可可链霉菌182-2活性代谢产物对烟草赤星病菌的作用

doi:10.3864/j.issn.0578-1752.2013.23.009

Abstract

Abstract: 【Objective】 The objective of this study is to understand the antifungal activity and mechanism of KA08 produced by Streptomyces cacaoi strain 182-2 against Alternaria alternata so as to provide a scientific basis for further product development and application.【Method】Estimation of mycelia wet weight and spore germination in petri dish method were used to test the inhibitory effect of KA08 on mycelia growth and spore germination. Electrical conductivity method was used to test the permeability of pathogen plasma membrane, and the ultraviolet absorption method was employed to detect the content of ergosterol, MDA and soluble protein.【Result】KA08 had a remarkable inhibition effect on mycelia growth and spore germination. It caused shorter and twisted nodes, abnormal tubes with tips expanded or deformed, or saclike expansions. Twenty-four hours after treatment, the EC50 of KA08 against conidial germination of A. alternata was 177.53 µg•mL-1. Studies on the action mechanism of KA08 found out that the electrical conductivity of the cultural filtrate increased obviously after the pathogen was treated with KA08, indicating the leakage of protoplasm from the mycelia. The ergosterol content in the cell membrane of mycelia decreased, remarkably. The content of MDA in the mycelia and the cultural filtrate increased notably, and the protein content in the mycelia reduced greatly.【Conclusion】KA08, the active metabolite of strain 182-2, has obvious inhibitory activities against A. alternata. Its bioactivity lies in its doing damage to the pathogen cell membrane and increasing the permeability of plasma membrane through inhibiting the synthesis of ergosterol in the cell membrane and causing lipid peroxidation of the cell membrane, which resulted in the poor and abnormal mycelia growth. These results suggest that cell membrane is one of the main action sites of KA08.

Key words: Streptomyces cacaoi strain 182-2 , active metabolite , Alternaria alternata , action mechanism

GAO Fen-12, WU Yuan-Hua-2, WANG Meng-Liang-1. Action of the Metabolite Produced by Streptomyces cacaoi Strain 182-2 on Alternaria alternata[J].Scientia Agricultura Sinica, 2013, 46(23): 4933-4940.

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Action of the Metabolite Produced by Streptomyces cacaoi Strain 182-2 on Alternaria alternata

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