整合微生物组菌剂的提出、研发与应用

doi:10.3864/j.issn.0578-1752.2019.14.006

Abstract

Abstract:

【Objective】Using microbial fermentation bed as fermentation tank, pig manure nitrogen was continuously added to achieve aerobic fermentation in medium temperature. Microbiome with high bacterial content identified by metagenomic technology was named as integrated microbiome. Integrated microbiome agent (IMA) was produced as plant disease biocontrol agent.【Method】The production process is as follows: material preparation→fermentation→nitrogen feeding→aerobic fermentation control→product processing→product packaging, etc. The fully manufacturing processes of IMAs were as follows: the litters consisting of soybean cake powders (30%) and Pleurotus eryngii mushroom substrates (70%) were added onto the pig microbial fermentation bed that had been used for more than one year with 10 cm thick; the aerobic fermentation was conducted for 20 days by ploughing the litters mixing with pig manures one time per day; then, the upper 20 cm litters were removed to produce IMAs by drying, crushing, screening and packaging.【Result】The physicochemical properties of the IMA products were moisture content 29.74%, pH 7.56, organic matter content 44.46%, total nitrogen content 2.23%, humic acid content 11.20%, crude fiber content 14.06%, and the amount of bacteria was 145×10 ⁸cfu/g. The results of metagenomic determination showed that the average number of the short sequences (reads) was 99 701.75 in the IMA samples, and the IMA samples contained 39 phyla, 96 classes, 189 orders, 383 families, 786 genera, and 1 281 species of bacteria per gram. Among bacteria, 46 Bacillus-like species were detected, and 9 species were the new records in China, namely: (1) Bacillus aerophilus, (2) Bacillus eiseniae, (3) Bacillus filamentosus, (4) Bacillus kochii, (5) Bacillus rhizosphaerae, (6) Lysinibacillus macroides, (7) Oceanobacillus caeni, (8) Ornithinibacillus scapharcae, (9) Virgibacillus oceani. Importantly, no bacterial pathogen of pig was found in the IMA products. The total content of the cultural Bacillus-like species was 2.062×10 ⁸cfu/g, and they accounted for 1.42% of total bacterial abundance in the metagenomic data. Therefore, the total amount of effective bacteria was estimated to be 145×10 ⁸cfu/g in the IMA products based on the cultural Bacillus-like species content and total abundance of metagenomic detecting. The germination rate of mung bean in the IMA group was 96.67%, which was not significantly different from that in the clear water control group (P>0.05), but the radicle length was 58.08% higher than that in the control group. When the seedling-raising substrate was mixed with 5%-10% of IMAs, the emergence rate and plant height of tomato were increased by 3.0% and 25.1%, respectively, and the corrected control efficacy on tomato bacterial wilt was 79.41%. According to the national quality standards for the bio-organic fertilizers (NY884-2012), those of the IMA products were initially set as followings: organic matter content≥40%, moisture content≤30%, pH 5.5-7.5, fecal coliform count≤100 cfu/g, mortality of ascarid egg>95%, expiry date>6 months; The contents of heavy metals: As<15 mg·kg ^-1, Cd≤15 mg·kg ^-1, Pb ≤15 mg·kg ^-1, Cr≤15 mg·kg ^-1, and Hg≤15 mg·kg ^-1; The amount of living bacteria was adjusted to the total living bacteria amount≥30×10 ⁸cfu/g, Bacillus-like species amount≥2×10 ⁸cfu/g.【Conclusion】The concept and product technical standard of the integrated microbiome agents (IMAs) were proposed. The IMA can promote the growth of seed root and have a good control efficacy on tomato bacterial wilt.

Key words: integrated microbiome agent (IMA), bio-organic fertilizer, mung bean, germination rate, radicle length, growth rate, bacterial wilt, control efficacy

Bo LIU,QianQian CHEN,JiePing WANG,ChuanQing RUAN,YanPing CHEN,JiangPing XIA,JianMei CHE,Zheng CHEN,ZhiZhen PAN,Xiao WEN,YuJing ZHU,HaiFeng ZHANG,XueFang ZHENG. Proposition, Development and Application of the Integrated Microbiome Agent (IMA)[J].Scientia Agricultura Sinica, 2019, 52(14): 2450-2467.

