Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (10): 2032-2040.doi: 10.3864/j.issn.0578-1752.2013.10.008

• PLANT PROTECTION • Previous Articles     Next Articles

Identification of Isolates of Verticillium Species from Cotton

 ZHU  He-Qin, FENG  Zi-Li, LI  Zhi-Fang, SHI  Yong-Qiang, ZHAO  Li-Hong, LI  Cai-Hong, LIU  Yi-Jie, WANG  Ling-Fei   

  1. 1.State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, Henan
    2.College of Plant Protection, Northwest A＆F University, Yangling 712100, Shaanxi
  • Received:2012-12-13 Online:2013-05-15 Published:2013-01-25

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Abstract: 【Objective】In the genus of Verticillium, there are five ‘species’ as plant pathogens which could lead to plant wilt symptom. The aim of this study was to identify the specific ‘species’ caused Verticillium wilt of cotton.【Method】A total of 310 Verticillium isolates were obtained from 84 counties, 12 provinces of China. To clarify the ‘species’ of them, both observation on biological characteristics and molecular identification were used.【Result】A total of 298 isolates were typical V. dahliae producing black microsclerotia and verticillate conidiophores identified by specific PCR amplifications. However, 12 special isolates producing non-microsclerotia, black mycelia or chlamydospore were tested by temperature sensitivity, specific PCR amplification and ITS sequencing. Except for 2 V. nigrescens isolates, the rest 10 isolates were identified as V. dahliae, no V. albo-atrum was found. 【Conclusion】 In 3 main cotton growing regions of China, the causal agent of cotton Verticillium wilt is V. dahliae. And V. nigrescens also presented in Yangzi River and Yellow River basins. In cotton Verticillium wilt happening process, the interaction between V. dahliae and V. nigrescens needs to be further studied.

Key words: Verticillium dahlia , Verticillium nigrescens , Verticillium albo-atrum , cotton Verticillium wilt

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