Abstract: 【Objective】 Aflatoxin contaminates agricultural product severely, threatens health and life of people and livestocks. It is one of the major focus issues and attracts both governmental and social concerns. Thus, it is required to establish a rapid and sensitive determination method for aflatoxin. High specific and sensitive monoclonal antibody against aflatoxin has been developed. In this study, using the as-prepared monoclonal antibody against aflatoxin, the aim is to establish time-resolved fluorescence immunochromatography for afaltoxin determination in agricultural product, in order to provide technical support for agricultural product quality supervision and risk assessment. 【Method】 Herein, monoclonal antibody against aflatoxin was coupled with emulsion europium for labeling. With the home-made time-resolved fluorescence immunochromatographic strip, aflatoxin could be determined quantitatively, by using the signal value ratio of test line to control line and natural logarithm of concentration in standard aflatoxin solution. Regarding to various agricultural product samples (as peanut, rice and vegetable oil), an integration technology was developed that combined grinding and homogenization in one step. The methodological evaluation was conducted, in which aflatoxin in real agricultural products such as peanut, rice and vegetable oil. Moreover, these results via time-resolved fluorescence immunochromatography were compared with those via HPLC method. 【Result】 Results showed a detecting limit of 0.3 μg·kg^-1, a liner range of 0.8-25, 0.8-15, 0.8-30 μg·kg^-1, for peanut, rice, plant oil, respectively. The standard curves were recorded as y=0.238x+0.654 (R²=0.992), y=0.321x+0.811 (R²=0.990), and y=0.146x+0.173 (R²=0.993), for peanut, rice, and plant oil, respectively. Recoveries from inner accuracy and precision were between 81.0% and 113.0% with RSD of 7.2%-14.2%, while recoveries from inter accuracy and precision were of 75.8%-114.9% with RSD of 7.7%-15.3%. It was suggested that this method allowed considerable inner- and inter- accuracy and precision, It was found to be the relative error below 10% between results via time-resolved fluorescence immunochromatography and those with HPLC, which suggesting that a high degree between time-resolved fluorescence immunochromatographic method and industry standard method.【Conclusion】Results showed that TRFIA possesses high sensitivity, wide liner range, high repeatability and stability, thus it is suitable for rapid assay in China and poses wide application prospect.

Key words: aflatoxin, time-resolved fluorescence immunochromatography, agricultural product, rapid assay