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Figures/Tables 11

Fig. 1

Table 1

Table 2

Identification of Bacillus-like species in the product of the integrated microbiome agent"

菌落编号 Colony number	菌株编号 Strain number	标准菌株学名 Name of Bacillus-like species	16S rDNA相似度 16S rDNA similarity (%)
1.	FJAT-46225	[1] 芽孢杆菌Bacillus sp.	97.13
2.	FJAT-46301	[2] 嗜气芽孢杆菌Bacillus aerophilus 28K^T	100.00
3.	FJAT-46217	[2] 嗜气芽孢杆菌Bacillus aerophilus 28K^T	100.00
4.	FJAT-46253	[3] 高地芽孢杆菌Bacillus altitudinis 41KF2b^T	100.00
5.	FJAT-46318	[4] 解淀粉芽孢杆菌Bacillus amyloliquefaciens FZB42^T	99.72
6.	FJAT-46213	[5] 蜡样芽孢杆菌Bacillus cereus ATCC 14579^T	99.70
7.	FJAT-46201	[5] 蜡样芽孢杆菌Bacillus cereus ATCC 14579^T	99.64
8.	FJAT-46307	[6] 阿氏芽孢杆菌Bacillus aryabhattai B8W22^T	100.00
9.	FJAT-46281		97.89
10.	FJAT-46278		100.00
11.	FJAT-46272		100.00
12.	FJAT-46223		100.00
13.	FJAT-46186		99.86
14.	FJAT-46270	[7] 克劳氏芽孢杆菌Bacillus clausii DSM 8716^T	99.50
15.	FJAT-46194	[7] 克劳氏芽孢杆菌Bacillus clausii DSM 8716^T	99.20
菌落编号 Colony number	菌株编号 Strain number	标准菌株学名 Name of Bacillus-like species	16S rDNA相似度 16S rDNA similarity (%)
16.	FJAT-46263	[8] 蚯蚓芽孢杆菌Bacillus eiseniae A1-2^T	99.58
17.	FJAT-46178	[8] 蚯蚓芽孢杆菌Bacillus eiseniae A1-2^T	99.72
18.	FJAT-46289	[9] 丝状芽孢杆菌Bacillus filamentosus SGD-14^T	100.00
19.	FJAT-46204	[9] 丝状芽孢杆菌Bacillus filamentosus SGD-14^T	100.00
20.	FJAT-46297	[10] 柯赫芽孢杆菌Bacillus kochii WCC 4582^T	99.93
21.	FJAT-46258		99.79
22.	FJAT-46246		100.00
23.	FJAT-46230		99.65
24.	FJAT-46221		99.65
25.	FJAT-46208		99.72
26.	FJAT-46202		99.86
27.	FJAT-46176		98.86
28.	FJAT-46175		99.72
29.	FJAT-46313	[11] 地衣芽孢杆菌Bacillus licheniformis ATCC 14580^T	99.10
30.	FJAT-46312		99.30
31.	FJAT-46296		99.70
32.	FJAT-46271		99.37
33.	FJAT-46268		99.23
34.	FJAT-46266		99.37
35.	FJAT-46260		99.40
36.	FJAT-46244		99.72
37.	FJAT-46239		99.30
38.	FJAT-46237		97.70
39.	FJAT-46235		99.16
40.	FJAT-46233		99.37
41.	FJAT-46218		98.30
42.	FJAT-46215		99.65
43.	FJAT-46212		97.50
44.	FJAT-46203		99.93
45.	FJAT-46200		99.72
46.	FJAT-46196		99.79
47.	FJAT-46188		99.60
48.	FJAT-46182		99.70
49.	FJAT-46180		99.60
50.	FJAT-46173		99.23
51.	FJAT-46197	[12] 甲基营养型芽孢杆菌Bacillus methylotrophicus KACC 13105^T	100.00
52.	FJAT-46274	[13] 根际芽孢杆菌Bacillus rhizosphaerae SC-N012^T	99.09
53.	FJAT-46174	[14] 沙福芽孢杆菌Bacillus safensis FO-36b^T	100.00
54.	FJAT-46311	[15] 索诺拉沙漠芽孢杆菌Bacillus sonorensis NBRC 101234^T	99.44
55.	FJAT-46255		99.30
56.	FJAT-46199		99.37
57.	FJAT-46177		99.44
菌落编号 Colony number	菌株编号 Strain number	标准菌株学名 Name of Bacillus-like species	16S rDNA相似度 16S rDNA similarity (%)
58.	FJAT-46316	[16] 枯草芽孢杆菌茵氏亚种Bacillus subtilis subsp. inaquosorum KCTC 13429^T	99.72
59.	FJAT-46298		99.93
60.	FJAT-46295		99.93
61.	FJAT-46279		99.93
62.	FJAT-46259		99.93
63.	FJAT-46252		99.79
64.	FJAT-46251		99.72
65.	FJAT-46248		99.93
66.	FJAT-46247		99.93
67.	FJAT-46242		99.79
68.	FJAT-46238		99.93
69.	FJAT-46231		99.93
70.	FJAT-46229		99.93
71.	FJAT-46220		99.37
72.	FJAT-46211		99.93
73.	FJAT-46206		99.93
74.	FJAT-46185		99.79
75.	FJAT-46184		99.93
76.	FJAT-46262	[17] 枯草芽孢杆菌枯草亚种Bacillus subtilis subsp. subtilis NCIB 3610^T	99.93
77.	FJAT-46241		99.93
78.	FJAT-46234		99.93
79.	FJAT-46219		99.93
80.	FJAT-46214		99.93
81.	FJAT-46190		99.93
82.	FJAT-46179		99.93
83.	FJAT-46314	[18] 特基拉芽孢杆菌Bacillus tequilensis KCTC 13622^T	100.00
84.	FJAT-46257		100.00
85.	FJAT-46250		99.79
86.	FJAT-46216		100.00
87.	FJAT-46191		99.86
88.	FJAT-46249	[19] 人参哈格瓦氏菌Bhargavaea ginsengige 14^T	99.79
89.	FJAT-46227		99.93
90.	FJAT-46224		99.93
91.	FJAT-46192		99.79
92.	FJAT-46276	[20] 赖氨酸芽孢杆菌Lysinibacillus sp.	97.76
93.	FJAT-46275	[21] 淤泥大洋芽孢杆菌Oceanobacillus caeni S-11^T	99.30
94.	FJAT-46254	[22] 拾蛤鸟氨酸芽孢杆菌Ornithinibacillus scapharcae TW25^T	98.47
95.	FJAT-46303	[23] 盐反硝化枝芽孢杆菌Virgibacillus halodenitrificans DSM10037^T	99.79
96.	FJAT-46195	[23] 盐反硝化枝芽孢杆菌Virgibacillus halodenitrificans DSM10037^T	99.86
97.	FJAT-46309	[24] 海洋枝芽孢杆菌Virgibacillus oceani MY11^T	98.70
98.	FJAT-46232		98.30
99.	FJAT-46198		98.29
100.	FJAT-46222	[25] 盐湖枝芽孢杆菌Virgibacillus salinus XH-22^T	98.48

