Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (13): 2711-2717.doi: 10.3864/j.issn.0578-1752.2012.13.015

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Effect of Supplement of Soya Lecithine on Mucosal Anti-Oxidation and Heat Shock Protein 70 Content in Intrauterine Growth Retardation Piglets

 WANG  Yuan-Xiao, ZHANG  Li-Li, ZHOU  Gen-Lai, WANG  Tian   

  1. 南京农业大学动物科技学院，南京 210095
  • Received:2011-11-14 Online:2012-07-01 Published:2012-03-23

PDF

740

Cited

3

Abstract: 【Objective】The effects of supplement of soya lecithine (SL) on intestinal growth, mucosal heat shock protein 70 (HSP70) expression and oxidation stress (OS) in intrauterine growth retardation (IUGR) piglets were studied.【Method】Twelve IUGR piglets and six piglets with normal birth weight were selected. All piglets were weaned on 7th d of age, and assigned equally to three groups: six NBW and six IUGR piglets were fed with control diet (IUGR), and six IUGR piglets were fed with diet supplemented with 1.5% SL (IUGR+SL) for 7 days.【Result】IUGR decreased (P＜0.05) the absolute weigh of jejunal segment and non-mucosa, reduced (P＜0.05) the mucosal capacity of total anti-oxidation (T-AOC), as well as the activity of GR and GPx, and elevated (P＜0.05) the content of MDA. The mucosal HSP70 contents detected by ELISA and Western Blot were increased (P＜0.05) by IUGR. After SL treatment, the absolute and relative weight of jejunum, jejunal mucosa and non-mucosa increased (P＜0.05) in IUGR piglets. Supplement of SL increased (P＜0.05) the mucosal T-AOC, GPx and SOD, as well as reduced (P＜0.05) MDA and HSP70 content in terms of ELISA. Inverse correlations were found between SL supplementation in IUGR piglets and HSP70 contents (P＜0.05).【Conclusion】SL plays an important role in recovering impaired jejunal growth, increased mucosal HSP70, and OS induced by IUGR.

Key words: soya lecithine, piglets, IUGR, anti-oxidation, HSP70

[1]Rosenberg A. The IUGR newborn. Seminars in Perinatology, 2008, 32(3): 219-224.

[2]Mandruzzato G, Antsaklis A, Botet F, Chervenak F A, Figueras F, Grunebaum A, Puerto B, Skupski D, Stanojevic M. Intrauterine restriction (IUGR). Journal of Perinatal Medicine, 2008, 36(4): 277-281.

[3]Wu G, Bazer F W, Cudd T A, Meininger C J, Spencer T E. Maternal nutrition and fetal development. The Journal of Nutrition, 2004, 134(9): 2169-2172.

[4]Wang J, Chen L, Li D, Yin Y, Wang X, Li P, Dangott L J, Hu W, Wu G. Intrauterine growth restriction affects the proteomes of the small intestine, liver, and skeletal muscle in newborn pigs. The Journal of Nutrition, 2008, 138(1): 60-66.

[5]Wang T, Huo Y J, Shi F, Xu R J, Hutz R J. Effects of intrauterine growth retardation on development of the gastrointestinal tract in neonatal pigs. Biology of the Neonate, 2005, 88(1): 66-72.

[6]Zhong X, Wang T, Zhang X, Li W. Heat shock protein 70 is upregulated in the intestine of intrauterine growth retardation piglets. Cell Stress and Chaperones, 2010, 15(3): 335-342.

[7]周根来, 王  恬, 霍永久, 陈才勇, 石现瑞, 李建农. 胎儿宫内发育迟缓对新生仔猪小肠组织发育的影响. 动物营养学报, 2006, 18(1): 19-25.

Zhou G L, Wang T, Huo Y J, Chen C Y, Shi X R, Li J N. Impacts of intrauterine growth retardation on development of small intestinal tissue in newborn piglets. Chinese Journal of Animal Nutrition, 2006, 18(1): 19-25. (in Chinese)

[8]Wu G, Bazer F W, Wallace J M, Spencer T E. Board-invited review: intrauterine growth retardation: implications for the animal sciences. Journal of Animal Science, 2006, 84(9): 2316-2337.

