碳同位素在草地土壤呼吸区分中的应用

doi:10.3864/j.issn.0578-1752.2012.17.010

Abstract

Abstract: Grassland ecosystem plays an important role in regional climate changes and global carbon cycle. The partitioning of soil respiration in grassland ecosystem is the foundation of quantitative evaluation of grassland vegetation and soil carbon balance, which could help to improve understanding and prediction of the responses of grassland ecosystem to climate changes. In this paper, several methods for separating soil respiration in grassland ecosystem by the application of stable carbon isotope 13C and radioactive carbon isotope 14C were reviewed. Methods for the separation of SOM-derived respiration and root-derived respiration mainly include: 13C natural abundance method, FACE experiment, 13C pulse labeling. Also, the methods for the separation of root and rhizomicrobial respiration mainly include: isotope dilution method, model rhizodeposition method, modeling of 14CO2 efflux dynamics method, and exudates elution method. Carbon isotope methods are novel methods that involve less disturbances to soil-plant system, which ultimately enhance the accuracy of estimation. Overall, carbon isotope methods have a great potential in the study of partitioning of soil respiration in grassland ecosystem.

Key words: 13C, 14C, grassland ecosystem, soil respiration, partitioning

GENG Yuan-Bo, SHI Jing-Jing. Application of the Carbon Isotope in the Partitioning of Soil Respiration in Grassland[J].Scientia Agricultura Sinica, 2012, 45(17): 3541-3550.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2012.17.010

https://www.chinaagrisci.com/EN/Y2012/V45/I17/3541

References

[1]Högberg P, Read D J. Towards a more plant physiological perspective on soil ecology. Trends in Ecology and Evolution, 2006, 21(10): 548-554.

[2]Singh J S, Gupta S R. Plant decomposition and soil respiration in terrestrial ecosystems. The Botanical Review, 1977, 43(4): 449-528.

[3]Hanson P J, Edwards N T, Garten C T, Andrews J A. Separating root and soil microbial contributions to soil respiration: A review of methods and observations. Biogeochemistry, 2000, 48: 115-146.

[4]Schlesinger W H, Andrews J A. Soil respiration and the global carbon cycle. Biogeochemistry, 2000, 48(1): 7-20.

[5]Rustad L E, Huntington T G, Boone R D. Controls on soil respiration: implications for climate change. Biogeochemistry, 2000, 48(1): 1-6.

[6]Jia B R, Zhou G S, Wang F Y, Wang Y H, Yuan W P, Zhou L. Partitioning root and microbial contributions to soil respiration in Leymus chinensis populations. Soil Biology and Biochemistry, 2006, 38(4): 653-660.

[7]Boone R D, Nadelhoffer K J, Canary J D, Kaye J P. Roots exert a strong influence on the temperature sensitivity of soil respiration. Nature, 1998, 396(6711): 570-572.

[8]Cheng W X. Measurement of rhizosphere respiration and organic matter decomposition using natural 13C. Plant and Soil, 1996, 183: 263-268.

[9]Killham K, Yeomans C. Rhizosphere carbon flow measurement and implications: from isotopes to reporter genes. Plant and Soil, 2001, 232(1): 91-96.

[10]Kuzyakov Y. Separating microbial respiration of exudates from root respiration in non-sterile soils: a comparison of four methods. Soil Biology and Biochemistry, 2002, 34(11): 1621-1631.

[11]方精云, 王娓. 作为地下过程的土壤呼吸：我们理解了多少？植物生态学报, 2007, 31(3): 345-347.

Fang J Y, Wang W. Soil respiration as a key belowground process: issues and perspectives. Journal of Plant Ecology, 2007, 31(3): 345-347. (in Chinese)

[12]金钊, 董云社, 齐玉春. 区分纯根呼吸和根际微生物呼吸的争议. 土壤, 2008, 40(4): 517-522.

Jin Z, Dong Y S, Qi Y C. On disputation about partitioning respiration of actual root and rhizomicrobes. Soils, 2008, 40(4): 517-522. (in Chinese)

[13]Kuzyakov Y. Review: Factors affecting rhizosphere priming effects. Journal of Plant Nutrition and Soil Science, 2002, 165(4): 382-396.

