硫氧还蛋白相互作用蛋白与卵母细胞葡萄糖代谢关系的研究进展

doi:10.3864/j.issn.0578-1752.2015.S.009

Abstract

Abstract: Thioredoxin-interacting protein (TXNIP) is a member of the thioredoxin (Trx) super family of proteins, which is the only α-arrestin family member that binds to and negatively regulates Trx. TXNIP can inhibit the function of Trx system by binding Trx, and act as a competitive inhibitor to remove Trx from apoptosis signal-regulating kinase 1 (ASK1). TXNIP is closely related to the cellular reduction-oxidation (redox) state and plays an important role in cell apoptosis. TXNIP is a critical regulator of glucose metabolism. High glucose recruits carbohydrate response element-binding protein (ChREBP) and the transcription factor MondoA:Mlx to the TXNIP promoter and mediates glucose-induced TXNIP expression. Histone deacetylase 1(HDAC1), nuclear factor Y (NF-Y) and the forkhead boxO1 transcription factor (FOXO1) have been reported to regulate the transcription of TXNIP. TXNIP can also inhibit cell proliferation by repressing cyclin-dependent kinase activity. Some studies have found that elevated TXNIP levels induce beta cell apoptosis and inhibit insulin signaling pathway, then promote the occurrence of diabetes and the associated medical comorbidities. Clinical data suggest that hyperglycemia induced by diabetes seriously affects metabolic activity and quality in oocytes. Glucose is the substrate for many cellular functions that can regulate the process of meiosis, and is metabolised by glycolysis pathway, the pentose phosphate pathway, the hexosamine biosynthesis pathway and the polyol pathway. Recent studies have revealed that upon specific depletion of TXNIP, most of oocytes were arrested at metaphate I (MI) stage, which exhibited disturbed actin networking and upregulated glucose uptake and lactate production, indicating that TXNIP is an important regulator of glucose metabolism in oocytes. In summary, it may exist complex regulatory relationships between TXNIP and glucose metabolism in oocytes. TXNIP may be a critical target mediating glucose metabolism in oocytes, and further study will provide important theoretical reference for abnormal metabolic studies. Hence, the structure and regulatory mechanism of TNXIP, pathways of glucose metabolism in oocytes and the relationship between TXNIP and glucose metabolism in oocytes are reviewed in this paper.

Key words: TXNIP, oocyte, glucose, metabolism

PANG Yun-wei, SUN Ye-qing, DU Wei-hua, HAO Hai-sheng, ZHAO Xue-ming, WANG Dong, ZHU Hua-bin. Research Advance on the Relationship Between Thioredoxin- Interacting Protein and Glucose Metabolism in Oocytes[J].Scientia Agricultura Sinica, 2015, 48(S): 75-85.

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