[9]Xu R J, Mellor D J, Birtles M J, Reynolds G W, Simpson H V. Impact of intrauterine growth retardation on the gastrointestinal tract and the pancreas in newborn pigs. Journal of Pediatric Gastroenterology and Nutrition, 1994, 18(2): 231-240.

[10]赵  念, 张莉莉, 金  融, 杜  伟, 王  恬. 添加酪蛋白酶解物对宫内生长迟缓仔猪肠道生长发育的影响. 畜牧兽医学报, 2008, 39(5): 594-600.

Zhao N, Zhang L L, Jin R, Du W, Wang T. Effeccts of casein hydrolysate on the intestinal growth and development of IUGR piglets. Acta Veterinaria et Zootechnica Sinica, 2008, 39(5):594-600. (in Chinese)

[11]杜  伟, 王  恬. 乳源性生物活性肽对新生IUGR仔猪肝脏抗氧化功能的影响. 畜牧与兽医, 2007, 39(11): 12-14.

Du W, Wang T. Effects of milk-borne bioactive peptidea on antioxidation functions in the liver of neonatal IUGR pigs. Animal Husbandry and Veterinary Medicine, 2007, 39(11): 12-14. (in Chinese)

[12]沈  峰, 王  恬. 胰岛素对子宫内生长迟缓新生仔猪小肠生长发育的影响. 肠外与肠内营养, 2005, 12(1): 5-8.

Shen F, Wang T. The effect of insulin on gastrointestinal growth and development in IUGR neonatal piglets. Parenteral and Enteral Nutrition, 2005, 12(1): 5-8. (in Chinese)

[13]Ogata E S, Swanson S L, Collins J W Jr, Finley S L. Intrauterine growth retardation: Altered hepatic energy and redox states in the fetal rat. Pediatric Research, 1990, 27(1): 56-63.

[14]Hracsko Z, Orvos H, Novak Z, Pal A, Varga I S. Evaluation of oxidative stress markers in neonates with intra-uterine growth retardation. Redox Report: Communications in Free Radical Research, 2008, 13(1): 11-16.

[15]Ghyczy M, Torday C, Kaszaki J, Szabo A, Czobel M, Boros M. Oral phosphatidylcholine pretreatment decreases ischemia-reperfusion- induced methane generation and the inflammatory response in the small intestine. Shock, 2008, 30(5): 596-602.

[16]DeSchryver-Kecskemeti K, Eliakim R, Carroll S, Stenson W F, Moxley M A, Alpers D H. Intestinal surfactant-like material. A novel secretory product of the rat enterocyte. The Journal of Clinical Investigation, 1989, 84(4): 1355-1361.

[17]Haynes T E, Li P, Li X, Shimotori K, Sato H, Flynn N E, Wang J, Knabe D A, Wu G. L-glutamine or L-alanyl-L-glutamine prevents oxidant- or endotoxin-induced death of neonatal enterocytes. Amino Acids, 2009, 37(1): 131-142.

[18]Tsukimi Y, Nakai H, Itoh S, Amagase K, Okabe S. Involvement of heat shock proteins in the healing of acetic acid-induced gastric ulcers in rats. Journal of Physiology and Pharmacology: an Official Journal of the Polish Physiological Society, 2001, 52(3): 391-406.

[19]Yao K, Yin Y L, Chu W, Liu Z, Deng D, Li T, Huang R, Zhang J, Tan B, Wang W, Wu G. Dietary arginine supplementation increases mtor signaling activity in skeletal muscle of neonatal pigs. The Journal of Nutrition, 2008, 138(5): 867-872.

[20]Shulman R J. Oral insulin increases small intestinal mass and disaccharidase activity in the newborn miniature pig. Pediatric Research, 1990, 28(2): 171-175.

[21]汪纪仓, 刘学忠, 袁  燕, 裔传卉, 卞建春, 刘宗平. 氧化应激在镉致大鼠肝细胞凋亡中的作用. 中国农业科学, 2011, 44(18): 3895-3902.

Wang J C, Liu X Z, Yuan Y, Yi C H, Bian J C, Liu Z P. The role of oxidative stress on the apoptosis of rat hepatocytes induced by cadmium. Scientia Agricultura Sinica, 2011, 44(18): 3895-3902. (in Chinese)

[22]Gupta P, Narang M, Banerjee B D, Basu S. Oxidative stress in term small for gestational age neonates born to undernourished mothers: A case control study. BMC pediatrics, 2004, 4(1): 14-21.

