Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (13): 2833-2844.doi: 10.3864/j.issn.0578-1752.2013.13.020

• RESEARCH NOTES • Previous Articles    

Acaricidal Activity of Bisdemethoxycurcumin and N-methylpyrazolebisdemethoxycurcumin Against Tetranychus cinnabarinus (Boisduval) and Their Effects on Enzymes Activity in the Mite

 LUO  Jin-Xiang, DING  Wei, ZHANG  Yong-Qiang, YANG  Zhen-Guo, LI  Yang, DING  Li-Juan   

  1. College of Plant Protection, Southwest University, Chongqing 400715
  • Received:2012-10-08 Online:2013-07-01 Published:2013-01-18

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Abstract: 【Objective】The objectives of this study are to investigate the acaricidal activity of bisdemethoxycurcumin (BDMC) and N-methylpyrazolebisdemethoxycurcumin (N-MPBDMC) against Tetranychus cinnabarinus (Boisduval) and their effects on enzymes activity in the mite and to provide a basis for the further elucidation acaricidal mechanism of these two compounds.【Method】The acaricidal activity of BDMC and N-MPBDMC against female adults of T. cinnabarinus was tested using the slide dip method and the poisoning symptoms of BDMC and N-MPBDMC exposed mites were carefully observed. The activities of acetylcholinesterase (AchE), Ca2+-ATP enzyme (Ca2+-ATPase), monoamine oxidase enzyme (MAO), glutathione S-transferases (GSTs) and carboxylesterase (CarE) of T. cinnabarinus treated with these two compounds were also determined.【Result】The results of acaricidal bioassay indicated that N-MPBDMC exhibited more pronounced acaricidal activity than BDMC against female adults of T. cinnabarinus. After 72 h of T. cinnabrinus treatment, the LC50 of N-MPBDMC was 21.77 mg•L-1, the acaricidal potency was about 10.03-fold higher than BDMC and comparable with that of 95% pyridaben TC. Some similar neurotoxic symptoms were observed in BDMC and N-MPBDMC-exposed mites. After T. cinnabrinus treatment with BDMC and N-MPBDMC, respectively, the activities of GSTs and CarE were strongly rising whereas the activities of AchE, Ca2+-ATPase and MAO were declining, the average activities of AchE decreased by 16.77% and 36.57%, that of Ca2+-ATPase decreased by 17.55% and 17.45%, that of MAO decreased by 8.04% and 14.19%, while that of GSTs increased by 17.13% and 18.93%, and that of CarE increased by 26.78% and 25.98%, respectively.【Conclusion】The mortality of mites that caused by BDMC and N-MPBDMC related to the decline of neural system related enzyme activities, and the specific action target remains to be further confirmed.

Key words: bisdemethoxycurcumin , N-methylpyrazolebisdemethoxycurcumin , Tetranychus cinnabarinus , enzymes activity

[1]吾玛尔•阿布力孜, 孜比妮沙•吾布力, 阿布都拉•阿巴斯. 我国螨类研究的最新进展. 生物学通报, 2009, 44(4): 12-15.

Abliz O, Goby Z, Abbas A. Recent progress of study on mites of our country. Bulletin of Biology, 2009, 44(4): 12-15. (in Chinese)

[2]Ansaloni T, Pascual-Ruiz S, Hurtado M A, Jacas J A. Can summer and fall vegetative growth regulate the incidence of Tetranychus urticae Koch on clementine fruit? Crop Protection, 2008, 27(3/5): 459-464.

[3]He L, Xue C H, Wang J J, Li M, Lu W C, Zhao Z M. Resistance selection and biochemical mechanism of resistance to two acaricides in Tetranychus cinnabarinus (Boisduval). Pesticide Biochemistry and Physiology, 2009, 93(1): 47-52.

[4]刘波, 桂连友. 我国朱砂叶螨研究进展. 长江大学学报自科版: 农学卷, 2007, 4(3): 9-12.

Liu B, Gui L Y. Research development of Tetranychus cinnabarinus from our country. Journal of Yangtze University: Natural Science Edition: Agricultural Science Volume, 2007, 4(3): 9-12. (in Chinese)

[5]梁为, 师光禄, 成军,卜春亚, 靳永胜, 王有年, 王战全. 瑞香狼毒中3种杀螨活性成分的致死规律. 中国农学通报, 2011, 27(9): 372-376.