[14]Kuzyakov Y. Separation of root and rhizomicrobial respiration by natural 13C abundance: theoretical approach, advantages, and difficulties. Eurasian Soil Science, 2004, 37(Suppl.1): 79-84.

[15]White R P, Murray S, Rohweder M. Pilot Analysis of Global Ecosystems (PAGE): Grassland Ecosystems. Washington, DC: World Resources Institute, 2000.

[16]Anderson J M. The effects of climate change on decomposition processes in grassland and coniferous forests. Ecological Applications, 1991, 1(3): 326-347.

[17]Eswaran H, van den Berg E, Reich P. Organic carbon in soils of the world. Soil Science Society of America Journal, 1993, 57: 192-194.

[18]Conant R T, Paustian K, Elliott E T. Grassland management and conversion into grassland: effects on soil carbon. Ecological Applications, 2001, 11(2): 343-355.

[19]Hunt J E, Kelliher F M, McSeveny T M, Ross D J, Whitehead D. Longterm carbon exchange in a sparse, seasonally dry tussock grassland. Global Change Biology, 2004, 10(10): 1785-1800.

[20]Wohlfahrt G, Anfang C, Bahn M, Haslwanter A, Newesely C, Schmitt M, Drösler M, Pfadenhauer J, Cernusca A. Quantifying nighttime ecosystem respiration of a meadow using eddy covariance, chambers and modelling. Agricultural and Forest Meteorology, 2005, 128(3/4): 141-162.

[21]Luo Y Q, Zhou X H. Soil Respiration and the Environment. San Diego: Academic Press, 2006.

[22]于贵瑞, 孙晓敏. 陆地生态系统通量观测的原理与方法. 北京: 高等教育出版社, 2006.

Yu G R, Sun X M. Principles of Flux Measurement in Terrestrial Ecosystems. Beijing: Higher Education Press, 2006. (in Chinese)

[23]Gloser J, Tesarova M. Litter, soil, and root respiration measurement. An improved compartmental analysis method. Pedobiologia, 1978, 18(1): 76-81.

[24]刘立新, 董云社, 齐玉春, 周凌晞. 应用根去除法对内蒙古温带半干旱草原根系呼吸与土壤总呼吸的区分研究. 环境科学, 2007, 28(4): 689-694.

Liu L X, Dong Y S, Qi Y C, Zhou L X. Study of distinguish root respiration from total soil respiration by root exclusion method in the temperate semi-arid grassland in Inner Mongolia, China. Environmental Science, 2007, 28(4): 689-694. (in Chinese)

[25]Bao F, Zhou G S, Wang F Y, Sui X H. Partitioning soil respiration in a temperate desert steppe in Inner Mongolia using exponential regression method. Soil Biology and Biochemistry, 2010, 42(12): 2339-2341.

[26]刘立新, 董云社, 齐玉春. 锡林河流域生长季节不同草地类型根系呼吸特征研究. 环境科学, 2006, 12: 2376-2381.

Liu L X, Dong Y S, Qi Y C. Study on the free-grazed steppes root respiration of growing season in the Xilin River Basin, China. Environmental Science, 2006, 12: 2376-2381. (in Chinese)

[27]Craine J M, Wedin D A. Determinants of growing season soil CO2 flux in a Minnesota grassland. Biogeochemistry, 2002, 59(3): 303-313.

[28]Millard P, Midwood A J, Hunt J E, Whitehead D, Boutton T W. Partitioning soil surface CO2 efflux into autotrophic and heterotrophic components, using natural gradients in soil δ13C in an undisturbed savannah soil. Soil Biology and Biochemistry, 2008, 40(7): 1575-1582.

[29]Wu Y B, Tan H C, Deng Y C, Wu J, Xu X L, Wang Y F, Tang Y H, Higashi T, Cui X Y. Partitioning pattern of carbon flux in a Kobresia grassland on the Qinghai-Tibetan Plateau revealed by field 13C pulse-labeling. Global Change Biology, 2010, 16(8): 2322-2333.

[30]Del Galdo I, Six J, Peressotti A, Cotrufo M F. Assessing the impact of land-use change on soil C sequestration in agricultural soils by means of organic matter fractionation and stable C isotopes. Global Change Biology, 2003, 9(8): 1204-1213.

