweep results showed that when the temperature decreased from 90℃ to 60℃, the elastic modulus of the composite gels exhibited a decreasing trend. When the temperature was continuously decreased to room temperature, the elastic modulus showed an increasing trend, and the transition temperature was corresponding to 60℃, suggesting that xanthan began to synergistically bind with KGM gel network at this temperature.【Conclusion】The addition of xanthan significantly improved the gel strength of alkali-induced KGM gels at room temperature, and also enhanced the freeze-thaw stability of the gels. This result could provide valuable reference to development of KGM gel-related food.

Key words: konjac glucomannan, xanthan, gel strength, rheological properties, freeze-thaw stability

Table 1

Addition amounts of each component in sample preparation"

编号
No.		 3%魔芋胶
3% KGM (g)		 魔芋胶干重
KGM dry weight (g)		 魔芋胶浓度
KGM concentration (w?v-1)		 5%黄原胶
5% Xanthan (g)		 黄原胶干重
Xanthan dry weight (g)		 黄原胶浓度
Xanthan concentration (w?v-1)		 去离子水
Deionized water (mL)
0 133.3 4 2% 0 0 0 56.7
1 133.3 4 2% 10 0.5 0.25% 46.7
2 133.3 4 2% 20 1 0.5% 36.7
3 133.3 4 2% 30 1.5 0.75% 26.7
4 133.3 4 2% 40 2 1.0% 16.7
5 133.3 4 2% 50 2.5 1.25% 6.7
6 133.3 4 2% 60 3 1.5% 0

Fig. 1

Gel strength of samples at 90℃ and room temperature In the same treatment, different letters indicate significant differences between samples (P<0.05). The same as below"

Fig. 2

Microstructure of samples"

Fig. 3

Gel strength before and after freezing and thawing, the syneresis rate and microstructure of samples after freeze-thawing"

Fig. 4

Gel strength and microstructure of samples after immersion in deionized water and 2% citric acid solution"

Fig. 5

Time sweep curves of sample at 2% alkali concentration and 90℃ A: 2.0% KGM; B: 2.0% KGM+0.25% Xanthan; C: 2.0% KGM+0.5% Xanthan; D: 2.0% KGM+0.75% Xanthan; E: 2.0% KGM+1.0% Xanthan; F: 2.0% Xanthan"

Table 2

Parameters of the fitted equation of first order kinetics of G' of the KGM/Xanthan gels at a fixed KGM concentration (2%, w/v) and different Xanthan concentrations during heating at 90℃"

样品Sample G'sat k R2
2% KGM (Control) 5944.73 3.58×10-4 0.9800
2% KGM+0.25% Xanthan 4613.90 2.42×10-4 0.9918
2% KGM+0.5% Xanthan 3853.96 1.14×10-4 0.9829
2% KGM+0.75% Xanthan 1760.91 0.78×10-4 0.9915
2% KGM+1.0% Xanthan 1247.19 0.54×10-4 0.9963

Fig. 6

Storage modulus and fitting curve of sample during time sweep"

Fig. 7

Change trend of storage modulus of samples during decreasing temperature sweep"

Fig. 8

Frequency sweep curves of sample A: 2.0% KGM; B: 2.0% KGM+0.25% Xanthan; C: 2.0% KGM+0.5% Xanthan; D: 2.0% KGM+0.75% Xanthan; E: 2.0% KGM+1.0% Xanthan; F: 2.0% Xanthan"

Fig. 9

X-ray diffraction of samples"

Fig. 10

Thermogravimetric analysis of samples A: 2.0% KGM; B: 2.0% KGM+0.25% Xanthan; C: 2.0% KGM+0.5% Xanthan; D: 2.0% KGM+0.75% Xanthan; E: 2.0% KGM+1.0% Xanthan; F: 2.0% Xanthan"

