Scientia Agricultura Sinica ›› 2022, Vol. 55 ›› Issue (10): 2038-2046.doi: 10.3864/j.issn.0578-1752.2022.10.013

• FOOD SCIENCE AND ENGINEERING • Previous Articles     Next Articles

Effects of Adding Quinoa Protein Pickering Emulsion on Freeze- Thaw Stability of Fish Surimi Gel

FENG Xiao(),ZHANG Fan,CHEN Ying,CHENG JiaXin,CEN KaiYue,TANG XiaoZhi()   

  1. College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing 210023
  • Received:2021-08-15 Accepted:2022-01-12 Online:2022-05-16 Published:2022-06-02
  • Contact: XiaoZhi TANG E-mail:fengxiao@nufe.edu.cn;9120111004@nufe.edu.cn

Abstract:

【Objective】Based on the freeze-thaw stability of quinoa stabilized Pickering emulsion, it was incorporated evenly into surimi to generate surimi gels in this study, and its feasibility to improve the freeze-thaw stability of fish protein gel was evaluated. This research aimed to prevent the deterioration of fish protein gel, caused by the temperature fluctuation during storage and transportation.【Method】Quinoa protein Pickering emulsion was prepared and distributed in surimi, followed by heating process to generate surimi gels. The surimi gels with different contents of Pickering emulsion and without emulsion were subjected to three freeze-thaw cycles, and then, the texture, color, water and ice crystal distribution and drip loss of surimi gels were measured. 【Result】The quinoa protein Pickering emulsion improved the lightness and whiteness of fish surimi gel, and inhibited the changes of color after freeze-thaw cycles. Meanwhile, the Pickering emulsion addition delayed the changes of hardness and chewiness of surimi gels during freeze-thaw cycles. It was found that quinoa protein Pickering emulsion had no effect on the moisture distribution of surimi gels before freeze-thaw cycles, but significantly increased the proportion of immobile water and decreased free water content in surimi gels after freeze-thaw cycles. Therefore, the drip loss of surimi gel was decreased by emulsion addition. Furthermore, the emulsion addition decreased the diameter of ice crystals formed in surimi gel, reduced the damage to muscle tissues, and decreased the free water content. 【Conclusion】The Quinoa protein Pickering emulsion weakened the adverse impact of freeze-thaw cycles on the color and textural properties, maintained the gel structure, and improved the freeze-thaw stability of fish surimi gel, which maintained its quality and nutritional value. Quinoa protein Pickering emulsion was promising to become an innovative antifreeze to be applied in frozen food.

Key words: quinoa protein, Pickering emulsion, freeze-thaw stability, protein gel

Table 1

The composition of different groups of fish surimi gels with quinoa protein Pickering emulsion addition"

乳液添加量
Emulsion addition amount
鱼糜
Surimi (g)
氯化钠
NaCl (g)
淀粉
Starch (g)
藜麦蛋白Pickering乳液
Quinoa protein Pickering emulsion (g)

Water (g)
0 500 5 10 0 12.50
2.5% 500 5 10 12.5 9.38
5% 500 5 10 25.0 6.25
7.5% 500 5 10 37.5 3.13
10% 500 5 10 50.0 0

Fig. 1

The effects of quinoa protein Pickering emulsion addition on the texture of fish surimi gel before and after freeze-thaw cycles Different capital letters indicate significant differences of textural properties among different groups before and after freeze-thaw cycles (P<0.05). The same as below"

Table 2

The effects of quinoa protein Pickering emulsion addition on the color of fish surimi gel before and after freeze-thaw cycles"

乳液添加比例
Emulsion addition amount (%, w/w)
F0 F3
L* 0 73.69±0.25c 71.95±0.52d
2.5 73.74±0.54c 72.75±0.25cd
5 78.01±2.58ab 74.70±2.55bc
7.5 77.42±0.67b 76.72±0.52ab
10 80.68±2.17a 77.14±0.66a
a* 0 -1.54±0.11c -1.41±0.71a
2.5 -0.79±0.03a -0.98±0.04a
5 -0.78±0.02a -0.83±0.06a
7.5 -1.00±0.06b -1.16±0.10a
10 -0.99±0.08b -1.09±0.04a
b* 0 9.11±0.28c 8.95±0.08b
2.5 9.00±0.12c 9.11±0.14ab
5 9.29±0.34c 9.32±0.59ab
7.5 9.40±0.12ab 9.63±0.39a
10 10.21±0.92a 9.75±0.12a
W 0 72.11±0.33c 70.51±0.48d
2.5 72.23±0.52c 71.24±0.27cd
5 76.09±2.32ab 73.00±2.19bc
7.5 75.52±0.58b 74.77±0.35ab
10 78.08±1.53a 75.12±0.57a

Fig. 2

Transverse relaxation time of fish surimi gels before and after freeze-thaw cycles"

Table 3

The effects of quinoa Pickering emulsion addition on the water distribution of fish surimi gel before and after freeze-thaw cycles"

乳液添加比例
Emulsion addition amount (%, w/w)
F0 F3
结合水
Bound water (%)
0 1.70±0.33ab 0.77±0.43a
2.5 1.86±0.28ab 1.21±0.76ab
5 1.40±0.34ab 0.86±0.94ab
7.5 0.96±0.21ab 2.28±0.67b
10 0.99±0.45ab 2.21±1.76ab
不易流动水
Immobilized water
(%)
0 92.33±0.67e 79.71±0.25b
2.5 93.04±0.68e 76.50±1.84a
5 92.69±0.69e 81.73±2.05c
7.5 93.76±0.35e 83.46±1.46cd
10 93.82±0.27e 83.96±1.55d
自由水
Free water
(%)
0 5.98±0.87a 19.53±0.45d
2.5 5.10±0.65a 22.29±1.77e
5 5.91±0.43a 17.40±1.20c
7.5 5.28±0.56a 14.26±1.06b
10 5.20±0.18a 13.83±0.51b

Fig. 3

The effects of quinoa Pickering emulsion addition on the drip loss of fish surimi gel after freeze-thaw cycles"

Fig. 4

The fluorescence microscope images of ice crystal distribution in surimi gels before and after freeze-thaw cycles The scale bar in the graph is 5 µm"

Table 4

The effects of quinoa Pickering emulsion addition on the ice crystal distribution of fish surimi gel"

组别
Group
冰晶参数 Parameters for ice crystals
直径 Diameter (μm) 数目 Number 总面积 Total area (μm2) 面积百分比 Area percentage (%)
0 8.79±0.05a 19.33±0.58a 242.50±2.81a 40.36±0.47a
2.5% 6.84±0.81bc 25.33±2.08a 148.06±4.84bc 26.44±7.29bc
5.0% 7.29±0.83b 21.67±7.57a 168.31±36.70b 27.53±6.00b
7.5% 5.03±0.23d 23.33±8.96a 79.63±7.38d 13.73±2.13d
10% 5.91±0.66cd 28.67±3.06a 110.43±25.06cd 18.22±4.13cd
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