[31]Ehleringer J R, Buchmann N, Flanagan L B. Carbon isotope ratios in belowground carbon cycle processes. Ecological Applications, 2000, 10(2): 412-422.

[32]Andrews J A, Harrison K G, Matamala R, Schlesinger W H. Separation of root respiration from total soil respiration using carbon-13 labeling during free-air carbon dioxide enrichment (FACE). Soil Science Society of America Journal, 1999, 63: 1429-1435.

[33]Bertolini T, Inglima I, Rubino M, Marzaioli F, Lubritto C, Subke J A, Peressotti A, Cotrufo F. Sampling soil-derived CO2 for analysis of isotopic composition: a comparison of different techniques. Isotopes in Environmental and Health Studies, 2006, 42(1): 57-65.

[34]Kuzyakov Y. Sources of CO2 efflux from soil and review of partitioning methods. Soil Biology and Biochemistry, 2006, 38: 425-448.

[35]杨杰东, 徐士进. 同位素与全球环境变化. 北京: 地质出版社, 2007.

Yang J D, Xu S J. Isotopes and Global Environment Changes. Beijing: Geology Press, 2007. (in Chinese)

[36]O'Leary M H. Carbon isotopes in photosynthesis. BioScience, 1988, 38(5): 328-336.

[37]Coleman D C, Fry B. Carbon Isotope Techniques. San Diego: Academic Press,1991.

[38]Bender M M. Mass spectrometric studies of carbon 13 variations in corn and other grasses. Radiocarbon, 1968, 10(2): 468-472.

[39]Bender M M. Variations in the 13C/12C ratios of plants in relation to the pathway of photosynthetic carbon dioxide fixation. Phytochemistry, 1971, 10(6): 1239-1244.

[40]Smith B N, Epstein S. Two categories of 13C/12C ratios for higher plants. Plant Physiology, 1971, 47(3): 380-384.

[41]Balesdent J, Mariotti A, Guillet B. Natural 13C abundance as a tracer for studies of soil organic matter dynamics. Soil Biology and Biochemistry, 1987, 19(1): 25-30.

[42]Cerri C, Feller C, Balesdent J, Victoria R, Plenecassagne A. Application du traçage isotopique naturel en 13C, à l'étude de la dynamique de la matière organique dans les sols. Comptes-rendus des séances de l'Académie des sciences. Série 2, Mécanique-physique, chimie, sciences de l'univers, sciences de la terre, 1985, 300(9): 423-428.

[43]Beerling D J, Osborne C P. The origin of the savanna biome. Global Change Biology, 2006, 12(11): 2023-2031.

[44]Rochette P, Flanagan L B, Gregorich E G. Separating soil respiration into plant and soil components using analyses of the natural abundance of carbon-13. Soil Science Society of America Journal, 1999, 63(5): 1207-1213.

[45]Bathellier C, Badeck F W, Couzi P, Harscoët S, Mauve C, Ghashghaie J. Divergence in δ13C of dark respired CO2 and bulk organic matter occurs during the transition between heterotrophy and autotrophy in Phaseolus vulgaris plants. New Phytologist, 2008, 177(2): 406-418.

[46]Werth M, Kuzyakov Y. Below-ground partitioning (14C) and isotopic fractionation (δ13C) of carbon recently assimilated by maize. Isotopes in Environmental and Health Studies, 2005, 41(3): 237-248.

[47]Werth M, Kuzyakov Y. 13C-fractionation at the root-microorganisms-soil interface: A review and outlook for partitioning studies. Soil Biology and Biochemistry, 2010, 42(9): 1372-1384.

[48]Fritz P, Mozeto A A, Reardon E J. Practical considerations on carbon isotope studies on soil carbon dioxide. Chemical Geology: Isotope Geoscience Section, 1985, 58(1/2): 89-95.

[49]Bird M I, Pousai P. Variations of δ13C in the surface soil organic carbon pool. Global Biogeochemical Cycles, 1997, 11(3): 313-322.

[50]刘钢, 韩勇, 朱建国, 冈田益已, 中村浩史, 吉本真由美. 稻麦轮作 FACE 系统平台 I. 系统结构与控制. 应用生态学报, 2002, 13(10): 1253-1258.