[1] 黄明发, 张盛林. 魔芋葡甘聚糖的增稠特性及其在食品中的应用. 中国食品添加剂, 2008(6):127-131.
HUANG M F, ZHANG S L. The thickening characteristics of Konjac Glucomanan and its application in food industry. China Food Additives, 2008(6):127-131. (in Chinese)
[2] 庞杰, 吴春华, 温成荣, 尹娜, 姚闽娜. 魔芋葡甘聚糖凝胶研究进展及其问题. 中国食品学报, 2011,11(9):181-187.
PANG J, WU C H, WEN C R, YIN N, YAO M N. Research advances in Konjac Glucomannan gel and its problem. Journal of Chinese Institute of Food Science and Technology, 2011,11(9):181-187. (in Chinese)
[3] 曾瑞琪, 李苇舟, 赵欣, 张甫生, 郑炯. 魔芋胶-黄原胶复配体系流变学特性及其凝胶形成动力学分析. 食品科学, 2018,39(9):39-46.
ZENG R Q, LI W Z, ZHAO X, ZHANG F S, ZHENG J. Rheological properties and gelation kinetics of Konjac Gum-Xanthan gum mixtures. Food Science, 2018,39(9):39-46. (in Chinese)
[4] 丁保淼, 徐焱春, 熊洪录, 徐振明, 田志宏. 以黄原胶为改良剂的复配胶魔芋豆腐的制备. 食品科技, 2014,39(1):65-69.
DING B M, XU Y C, XIONG H L, XU Z M, TIAN Z H. Preparation of Konjac-Xanthan mixed gum bean curd using Xanthan gum modifier. Food Science and Technology, 2014,39(1):65-69. (in Chinese)
[5] 邓林, 刘延岭. 魔芋胶的流变学及加工特性的研究. 食品工业科技, 2017,38(18):56-59.
DENG L, LIU Y L. Study on rheological and processing characteristics of Konjac gum. Science and Technology of Food Industry, 2017,38(18):56-59. (in Chinese)
[6] MA S P, ZHU P L, WANG M C. Effects of Konjac Glucomannan on pasting and rheological properties of corn starch. Food Hydrocolloids, 2019,89:234-240.
[7] GAO S J, NISHINARI K. Effect of degree of acetylation on gelation of Konjac Glucomannan. Biomacromolecules, 2004,5(1):175-185.
doi: 10.1021/bm034302f pmid: 14715024
[8] 贺丹, 邬应龙. 魔芋-黄原胶复合凝胶溶胀性能的研究. 现代食品科技, 2006,22(3):79-81.
HE D, WU Y L. The study on the swelling properties of Konjac and Xanthan complex gel. Modern Food Science & Technology, 2006,22(3):79-81. (in Chinese)
[9] ANNABLE P, WILLIAMS P A, NISHINARI K. Interaction in Xanthan-Glucomannan mixtures and the influence of electrolyte. Macromolecules, 1994,27:4204-4211.
[10] CHEN Y L, SONG C Z, LV Y K, QIAN X. Konjac glucomannan/ kappa carrageenan interpenetrating network hydrogels with enhanced mechanical strength and excellent self-healing capability. Polymer, 2019,184:121913.
[11] 王元兰, 李忠海, 魏玉. 黄原胶与魔芋胶复配体系的流变特性及影响因素. 中南林业科技大学学报, 2010,30(11):125-128.
WANG Y L, LI Z H, WEI Y. Study of rheological properties and influence factor of solution Xanthan/Konjac gum mixtures. Journal of Central South University of Forestry & Technology, 2010,30(11):125-128. (in Chinese)
[12] KUMAR A, RAO K M, HAN S S. Application of Xanthan gum as polysaccharide in tissue engineering: A review. Carbohydrate Polymers, 2018,180:128-144.
pmid: 29103488
[13] 李肖蒙. 基于丙酮酸含量的变化调控黄原胶与魔芋葡甘聚糖复配协同作用及凝胶特性[D]. 杭州: 浙江工商大学, 2018.
LI X M. Effect of pyruvate group on synergistic effect and gel properties from mixtures of Xanthan and Konjac Glucomannan[D]. Hangzhou: Zhejiang Gongshang University, 2018. ( in Chinese)
[14] LUTFI Z, ALAM F, NAWAB A, HAQ A, HASNAIN A. Effect of NaCl on physicochemical properties of Xanthan gum-Water chestnut starch complexes. International Journal of Biological Macromolecules, 2019,131:557-563.
doi: 10.1016/j.ijbiomac.2019.03.052 pmid: 30880058
[15] 游东宏, 江雪秀. 不同因素对魔芋葡甘聚糖凝胶稳定性的研究. 宁德师范学院学报(自然科学版), 2013,25(4):380-382.
YOU D H, JIANG X X. The study of different factors on the stability of Konjac glucomannan gel. Journal of Ningde Normal University (Natural Science Edition), 2013,25(4):380-382. (in Chinese)
[16] GENEVRO G M, DE MORAES M A, BEPPU M M. Freezing influence on physical properties of glucomannan hydrogels. International Journal of Biological Macromolecules, 2019,128:401-405.
pmid: 30684579
[17] ZHOU Y, JIANG R S, PERKINS W S, CHENG Y Q. Morphology evolution and gelation mechanism of alkali induced Konjac glucomannan hydrogel. Food Chemistry, 2018,269:80-88.