Table 2

Table 3

Table 4

Fig. 2

Table 5

The species richness of Bacillus-like species in the integrated microbiome agent"

种名 Species name	丰度Species richness (%)							平均值 Average (%)
种名 Species name	g1	g2	g3	g4	g5	g6	g7	平均值 Average (%)
[1] 穿琼脂氨芽孢杆菌 Ammoniibacillus agariperforans	0.0000	0.0015	0.0000	0.0000	0.0000	0.0162	0.0000	0.0025
[2] 兼性芽孢杆菌Amphibacillus sp.	0.0000	0.0000	0.0060	0.0160	0.0000	0.0036	0.0018	0.0039
[3] 解硫胺素芽孢杆菌Aneurinibacillus sp.	0.0000	0.0008	0.0048	0.0000	0.0000	0.0009	0.0000	0.0009
[4] 产氮芽孢杆菌Bacillus azotoformans	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000
[5] 草坪芽孢杆菌 Bacillus graminis	0.0010	0.0023	0.0036	0.0015	0.0008	0.0018	0.0000	0.0016
[6] 土地芽孢杆菌Bacillus humi	0.0010	0.0008	0.0084	0.0015	0.0025	0.0234	0.0009	0.0055
[7] 深层芽孢杆菌 Bacillus infernus	0.0039	0.0054	0.0144	0.0036	0.0025	0.0036	0.0343	0.0097
[8] 芽孢杆菌1 Bacillus sp.1	0.0000	0.0108	0.0000	0.0000	0.0659	0.0009	0.0009	0.0112
[9] 芽孢杆菌2 Bacillus sp.2	0.0614	0.2079	1.2836	0.4665	0.1327	0.7332	0.4033	0.4698
[10] 热乳芽孢杆菌Bacillus thermolactis	0.0010	0.0062	0.0120	0.0036	0.0025	0.0108	0.0158	0.0074
[11] 热碱芽孢杆菌CaldalkaLibacillus sp.	0.0000	0.0015	0.0000	0.0000	0.0008	0.0099	0.0018	0.0020
[12] 脱硫芽孢杆菌 Desulfuribacillus sp.	0.0088	0.0000	0.0000	0.0000	0.0008	0.0000	0.0000	0.0014
[13] 地芽孢杆菌1 Geobacillus sp.1	0.0000	0.0054	0.0012	0.0000	0.0017	0.3603	0.0018	0.0529
[14] 嗜热嗜脂肪地芽孢杆菌Geobacillus stearothermophilus	0.0000	0.0015	0.0012	0.0029	0.0017	0.0018	0.0009	0.0014
[15] 纤细芽孢杆菌Gracilibacillus sp.	0.0029	0.0100	0.0036	0.0058	0.0017	0.3423	0.0326	0.0570
[16] 喜盐芽孢杆菌Halobacillus sp.	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000
[17] 嗜盐盐乳芽孢杆菌 Halolactibacillus halophilus	0.0556	0.0031	0.0108	0.1844	0.0225	0.0117	0.0123	0.0429
[18] 盐乳芽孢杆菌Halothiobacillus sp.	0.0010	0.0000	0.0012	0.0146	0.0000	0.0018	0.0044	0.0033
[19] 食淀粉乳杆菌Lactobacillus amylovorus	0.1950	0.0254	0.0588	0.0722	0.0250	1.0145	0.1101	0.2144
[20] 动物乳杆菌Lactobacillus animalis	0.0010	0.0023	0.0000	0.0036	0.0000	0.0036	0.0000	0.0015
[21] 乳杆菌新种 Lactobacillus equicursoris	0.0010	0.0062	0.0000	0.0007	0.0000	0.0584	0.0044	0.0101
[22] 粘膜乳杆菌Lactobacillus mucosae	0.0000	0.0008	0.0000	0.0015	0.0000	0.0306	0.0000	0.0047
[23] 清酒乳杆菌Lactobacillus sakei	0.0000	0.0008	0.0000	0.0000	0.0000	0.0000	0.0000	0.0001
[24] 乳杆菌1 Lactobacillus sp.1	0.3246	0.0477	0.1465	0.4497	0.0209	0.6676	0.1330	0.2557
[25] 小鳟鱼大洋芽孢杆菌Oceanobacillus oncorhynchi	0.0000	0.0008	0.0000	0.0000	0.0000	0.0000	0.0000	0.0001
[26] 大洋芽孢杆菌1 Oceanobacillus sp.1	0.0000	0.0000	0.0000	0.0066	0.0000	0.0045	0.0026	0.0020
[27] 大洋芽孢杆菌2 Oceanobacillus sp.2	0.0049	0.0023	0.0024	0.0117	0.0000	0.0521	0.0070	0.0115
[28] 加利福尼亚鸟氨酸芽孢杆菌 Ornithinibacillus californiensis	0.0049	0.0154	0.0144	0.0117	0.0008	0.0279	0.0317	0.0152
[29] 鸟氨酸芽孢杆菌DX-3 Ornithinibacillus sp. DX-3	0.0019	0.0008	0.0060	0.0022	0.0025	0.0350	0.0352	0.0120
[30] 鸟氨酸芽孢杆菌GD05 Ornithinibacillus sp. GD05	0.0000	0.0008	0.0000	0.0000	0.0000	0.0000	0.0026	0.0005
[31] 鸟氨酸芽孢杆菌HME7715 Ornithinibacillus sp. HME7715	0.0000	0.0000	0.0000	0.0000	0.0000	0.0180	0.0000	0.0026
[32] 类芽孢杆菌1 Paenibacillus sp.1	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0062	0.0009
[33] 类芽孢杆菌2 Paenibacillus sp.2	0.0078	0.0331	0.0096	0.0204	0.0150	0.0494	0.0819	0.0310
[34] 少盐芽孢杆菌1 Paucisalibacillus sp.1	0.0000	0.0000	0.0024	0.0000	0.0008	0.0009	0.0009	0.0007
[35] 少盐芽孢杆菌2 Paucisalibacillus sp.2	0.0029	0.0115	0.0060	0.0087	0.0025	0.0099	0.0123	0.0077
种名 Species name	丰度Species richness (%)							平均值 Average (%)
种名 Species name	g1	g2	g3	g4	g5	g6	g7	平均值 Average (%)
[36] 少盐芽孢杆菌3 Paucisalibacillus sp.3	0.0049	0.0000	0.0072	0.0095	0.0025	0.0584	0.0088	0.0130
[37] 厚胞鲁梅尔芽孢杆菌Rummeliibacillus pycnus	0.0146	0.0062	0.0048	0.0073	0.0000	0.0045	0.0018	0.0056
[38] 森林土壤芽孢杆菌Solibacillus silvestris	0.0049	0.0000	0.0000	0.0000	0.0000	0.0009	0.0000	0.0008
[39] 硫化芽孢杆菌Sulfobacillus sp.	0.0000	0.0023	0.0024	0.0000	0.0000	0.0000	0.0018	0.0009
[40] 热芽孢杆菌1 Thermobacillus sp.1	0.0000	0.0000	0.0000	0.0000	0.0008	0.0000	0.0114	0.0018
[41] 热芽孢杆菌2 Thermobacillus sp.2	0.0000	0.0069	0.0024	0.0000	0.0008	0.0036	0.0018	0.0022
[42] 热芽孢杆菌3 Thermobacillus sp.3	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000
[43] 热生肿块芽孢杆菌 Tuberibacillus calidus	0.0000	0.0008	0.0024	0.0029	0.0000	0.0009	0.0106	0.0025
[44] 肿块芽孢杆菌Tuberibacillus sp.	0.0000	0.0000	0.0216	0.8951	0.0000	0.0000	0.0379	0.1364
[45] 尿素芽孢杆菌Ureibacillus sp.	0.0058	0.0023	0.0000	0.0000	0.0008	0.0054	0.0141	0.0041
[46] 火山芽孢杆菌Vulcanibacillus sp.	0.0273	0.0008	0.0000	0.0153	0.0442	0.0027	0.0009	0.0130
总和Total	0.7381	0.4244	1.6377	2.2195	0.3527	3.5710	1.0278	1.4244