Liu G, Han Y, Zhu J G, Okada M, Nakamura H, Yoshimoto M. Rice-wheat rotational FACE platform I. System structure and control. Chinese Journal of Applied Ecology, 2002, 13(10): 1253-1258. (in Chinese)

[51]崔向慧, 李昊, 卢琦, 林光辉. 全球FACE实验的进展与展望. 世界林业研究, 2007, 20(5): 1-6.

Cui X H, Li H, Lu Q, Lin G H. Global progress and prospects on FACE (Free-Air CO2 Enrichment) experiment. World Forestry Research, 2007, 20(5): 1-6. (in Chinese)

[52]罗光强, 耿元波, 袁国富. 碳同位素在草地生态系统碳循环中的应用与展望. 地理科学进展, 2009, 28(3): 441-448.

Luo G Q, Geng Y B, Yuan G F. Application and prospect of carbon isotope in the study of carbon cycle in grassland ecosystem. Progress in Geography, 2009, 28(3): 441-448. (in Chinese)

[53]Leavitt S W, Pendall E, Paul E A, Brooks T, Kimball B A, Pinter P J, Jr, Johnson H B, Matthias A, Wall G W, LaMorte R L. Stable-carbon isotopes and soil organic carbon in wheat under CO2 enrichment. New Phytologist, 2001, 150(2): 305-314.

[54]Schimel D S, Braswell B H, Holland E A, McKeown R, Ojima D S, Painter T H, Parton W J, Townsend A R. Climatic, edaphic, and biotic controls over storage and turnover of carbon in soils. Global Biogeochemical Cycles, 1994, 8(3): 279-293.

[55]Torn M S, Trumbore S E, Chadwick O A, Vitousek P M, Hendricks D M. Mineral control of soil organic carbon storage and turnover. Nature, 1997, 389(6647): 170-173.

[56]Pendall E, Del Grosso S, King J Y, LeCain D R, Milchunas D G, Morgan J A, Mosier A R, Ojima D S, Parton W A, Tans P P. Elevated atmospheric CO2 effects and soil water feedbacks on soil respiration components in a Colorado grassland. Global Biogeochemical Cycles, 2003, 17(2): 15-16.

[57]Van Kessel C, Nitschelm J, Horwath W R, Harris D, Walley F, Lüscher A, Hartwig U. Carbon-13 input and turn-over in a pasture soil exposed to long-term elevated atmospheric CO2. Global Change Biology, 2000, 6(1): 123-135.

[58]Hungate B A, Chapin F S, III, Zhong H, Holland E A, Field C B. Stimulation of grassland nitrogen cycling under carbon dioxide enrichment. Oecologia, 1996, 109(1): 149-153.

[59]Merckx R, Den Hartog A, Van Veen J A. Turnover of root-derived material and related microbial biomass formation in soils of different texture. Soil Biology and Biochemistry, 1985, 17(4): 565-569.

[60]Stewart D P C, Metherell A K. Carbon (13C) uptake and allocation in pasture plants following field pulse-labelling. Plant and Soil, 1999, 210(1): 61-73.

[61]Wang Z P, Li L H, Han X G, Li Z Q, Chen Q S. Dynamics and allocation of recently photo-assimilated carbon in an Inner Mongolia temperate steppe. Environmental and Experimental Botany, 2007, 59(1): 1-10.

[62]Hafner S, Unteregelsbacher S, Seeber E, Lena B, Xu X, Li X, Guggenberger G, Miehe G, Kuzyakov Y. Effect of grazing on carbon stocks and assimilate partitioning in a Tibetan montane pasture revealed by 13CO2 pulse labeling. Global Change Biology, 2011, 18(2): 528-538.

[63]何敏毅, 孟凡乔, 史雅娟, 吴文良. 用13C脉冲标记法研究玉米光合碳分配及其向地下的输入. 环境科学, 2008, 29(2): 446-453.

He M Y, Meng F Q, Shi Y J, Wu W L. Estimating photosynthesized carbon distribution and inputs into belowground in a maize soil following 13C pulse labeling. Environmental Science, 2008, 29(2): 446-453. (in Chinese)

[64]Meharg A A. A critical review of labelling techniques used to quantify rhizosphere carbon-flow. Plant and Soil, 1994, 166(1): 55-62.