doi: 10.1016/j.foodchem.2018.05.116 pmid: 30100487
[18] 倪俊杰, 曹连鹏, 傅玉颖, 李可馨, 沈亚丽, 王美. 魔芋葡甘聚糖与黄原胶复配凝胶的协同作用及其流变特性. 中国食品学报, 2018,18(10):58-68.
NI J J, CAO L P, FU Y Y, LI K X, SHEN Y L, WANG M. The synergy of mixed gels of Konjac Glucomannan and Xanthan and its rheological behavior. Journal of Chinese Institute of Food Science and Technology, 2018,18(10):58-68. (in Chinese)
[19] YANG X, GONG T, LI D, Li A Q, SUN L J, GUO Y R. Preparation of high viscoelastic emulsion gels based on the synergistic gelation mechanism of Xanthan and Konjac Glucomannan. Carbohydrate polymers, 2019,226:115278.
doi: 10.1016/j.carbpol.2019.115278 pmid: 31582087
[20] YANG X, GONG T, LU Y H, Li A Q, SUN L J, GUO Y R. Compatibility of sodium alginate and konjac glucomannan and their applications in fabricating low-fat mayonnaise-like emulsion gels. Carbohydrate polymers, 2020,229:115468.
doi: 10.1016/j.carbpol.2019.115468 pmid: 31826449
[21] 杨贞. 碱性条件下魔芋胶对小麦淀粉凝胶流变和质构特性的影响[D]. 郑州: 河南农业大学, 2018.
YANG Z. Effect of Konjac Gum on the rheological and texture properties of wheat starch gel under alkaline condition[D]. Zhengzhou: Henan Agricultural University, 2018. (in Chinese)
[22] NING Y J, CUI B, YUAN C, ZOU Y Y, LIU W Z, PAN Y. Effects of Konjac Glucomannan on the rheological, microstructure and digestibility properties of debranched corn starch. Food Hydrocolloids, 2020,100:105342.
[23] GONG T, HOU Y J, YANG X, GUO Y R. Gelation of hydroxyethyl cellulose aqueous solution induced by addition of colloidal silica nanoparticles. International Journal of Biological Macromolecules, 2019,134:547-556.
pmid: 31100393
[24] YANG X, NISAR T, LIANG D, HOU Y J, SUN L J, GUO Y R. Low methoxyl pectin gelation under alkaline conditions and its rheological properties: Using NaOH as a pH regulator. Food Hydrocolloids, 2018,79:560-571.
[25] LI J W, MA J W, CHEN S J, HE J M, HUANG Y D. Characterization of calcium alginate/deacetylated Konjac Glucomannan blend films prepared by Ca2+ crosslinking and deacetylation. Food Hydrocolloids, 2018,82:363-369.
[26] YANG X, HOU Y J, GONG T, SUN L J, XUE J, GUO Y R. Concentration-dependent rheological behavior and gelation mechanism of high acyl gellan aqueous solutions. International Journal of Biological Macromolecules, 2019,131:959-970.
doi: 10.1016/j.ijbiomac.2019.03.137 pmid: 30910680
[27] REINOSO D, MARTIN-ALFONSO M J, LUCKHAM P F, MARTINEZ-BOZA F J. Rheological characterization of Xanthan gum in brine solutions at high temperature. Carbohydrate Polymers, 2019,203:103-109.
doi: 10.1016/j.carbpol.2018.09.034 pmid: 30318193
[28] MAO C F, CHEN C H. A kinetic model of the gelation of Konjac Glucomannan induced by deacetylation. Carbohydrate Polymers, 2017,165:368-375.
doi: 10.1016/j.carbpol.2017.02.078 pmid: 28363561
[29] 侯团伟, 张虹, 毕艳兰, 池娟娟. 食品胶体的凝胶机理及协同作用研究进展. 食品科学, 2014,35(23):347-353.
doi: 10.7506/spkx1002-6630-201423067
HOU T W, ZHANG H, BI Y L, CHI J J. Recent progress in gelation mechanism and synergistic interaction of common gums. Food Science, 2014,35(23):347-353. (in Chinese)
doi: 10.7506/spkx1002-6630-201423067
[30] WILLIAMS P A, CLEGG S M, LANGDON M J, NISHINARI K, PICULELL L. Investigation of the gelation mechanism in κ-carrageenan/ konjac mannan mixtures using differential scanning calorimetry and electron spin resonance spectroscopy. Macromolecules, 1993,26(20):5441-5446.
[31] HUANG L, TAKAHASHI R, KOBAYASHI S, KAWASE T, NISHINARI K. Gelation behavior of native and acetylated Konjac Glucomannan. Biomacromolecules, 2002,3(6):1296-1303.
doi: 10.1021/bm0255995 pmid: 12425668
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