Table 5

Table 6

Table 7

Table 8

Fig. 3

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样品序号 Sample number	水分含量 Water content (%)	pH	有机质 Organic material (%)	全氮 Total nitrogen (%)	腐殖酸 Humic acid (%)	粗纤维 Coarse fiber (%)
g1	31.60	9.20	45.30	2.42	11.10	11.40
g2	27.60	7.10	38.90	2.31	9.31	11.30
g3	27.70	6.30	45.90	2.00	9.88	17.80
g4	33.60	6.20	45.10	2.10	11.40	14.80
g5	31.40	9.50	42.20	2.08	12.70	12.80
g6	28.70	8.10	45.30	2.62	12.90	13.00
g7	27.60	6.50	48.50	2.09	11.10	17.30
平均值Average	29.74	7.56	44.46	2.23	11.20	14.06

样本 Sample	短序列 Reads	种类 OTUs	在0.97的相似度下Under the similarity of 0.97
样本 Sample	短序列 Reads	种类 OTUs	Ace指数 Ace index	Chao指数 Chao index	测序深度指数 Coverage index	香农多样性指数 Shannon index	辛普森优势度指数 Simpson index
g1	102575	1608	1824 (1779, 1882)	1879 (1809, 1973)	0.997114	5.14 (5.13, 5.15)	0.0212 (0.0208, 0.0216)
g2	129891	1698	1922 (1875, 1981)	1973 (1902, 2068)	0.997721	5.55 (5.54, 5.55)	0.0101 (0.01, 0.0103)
g3	83279	1255	1604 (1536, 1689)	1592 (1511, 1700)	0.995845	4.68 (4.67, 4.70)	0.0252 (0.0248, 0.0255)
g4	137195	1192	1381 (1337, 1438)	1421 (1356, 1512)	0.998287	4.85 (4.85, 4.86)	0.0187 (0.0185, 0.0189)
g5	119811	1711	1997 (1942, 2067)	2047 (1966, 2153)	0.997087	5.11 (5.10, 5.12)	0.0255 (0.0251, 0.026)
g6	111290	1257	1566 (1503, 1646)	1574 (1493, 1683)	0.997232	4.96 (4.95, 4.97)	0.0153 (0.0151, 0.0155)
g7	113573	1564	1791 (1744, 1851)	1817 (1752, 1904)	0.997385	5.19 (5.17, 5.20)	0.0159 (0.0157, 0.0161)
平均值Average	99701.75	1469.29	1726.42	1757.57	0.997239	5.0686	0.0188