[65]Cheng W X, Coleman D C, Carroll C R, Hoffman C A. In situ measurement of root respiration and soluble C concentrations in the rhizosphere. Soil Biology and Biochemistry, 1993, 25(9): 1189-1196.

[66]Kuzyakov Y, Ehrensberger H, Stahr K. Carbon partitioning and below-ground translocation by Lolium perenne. Soil Biology and Biochemistry, 2001, 33(1): 61-74.

[67]Kuzyakov Y, Cheng W. Photosynthesis controls of rhizosphere respiration and organic matter decomposition. Soil Biology and Biochemistry, 2001, 33(14): 1915-1925.

[68]Swinnen J. Evaluation of the use of a model rhizodeposition technique to separate root and microbial respiration in soil. Plant and Soil, 1994, 165(1): 89-101.

[69]Merbach W, Mirus E, Knof G, Remus R, Ruppel S, Russow R, Gransee A, Schulze J. Release of carbon and nitrogen compounds by plant roots and their possible ecological importance. Journal of Plant Nutrition and Soil Science, 1999, 162(4): 373-383.

[70]Rovira A D. Zones of exudation along plant roots and spatial distribution of micro-organisms in the rhizosphere. Pesticide Science, 1973, 4(3): 361-366.

[71]Kuzyakov Y, Domanski G. Model for rhizodeposition and CO2 efflux from planted soil and its validation by 14 C pulse labelling of ryegrass. Plant and Soil, 2002, 239(1): 87-102.

[72]Kuzyakov Y, Kretzschmar A, Stahr K. Contribution of Lolium perenne rhizodeposition to carbon turnover of pasture soil. Plant and Soil, 1999, 213(1): 127-136.

[73]Warembourg F R, Billes G. Estimating carbon transfers in the plant rhizosphere//Harley J L, Russell R S. The Soil-root Interface. London, New York, San Francisco: New Phytologist Academic Press, 1979: 183-196.

[74]Kuzyakov Y, Larionova A A. Root and rhizomicrobial respiration: A review of approaches to estimate respiration by autotrophic and heterotrophic organisms in soil. Journal of Plant Nutrition and Soil Science, 2005, 168(4): 503-520.

[75]Sapronov D V, Kuzyakov Y V. Separation of root and microbial respiration: Comparison of three methods. Eurasian Soil Science, 2007, 40(7): 775-784.

[76]Hodge A, Grayston S J, Ord B G. A novel method for characterisation and quantification of plant root exudates. Plant and Soil, 1996, 184(1): 97-104.

[77]Kuzyakov Y, Siniakina S V. A novel method for separating root-derived organic compounds from root respiration in non-sterilized soils. Journal of Plant Nutrition and Soil Science, 2001, 164(5): 511-517.

[78]Paterson E, Sim A. Rhizodeposition and C-partitioning of Lolium perenne in axenic culture affected by nitrogen supply and defoliation. Plant and Soil, 1999, 216(1): 155-164.

[79]Jones D L, Darrah P R. Re-sorption of organic compounds by roots of Zea mays L. and its consequences in the rhizosphere. Plant and Soil, 1993, 153(1): 47-59.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Application of the Carbon Isotope in the Partitioning of Soil Respiration in Grassland

