Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (20): 4388-4396.doi: 10.3864/j.issn.0578-1752.2013.20.023

• RESEARCH NOTES • Previous Articles    

Expression Features of BnMAPK1 in Wound and Pathogenetic Fungi Stress

 LU  Jun-Xing, LU  Qi-Feng, ZHANG  Kai, CHAI  You-Rong, LI  Jia-Na, QIAN  Wei, LU Jun , LU  Kun, LIANG  Ying   

  1. College of Agronomy and Biotechnology, Southwest University/Chongqing Rapeseed Engineering and Technology Research Center/Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715
  • Received:2013-04-23 Online:2013-10-15 Published:2013-07-19

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Abstract: 【Objective】The objective of this study is to analyze tissue specific expression of BnMAPK1 genes, and explore their characteristics and signaling pathway in wound and pathogenetic fungi stress.【Method】On the basis of silico cloning, the promoters of BnMAPK1 were cloned. BnMAPK1 mRNA expression characteristics after induced were analyzed by Quantitative real-time PCR (qRT-PCR). 【Result】The cloned promoter sequences of BnMAPK1 contain several hormone and stress responsive elements. BnMAPK1 expression could be induced by phytohormones methyl jasmonate (MeJA), salicylic acid (SA), abscisic acid (ABA), hydrogen peroxide (H2O2), wound, and Sclerotinia sclerotiorum. Further experiments showed that PDF1.2 gene was activated but PR-1 was suppressed after wound and S.sclerotinrum treatments, indicate that oilseed rape most likely through jasmonic acid (JA) signaling in response to wound and pathogenetic fungi stress.【Conclusion】BnMAPK1 expression could be induced by several phytohormones and its promoter contains several hormone and stress responsive elements. BnMAPK1 may play an important role in resistance to a variety of stress in plants and oilseed rape might be mediated by JA signaling pathway in response to wound and pathogenetic fungi stress.

Key words: Brassica napus , BnMAPK1 gene , wound , pathogenetic fungi , signaling pathway

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