[23]Biri A, Bozkurt N, Turp A, Kavutcu M, Himmetoglu O, Durak I. Role of oxidative stress in intrauterine growth restriction. Gynecologic and Obstetric Investigation, 2007, 64(4): 187-192.

[24]Kamath U, Rao G, Kamath S U, Rai L. Maternal and fetal indicators of oxidative stress during intrauterine growth retardation (IUGR). Indian Journal of Clinical Biochemistry, 2006, 21(1): 111-115.

[25]张志宏, 田文儒, 李辉辉, 张启耀, 高善颂. 谷氨酰胺诱导小鼠热休克蛋白70 表达的研究. 中国农业科学, 2009, 42(4): 1428-1434.

Zhang Z H, Tian W R, Li H H, Zhang Q Y, Gao S S. Inducement of heat shock protein 70 expression by glutamine in the mice. Scientia Agricultura Sinica, 2009, 42(4): 1428-1434. (in Chinese)

[26]David J C, Grongnet J F, Lalles J P. Weaning affects the expression of heat shock proteins in different regions of the gastrointestinal tract of piglets. The Journal of Nutrition, 2002, 132(9): 2551-2561.

[27]Konturek J W, Fischer H, Konturek P C, Huber V, Boknik P, Luess H, Neumann J, Brzozowski T, Schmitz W, Hahn E G, Domschke W, Konturek S J. Heat shock protein 70 (hsp70) in gastric adaptation to aspirin in helicobacter pylori infection. Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society, 2001, 52(1): 153-164.

[28]Zou J, Salminen W F, Roberts S M, Voellmy R. Correlation between glutathione oxidation and trimerization of heat shock factor 1, an early step in stress induction of the hsp response. Cell Stress and Chaperones, 1998, 3(2): 130-141.

[29]Hnat M, Meadows J, Brockman D, Pitzer B, Lyall F, Myatt L. Heat shock protein-70 and 4-hydroxy-2-nonenal adducts in human placental villous tissue of normotensive, preeclamptic and intrauterine growth restricted pregnancies. American Journal of Obstetrics and Gynecology, 2005, 193(3): 836-840.

[30]Stremmel W, Hanemann A, Braun A, Stoffels S, Karner M, Fazeli S, Ehehalt R. Delayed release phosphatidylcholine as new therapeutic drug for ulcerative colitis-a review of three clinical trials. Expert Opinion on Investigational Drugs, 2010, 19(12): 1623-1630.

[31]Holtzapple P G, Starr C M, Morck T. Phosphatidylcholine synthesis in the developing small intestine. The Biochemical Journal, 1980, 186(2): 399-403.