PDF

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	REN JunBo,YANG XueLi,CHEN Ping,DU Qing,PENG XiHong,ZHENG BenChuan,YONG TaiWen,YANG WenYu. Effects of Interspecific Distances on Soil Physicochemical Properties and Root Spatial Distribution of Maize-Soybean Relay Strip Intercropping System [J]. Scientia Agricultura Sinica, 2022, 55(10): 1903-1916.
[2]	ZHOU YongJie,XIE JunHong,LI LingLing,WANG LinLin,LUO ZhuZhu,WANG JinBin. Effects of Long-Term Reduce/Zero Tillage and Nitrogen Fertilizer Reducing on Maize Yield and Soil Carbon Emission Under Fully Plastic Mulched Ridge-Furrow Planting System [J]. Scientia Agricultura Sinica, 2021, 54(23): 5054-5067.
[3]	CAO BinBin,ZHU YiHui,JIANG YuHan,SHI JiangLan,TIAN XiaoHong. Effects of Lime and Straw Addition on SOC Sequestration in Tier Soil [J]. Scientia Agricultura Sinica, 2020, 53(20): 4215-4225.
[4]	FAN KaiKai,TONG XuZe,YAN YuChun,XIN XiaoPing,WANG Xu. Effect of Fairy Rings on Soil Respiration in Hulunber Meadow Steppe [J]. Scientia Agricultura Sinica, 2020, 53(13): 2595-2603.
[5]	YU AiZhong, CHAI Qiang, YIN Wen, HU FaLong, FAN ZhiLong, ZHAO Cai. Responses of Soil Carbon Emission and Carbon Balance of Maize Field to Plastic Film Mulching Pattern and Row Space [J]. Scientia Agricultura Sinica, 2018, 51(19): 3726-3735.
[6]	DING XinYu, WANG ZiKui, YANG Xuan, DU ShanShan, SHEN YuYing. Response of Dry Land Soil Respiration to Conservation Tillage Practices During Drying-Wetting Cycles [J]. Scientia Agricultura Sinica, 2017, 50(24): 4759-4768.
[7]	ZHANG YanJun. Effect of Long-Term Fertilization on Temperature Sensitivity of Soil Respiration During Fallow Season [J]. Scientia Agricultura Sinica, 2017, 50(16): 3164-3174.
[8]	YU ShuTing, ZHAO YaLi, WANG YuHong, LIU WeiLing, MENG ZhanYing, MU XinYuan, CHENG SiXian, LI ChaoHai. Improvement Effects of Rotational Tillage Patterns on Soil in the Winter Wheat-Summer Maize Double Cropping Area of Huang-Huai-Hai Region [J]. Scientia Agricultura Sinica, 2017, 50(11): 2150-2165.
[9]	WANG Wei-yu, QIAO Bo, Kashif AKHTAR, YUAN Shuai, REN Guang-xin, FENG Yong-zhong. Effects of Straw Returning to Field on Soil Respiration and Soil Water Heat in Winter Wheat - Summer Maize Rotation System Under No Tillage [J]. Scientia Agricultura Sinica, 2016, 49(11): 2136-2152.
[10]	GU Yun-Qian, LIU Xue, ZHANG Wei, QI Chun-Jie, TANG Kai-Lei, ZHAO Yang, ZHANG Yan, LI Gang, WANG Bin, ZHAO Chun-Jiang, LUO Wei-Hong. The Simulation Model of the Effects of Low Level of Radiation at Milk Filling Stage on Wheat Growth and Yield [J]. Scientia Agricultura Sinica, 2013, 46(5): 898-908.
[11]	CI 恩, ZHU Jie, PENG Juan, FU Zhuo-Wang, GAO Ming, XIE De-Ti. Active Fractions and δ13C Value of Soil Organic Carbon in Paddy Fields Under Ridge-Cultivation and No Tillage System [J]. Scientia Agricultura Sinica, 2013, 46(5): 978-986.
[12]	WANG Shi-Xiong, GUO Hua, WANG Xiao-An, FAN Wei-Yi. Dispersal Limitation Versus Environment Filtering in the Assembly of Plant Communities in the Ziwu Mountains [J]. Scientia Agricultura Sinica, 2013, 46(22): 4733-4744.
[13]	SA Ru-La-1, LI Jin-Xiang-2, HOU Xiang-Yang-1. Research on Soil Organic Carbon Storage Distribution in the Grassland Ecosystem [J]. Scientia Agricultura Sinica, 2013, 46(17): 3604-3614.
[14]	ZHANG Qian-Bing, YANG Ling, WANG Jin, LUO Hong-Hai, ZHANG Ya-Li, ZHANG Wang-Feng. Effects of Different Irrigation Methods and Fertilization Measures on Soil Respiration and Its Component Contributions in Cotton Field in Arid Region [J]. Scientia Agricultura Sinica, 2012, 45(12): 2420-2430.
[15]	HOU Xiang-Yang, XU Hai-Hong. Research on Carbon Balance of Different Grazing Systems in Stipa breviflora Desert Steppe [J]. Scientia Agricultura Sinica, 2011, 44(14): 3007-3015 .