Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (17): 3694-3700.doi: 10.3864/j.issn.0578-1752.2011.17.023

• RESEARCH NOTES • Previous Articles    

Taxonomic Status Assays of Theileria equi Based on Hsp70 Gene

LUO  Jin, LIU  Guang-Yuan, TIAN  Zhan-Cheng, XIE  Jun-Ren, ZHANG  Ping, SHEN  Hui, DANG  Gen-Sheng   

  1. 1.中国农业科学院兰州兽医研究所/家畜疫原病原生物学国家重点实验室/甘肃省动物寄生虫病重点实验室/农业部草食动物疫病重点开放实验室
    2.西安市长安区动物疾病预防控制中心
  • Received:2011-03-14 Revised:2011-05-23 Online:2011-09-01 Published:2011-07-10

Abstract: 【Objective】 The objective of the study is to determine the taxonomic status of Theileria equi (T. equi). 【Method】 According to the Hsp70 gene sequence (AB248743.1) of Babesia equi (B.equi) in GenBank, the primers TeHsp70F and TeHsp70R were designed. And a fragment with the length of 1 920 bp was obtained by PCR. The gene sequence was compared with other 23 amino acids sequences. 【Result】 The fragment encods 639 amino acids, 208 hydrophobic amino acids and 167 polar amino acid. Identity analysis showed that T. equi has a closest relationship with B.equi (BAF02625.1), and has a little relationship with T. parva (XP764717.1) and T. annulata (AAA30130.1), while T. equi has a distant relationship with B. caballi (BAF02619.1), which infects equine. 【Conclusion】 It was a correct taxonomic status for B. equi to Theileria species. And the taxonomic status was error before, for T. equi to Babesia species. In this paper, a proof was provided for taxonomic status of Theileria equi.

Key words: Theileria equi, Babesia caballi, Heat shock protein (Hsp70), taxonomic status

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