Liang W, Shi G L, Cheng J, Bu C Y, Jin Y S, Wang Y N, Wang Z Q. Study on mortality law of three kinds of acaricidal active ingredients extracted from Stellera chamaejasme L. Chinese Agricultural Science Bulletin, 2011, 27(9): 372-376. (in Chinese)

[6]梁为, 白雪娜, 马兰青, 师光禄, 王有年. 东莨菪素对朱砂叶螨的毒力及杀螨机理初探. 广东农业科学, 2011(8): 68-71.

Liang W, Bai X N, Ma L Q, Shi G L, Wang Y N. Preliminary study on scopoletin toxicity to Tetranychus cinnabarinus and its acaricidal mechanism. Guangdong Agricultural Science, 2011(8): 68-71. (in Chinese)

[7]段丹丹, 王有年, 成军, 仝宝生, 师光禄. 旋覆花提取物对朱砂叶螨的生物活性及酶活性的影响. 生态学报, 2011, 31(8): 2203-2208.

Duan D D, Wang Y N, Cheng J, Tong B S, Shi G L. The toxicity of lupeol of Inula britanica on Tetranychus cinnabarinus and its effects on mite enzyme activity. Acta Ecologica Sinica, 2011, 31(8): 2203-2208. (in Chinese)

[8]杜娟, 赵磊, 师光禄, 王有年. 棕榈油酸甲酯对朱砂叶螨生物活性的影响. 北京农学院学报, 2010, 25(2): 25-28.

Du J, Zhao L, Shi G L, Wang Y N. Acarocidal activity of methyl cis-9-hexadecenoate against Tetranychus cinnabarinus. Journal of Beijing University of Agriculture, 2010, 25(2): 25-28. (in Chinese)

[9]Ahsan H, Parveen N, Khan N U, Hadi S M. Pro-oxidant, anti-oxidant and cleavage activities on DNA of curcumin and its derivatives demethoxycurcumin and bisdemethoxycurcumin. Chemico-Biological Interactions, 1999, 121(2): 161-175.

[10]Jayaprakasha G K, Rao L J, Sakariah K K. Antioxidant activities of curcumin, demethoxycurcumin and bisdemethoxycurcumin. Food Chemistry, 2006, 98(4): 720-724.

[11]Kim A N, Jeon W K, Lee J J, Kim B C. Up-regulation of heme oxygenase-1 expression through CaMKII-ERK1/2-Nrf2 signaling mediates the anti-inflammatory effect of bisdemethoxycurcumin in LPS-stimulated macrophages. Free Radical Biology and Medicine, 2010, 49(3): 323-331.

[12]Yodkeeree S, Chaiwangyen W, Garbisa S, Limtrakul P. Curcumin, demethoxycurcumin and bisdemethoxycurcumin differentially inhibit cancer cell invasion through the down-regulation of MMPs and uPA. Journal of Nutritional Biochemistry, 2009, 20(2): 87-95.

[13]Jang E M, Choi M S, Jung U J, Kim M J, Kim H J, Jeon S M, Shin S K, Seong C N, Lee M K. Beneficial effects of curcumin on hyperlipidemia and insulin resistance in high-fat-fed hamsters. Metabolism Clinical and Experimental, 2008, 57(11): 1576-1583.

[14]Matsunaga T, Endo S, Soda M, Zhao H T, Ei-Kabbani O, Tajima K, Hara A. Potent and selective inhibition of the tumor marker AKR1B10 by bisdemethoxycurcumin: Probing the active site of the enzyme with molecular modeling and site-directed mutagenesis. Biochemical and Biophysical Research Communications, 2009, 389(1): 128-132.

[15]张永强, 丁伟, 赵志模, 王进军, 廖涵杰. 姜黄对朱砂叶螨的生物活性. 植物保护学报, 2004, 31(4): 390-394.

Zhang Y Q, Ding W, Zhao Z M, Wang J J, Liao H J. Research on acarcidal bioactivities of turmeric, Curcuma longa. Acta Phytophylacica Sinica, 2004, 31(4): 390-394. (in Chinese)

[16]张永强, 丁伟, 赵志模. 姜黄素类化合物对朱砂叶螨的生物活性. 昆虫学报, 2007, 50(12): 1304-1308.

Zhang Y Q, Ding W, Zhao Z M. Biological activities of curcuminoids against Tetranychus cinnabarinus Boisduval (Acari: Tetranychidae). Acta Entomologica Sinica, 2007, 50(12): 1304-1308. (in Chinese)

[17]Busvine J R. Recommended Methods for Measurement of Pest Resistance to Pesticides. Rome, Italy: FAO Plant Production and Protection Paper, 1980: 49-54.

[18]深见顺一. 农药实验法-杀虫剂篇. 北京: 农业出版社, 1981: 101-102.

Junichi F. Pesticide Experiment-Insecticide Article. Beijing: Agriculture Press, 1981:101-102. (in Chinese)

[19]Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 1976, 72: 248-254.

[20]Ellman G L, Courtney K D, Andres Jr. V, Featherstone R M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 1961, 7(2): 88-90.

[21]Van Asperen K. A study of housefly esterase by means of a sensitive colorimetric method. Journal of Insect Physiology, 1962, 8(3): 401-416.

[22]Dekeyser M A, Downer R. G. Biochemical and physiological targets for miticides. Pesticide Science, 1994, 40(2): 85-101.

[23]王镜岩, 朱圣庚, 徐长法. 生物化学. 北京: 高等教育出版社, 1999: 39-44.

Wang J Y, Zhu S G, Xu C F. Biochemistry. Beijing: Higher Education Press, 1999: 39-44. (in Chinese)

[24]刘素媛, 孙黎光, 邢伟, 万伯建. 慢性染铅对大鼠海马区神经细胞Ca2+浓度及Ca2+-ATP 酶活性的影响. 卫生毒理学杂志, 1999, 13(l): 16-17.

Liu S Y, Sun L G, Xing W, Wan B J. The effect of chronic lead exposure on hippocampal intraneuronal [Ca2+] and activity of Ca2+-ATPase in rats. Journal of Health Toxicology, 1999, 13(1): 16-17. (in Chinese)

[25]王有年, 李青, 李照会, 师光禄. 萹蓄提取物对朱砂叶螨的触杀活性及相关酶活性的影响. 林业科学, 2010, 46(10): 103-107.

Wang Y N, Li Q, Li Z H, Shi G L. Acaricidal activities of Polygonum aviculare extracts against Tetranychus cinnabarinus and their effects on enzyme activities in the mite. Scientia Silvae Sinicae, 2010, 46(10): 103-107. (in Chinese)

[26]王燕, 吴振宇, 杜艳丽, 师光禄, 陈梅, 王海香, 王有年. 牵牛子种子提取物对朱砂叶螨触杀活性的测定. 中国农业科学, 2009, 42(8): 2973-2800.

Wang Y, Wu Z Y, Du Y L, Shi G L, Chen M, Wang H X, Wang Y N. Acaricidal activity of an extract of Pharbitis purpurea seeds against Tetranychus cinnabarinus. Scientia Agricultura Sinica, 2009, 42(8): 2973-2800. (in Chinese)

[27]Kohen R, Nyska A. Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicologic Pathology, 2002, 30(6): 620-650.

[28]滕霞, 孙曼雾. 羧酸酯酶研究进展. 生命科学, 2003, 15(1): 31-35.

Teng X, Sun M W. Advances on carboxylesterase research. Chinese Bulletin of Life Sciences, 2003, 15(1): 31-35. (in Chinese)

[29]刘素琪, 顾雅静, 王海香, 董海龙, 师光禄, 曹挥. 大戟狼毒对朱砂叶螨的毒性作用. 林业科学, 2010, 46(10): 91-94.

Liu S Q, Gu Y J, Wang H X, Dong H L, Shi G L, Cao H. Acaricidal actions of Euphorbia fischeriana against Tetranychus cinnabarinus. Scientia Silvae Sinicae, 2010, 46(10): 91-94. (in Chinese)

[30]曹挥, 王有年, 张铁强, 赵莉蔺, 刘素琪, 师光禄. 河朔荛花对山楂叶螨作用机制的初步研究. 林业科学, 2007, 43(8): 65-70.

Cao H, Wang Y N, Zhang T Q, Zhao L L, Liu S Q, Shi G L. Acaricidal actions of Wikstroemiae chamedaphne against Tetranychus viennensis. Scientia Silvae Sinicae, 2007, 43(8): 65-70. (in Chinese)

[31]师光禄, 王有年, 王鸿雷, 赵莉蔺, 刘素琪, 曹挥, 于同泉, 路苹. 万寿菊根提取物对山楂叶螨谷胱甘肽S-转移酶和蛋白酶及蛋白质含量的影响. 应用生态学报, 2007, 18(2): 400-404.

Shi G L, Wang Y N, Wang H L, Zhao L L, Liu S Q, Cao H, Yu T Q, Lu P. Effects of Tagetes erecta extracts on glutathione S-transferase and protease activities and protein content in Tetranychus viennensis. Chinese Journal of Applied Ecology, 2007, 18(2): 400-404. (in Chinese)
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