处理 Treatment	发芽率 Germination rate (%)	胚根长Radical length (RL, cm)		发芽指数Germination index (GI)		活力指数Vigor index (VI)
处理 Treatment	发芽率 Germination rate (%)	RL	比值Ratio	GI	比值Ratio	VI	比值Ratio
整合菌剂IMA	96.67±2.11a	2.64±0.77A	1.5808	57.67a	0.9885	152.25±13.29A	1.5626
清水对照CK	98.33±1.53a	1.67±0.23B	1.0000	58.34a	1.0000	97.43±10.67B	1.0000

处理组 Treatment	处理编号 Treatment number	整合菌剂含量IMA percent (%)	番茄种苗生长状况Growth of tomato seedlings
处理组 Treatment	处理编号 Treatment number	整合菌剂含量IMA percent (%)	出苗率 Emergence rate (%)	根长 Root length (cm)	株高 Plant height (cm)	茎粗 Stem diameter (cm)
高含量组 High content group	处理1 Treatment 1	30	9.04	0.70	1.50	0.70
	处理2 Treatment 2	25	46.10	1.45	6.23	1.05
	平均值Average	—	27.57c	1.08b	3.87c	0.88b
中含量组 Middle content group	处理3 Treatment 3	20	85.30	4.45	9.78	1.56
	处理4 Treatment 4	15	86.03	4.85	9.67	1.45
	平均值Average	—	85.67b	4.65a	9.73b	1.51a
低含量组 Low content group	处理5 Treatment 5	10	89.50	4.73	10.67	1.49
	处理6 Treatment 6	5	88.90	4.62	11.92	1.51
	平均值Average	—	89.20a	4.68a	11.30a	1.50a
空白对照CK	对照 CK	0	86.20b	4.68a	9.03b	1.40a

处理组 Treatment	处理编号 Treatment number	整合菌剂含量 IMA percent (%)	出苗30 d后接种青枯病原菌液 Inoculation of R. solanacearum 30 days after emergence (10⁶ cfu/mL)			10 d校正防效Corrected control efficacy of 10 d (%)
处理组 Treatment	处理编号 Treatment number	整合菌剂含量 IMA percent (%)	4 d后发病率 Incidence after 4 d (%)	7 d后发病率 Incidence after 7 d (%)	10 d后发病率 Incidence after 10 d (%)	10 d校正防效Corrected control efficacy of 10 d (%)
高含量组 High content group	处理1 Treatment 1	30	—	—	—
	处理2 Treatment 2	25	6.25	23.00	66.67
	平均值Average	—	6.25b	23.00a	66.67a	17.88
中含量组 Middle content group	处理3 Treatment 3	20	6.47	13.33	32.40
	处理4 Treatment 4	15	8.33	16.57	28.50
	平均值Average	—	7.40ab	14.95b	30.45ab	62.49
低含量组 Low content group	处理5 Treatment 5	10	6.67	14.47	18.30
	处理6 Treatment 6	5	6.67	13.33	15.14
	平均值Average	—	6.67b	13.90b	16.72b	79.41
空白对照CK	对照 CK	0	11.11a	33.33a	88.89a	—

Proposition, Development and Application of the Integrated Microbiome Agent (IMA)

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样本编号 Sample number	芽孢杆菌活菌计数Colony forming unit of Bacillus species (×10⁷cfu/g)			平均值 Average (×10⁷cfu/g)
样本编号 Sample number	重复I Rep1	重复II Rep2	重复III Rep3	平均值 Average (×10⁷cfu/g)
g1	20	31	29	26.67
g2	23	18	22	21.00
g3	19	16	18	17.67
g4	26	18	21	21.67
g5	18	19	18	18.33
g6	21	15	19	18.33
g7	19	22	21	20.67
平均值 Average (×10⁷cfu/g)	20.85	19.85	21.14	20.62

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