Abstract: 【Objective】This aim of this study was to determine the best extraction technique, the suitable solvent and the optimal plant parts and acaricidal activities temporal and spatial dynamic of Artemisia annua L.【Method】This paper dealt with the acarcidal bioactivities against Tetranychus cinnabarinus of total 135 extracts of petroleum ether 30-60℃, petroleum ether 60-90℃, ethanol, acetone and water parallel and sequenced extracts from the leaves, stems and roots of different period traditional Chinese medicine Artemisia annua L. in April, May, June, July and September 2006. All extracts were diluted to 5mg/ml, and examined their bioactivities against T. cinnabarinus in laboratory. 【Results】The results showed that at the concentration of 5mg/ml, the acaricidal bioactivities elevated as the development of A. annua plants, the general tendency exhibited July> June> May> April, but the September decreased comparing with the July. However, the most effective extracts in five months were all acetone parallel extract of A. annua leaf, and the corrected mortalities after 48h treatment were ranged from 74% to 100%. The median lethal concentrations (LC50) of acetone parallel extracts of A. annua leaves in September, July, June, May and April were 0.5986, 0.4341, 0.8376, 0.9443 and 1.3817mg/ml, respectively treated after 48h against T. cinnabarinus. The 13 groups were separated from acetone extracts of A. annua leaves in July by column chromatography, all of which the 11th and 12th groups exhibited strong activities. The median lethal concentrations of the 11th and 12th groups against T. cinnabarinus were 0.3683and 0.1586mg/ml, respectively. 【Conclusion】The acetone parallel extract of Artemisia annua leaf in July was most toxicity to T. cinnabarinus Bois. and the corrected mortality was 100% after exposure 48h. The No. 11 and No. 12 groups of acetone parallel extract was the most active components, could be acted as the emphases in further study.

Key words: Artemisia annua, Tetranychus cinnabarinus, Acetone parallel extract, bioactivities