[32]Venneman N G, Petruzzelli M, van Dijk J E, Verheem A, Akkermans L M A, Kroese A B A, van Erpecum K J. Indomethacin disrupts the protective effect of phosphatidylcholine against bile salt-induced ileal mucosa injury. European Journal of Clinical Investigation, 2006, 36(2): 105-112.
[1] YU ZhengWang,ZHOU ZhongXin. Functions of Antibacterial and Inducing Defense Peptide Expression of Medium-Chain Fatty Acid and Its Application in Piglet Feeds [J]. Scientia Agricultura Sinica, 2021, 54(13): 2895-2905.
[2] LIN XiaJing,CHEN Fang,JIANG ShouQun,JIANG ZongYong. Effects of Soybean Isoflavones on Growth Performance, Antioxidant Performance and Intestinal Morphology of Early-Weaned Piglets [J]. Scientia Agricultura Sinica, 2020, 53(10): 2101-2111.
[3] ZHOU Min, ZHOU XueMei, YANG LiJie, HUANG LiBo, FENG Lei, SHAO MingHui, YANG Chen, YANG WeiRen, YANG ZaiBin, JIANG ShuZhen. Effects of Zearalenone on Expression of Heat Shock Protein 70 and Morphology of Uterus Tissues of Post-Weaning Piglets [J]. Scientia Agricultura Sinica, 2018, 51(4): 778-788.
[4] LI YaBo, LI Ting, HAN YingYan, FAN ShuangXi. Cloning and Function Analysis of Heat-Shock-Protein LsHsp70-2711 Gene Under High Temperature Stress in Leaf Lettuce (Lactuca sativa L.) [J]. Scientia Agricultura Sinica, 2017, 50(8): 1486-1494.
[5] ZHU Hong-long, YANG Jie, LI Jian, PAN Xiao-qing, QIN Feng, ZHOU Zhong-kai, FENG Guo-xing, GU Hong-ru. Comparative Analysis of Growth Performance, Behavior, and Salivary Cortisol Hormone of Piglets Housed in Two Rearing Environments [J]. Scientia Agricultura Sinica, 2016, 49(7): 1382-1390.
[6] LU Yang, HU Er-yong, ZI Zheng-hao, SUN Guo-rong, XIA Dong . Improvement of the Effects of Phytase Application by Lowering the High Level of Copper in Piglets Diets [J]. Scientia Agricultura Sinica, 2015, 48(14): 2884-2890.
[7] YANG Feng-juan, ZENG Xiang-fang, QIAO Shi-yan. Effect of Lactobacillus reuteri I5007 on Intestinal Morphology, Disaccharidase Activity and Tight Junction Protein Expression in Newborn Piglets [J]. Scientia Agricultura Sinica, 2014, 47(22): 4506-4515.
[8] JIANG Shu-zhen, SUN Hua, HUANG Li-bo, YANG Zai-bin, WANG Shu-jing, LIU Fa-xiao, F. Chi. Effects of Zearalenone Contaminated Diets on Serum Metabolite and Histopathology of Liver and Kidney in Weaned Piglets [J]. Scientia Agricultura Sinica, 2014, 47(18): 3708-3715.
[9] ZHAO Jiao, ZHOU Zhao-Hong, LIANG Xiao-Fang, MAO Xiang-Bing, CHEN Dai-Wen, YU Bing. Effects of GSPs and VE on Growth Performance, Serum Redox Status and Hepatic Oxidative Damage in Piglets Under Oxidative Stress [J]. Scientia Agricultura Sinica, 2013, 46(19): 4157-4164.
[10] ZHAO Li, CHEN Hao-Fei, ZHANG Wen-Bao, MA Zheng-Hai, ZHANG Zhuang-Zhi, ZHANG Xu, GU Nu-尔?Tu-尔Xun, MI Xiao-Yun, JIN Ying-Hong, XUE Jing, SHI Bao-Xin. Cloning, Prokaryotic Expression of Echinococcus granulosus Heat Shock Protein 70 and Preparation of It’s Antiserum [J]. Scientia Agricultura Sinica, 2012, 45(12): 2491-2501.
[11] ZHANG Xia; CAO Rong-feng; CONG Xia; LI Jin-bo; SUN Qi; GAO Shan-song; TIAN Wen-ru. Effect of CaM on Expression of Inducible Hsp70 in MEF [J]. Scientia Agricultura Sinica, 2011, 44(7): 1495-1501.
[12] FU Da-bo,WANG You-wei,HOU Yong-qing,DING Bin-ying,WANG Lei,LIU Yu-lan,ZHU Hui-ling
.

Effects of α-Ketoglutarate on Muscle Energy Metabolism in Weanling Pigs Chronically Challenged with Lipopolysaccharide

[J]. Scientia Agricultura Sinica, 2011, 44(4): 814-822 .
[13] ZHU Ye-meng,XIE Zheng-jun,LI Yun-tao,HAN Xin-yan
. Effects of Chitosan-Copper on Growth Performance and Intestinal Flora and Its Morphology in Weanling Piglets
 [J]. Scientia Agricultura Sinica, 2011, 44(2): 387-394 .
[14] LUO Jin, LIU Guang-Yuan, TIAN Zhan-Cheng, XIE Jun-Ren, ZHANG Ping, SHEN Hui, DANG Gen-Sheng. Taxonomic Status Assays of Theileria equi Based on Hsp70 Gene [J]. Scientia Agricultura Sinica, 2011, 44(17): 3694-3700.
[15] DING Yue-Yun, ZHOU Fen, ZHANG Wei, ZHANG Chen-Hua, YIN Zong-Jun. Effects of Astragalus, Angelica, Rhodiola on the Growth Performance and Some Physiological Functions of Weaning Piglets [J]. Scientia Agricultura Sinica, 2011, 44(16): 3469-3476.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd