Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (7): 1392-1398.doi: 10.3864/j.issn.0578-1752.2012.07.018

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Effects of Different Dietary NFC/NDF Ratios on the Ruminal Fermentation and the Changes of the Rumen Microbial  Community of Goats

 WEI  De-Yong, ZHU  Wei-Yun, MAO  Sheng-Yong   

  1. 南京农业大学动物科技学院/消化道微生物实验室，南京 210095
  • Received:2011-04-15 Online:2012-04-01 Published:2011-07-29

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Abstract: 【Objective】This study was conducted to investigate the changes of the ruminal fermentation and the ruminal microbial composition as dietary NFC/NDF ratio increased.【Method】 Four local goats were gelded and designed for a self-controlled trial. The trial consisted of three periods corresponding to three diets with different NFC/NDF ratios (0.42，1.04，2.73). 【Result】The results showed that, as NFC/NDF ratio in the diet increased, the pH value decreased (P＜0.05), and the lactate, acetate, propionate, butyrate, isobutyric acid, valerate, isovalerate and TVFA concentration were increased (P＜0.05), and the ratio of the acetate to propionate decreased (P＜0.05). The analysis of DGGE profiles showed the sample with different NFC/NDF ratios diet fell into different groups. The real-time PCR results showed the copies of the 16S rRNA genes of Lactobacillus and total bacteria were affected by the dietary NFC/NDF ratio (P＜0.05), whereas no significant changes were found in the number of E.coli and the Streptococcus bovis (P＞0.05). 【Conclusion】 The results indicated that the characteristics of the ruminal fermentation were affected by the ratio of NFC/NDF in the diet, and with the increasing of the ratio of NFC/NDF, the ruminal fermentation was shifted from the acetate type to the propionate type, and the SARA (subacute rumen acidosis) occurred with the change of the ruminal microbiota when the ratio of the dietary NFC/NDF is 2.73.

Key words: carbohydrate structure, goat, ruminal fermentation, rumen microbial community

[1]Braun U, Rihs T, Schefer U. Ruminal lactic acidosis in sheep and goats. The Veterinary Record, 1992, 130(16): 343-349.

[2]Owens F N, Secrist D S, Hill W J, Gill D R. Acidosis in cattle: a review. Journal of Animal Science, 1998, 76: 275-286.

[3]Khafipour E, Krause D O, Plaizier J C. Alfalfa pellet-induced subacute ruminal acidosis in dairy cows increases bacterial endotoxin in the rumen without causing inflammation. Journal of Dairy Science, 2009, 92(4): 1712-1724.

[4]胡红莲,卢德勋,刘大程,珊丹,李胜利,张春华.日粮不同NFC/NDF比对奶山羊瘤胃pH、挥发性脂肪酸及乳酸含量的影响. 动物营养学报, 2010, 22(3): 595-601.

Hu H L, Lu D X, Liu D C, Shan D, Li S L, Zhang C H, Shi Y. Effects of different dietary NFC/NDF ratios on ruminal pH, VFA and lactate content in dairy goats. Acta Zoonutrimenta Sinica, 2010, 22(3): 595-601. (in Chinese)

[5]Nocek J E. Bovine acidosis: implications on lameness. Journal of Dairy Science, 1997, 80: 1005-1028.

[6]Yang W Z, Beauchemin KA. Physically effective fiber: method of determination and effects on chewing, ruminal acidosis, and digestion by dairy cows. Journal of Dairy Science, 2006, 89(7): 2618-2633.

[7]Batajoo K K, Shaver R D. Impact of nonfiber carbo-hydrate on intake, digestion, and milk production by dairy cows. Journal of Dairy Science, 1994, 77: 1580-1587.

[8]Wang M Z, Wang H R, Yu L H. Effect of NDF content on protozoal community and grazing rate in rumen. Journal of Animal and Veterinary Advances, 2009, 8(9): 1746-1752.

[9]Valizadeh R, Behgar M, Mirzaee M, Naserian A A, Vakili A R, Ghovvati S. The effect of physically effective fiber and soy hull on the ruminal cellulolytic bacteria population and milk production of dairy cow. Asian-Austria Journal of Animal Science, 2010, 23(10): 1325-1332.

[10]中华人民共和国农业行业标准—肉羊饲养标准(NY/T 34-2004). 中华人民共和国农业部. 北京: 中国农业出版社, 2004.

Agricultural Industry Standards of the People’s Republic of China-Feeding Standard of Meat-Producing Goats (NY/T34-2004). Ministry of Agriculture of the People’s Republic of China. Beijing: China Agriculture Press, 2004. (in Chinese)

[11]秦为琳. 应用气相色谱法测定挥发性脂肪酸的改进. 南京农学院学报, 1983, 5: 110-116.

Qin W L. Determination of rumen volatile fatty acids by means of gas chromatography. Journal of Nanjing Agricultural College, 1983, 3: 82-89. (in Chinese)

[12]Zoetendal E G, Akkermans A D L, de Vos W M. Temperature gradient gel electrophoresis analysis from human fecal samples reveals stable and host-specific communities of active bacteria. Applied and Environmental Microbiology, 1998, 64: 3854-3859.

[13]Mao S Y, Zhang G, Zhu W Y. Effect of disodium fumarate on ruminal metabolism and rumen bacterial communities in goat as revealed by denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA. Animal Feed Science and Technology, 2008, 140: 293-306.

[14]Su Y, Yao W, Perez-Gutierrez O N, Smidt H, Zhu W Y. 16S ribosomal RNA-based methods to monitor changes in the hindgut bacterial community of piglets after oral administration of Lactobacillus sobrius S1. Anaerobe, 2008, 14(2): 78-86.

[15]Muyzer G, de Waal E C, Uitterlinden A G. Profiling of complex microbiology population by denaturing gradient gel electrophoresis analysis of polymerase chain reaction amplified genes encoding for 16SrRNA. Applied Environmental Microbiology, 1993, 59: 695-700.

[16]Konstantinov S R, Smidt H, de Vos W M. Representational difference analysis and real-time PCR for strain-specific quantification of Lactobacillus sobrius sp. nov. Applied and Environmental Microbiology, 2005, 71(11): 7578-7581.

[17]Khafipour E, Li S, Plaizier J C, Krause D O. Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis. Applied and Environmental Microbiology, 2009, 75(22): 7115-7124.

[18]Beauchemin K A, Yang W Z, Rode L M. Effects of barley grain processing on the site and extent of digestion of beef feedlot finishing diets. Journal of Animal Science, 2001, 79: 1925-1936.

[19]Brown M S, Krehbiel C R, Galyean M L, Remmenga M D, Peters J P, Hibbard B, Robinson J, Moseley W M. Evaluation of models of acute and subacute acidosis on dry matter intake, ruminal fermentation, blood chemistry, and endocrine profiles of beef steers. Journal of Animal Science, 2000, 78: 3155-3168.

[20]Ghorbani G R, Morgavi D P, Beauchemin K A, Leedle J A. Effects of bacterial direct-fed microbials on ruminal fermentation, blood variables, and the microbial populations of feedlot cattle. Journal of Animal Science, 2002, 80: 1977-1986.

[21]Brossard L, Martin C, Michalet-Doreau B. Ruminal fermentaive parameters and bloodacido-basic balance changes during the onset and recovery of induced latent acidosis in sheep. Animal Research, 2003, 52: 513-530.

[22]Brossard L, Martin C. Protozoa involved in butyric rather than lactic fermentative pattern during latent acidosis in sheep. Reproduction Nutrition Development, 2004, 44: 195-206.

[23]张  迪. 慢性瘤胃酸中毒对瘤胃发酵功能及乳酸代谢菌的影响[D]. 呼和浩特: 内蒙古农业大学, 2008.

Zhang D. Effects of subacute rumen acidosis on rumen fermentation and populations of ruminal bacteria[D]. Huhhot: Inner Mongolia Agricultrual University, 2008. (in Chinese)

[24]Krause K M, Oetzel G R. Understanding and preventing subacute ruminal acidosis in dairy herds: a revie. Animal Feed Science and Technology, 2006, 126: 215-236.

[25]Khafipour E, Krause D O, Plaizier J C. A grain-based subacute ruminal acidosis challenge causes translocation of lipopolysaccharide and triggers inflammation. Journal of Dairy Science, 2009, 92(3): 1060-1070.

[26]常  影. 阉牛亚急性瘤胃酸中毒发病机理和饲喂高油玉米籽粒缓解效果的研究[D]. 北京: 中国农业大学, 2006.

Chang Y. Investigate on the mechinsm of subacute acidosis (SARA) and to evaluate the effect of feeding high oil corn grains on preventing or alleviating the development of SARA[D]. Beijing: China Agricultural University, 2006. (in Chinese)

[27]Khafipour E, Plaizier J C, Aikman P C, Krause D O. Population structure of rumen Escherichia coli associated with subacute ruminal acidosis (SARA) in dairy cattle. Journal of Dairy Science, 2011, 94(1): 351-360.

[28]Wells J E, Russell J B. Why do many ruminal bacteria die and lyse so quickly? Journal of Dairy Science, 1996, 79(8): 1487-1495.
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